2,667 research outputs found
mtDNA diversity in rabbit population from Sicily (Italy)
The European rabbit Oryctolagus cuniculus (O.c) lives all over the world and it represents an important resource for many predators. It has been classified as a Near-Threatened species in the Red List of Vertebrates of Italy. It is present in mediterranean basin as two known subspecies: O.c. cuniculus and O.c. algirus. The mediterranean geographic distribution of the two subspecies is still not well known. In particular, in Sicily, lacking of deep studies, is based on the body size and morphological characteristics; there wasn’t a complete description of the actual existing subspecies and previous studies only reported the morphological characteristics of the sicilian rabbit population. In this study, we analyzed genetic data, mitochondrial (mt) cytochrome b (cytb), from the rabbit population in Sicily in a phylogenetic framework. This is the first study concerning the genetics of the sicilian rabbit, to reconstruct intraspecific phylogeny by comparing cytb mtDNA sequences of 13 newly isolated O.cuniculus haplotypes from Sicily and 7 individuals from other countries (Canada, France, Mexico, North Italy, South Africa, Spain, Sweden). Our results show that the rabbit population from Sicily has a mitochondrial type (Lineage B) that has been previously shown to be associated with O. c. cuniculus and is similar to sequences from rabbits in North-Est Spain, Southern France, Sweden and South Africa
The Effects of Non-Indigenous Macrophytes on Native Biodiversity: Case Studies from Sicily
Biological invasions are widely recognized as a major threat to native biodiversity, ecosys tem functioning and services. Non-indigenous species (NIS) may in time become invasive (invasive alien species (IAS)), determining significant environmental, socioeconomic and human health impacts such as biodiversity loss and ecosystem service degradation. The Mediterranean islands, particu larly Sicily and the circum-Sicilian islands (northwestern Mediterranean Sea), which are important hotspots of biodiversity, are notably vulnerable to anthropogenic pressures such as biological in vasions. Therefore, monitoring NIS distribution as well as understanding their effects on native
biodiversity is critical in these areas for planning effective conservation strategies. Here, we report four different case studies from Sicily that highlight how NIS may affect native biodiversity and habitats. The first three case studies were carried out within Marine Protected Areas (MPAs) and highlight (1) the ability of Caulerpa cylindracea to promote the establishment of other NIS, including biofouling worms belonging to the genus Branchiomma; (2) how the shift in habitat from the native Ericaria brachycarpa to the invasive Asparagopsis taxiformis may drastically erode the primary producer biomass and associated biodiversity; and (2) that the presence of Lophocladia lallemandii can affect the
molluscan assemblage inhabiting the canopy-forming Gongolaria montagnei. The fourth case study, performed along the northwestern coast of Sicily, shows how Halophila stipulacea can affect the growth of the co-occurring native seagrass Cymodocea nodosa. Overall, these case studies demonstrate various ways in which NIS can interact with native biodiversity and habitats. Furthermore, they emphasize that MPAs are ineffective at preventing the introduction and spread of NIS
Conserving Marine Forests: Assessing the Effectiveness of a Marine Protected Area for Cystoseira sensu lato Populations in the Central Mediterranean Sea
Marine Protected Areas (MPAs) are vital for biodiversity conservation, yet their effectiveness in preserving foundation seaweeds remains understudied. This study investigates the diversity and distribution of Cystoseira sensu lato (including Cystoseira, Ericaria and Gongolaria, hereafter referred to as Cystoseira s.l.) populations in an MPA located in the central Mediterranean Sea, comparing them with those in two unprotected sites. We hypothesized MPA Cystoseira s.l. populations would display higher diversity and structure compared to outside unprotected sites. Results revealed a total of 19 Cystoseira s.l. species at depths of 0-20 m, with the MPA exhibiting a higher diversity than unprotected sites. Thus, MPAs can play a crucial role in fostering the diversity of Cystoseira s.l. populations. However, no significant differences were observed among the MPA's protection zones, raising questions about the zoning effectiveness. Additionally, our survey uncovered a substantial presence of non-indigenous seaweeds within the MPA. In conclusions, while MPAs improved Cystoseira s.l. diversity compared to unprotected sites, the varying efficacy of protection within MPA zones suggested a necessity for site-specific conservation strategies. The presence of non-indigenous seaweeds emphasizes ongoing challenges. This study provides a baseline for understanding Cystoseira s.l. population dynamics, crucial for future monitoring and conservation efforts in the face of global change
Capsular closure after hip arthroscopy: our experience
