A study on the impact of AL-FEC techniques on TV over IP Quality of Experience

Abstract In this contribution, an evaluation of the effectiveness of Application Layer-Forward Error Correction (AL-FEC) scheme in video communications over unreliable channels is presented. In literature, several AL-FEC techniques for reducing the effect of noisy transmission on multimedia communication have been adopted. Recently, their use has been proposed for inclusion in TV over IP broadcasting international standards. The objective of the analysis performed in this paper is to verify the effectiveness of AL-FEC techniques in terms of perceived Quality of Service (QoS) and more in general of Quality of Experience (QoE), and to evaluate the trade-off between AL-FEC redundancy and video quality degradation for a given packet loss ratio. To this goal, several channel error models are investigated (random i.i.d. losses, burst losses, and network congestions) on test sequences encoded at 2 and 4 Mbps. The perceived quality is evaluated by means of three quality metrics: the full-reference objective quality metric NTIA-VQM combined with the ITU-T Rec. G.1070, the full-reference DMOS-KPN metric, and the pixel-wise error comparison performed by using the PSNR distortion measure. A post-processing synchronization between the original and the reconstructed stream has also been designed for improving the fidelity of the performed quality measures. The experimental results show the effectiveness and the limits of the Application Layer protection schemes

A study on the impact of AL-FEC techniques on TV over IP Quality of Experience

Abstract In this contribution, an evaluation of the effectiveness of Application Layer-Forward Error Correction (AL-FEC) scheme in video communications over unreliable channels is presented. In literature, several AL-FEC techniques for reducing the effect of noisy transmission on multimedia communication have been adopted. Recently, their use has been proposed for inclusion in TV over IP broadcasting international standards. The objective of the analysis performed in this paper is to verify the effectiveness of AL-FEC techniques in terms of perceived Quality of Service (QoS) and more in general of Quality of Experience (QoE), and to evaluate the trade-off between AL-FEC redundancy and video quality degradation for a given packet loss ratio. To this goal, several channel error models are investigated (random i.i.d. losses, burst losses, and network congestions) on test sequences encoded at 2 and 4 Mbps. The perceived quality is evaluated by means of three quality metrics: the full-reference objective quality metric NTIA-VQM combined with the ITU-T Rec. G.1070, the full-reference DMOS-KPN metric, and the pixel-wise error comparison performed by using the PSNR distortion measure. A post-processing synchronization between the original and the reconstructed stream has also been designed for improving the fidelity of the performed quality measures. The experimental results show the effectiveness and the limits of the Application Layer protection schemes

Mineralocorticoid Receptor in Novel Target Tissues: A Closer Look at the Adipocyte

In addition to the well-documented role in the kidney, the mineralocorticoid receptor (MR) has been recently identified in different “non-classical” target tissues, such as the brain, the heart, vasculature, macrophages/monocytes, and adipose tissue. In this context, the MR is involved in adipocyte fundamental processes such as differentiation, autophagy, and adipokine secretion. Excessive activation of the MR contributes to metabolic derangements occurring in mice with obesity and metabolic syndrome. Interestingly, MR pharmacological blockade in murine models of obesity has led to protection from weight gain and adipocyte dysfunctions. Unfortunately, there is still a lack of knowledge on the metabolic effects of MR antagonists, and larger clinical studies are deemed necessary to clarify the metabolic role of MR blockade in humans. This review discusses the role of MR in adipose tissue, focusing on regulation by MR of key cellular processes occurring in the adipocyte. The molecular pathways affected by MR activation or blockade in adipose tissue have been investigated only in part. Hence, more studies are necessary to get more insights in the role of aldosterone/MR in this “non-classical” target tissue and to better understand its potential implications in obesity and metabolic syndrome

Conformational equilibria in monomeric alpha-synuclein at the single molecule level

