Antioxidant and α-glucosidase inhibitory activity of Achillea tenorii

Context: There is a need for the discovery of novel natural remedies to prevent and treat metabolic disorders such as hyperglycemia, type II non-insulin-dependent diabetes mellitus, and obesity. Several Achillea species have been utilized for centuries all around the world and are generally considered effective as hypoglycemic. Objective: Considering the ethnobotanical uses of Achillea genus, we evaluated the in vitro inhibitory activity of Achillea tenorii Grande (Asteraceae) extract on a-glucosidase, which is a valuable target to prevent and treat metabolic disorders. We also tested its antioxidant activity. Moreover, the phytochemical profile was discussed from a chemotaxonomic point of view. Materials and methods: In vitro a-glucosidase inhibition of crude ethanolic extract obtained from the aerial parts was assayed as well as the in vitro antioxidant activity (ABTS, DPPH, and FRAP-FZ tests) was measured. The extract was characterized from a phytochemical point of view by means of spectroscopic analysis. Results: The extract results endowed with a-glucosidase inhibitory activity (IC50 32 mg/mL) with a particular mechanism of action definable as un-competitive, which differed from the mechanism observed for the best-known a-glucosidase inhibitor (acarbose and miglitol). In addition, a considerable antioxidant potential has been found for A. tenorii extract, which resulted mainly constituted by phenolic compounds such as caffeoylquinic acids and flavonoids. Discussion and conclusions: These results suggest the potential of A. tenorii as a possible natural remedy to prevent and treat metabolic disorders of carbohydrates

How a sensitive analysis on the coupling geology and borehole heat exchanger characteristics can improve the efficiency and production of shallow geothermal plants

Knowledge of the thermal behaviour around and throughout borehole heat exchangers (BHEs) is essential for designing a low enthalpy geothermal plant. In particular, the type of grout used in sealing the space between BHE walls and the pipes is fundamental for optimizing the heat transfer and minimizing the thermal resistance, thereby promoting the reduction of total drilling lengths and installation costs. A comparison between grouts with different thermal conductivities coupled with common hydrogeological contexts, was modelled for a typical one-year heating for continental climates. These data have been used for a sensitivity analysis taking into account different flow rates through pipes. The results highlight that in groundwater transient conditions, porous lithologies allow for greater heat power extractions to be obtained with an increasing grout thermal conductivity than limestone or clayey silt deposits do. Moreover, increasing the inlet flow rates through the pipe greatly improves the final heat power extraction. As a result, when the underground allows for high extraction rates, the use of high performing grouts is warmly suggested ensuring greater productions

Screening of ninety herbal products of commercial interest as potential ingredients for phytocosmetics

Elastase and tyrosinase are important targets both for cosmetics and for dermatological disorders. In this work, ninety herbal products were tested as inhibitors of these two enzymes. Eleven extracts resulted strongly active. Four out of them (Camellia sinensis, Ginkgo biloba, Rhodiola rosea, Vitis vinifera) inhibited both enzymes, five (Glycyrrhiza glabra, Ribes nigrum, Rheum officinale, Salvia officinalis, Tilia platyphyllos) were active against tyrosinase only, and two (Ceterach officinarum and Cinnamomum zeylanicum) proved selectively active against elastase. The IC50 ranged from 3.1 to 104.9 \u3bcg/mL and 19.3 to 164.3 \u3bcg/mL, against elastase and tyrosinase, respectively. The most active extracts resulted enriched in flavonoids (from 1.47 to 56.47 mg RE/g of extract) and phenolics (from 37.43 to 123.56 mg GAE/g of extract), indicating also an antioxidant potential. Finally, a positive correlation between enzymatic bioactivities and phenolic content was also established

Hybrid (Gas and Geothermal) Greenhouse Simulations Aimed at Optimizing Investment and Operative Costs: A Case Study in NW Italy

Generally, greenhouses are high energy-consuming, sometimes accounting for 50% of the cost of greenhouse production. Geothermal energy plays a very important role in maintaining the desired temperature and reducing energy consumption. This work deals with a project of a hybrid heating plant (97% geothermal energy and 3% gas-condensing boiler) for the innovative Plant Phenotyping Greenhouse at the University Campus in Grugliasco (few km West of the city of Turin). The aim of the study is to testify to the energy efficiency of this kind of hybrid plant as well as its economic sustainability. Numerical simulations of a GRT were used to calibrate the system and verify that the software reasonably modeled the real case. They helped to correctly size the geothermal plant, also providing data about the thermal energy storage and production during on and off plant cycles. The results show a thermal power of 50.92 kW over 120 days of plant operation, in line with the expected energy needs to meet the base load demand. Long-term results further ensure a negligeable impact on the ground, with a thermal plume between 5 and 10 m from the plant, reducing substantially in a few months after switching off the plant

GC-MS-based metabolomics for the detection of adulteration in oregano samples

Oregano is one of the most used culinary herb and it is often adulterated with cheaper plants. In this study, GC-MS was used for identification and quantification of metabolites from 104 samples of oregano (Origanum vulgare and O. onites) adulterated with olive (Olea europaea), venetian sumac (Cotinus coggygria) and myrtle (Myrtus communis) leaves, at five different concentration levels. The metabolomics profiles obtained after the two-step derivatization, involving methoxyamination and silanization, were subjected to multivariate data analysis to reveal markers of adulteration and to build the regression models on the basis of the oregano-to-adulterants mixing ratio. Orthogonal partial least squares enabled detection of oregano adulterations with olive, Venetian sumac and myrtle leaves. Sorbitol levels distinguished oregano samples adulterated with olive leaves, while shikimic and quinic acids were recognized as discrimination factor for adulteration of oregano with venetian sumac. Fructose and quinic acid levels correlated with oregano adulteration with myrtle. Orthogonal partial least squares discriminant analysis enabled discrimination of O. vulgare and O. onites samples, where catechollactate was found to be discriminating metabolite

Metabolomics and genetics of reproductive bud development in Ficus carica var. sativa (edible fig) and in Ficus carica var. caprificus (caprifig): similarities and differences

In figs, reproductive biology comprises cultivars requiring or not pollination, with female trees (edible fig) and male trees (caprifig) bearing different types of fruits. Metabolomic and genetic studies may clarify bud differentiation mechanisms behind the different fruits. We used a targeted metabolomic analysis and genetic investigation through RNA sequence and candidate gene investigation to perform a deep analysis of buds of two fig cultivars, 'Petrelli' (San Pedro type) and 'Dottato' (Common type), and one caprifig. In this work, proton nuclear magnetic resonance (H-1 NMR-based metabolomics) has been used to analyze and compare buds of the caprifig and the two fig cultivars collected at different times of the season. Metabolomic data of buds collected on the caprifig, 'Petrelli', and 'Dottato' were treated individually, building three separate orthogonal partial least squared (OPLS) models, using the "y" variable as the sampling time to allow the identification of the correlations among metabolomic profiles of buds. The sampling times revealed different patterns between caprifig and the two edible fig cultivars. A significant amount of glucose and fructose was found in 'Petrelli', differently from 'Dottato', in the buds in June, suggesting that these sugars not only are used by the ripening brebas of 'Petrelli' but also are directed toward the developing buds on the current year shoot for either a main crop (fruit in the current season) or a breba (fruit in the successive season). Genetic characterization through the RNA-seq of buds and comparison with the literature allowed the identification of 473 downregulated genes, with 22 only in profichi, and 391 upregulated genes, with 21 only in mammoni