4 research outputs found
Concomitant visceral and localized cutaneous leishmaniasis in two Moroccan infants
Background: Leishmaniases are vector-borne diseases caused by the protozoa of the Leishmania genus. The clinical
spectrum of these diseases extends from benign dermal lesions to visceral forms. In the Mediterranean region,
zoonotic visceral leishmaniasis (ZVL) is caused by L. infantum. If untreated within two years, the disease usually
leads to death. In Morocco, ZVL is endemic in the north, with a hundred cases notified each year, mostly in
children aged below five years. Here, we report on two clinical observations in infants presenting unusual concomitant
VL and cutaneous leishmaniasis (CL) in Morocco.
Case presentation: In this case study, we report on two infants aged nine and 12 months old. They both have a
history of febrile splenomegaly, anemia, and pallor of mucous membranes. Visceral leishmaniasis was confirmed
by parasitological diagnosis (positive bone marrow smear and screening of anti-L. infantum antibodies). However,
the clinical examination also showed cutaneous lesions that suggested the presence of CL. This was reinforced by
the patients having a history of living or traveling to endemic foci. Thus, direct examination, culture, and PCR-RFLP (ITS1-
Hae 3) were carried out on the patients’ dermal exudates. In one of the infants, CL was associated with L. infantum, while
in the other it was associated with L. tropica. The infants were treated as according to the recommendations of
the Ministry of Health. Both patients were cured in two months; defervescence, reduction of splenomegaly, and
healing of cutaneous lesions were all observed.
Conclusions: These singular patients illustrate the clinical polymorphism of CL and the necessity of updating the
differential diagnosis of leukemia-like syndromes, including VL, in children living in or travelling to known endemic
areas. These observations suggest a change in the Mediterranean VL phenotype that may be associated with CL
Moroccan strains of Leishmania major and Leishmania tropica differentially impact on nitric oxide production by macrophages
International audienceBackgroundCutaneous leishmaniasis (CL) is a vector-borne parasitic disease caused by protozoa of the genus Leishmania. In Morocco, CL is a public health problem mainly caused by Leishmania major and Leishmania tropica, which are responsible for zoonotic and anthroponotic CL, respectively. Macrophages are the primary cells infected by Leishmania parasites and their capacity to produce nitric oxide (NO) is of critical importance for parasite elimination. To our knowledge, the role of NO on autochthonous infections has never been investigated before. In this study, we evaluated in vitro the capacity of autochthonous primary dermotropic strains of L. major and L. tropica to modulate NO production by J774-macrophages and determine the sensitivity of both species to exogenous NO.MethodsThe infectivity of the J774 cell line was analyzed by optical microscopy. NO production by macrophages was measured by the Griess method. The sensitivity to NO by the two strains was assessed by the MTT assay using NO donors.ResultsOur results show that the percentage of infected macrophages and the average number of parasites per macrophage were similar for L. major and L. tropica strains. While L. tropica significantly inhibited NO production induced by LPS and IFN-Îł stimulation in J774 macrophages, L. major did not affect it. However, soluble Leishmania antigens (SLAs) from both autochthonous primary strains significantly inhibited the production of NO by J774-macrophages in a dose-dependent manner. Finally, our results demonstrated that promastigotes and amastigotes from both strains are sensitive to SNAP NO donor in a dose-dependent manner, although L. tropica demonstrated an increased sensitivity.ConclusionsOur results suggest a differential ability of L. major and L. tropica strains to modulate the capacity of macrophages to produce NO. The increased ability of L. tropica to inhibit NO production by macrophages might come as a necessity due to its higher sensitivity to NO donor. Our results provide one explanation for the tendency of L. tropica to cause chronic lesions and may contribute to the different physiopathology of CL in Morocco
The Modulation of NADPH Oxidase Activity in Human Neutrophils by Moroccan Strains of Leishmania major and Leishmania tropica Is Not Associated with p47phox Phosphorylation
International audiencePolymorphonuclear neutrophils (PMNs) are the first phagocyte recruited and infected by Leishmania. They synthetize superoxide anions (O2-) under the control of the NADPH oxidase complex. In Morocco, Leishmania major and L. tropica are the main species responsible for cutaneous leishmaniasis (CL). The impact of these parasites on human PMN functions is still unclear. We evaluated the in vitro capacity of primary Moroccan strains of L. major and L. tropica to modulate PMN O2- production and p47phox phosphorylation status of the NADPH oxidase complex. PMNs were isolated from healthy blood donors, and their infection rate was measured by microscopy. O2- production was measured by superoxide dismutase-inhibitable reduction of cytochrome C. P47phox phosphorylation was analyzed by Western blot using specific antibodies against Ser328 and Ser345 sites. Whereas we did not observe any difference in PMN infectivity rate, our results indicated that only L. tropica promastigotes inhibited both fMLF- and PMA-mediated O2- production independently of p47phox phosphorylation. Leishmania soluble antigens (SLAs) from both species significantly inhibited O2- induced by fMLF or PMA. However, they only decreased PMA-induced p47phox phosphorylation. L. major and L. tropica modulated differently O2- production by human PMNs independently of p47phox phosphorylation. The inhibition of ROS production by L. tropica could be a mechanism of its survival within PMNs that might explain the reported chronic pathogenicity of L. tropica CL
Additional file 1: of Concomitant visceral and localized cutaneous leishmaniasis in two Moroccan infants
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