BACKGROUND AND AIM OF THE WORK: In the last decade, arthroscopic treatment of hip diseases has significantly spread and evolved and currently it represents the gold standard for the treatment of femoral- acetabular impingement. In the recent years, the function of the joint capsule (and therefore the results of an arthroscopic capsulotomy) has been hugely developed, opening a heated debate. The Literature is still torn about the need for a capsular suture, but more recent studies are more oriented in its execution at the end of the surgical procedure. According to these recent studies, the joint capsule performs an essential function of primary stability, and its closure is therefore necessary to restore the native anatomy and physiology. Nevertheless, capsular management remains a controversial topic. This is a retrospective study with the aim of assessing the influence of capsular suture on the patient's functional outcome in a cohort of patients with femoral-acetabular impingement arthroscopically treated. HYPOTHESIS: Our hypothesis is that an adequate capsular suture positively influences the patient's functional outcome. METHODS AND RESULTS: 50 patients treated with hip arthroscopy for femoral-acetabular impingement have been retrospectively enrolled at the Orthopaedic Clinic of Academic Hospital of Udine during a period of two-years (2017-2018); collected data have been analysed and compared with a retrospective model. Patients have been divided into two equivalent groups, 25 treated with capsular suture, 25 without performing the suture. Patient's post-operative functional outcome has been analysed using the modified Harris Hip Score (mHHS), the Non-Arthritic Hip Score (NAHS) and the Hip Outcome Score-Sport Scale (HOS-SS). The functional outcome in patients where capsular sutures were performed was better than in non-sutured patients, in all three analysed scales. CONCLUSIONS: Capsular suture with a single side-to-side stitch at the end of the procedure can positively influence the patient's functional outcome
Total knee revision arthroplasty: comparison between tibial tubercle osteotomy and quadriceps snip approach. Complication rate
BACKGROUND AND AIM OF THE WORK: The total knee arthroplasty (TKA) revision is not a second time of primary implant surgery but is a very complex issue for orthopedic surgeon. When local conditions make necessary a greater visualization, medial para-patellar access with quadriceps snip (QS) or the osteotomy of the tibial tuberosity (TTO) can be the solutions. This work aims to compare the quadriceps snip and the detachment of the tibial tubercle, focusing on possible complications. MATERIALS AND METHODS: At our institution, between January 2017 and February 2019 52 TKA revision for periprosthetic joint infection (PJI) or aseptic mobilization were performed. In 43 cases an extensive surgical approach was required: for patients with range of movement (ROM) 60\ub0 a QS was performed. Clinical and radiological follow-up was available for all the 43 cases. RESULTS: The data about clinical outcome in our study show that both groups have a positive trend in KSS score over time with similar ROM results. Two partial avulsions of patellar tendon during revision surgery were reported. Clinical outcome in both groups has shown good results at the end of follow-up with no post-operative incidence of complications. CONCLUSION: We can assert that both QS and TTO are good approach for TKA revision. Future studies will be needed to understand if preparatory ROM is a good way to decide which surgical approach to use.(www.actabiomedica.it)
Histological analysis of ACL reconstruction failures due to synthetic-ACL (LARS) ruptures
INTRODUCTION: Anterior Cruciate Ligament (ACL) reconstruction is an established surgical procedure. Synthetic ligaments represent an option for ACL reconstruction. Their popularity declined for the raising concerns due to re-ruptures, knee synovitis and early arthritis related to I and II generation artificial ligaments. The introduction of a III generation synthetic ligament (Ligament Advanced Reinforcement System-LARS) permitted renewed interest in the adoption of this kind of graft. Main purpose of our study was to describe the histological findings on samples obtained from a consecutive series of ACL revision surgeries due to LARS ACL reconstruction failures. Secondary aim was to determine the reason for LARS rupture. METHODS: In a period between 2016 and 2018 eleven patients underwent ACL revision surgery due to LARS ACL reconstruction failure. At the time of the arthroscopic procedure, samples of synovial membrane and remnants of the torn LARS were sent to the Pathological-Anatomy Institute of our Hospital for a histological analysis. RESULTS: Histological analysis of the synovial tissues confirmed the arthroscopic evidence of synovitis mainly characterized by chronic inflammation with predominance of multinucleated giant cells. The adoption of polarized light microscopy revealed the presence of brightly bi-refractive material (LARS wear particles) in the synovial tissue; at higher magnification wear debris were detected inside the cytoplasma of multi nucleated cells. The histological analysis of the removed LARS revealed a surrounding typical foreign body reaction with poor signs of fibrovascular ingrowth of the synthetic ligament. CONCLUSIONS: Our findings could not clearly advocate a unique mechanism of LARS-ACL reconstruction failure: biologic issues (poor tissue ingrowth) and mechanical issues (fibers properties and tunnel position) probably concur in a multi factorial manner. ACL reconstruction using artificial ligaments can not be considered a simple surgery. Artificial augments require some expertise and could therefore achieve better results if used by skilled sport surgeons other than trainees or low volume surgeons. The Authors believe that ACL reconstruction with synthetic devices still have restricted indications for selected patients (e.g. elderly patients who require a fast recovery, professional athlete, autologous tendons not available and/or refusing donor tendons). Our study arises additional suspicion on the unresponsiveness of synthetic fibers and claim some concern in the implantation of synthetic devices
