A clinical study to evaluate the efficacy of Khaakhasadi Lepa in Padadari (Crack Heel)

Padadari = Pada + Dari. Padadari is one among Kshudra Rogas. Sushruta, Madhavnidana and Bhavprakasha has explained Padadari as distinct disease. In modern, it is termed as crack heel. Padadari is a Vatapradhana Vyadhi. Vataprakopa leads to the symptoms as Padasputana, Vedana, Rukshata and Daha. Atichankramana and Vataprakopaka Ahara and Vihara are the main cause for Padadari. Khaakhasadi Lepa has Vatahara, Vranashodhaka, Vranaropaka, Twagdoshahara, Kandughna properties which helps in Samprapti Vighatana of Padadari. The study is attempt to clinically analyze the efficacy of Khakhasadi Lepa indendently and compare with Moisturex cream in Padadari. This study is a randomized single blind standared control clinical study conducted on 60 subjects divided randomly in two groups. Group A (Trial group) and Group B (Control group) were formed with 30 patients in each group. Group A was treated with Khaakhasadi Lepa for one month. Group B was treated with Moisturex cream for one month. Lepa was adviced to apply in night on affected area of sole in quantity sufficient. Cleaning of sole and after proper drying, application of lepa was advised.The patients were assessed with severity of symptoms subjectively before and after treatment. Data from each group is statistically analyzed and compared. Both the group showed marked results but Khaakhasadi Lepa showed statistically highly significant result in subjective parameter like Vedana and Rukshata. Khakhasadi Lepa is not that much significant in Padasputana as compare to Moisturex cream. The study shows that Khaakhasadi Lepa and Moisturex cream had got equally significant relief in Vedana and Rukshata. Both drugs were insignificant in reducing Daha. Khakhasadi Lepa was not more effective in Padasputana

CONTROLLED RELEASE OF GABAPENTIN THROUGH RETICULATED CHITOSAN AND HYDROXYETHYL CELLULOSE HYDROGEL MATRIX TABLETS

The aim of the present work was to prepare hydrogel matrix tablets using crosslinked chitosan and hydroxyethyl cellulose (HEC) for controlled release of gabapentin. The chitosan was crosslinked with acetaldehyde and used for the preparation of hydrogel matrix tablets along with hydroxylethyl cellulose by wet granulation method. The samples were characterized by FTIR, DSC, TGA, XRD, SEM and evaluated drug content, swelling pattern and drug release. The matrix tablets were capable of releasing the drug for 24h depending upon the formulation variables. It was observed that as the concentration of HEC increased in the tablets, the drug release was also increased. On the other hand, as the crosslinking of chitosan was increased, the drug release was decreased. Drug release mechanism followed non-Fickian transport. This study demonstrated that the blend hydrogel matrix tablets of crosslinked chitosan and HEC could be a versatile drug delivery system for controlled release of gabapentin

Assessment of collagen fiber nature, spatial distribution, hue and its correlation with invasion and metastasis in oral squamous cell carcinoma and surgical margins using Picro Sirius red and polarized microscope

Introduction: Oral squamous cell carcinoma (OSCC) comprises a bulk of all the oral malignancies and is posing a major health problem among the population. It is an established fact that tumor stroma plays a vital role in tumor progression. Therefore, methods to detect, quantify, and analyze collagen are of immense value in this regard. Picro Sirius red, which has the capability to detect thin fibers, although frequently used, is seldom exploited to the fullest extent. Aim: Our goal is not only to identify nature of fibers, but also to assess the fiber hue and the spatial distribution of different colors in various grades of OSCC and correlate it with the metastasis of the tumor. The study has also analyzed the nature of stromal elements along the clear, close and involved surgical margins of OSCC. Materials and Methods: Ten cases each of well, moderately and poorly differentiated OSCC as well as clear, close and involved margins were stained with haematoxylin eosin and picrosirius red staining for evaluation under polarized microscope. Results: In this study we found that the birefringence of the collagen fiber changed from orange red to yellowish green from well to poorly differentiated OSCC. The collagen fibers in well-differentiated carcinoma revealed polarizing colors of reddish orange around the tumor islands in the majority of the fields. To the best of our knowledge is not being studied so far in the English literature. Conclusion: In the present study, it has been observed that stromal changes at the invading front of the tumor islands and with increasing grade of the tumor can be evaluated more efficiently with the use of Picro Sirius red stain