Analysis of molecular diversity, population structure and linkage disequilibrium in a worldwide survey of cultivated barley germplasm (Hordeum vulgare L.)

BACKGROUND: The goal of our study was a systematic survey of the molecular diversity in barley genetic resources. To this end 953 cultivated barley accessions originating from all inhabited continents except Australia were genotyped with 48 SSR markers. Molecular diversity was evaluated with routine statistics (allelic richness, gene diversity, allele frequency, heterozygosity and unique alleles), Principal Coordinate Analysis (PCoA), and analysis of genome-wide linkage disequilibrium. RESULTS: A genotyping database for 953 cultivated barley accessions profiled with 48 SSR markers was established. The PCoA revealed structuring of the barley population with regard to (i) geographical regions and (ii) agronomic traits. Geographic origin contributed most to the observed molecular diversity. Genome-wide linkage disequilibrium (LD) was estimated as squared correlation of allele frequencies (r(2)). The values of LD for barley were comparable to other plant species (conifers, poplar, maize). The pattern of intrachromosomal LD with distances between the genomic loci ranging from 1 to 150 cM revealed that in barley LD extended up to distances as long as 50 cM with r(2 )> 0.05, or up to 10 cM with r(2 )> 0.2. Few loci mapping to different chromosomes showed significant LD with r(2 )> 0.05. The number of loci in significant LD as well as the pattern of LD were clearly dependent on the population structure. The LD in the homogenous group of 207 European 2-rowed spring barleys compared to the highly structured worldwide barley population was increased in the number of loci pairs with r(2 )> 0.05 and had higher values of r(2), although the percentage of intrachromosomal loci pairs in significant LD based on P < 0.001 was 100% in the whole set of varieties, but only 45% in the subgroup of European 2-rowed spring barleys. The value of LD also varied depending on the polymorphism of the loci selected for genotyping. The 17 most polymorphic loci (PIC > 0.80) provided higher LD values as compared to 19 low polymorphic loci (PIC < 0.73) in both structured (all accessions) and non-structured (European 2-rowed spring varieties) barley populations. CONCLUSION: A global population of cultivated barley accessions was highly structured. Clustering highlighted the accessions with the same geographic origin, as well as accessions possessing similar agronomic characters. LD in barley extended up to 50 cM, and was strongly dependent on the population structure. The data on LD were summarized as a genome-wide LD map for barley

Grain-structure development in heavily cold-rolled alpha-titanium

High-resolution electron back-scatter diffraction (EBSD) analysis was employed to establish mircostructure evolution in heavily cold-rolled alpha-titanium. After thickness reductions of 75% to 96%, significant microstructure and texture changes were documented. The surface area of high-angle grain boundaries was almost tripled, thus giving rise to an ultra-fine microstructure with a mean grain size of 0.6 μm. Moreover, orientation spread around typical ‘split-basal’ rolling texture substantially increased. These effects were suggested to be related to the enhancement of pyramidal 〈c+a〉 slip. Grain-structure development in heavily cold-rolled alpha-titanium (PDF Download Available

The nexus of soil radon and hydrogen dynamics and seismicity of the northern flank of the Kuril-Kamchatka subduction zone

The comparison of kinematics and dynamic parameters of radon and molecular hydrogen concentration in subsoil air on the stations network at the Petropavlovsk-Kamchatsky geodynamic proving ground with seismicity of the northern flank of the Kuril-Kamchatka subduction zone was fulfilled in the period from July till August 2004. On the basis of correlation analysis of the regional seismicity and variations of radon flux density calculated using the data of gas-discharge counters of STS-6 type and SSNTDs it was shown that the radon mass transfer abnormal variations are conditioned by both regional seismicity in total and the subduction zone of proving ground. The azimuths of «geodeformation waves» coming to the registration points are calculated during clearly expressed anomaly beginnings, which coincide with directions to earthquake epicenters taking place at the same time. The geochemical anomalies recorded are presumptively deformative by nature and can be conditioned by processes of «quasi-viscous» flow of the lithosphere during rearrangement of tectonic stress fields of the subduction zone. The short-term (predicted time Τ <14 days) precursor of the earthquakes swarm was revealed in hydrogen dynamics on August, 4-5 (four earthquakes had M≥5.3 and epicentral distance about 130 km from the Paratunka base station)

Design of Pre-Dumping Ring Spin Rotator with a Possibility of Helicity Switching for Polarized Positrons at the ILC

The use of polarized beams enhance the possibility of the precision measurements at the International Linear Collider (ILC). In order to preserve the degree of polarization during beam transport spin rotators are included in the current TDR ILC Lattice. In this report some advantages of using a combined spin rotator/spin flipper are discussed. A few possible lattice designs of spin flipper developed at DESY in 2012 are presented.Comment: Talk presented at the International Workshop on Future Linear Colliders (LCWS15), Whistler, Canada, 2-6 November 201