effect of a nicotine free inhalator as part of a smoking cessation programme
Smoking-cessation drugs are inadequate at addressing the behavioural component of tobacco dependence. Nicotine-free inhalators are plastic devices that may provide a coping mechanism for conditioned smoking by replacing some of the rituals associated with smoking gestures. This study assessed the effect of using a nicotine-free inhalator to improve success in a cessation programme. At baseline, 120 smokers attending a smoking-cessation programme were assessed for their sociodemographic factors, smoking history, depression, physical and behavioural dependence, and motivation. Participants were randomly assigned to two groups, nicotine-free inhalator group (PAIPO; Echos Srl, Milan, Italy) versus reference group. For the whole sample, no significant difference was found in quit rates at 24 weeks between the PAIPO group and the reference group. However, the quit rate in the PAIPO group (66.7%) was more than three-fold higher than the reference group (19.2%) for those individuals with high Glover–Nilsson Smoking Behavioural Questionnaire (GN-SBQ) scores at baseline. The results of the logistic model analysis indicate that a high GN-SBQ score is a strong independent predictor for successful quitting at 24 weeks (OR 8.88; 95% CI 2.08–37.94) in the PAIPO group. Nicotine-free inhalators may be beneficial when used in the context of smoking-cessation interventions, particularly for those smokers for whom handling and manipulation of their cigarettes plays an important part in the ritual of smoking
Low Dose of Dipotassium Glycyrrhizate Counteracts Atherosclerosis Progression in Apoe-/- Female Mice
Preliminary studies on red palm weevil as potential risk for palm trees in mediterranean basin
The impacts of insect invasive species on human health and on agricutlture have attracted worldwide attention. However, researchers and policymakers rarely directly address the connection between invasive species and loss in natual and cultural heritages. Various attempts have been made to address the plants that are affected by invasive species but the links between these and loss in naturla biodiversity and heritage in botanical and historical gardens are largely lacking in the literature.
We report a study on the Red Palm Weevil (RPW), Rhynchophorous ferrugineus (Oliv.) (Fig. 1) (Coleoptera, Curculionidae) which is the most important pest of the palm date trees in many countries. RPW is a large polyphagous insect native to southern Asia and Melanesia and one of the most important pests of several palm species. It reached Italy in 2004 and now is wide spread in Sicily but also in almost all Mediterranean, through Phoenix dactylifera and P. canariensis (Fig. 2).
In Europe for the first time was established the RPW-P. canariensis interaction. As a result of RPW attack, the palm is destroyed and the tree collapses and dies.
A high density of RPW, developed in P. canariensis, is now present despite to the control measures, in Sicily. Palm trees represent in Mediterranean area an important part of cultural heritage in cities, botanical and historical gardens, which conserve many different palm species. In addition Mediterranean flora is also characterized by the presence of the native palm specie Chamerops humilis. In order to understand if the C. humilis is a suitable host of RPW, we investigate the natural infestation of RPW on this native palm. Moreover we analysed the morphological and molecular characters of RPW populations collected from C. humilis and P. canariensis. The study highlights the necessity to correlate the degree of genetic differences in the population of invasive species, as RPW, to predict the impact on natural flora and on other hosts
Rejection of randomly coinciding events in LiMoO scintillating bolometers using light detectors based on the Neganov-Luke effect
Random coincidences of nuclear events can be one of the main background
sources in low-temperature calorimetric experiments looking for neutrinoless
double-beta decay, especially in those searches based on scintillating
bolometers embedding the promising double-beta candidate Mo, because of
the relatively short half-life of the two-neutrino double-beta decay of this
nucleus. We show in this work that randomly coinciding events of the
two-neutrino double decay of Mo in enriched LiMoO
detectors can be effectively discriminated by pulse-shape analysis in the light
channel if the scintillating bolometer is provided with a Neganov-Luke light
detector, which can improve the signal-to-noise ratio by a large factor,
assumed here at the level of on the basis of preliminary
experimental results obtained with these devices. The achieved pile-up
rejection efficiency results in a very low contribution, of the order of counts/(keVkgy), to the background counting rate
in the region of interest for a large volume ( cm)
LiMoO detector. This background level is very encouraging in
view of a possible use of the LiMoO solution for a bolometric
tonne-scale next-generation experiment as that proposed in the CUPID project
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