AI MENTORING AS A FORM AKMEOLOGICHESKOJ ORIENTED PROFESSIONAL INTERACTION

Optimization of professional relationships in the "mentor- apprentice" promotes the formation of a professional orientation akmeologicheskoj both subjects interaction. On the one hand , mentoring is one of the oldest and most effective ways to acquire knowledge and skills of young employees of the company in the process of adapting to a new team . On the other - allows teacher to identify their professional and personal opportunities and consciously decide to choose the optimum time of interaction with the students, based on their individual characteristics, as well as improve their own skills.Оптимизация профессиональных взаимоотношений в системе «наставник – ученик» способствует формированию акмеологической профессиональной направленности у обоих субъектов взаимодействия. Наставничество является одним из самых старых и эффективных способов приобретения знаний и навыков молодыми сотрудниками предприятия в процессе их адаптации в новом коллективе; позволяет наставнику выявить свои профессионально-личностные возможности и осознанно определиться в выборе оптимального стажа взаимодействия с учениками, с учетом их индивидуальных особенностей, а также повысить уровень собственного мастерства

Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius

B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the impact of various culture medium components on biosynthesis of these proteinases. Yeast extract and gelatin did not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production increased to reach 140%. Biosynthesis of both serine proteinases, produced by B. intermedius at the late stationary phase, were found to be inhibited by individual amino acids, and to be insensitive to catabolite repression. In order to maximise enzyme production, the presence of Ca 2+ and Mg2+ at concentration of 5 mM was shown to be necessary. Based on the results of this work, the composition of a complex culture media for the effective production of late stationary phase proteinases by B. intermedius was developed

Picosecond Fluorescence Relaxation Spectroscopy of the Calcium-Discharged Photoproteins Aequorin and Obelin

Addition of calcium ions to the Ca2+-regulated photoproteins, such as aequorin and obelin, produces a blue bioluminescence originating from a fluorescence transition of the protein-bound product, coelenteramide. The kinetics of several transient fluorescent species of the bound coelenteramide is resolved after picosecond-laser excitation and streak camera detection. The initially formed spectral distributions at picosecond-times are broad, evidently comprised of two contributions, one at higher energy (25000 cm-1) assigned as from the Ca2+-discharged photoprotein-bound coelenteramide in its neutral state. This component decays much more rapidly (t1/2 2 ps) in the case of the Ca2+-discharged obelin than aequorin (t1/2 30 ps). The second component at lower energy shows several intermediates in the 150-500 ps times, with a final species having spectral maxima 19400 cm-1, bound to Ca2+-discharged obelin, and 21300 cm-1, bound to Ca2+-discharged aequorin, and both have a fluorescence decay lifetime of 4 ns. It is proposed that the rapid kinetics of these fluorescence transients on the picosecond time scale, correspond to times for relaxation of the protein structural environment of the binding cavit

Клиническая эффективность и безопасность применения ингаляционного простациклина у больных с инфекцией, вызванной SARS-CoV-2 (проспективное сравнительное исследование)