Evaluating the effectiveness of public health financing based on financial and non-financial indicators in terms of the knowledge economy

In conditions of the knowledge economy human capital, human value are the main factors of economic growth and prosperity of the state. Qualitative characteristics of human capital are indicators of public health. Therefore, one of the most important tasks of the state is to assess the effectiveness of financing public health expenditures. The methodological base of the research was the methods of system and economic analysis, mathematical statistics and decision optimization, which resulted in the identification of factors influencing the resource supply, availability and quality of public health. The formation of a comprehensive indicator of the effectiveness of the public health system based on the system of financial and non-financial indicators will allow to form numerically an assessment of the effectiveness of investments in public health and draw conclusions about the resources' provision, level of development, accessibility and quality of public health. The obtained results can be used in making effective public financial decisions, which allow to achieve an increase of accessibility and quality of health care, focusing on the human capital in conditions of knowledge economy.peer-reviewe

Vasa vasorum lumen narrowing in brain vascular hyalinosis in systemic hypertension patients who died of ischemic stroke

Ischemic stroke is a major cause of death among patients with systemic hypertension. The narrowing of the lumen of the brain vasculature contributes to the increased incidence of stroke. While hyalinosis represents the major pathological lesions contributing to vascular lumen narrowing and stroke, the pathogenic mechanism of brain vascular hyalinosis has not been well characterized. Thus, the present study examined the postmortem brain vasculature of human patients who died of ischemic stroke due to systemic hypertension. Hematoxylin and eosin staining and immunohistochemistry showed the occurrence of brain vascular hyalinosis with infiltrated plasma proteins along with the narrowing of the vasa vasorum and oxidative stress. Transmission electron microscopy revealed endothelial cell bulge protrusion into the vasa vasorum lumen and the occurrence of endocytosis in the vasa vasorum endothelium. The treatment of cultured microvascular endothelial cells with adrenaline also promoted the formation of the bulge as well as endocytic vesicles. The siRNA knockdown of sortin nexin-9 (a mediator of clathrin-mediated endocytosis) inhibited adrenaline-induced endothelial cell bulge formation. Adrenaline promoted protein-protein interactions between sortin nexin-9 and neural Wiskott–Aldrich syndrome protein (a regulator of actin polymerization). Spontaneously hypertensive stroke-prone rats also exhibited lesions indicative of brain vascular hyalinosis, the endothelial cell protrusion into the lumen of the vasa vasorum, and endocytosis in vasa vasorum endothelial cells. We propose that endocytosis-dependent endothelial cell bulge protrusion narrows the vasa vasorum, resulting in ischemic oxidative damage to cerebral vessels, the formation of hyalinosis, the occurrence of ischemic stroke, and death in systemic hypertension patients

The Role of Biologically Active Aqueous Humor Molecules of the Anterior Chamber and Lacrimal Fluid in the Implementation of the Hypotensive Effect of Non-Penetrating Deep Sclerectomy

To date, the factors affecting the course of the reparative process after non-penetrating deep sclerectomy (NPDS) have not been fully determined. There is no systematic information about the regulatory role of the cytokines TGF-β, IL-6, IL-8 and MMP-9, VEGF A 121 and 165 in the formation mechanisms of the newly created pathways consistency of intraocular aqueous humor outflow.Purpose. To determine possible ways of impact of biologically active aqueous humor molecules of the anterior chamber and lacrimal fluid on the hypotensive effect of non-penetrating deep sclerectomy.Methods. A prospective study of 65 patients with open-angle glaucoma before and 12 months after NPDS and 22 patients without eye hydrodynamic disorders with the determination of the initial concentrations of biologically active molecules in the lacrimal fluid and aqueous humor of the anterior chamber. Twelve months after NPDS all patients were divided into three groups, depending on the hypotensive effect of the operation, according to the criteria.Results. Multivariate discriminant analysis showed the greatest inter-group differences, calculated by the square of the Mahalanobis distance, between group 3 with no hypotensive effect of NPDS and the control group (R2 = 8.48, p = 0.001). The most informative features that determine the differences between the 4 groups in the total population, calculated according to the Fischer F-test, were MMP-9 (F = 14.7, p = 0.001) and TGF-β (F = 7.08, p = 0.001) in the aqueous humor of the anterior chamber. In pairwise comparison of groups 1 and 2, the maximum level of significance according to the F-criterion was characteristic of the level of tear IL-6 (F = 21.25, p = 0.001), with approximately equal degree – IL-8 (F = 7.85, p = 0.001) and VEGF (F = 7.12, p = 0.001), to a lesser extent TGF of aqueous humor (F = 4.43, p = 0.001) and MMR-9 (F = 2.23, p = 0.001). Between groups 1–3, the maximum differences according to the Fisher criterion were observed in the IL-8 (F = 20.99, p = 0.001), TGF (F = 8.75, p = 0.001) and to a lesser extent – TGF (F = 5.83, p = 0.001).Conclusion. The analysis of the obtained data showed the decisive role of the imbalance of proinflammatory cytokines, signaling proteins with prolymphoangiogenic activity, and MMP-9 in the aqueous humor of the anterior chamber, as well as in the initial state of the lacrimal fluid in the postoperative healing processes after NPDS

