Caracterização Bioquímica e Farmacológica da Sp-ctx -toxina Multifuncional da Peçonha do Peixe Escorpião scorpaena Plumieri

Citolisinas são produzidas por diversos organismos e apresentam atividade tóxica a células individuais, causando dissolução através de lise. Citolisinas de peçonhas de peixes são proteínas multifuncionais que apresentam efeitos neurotóxicos, cardiotóxicos e inflamatórios, sendo descritas como fatores proteicos letais. Uma citolisina hemolítica lábil, formadora de poro, denominada Sp-CTx Scorpaena plumieri - Cytolytic Toxin (glicoproteína, 2 subunidades de 65 kDa), foi recentemente purificada da peçonha do peixe escorpião Scorpaena plumieri. O objetivo deste trabalho foi estabelecer condições ótimas de manutenção da atividade hemolítica de Sp-CTx e dar continuidade à sua caracterização bioquímica e farmacológica. A estabilidade da Sp-CTx em diferentes condições (pH, temperatura e presença de cosolutos) e o efeito de diferentes cátions e lipídios de membrana sobre a atividade hemolítica foram avaliadas em eritrócitos de coelho. Foi demonstrado que a atividade hemolítica da Sp-CTx é mantida quando armazenada em 500 mM de NaCl, 1 M de sulfato de amônio, 60 mM de trealose e 10% de glicerol em pH 7,4 a 4 ºC e em glicerol 10% a -80 e -196 ºC. Também foi visto que a atividade hemolítica da Sp-CTx é dependente de cálcio e é completamente inibida por EDTA. O colesterol e o fosfatidilglicerol reduziram a hemólise induzida pela toxina, o que sugere que a toxina forma poro do tipo barril e é dependente da interação eletrostática com lipídios de membrana. Os motivos de carboidratos da Sp-CTx foram avaliados por western blot usando o kit DIG Glycan Differentiation sugerindo a presença dos motivos: galactose (1-4) N-acetilglucosamina e ácido siálico (2-3) galactose em complexos de N- e/ou O-glicanos. A resposta inflamatória induzida pela Sp-CTx foi avaliada usando como modelo o teste da pata de camundongo. Sp-CTx induziu nocicepção e resposta edematogênica local dose-dependente. O edema induzido foi significativamente reduzido pela administração prévia de HOE-140 e de aprotinina. Estes dados evidenciam o envolvimento do sistema calicreína cinina na resposta inflamatória induzida por Sp-CTx

Does the use of playback affect the estimated numbers of red–legged partridge Alectoris rufa?

¿Afecta el uso de playback a las cifras estimadas de perdiz roja, Alectoris rufa? La perdiz roja, Alectoris rufa, vive en una situación de riesgo potencial en cuanto a su conservación a largo plazo en Italia, dado que su hábitat se ve cada vez más amenazado por la desaparición de los ambientes tradicionalmente relacionados con la agricultura. En tal situación, es importante aplicar métodos de control efectivos para estudiar los cambios poblacionales a través del tiempo, y la identificación de amenazas potenciales a la conservación. El objetivo de este estudio era evaluar la efectividad del método de playback para estimar la densidad de machos que vocalizaban. Comparamos el método del playback con las llamadas espontáneas de los machos al amanecer, y las observaciones directas a lo largo de transectos. El resultado de los datos sin procesar de los recuentos mediante playback revelaron una gran tasa de subestimación, comparados con el método que rindió los mejores resultados: el recuento de vocalizaciones espontáneas al amanecer. Nuestro estudio proporciona una evaluación crítica de un método que está ampliamente extendido, aunque los datos sobre su eficacia son escasos. Nuestros datos no evalúan la probabilidad de detección de los tres métodos. Nuestra intención era únicamente evaluar qué métodos arrojan los mejores resultados en términos de la estimación del tamaño de la población en las mismas condiciones de campo (misma densidad de población, mismo periodo, misma área de monitorización). Los resultados arrojan algunas dudas sobre la capacidad del método del playback para monitorizar las poblaciones de perdiz roja. Se discuten las implicaciones de nuestros resultados para su aplicación en la gestión de poblaciones de perdiz roja.¿Afecta el uso de playback a las cifras estimadas de perdiz roja, Alectoris rufa? La perdiz roja, Alectoris rufa, vive en una situación de riesgo potencial en cuanto a su conservación a largo plazo en Italia, dado que su hábitat se ve cada vez más amenazado por la desaparición de los ambientes tradicionalmente relacionados con la agricultura. En tal situación, es importante aplicar métodos de control efectivos para estudiar los cambios poblacionales a través del tiempo, y la identificación de amenazas potenciales a la conservación. El objetivo de este estudio era evaluar la efectividad del método de playback para estimar la densidad de machos que vocalizaban. Comparamos el método del playback con las llamadas espontáneas de los machos al amanecer, y las observaciones directas a lo largo de transectos. El resultado de los datos sin procesar de los recuentos mediante playback revelaron una gran tasa de subestimación, comparados con el método que rindió los mejores resultados: el recuento de vocalizaciones espontáneas al amanecer. Nuestro estudio proporciona una evaluación crítica de un método que está ampliamente extendido, aunque los datos sobre su eficacia son escasos. Nuestros datos no evalúan la probabilidad de detección de los tres métodos. Nuestra intención era únicamente evaluar qué métodos arrojan los mejores resultados en términos de la estimación del tamaño de la población en las mismas condiciones de campo (misma densidad de población, mismo periodo, misma área de monitorización). Los resultados arrojan algunas dudas sobre la capacidad del método del playback para monitorizar las poblaciones de perdiz roja. Se discuten las implicaciones de nuestros resultados para su aplicación en la gestión de poblaciones de perdiz roja.The red–legged partridge Alectoris rufa lives in a situation of potential conservation risk for its long–term preservation in Italy as its habitat is increasingly threatened by the disappearance of traditional agriculture–related environments. In such a situation, it is important to use effective and appropriate monitoring methods to assess population changes over time and to identify potential conservation threats. The objective of this study was to evaluate the effectiveness of the playback method to estimate the density of calling males. We compared playback method with spontaneous calling of males at dawn and direct observations along transects. The results on raw count data of playback counts revealed a strong underestimation rate compared to the method that gave the best results: count of spontaneous calls at dawn. Our study provides a critical evaluation of a method that is widely used even though data about its effectiveness are scarce. Our data do not evaluate detection probability of the three methods. Our aim was only to evaluate which methods give the best results in term of population size estimation under the same field condition (same population density, same period, same monitoring area). The results raise some doubts about the ability of the playback method to monitor red–legged partridge populations. The implications of our results for red–legged population management are discussed