Natively unstructured proteins defy the classical "one sequence-one structure" paradigm of protein science. Monomers of these proteins in pathological conditions can aggregate in the cell, a process that underlies socially relevant neurodegenerative diseases such as Alzheimer and Parkinson. A full comprehension of the formation and structure of the so-called misfolded intermediates from which the aggregated states ensue is still lacking. We characterized the folding and the conformational diversity of alpha-synuclein (aSyn), a natively unstructured protein involved in Parkinson disease, by mechanically stretching single molecules of this protein and recording their mechanical properties. These experiments permitted us to directly observe directly and quantify three main classes of conformations that, under in vitro physiological conditions, exist simultaneously in the aSyn sample, including disordered and "beta-like" structures. We found that this class of "beta-like" structures is directly related to aSyn aggregation. In fact, their relative abundance increases drastically in three different conditions known to promote the formation of aSyn fibrils: the presence of Cu2+, the occurrence of the pathogenic A30P mutation, and high ionic strength. We expect that a critical concentration of aSyn with a "beta-like" structure must be reached to trigger fibril formation. This critical concentration is therefore controlled by a chemical equilibrium. Novel pharmacological strategies can now be tailored to act upstream, before the aggregation process ensues, by targeting this equilibrium. To this end, Single Molecule Force Spectroscopy can be an effective tool to tailor and test new pharmacological agents.Comment: 37 pages, 9 figures (including supplementary material

Fecal Microbiota, Bile Acids, Sterols, and Fatty Acids in Dogs with Chronic Enteropathy Fed a Home-Cooked Diet Supplemented with Coconut Oil

: Medium-chain fatty acids (MCFAs) are considered to be interesting energy sources for dogs affected by chronic enteropathies (CE). This study analyzed the clinical scores, fecal microbiota, and metabolomes of 18 CE dogs fed a home-cooked diet (HCD) supplemented with virgin coconut oil (VCO), a source of MCFA, at 10% of metabolizable energy (HCD + VCO). The dogs were clinically evaluated with the Canine Chronic Enteropathy Activity Index (CCECAI) before and at the end of study. Fecal samples were collected at baseline, after 7 days of HCD, and after 30 days of HCD + VCO, for fecal score (FS) assessment, microbial analysis, and determination of bile acids (BA), sterols, and fatty acids (FA). The dogs responded positively to diet change, as shown by the CCECAI improvement (p = 0.001); HCD reduced fecal fat excretion and HCD + VCO improved FS (p < 0.001), even though an increase in fecal moisture occurred due to HCD (p = 0.001). HCD modified fecal FA (C6:0: +79%, C14:0: +74%, C20:0: +43%, C22:0: +58%, C24:0: +47%, C18:3n-3: +106%, C20:4n-6: +56%, and monounsaturated FA (MUFA): -23%, p < 0.05) and sterol profile (coprostanol: -27%, sitostanol: -86%, p < 0.01). VCO increased (p < 0.05) fecal total saturated FA (SFA: +28%, C14:0: +142%, C16:0 +21%, C22:0 +33%) and selected MCFAs (+162%; C10:0 +183%, C12:0 +600%), while reducing (p < 0.05) total MUFA (-29%), polyunsaturated FA (-26%), campesterol (-56%) and phyto-/zoosterols ratio (0.93:1 vs. 0.36:1). The median dysbiosis index was <0 and, together with fecal BA, was not significantly affected by HCD nor by VCO. The HCD diet increased total fecal bacteria (p = 0.005) and the abundance of Fusobacterium spp. (p = 0.028). This study confirmed that clinical signs, and to a lesser extent fecal microbiota and metabolome, are positively influenced by HCD in CE dogs. Moreover, it has been shown that fecal proportions of MCFA increased when MCFAs were supplemented in those dogs. The present results emphasize the need for future studies to better understand the intestinal absorptive mechanism of MCFA in dogs

Effect of dietary inclusion of different lipid supplements on performance and carcass quality traits of growing beef heifers

The aim of this study was to evaluate the impact of dietary extruded flaxseed and/or rumen-protected lipids on growth performance and carcass quality of growing beef heifers. Sixty-three crossbreed heifers (Charolais X Limousine) were distributed into seven experimental groups, balanced in terms of age and live weight. Diets fed to the groups were isoproteic and differed in both, the dietary lipid source (extruded linseed and/or rumen-protected conjugated linoleic acid) and the supplementation length (90 or 180 days before slaughtering), having the same total amount of lipids and vitamin E, during their finishing period. The results obtained in the present study confirm that in low-protein diets, the inclusion of rumen-protected CLA, alone or in combination with flaxseed, did not bring any evident effect on feed intake, performance and carcass quality traits of growing beef heifers

Sildenafil Reduces Insulin-Resistance in Human Endothelial Cells

The efficacy of Phosphodiesterase 5 (PDE5) inhibitors to re-establish endothelial function is reduced in diabetic patients. Recent evidences suggest that therapy with PDE5 inhibitors, i.e. sildenafil, may increase the expression of nitric oxide synthase (NOS) proteins in the heart and cardiomyocytes. In this study we analyzed the effect of sildenafil on endothelial cells in insulin resistance conditions in vitro