Aim. In this study we evaluated clinical effectiveness and safety of nebulized prostacyclin in patients with Novel Coronavirus Disease (SARS-CoV-2). Materials and methods: We have included 44 male patients with moderate PCR confirmed SARS-CoV-2 infection in this study. Control group consisted of 23 patients treated with nebulized prostacyclin (PGI2). besides standard therapy. We compared intensiveness and duration of infectious intoxication syndrome, duration of fever, cough as well as SpO2 level, complete blood count and chemokine status values. Results: Statistically significant difference in duration of fever, cough, intensiveness and duration of infectious intoxication syndrome were observed. Lymphocyte and platelet counts were significantly higher in control group We have also noticed significantly lower level of proinflammatory mediators and C4-complement component in control group. Only 1 adverse effect associated with inhaled prostacyclin was reported. Conclusion. Nebulized prostacyclin showed therapeutic efficacy and good safety profile in adults with moderate COVID-19.Цель: оценка клинической эффективности и безопасность ингаляционного простациклина у пациентов с новой коронавирусной инфекцией (SARS-CoV-2). Материалы и методы: в исследование были включены 44 пациента мужского пола с подтвержденной новой коронавирусной инфекцией среднетяжелого течения. Опытную группу составили 23 пациента, которым, помимо стандартной терапии, был назначен ингаляционный простациклин (PGI2). Клиническая эффективность илопроста была оценена по длительности и выраженности общеинфекционных синдромов (интоксикации, лихорадки), длительности кашля, уровню насыщения крови кислородом, значениям параметров общеклинического анализа крови, показателю иммунологического статуса пациентов. Результаты: получено статистически значимое снижение длительности лихорадки, продолжительности кашля, выраженности и длительности синдрома общей инфекционной интоксикации в опытной группе. Также отмечено, что у этих пациентов средние значения количества лимфоцитов, тромбоцитов достоверно увеличивалось, а значение СОЭ снижалось. Средние значения провоспалительных цитокинов, хемокинов, а также С4-компонента комплемента были статистически значимо ниже, чем у больных COVID-19 в группе сравнения. Нежелательные реакции, связанные с инга ляционной терапией простациклином, были отмечены в 1 наблюдении. Заключение: показана терапевтическая эффективность и хороший профиль безопасности ингаляционного простациклина у пациентов с COVID-19 средней степени тяжести

Voluntary exercise and testosterone therapy caused increase in percentage of Myh6 and expression of oxidative stress marker Cybb in left ventricles of rats

Aim: The aim of this study is to identify a possible damage to heart ventricles caused by supraphysiological doses of testosterone, voluntary physical activity or their combination

Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius

B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the impact of various culture medium components on biosynthesis of these proteinases. Yeast extract and gelatin did not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production increased to reach 140%. Biosynthesis of both serine proteinases, produced by B. intermedius at the late stationary phase, were found to be inhibited by individual amino acids, and to be insensitive to catabolite repression. In order to maximise enzyme production, the presence of Ca 2+ and Mg2+ at concentration of 5 mM was shown to be necessary. Based on the results of this work, the composition of a complex culture media for the effective production of late stationary phase proteinases by B. intermedius was developed

Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius

B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the impact of various culture medium components on biosynthesis of these proteinases. Yeast extract and gelatin did not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production increased to reach 140%. Biosynthesis of both serine proteinases, produced by B. intermedius at the late stationary phase, were found to be inhibited by individual amino acids, and to be insensitive to catabolite repression. In order to maximise enzyme production, the presence of Ca 2+ and Mg2+ at concentration of 5 mM was shown to be necessary. Based on the results of this work, the composition of a complex culture media for the effective production of late stationary phase proteinases by B. intermedius was developed

Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius

B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the impact of various culture medium components on biosynthesis of these proteinases. Yeast extract and gelatin did not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production increased to reach 140%. Biosynthesis of both serine proteinases, produced by B. intermedius at the late stationary phase, were found to be inhibited by individual amino acids, and to be insensitive to catabolite repression. In order to maximise enzyme production, the presence of Ca 2+ and Mg2+ at concentration of 5 mM was shown to be necessary. Based on the results of this work, the composition of a complex culture media for the effective production of late stationary phase proteinases by B. intermedius was developed

Experiments – Simulations – Theories: Multiscale approaches for solutions

The theory of complex media depends on various levels of description. At the macroscopic scale, hydrodynamic equations are used but they are not valid at small scales, the latter domain can be very important if slow processes occur in the system. At the microscopic scale, molecular dynamics describe the motion of the atoms individually. The two methods which correspond to different levels of description are complementary, but they are not the only ones. Intermediate methods based on continuous solvent model are able to take proper account of the solute dynamics and they can be applied at an intermediate time scale. We present such multi-scale descriptions in the case of solutions. Such an approach is all the more important in the context of of neutron science since neutron scattering experiments are now able to explore a large time domain: they are able to encompass the intermediate time domain for which intermediate methods are unavoidable