Experimental determination of the adhesive characteristics of the “elementary fiber-epoxy matrix” system

The paper considers the method of testing the elementary fiber-polymer matrix system. Results of determining the following adhesive characteristics are provided: apparent and local adhesive strength, start and end in the adhesive destruction of the fiber-matrix joint under study. Basalt fiber and epoxy matrix based on the epoxy resin and amine hardener are considered as the study objects. Influence of the samples geometric characteristics on their adhesive characteristics is established. The paper shows that at the depth of the elementary fiber emersion in the polymer matrix equal to 200 µm, the scatter of all determined adhesive characteristics is significantly reduced

The consciousness of the younger schoolboys of lexical variability: the opportunities of the Russian language teachers

В статье рассматривается процесс освоения младшими школьниками лексической многозначности и выявляются возможности учебников русского языка для начальной школы для осознания обучающимися этого явления. На основе анализа семантических ошибок при восприятии многозначных слов и данных эксперимента на толкование младшими школьниками многозначных слов описываются трудности освоения младшими школьниками семантической структуры многозначного слова и высказываются предположения о факторах, оказывающих влияние на этот процесс. Обсуждаются результаты анализа учебников русского языка для начальной школы В. П. Канакиной, В. Г. Горецкого с точки зрения возможностей для полноценного усвоения лексической многозначности. Сделаны выводы о необходимости систематической работы, направленной на освоение и осознание обучающимися лексической многозначности. Эта работа подразумевает создание условий для овладения разными значениями слова и установления взаимосвязей между этими значениями, помогающих школьнику осознать мотивированность производных значений многозначных слов на доступном для него языковом материале.T he article discusses the process of mastering by schoolchildren of lexical ambiguity and identifies the possibilities of Russian primary school textbooks for students to understand this phenomenon. Based on the analysis of semantic errors in the perception of multi-valued words and experimental data on the interpretation by younger schoolchildren of multi-valued words, difficulties are learned by younger schoolchildren on the semantic structure of a polysemantic word and suggestions are made about factors that influence this process. The results of the analysis of the textbooks of the Russian language for elementary school by V. P. Kanakina, V. G. Goretsky are discussed from the point of view of possibilities for the full-fledged mastery of lexical polysemy. The conclusions about the need for systematic work aimed at the development and awareness of students of lexical ambiguity. This work involves the creation of conditions for mastering different meanings of the word and establishing relationships between these meanings, helping the student realize the motivation of derived meanings of multi-valued words on the language material available to him

Clinical and Immunological Evaluation of the Effectiveness of Non-Penetrating Deep Sclerectomy in the Early Postoperative Period

Today the mechanisms of pathological scarring and inconsistency of the intraocular fluid outflow pathways after nonpenetrating deep sclerectomy (NPDS), reducing its effectiveness, have not yet been determined. There is no systematic information about the regulatory role of cytokine TGF-β and MMP-9 in the mechanisms of formation of the newly created intraocular fluid outflow pathways.Aim: to assess changes in the clinical and immunological status of patients with open-angle glaucoma after NPDS in the early postoperative period.Methods. A prospective examination of 26 patients with open-angle glaucoma before and in 1 month after NPDS with determination of concentration of TGF-β and MMP-9 in tear humor. One month after NPDS, all patients were divided into two groups depending on the effect of the operation achieved according to the developed criteria.Results. Before the operation, tears TGF-β values in group 1 exceeded baseline values of group 2 by 1.4 times, the initial content of MMP-9 in tears in both groups was comparable. After the operation, in group 1, a moderate increase in the concentration of tears TGF-β by 1.3 times and a significant increase in the concentration of tears MMP-9 in 4.6 times compared with baseline values were revealed. In patients of group 2, after NPDS, there was a significant increase in the concentration of tears TGF-β, on average, 2.4 times relative to preoperative values, the content of MMP-9 did not significantly change.Conclusion. 1 month after NPDS, an imbalance in the concentration of TGF-β and MMP-9 in tears was detected in patients of the two groups that have fundamental clinical differences in the hypotensive effect and the formation of postoperative intraocular fluid outflow pathways