UWB FastlyTunable 0.550 GHz RF Transmitter based on Integrated Photonics

Currently, due to the 6G revolution, applications ranging from communication to sensing are experiencing an increasing and urgent need of software-defined ultra-wideband (UWB) and tunable radio frequency (RF) apparatuses with low size, weight, and power consumption (SWaP). Unfortunately, the coexistence of ultra-wideband and software-defined operation, tunability and low SWaP represents a big issue in the current RF technologies. Recently, photonic techniques have been demonstrated to support achieving the desired features when applied in RF UWB transmitters, introducing extremely wide operation and instantaneous bandwidth, tunable filtering, tunable photonics-based microwave mixing with very high port-to-port isolation, and intrinsic immunity to electromagnetic interferences. Moreover, the recent advances in photonics integration also allow to obtain very compact devices. In this article, to the best of our knowledge, the first example of a complete tunable software-defined RF transmitter with low footprint (i.e. on photonic chip) is presented exceeding the state-of-the-art for the extremely large tunability range of 0.5-50 GHz without any parallelization of narrower-band components and with fast tuning (< 200 s). This first implementation represents a breakthrough in microwave photonics

Influence of feeding flavouring-appetizing substances on activity of cows in an automatic milking system

Aim of this research was to study the effect of somatic cell count on mineral content and salt equilibria at the level of quarter milk samples. Ten Italian Friesian cows, in which two homologous quarters (front quarters in 1 cow, rear quarters in 6 cows and both rear and front quarters in 3 cows) were characterised by a milk SCC400,000 cells/mL (HC-milk), respectively, were selected. Cows were milked at quarter level during the morning milking and a single sample was collected from each selected quarter, thus, 26 quarter milk samples were collected. Compared to LC-milk, HC-milk was characterised by a lower content of phosphorus and potassium and by a higher content of both sodium and chloride. The equilibrium of calcium, phosphorus and magnesium between the colloidal and soluble phase of milk and the mineralisation degree of the casein micelles, were not different between HC and LC milk

Photonics enabling coherent MIMO radar networks

The potential of coherent MIMO radar networks enabled by photonics is introduced. The first coherent dual-band 2 × 4 MIMO radar experiment is presented. Range/cross-range maps demonstrate the higher cross-range resolution due to the coherence and the enhanced performance introduced by dual-band operation

Photonics-based tunable 1-50 GHz RF transmitter on silicon chip

The paper presents an innovative tunable software-defined RF transmitter based on integrated photonics, able to work in 1-50GHz with very fast tuning. The system has been characterized and verified in a communication scenario

Carbapenem-Sparing Antibiotic Regimens for Infections Caused by Klebsiella pneumoniae Carbapenemase-Producing K. pneumoniae in Intensive Care Unit

A carbapenem-sparing regimen of tigecycline plus gentamicin or colistin was effective for treating 24 of 26 (92%) Klebsiella pneumoniae carbapenemase-producing K. pneumoniae infectious episodes in 22 polytrauma intensive care unit patients without comorbidities. The 30-day crude mortality rate was 14%. Regimens were considered appropriate in 12% of episodes according to the Vitek 2 System and in 100% based on E-test

Automatic Milking Systems in the Production of Parmigiano Reggiano Cheese: Effects on the Milk Quality and on Cheese Characteristics

The adoption of innovative processes, such as the automatic milking system (AMS), in the production of typical PDO cheeses, such as Parmigiano Reggiano, needs to be evaluated and tested, to verify its influence on milk quality and the typicality of the product. The present research was aimed to study the effect of the introduction of the AMS on the cheesemaking characteristics of the milk and the ripening process and the sensory properties of the Parmigiano Reggiano PDO cheese. Six cheesemaking trials were performed and, in each trial, two different separated pools of milk were submitted to the cheesemaking process in parallel, one from AMS and the other from a traditional milking parlor (TMP). AMS milk, in comparison with TMP one, showed higher content of lactose and calcium and lower contents of somatic cells, thermophilic lactic acid bacteria and chloride. Nevertheless, these changes were too small to influence the efficiency of the cheesemaking process and the cheese composition and its sensory profile. Moreover, cheeses made with AMS milk fully complied with the sensory characteristics of Parmigiano Reggiano cheese PDO, as defined by the PDO rules

Coherent dual-band 2x4 MIMO radar experiment exploiting photonics

In this paper the first coherent dual-band 2x4 MIMO radar experiment is presented. Range/cross-range maps demonstrate the higher cross-range resolution due to the coherence and the enhanced performance introduced by dual-band operatio