Revisited the relationship between vitamin D level and receptors of BsmI-gene polymorphism with the pathogenetic mechanisms of placental dysfunction development

The role of the calcitriol / vitamin D receptor (VD) endocrine system and the pleiotropic effects of this system in the pathogenetic mechanisms of various diseases development, in particular complications of pregnancy, has attracted researches’ increasing attention in recent years. The aim of the work: to compare the VD-status and frequency of occurrence of polymorphism of the VDR gene (BsmI (A> G, rs1544410) in patients with a physiological course of the gestation process and in patients with placental dysfunction (PD). Materials and methods. 56 pregnant women with PD (the main group) and 40 patients with a physiological pregnancy (control group) were examined. VD status was determined by ELISA at level 25 (OH) D in serum, the frequency of BsmI polymorphism of the VDR gene (rs1544410) by polymerase chain reaction (PCR). Results. The average index of VD (31.40 ± 8.6) ng / ml in patients with PD is significantly lower than in patients with physiological pregnancy (43.54 ± 11.20) ng / ml, (p ≤ 0.05 ). In patients with PD, homozygous carrier for the A-allele was found in 12% of cases, in healthy pregnant women - in 16.7%, (р ≥ 0.05), for the G-allele - in 20% and 47.20%, (р ≤ 0.01) cases, respectively to groups. Heterozygous combination of A / G alleles was noted in 68% of patients with PD and in 36.10% of the control group patients. In pregnant women with BsmI polymorphism of calcitriol gene (genotype A / G) PD was 3.7 times more frequent (68% vs 36.10% : RR = 2.1, CI 1.0-6.6, OR = 3.7, CI 1.1-13.1). Conclusions. Vitamin D insufficiency or deficiency can be one of the reasons of PD formation. In carriers of BsmIgene’s polymorphism encoding VD receptor with genotype A / G, the course of pregnancy is complicated by placental dysfunction 3.7 times more often than in women without this polymorphism

Molecular-genetic aspects of the endometrium state on the day of the tentative implantation window in women with recurrent miscarriage in the programs of assisted reproductive technologies

Golovatyuk K. P., Nosenko O. M., Makshayeva E. T., Trofimov D. Yu., Donnikov A. E., Kolin V. V. Molecular-genetic aspects of the endometrium state on the day of the tentative implantation window in women with recurrent miscarriage in the programs of assisted reproductive technologies. Journal of Education, Health and Sport. 2017;7(9):621-626. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1068361 http://ojs.ukw.edu.pl/index.php/johs/article/view/5076 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2017; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 15.08.2017. Revised: 20.09.2017. Accepted: 29.09.2017. MOLECULAR-GENETIC ASPECTS OF THE ENDOMETRIUM STATE ON THE DAY OF THE tentative IMPLANTATION WINDOW IN WOMEN WITH recurrent miscarriage IN THE PROGRAMS OF ASSISTED REPRODUCTIVE TECHNOLOGIES 1K. P. Golovatyuk, 2O. M. Nosenko, 1E. T. Makshayeva, 3D. Yu. Trofimov, 4A. E. Donnikov, 3V. V. Kolin 1Limited Liability Company Medical Center for Reproductive Health "Gameta", Odessa, Ukraine; 2Odessa National Medical University, Ukraine; 3Limited Liability Company "NPF DNA-Technology", Moscow, Russia; 4FGBU Scientific Center for Obstetrics, Gynecology and Perinatology. named after acad. V.I. Kulakov Ministry of Health care and Social Development of Russia, Moscow, Russia; e-mail: [email protected] Abstract More than 50% of pregnant women after the programs of assisted reproductive technologies (ART) face the problem of recurrent miscarriage (RMC), especially in the first trimester. Significant role in the development of RMC has infectious factor and chronic inflammation in the endometrium. The aim: to reveal the peculiarities of immune response mRNA genes of the inflammatory component expression in the period of the tentative implantation window (TIW) in women with RMC in ART programs. Material and methods. The main group consisted of 240 patients with RMC in ART programs; the control group included 100 conditionally healthy fertile women. On the ground of PCR reverse transcription, the mRNA of the IL-1β, IL-2, IL-10, Foxp3, TLR9, IL-2Rα cytokine genes was examined in endometrial samples obtained with the help of biopsy on the TIW day. Results. Analysis of the transcriptional profile of the immune response genes in the endometrium on TIW day revealed that the relative level of mRNA expression of the IL-1β, IL-2, Foxp3, TLR9, IL-2Rα genes did not differ significantly in the main and control groups. Statistically significant decrease in mRNA expression of IL-10 gene was observed in women with RPL. Conclusions. A feature of mRNA expression of the inflammatory component of the immune response in TIW period in women with RMC in ART programs is a decrease in the expression level of the IL-10 gene mRNA, which may be one of the reasons for the unfavorable outcomes of the onset pregnancy. Key words: recurrent miscarriage, assisted reproductive technologies, immune response, reverse transcription-polymerase chain reaction, tentative implantation window

Revisited the relationship between vitamin D level and receptors of BsmI- gene polymorphism with the pathogenetic mechanisms of placental dysfunction development

The role of the calcitriol / vitamin D receptor (VD) endocrine system and the pleiotropic effects of this system in the pathogenetic mechanisms of various diseases development, in particular complications of pregnancy, has attracted researches’ increasing attention in recent years. The aim of the work: to compare the VD-status and frequency of occurrence of polymorphism of the VDR gene (BsmI (A> G, rs1544410) in patients with a physiological course of the gestation process and in patients with placental dysfunction (PD). Materials and methods. 56 pregnant women with PD (the main group) and 40 patients with a physiological pregnancy (control group) were examined. VD status was determined by ELISA at level 25 (OH) D in serum, the frequency of BsmI polymorphism of the VDR gene (rs1544410) by polymerase chain reaction (PCR). Results. The average index of VD (31.40 ± 8.6) ng / ml in patients with PD is significantly lower than in patients with physiological pregnancy (43.54 ± 11.20) ng / ml, (p ≤ 0.05 ). In patients with PD, homozygous carrier for the A-allele was found in 12% of cases, in healthy pregnant women - in 16.7%, (р ≥ 0.05), for the G-allele - in 20% and 47.20%, (р ≤ 0.01) cases, respectively to groups. Heterozygous combination of A / G alleles was noted in 68% of patients with PD and in 36.10% of the control group patients. In pregnant women with BsmI polymorphism of calcitriol gene (genotype A / G) PD was 3.7 times more frequent (68% vs 36.10% : RR = 2.1, CI 1.0-6.6, OR = 3.7, CI 1.1-13.1). Conclusions. Vitamin D insufficiency or deficiency can be one of the reasons of PD formation. In carriers of BsmIgene’s polymorphism encoding VD receptor with genotype A / G, the course of pregnancy is complicated by placental dysfunction 3.7 times more often than in women without this polymorphism