Demir yükü artmış karaciğerde i̇nce i̇ğne aspirasyon sitolojisi (IIAS) ve tru-cut biyopsisi: deneysel bir çalışma

Objective: Fine needle aspiration cytology (FNAC) is safer, simpler, faster and cost effective, compared to tru-cut biopsy, and patient compliance is generally better. There is not much data on the use of FNAC, which is used in many organs and lesions, in parenchymal diseases in the liver. Therefore, in this study, the benefit of FNAC in showing iron deposition and injury of liver is evaluated. Methods: There were 2 control groups in the study. At the end of the study, FNAC and tru-cut biopsy samples were taken from these groups, where no administration was performed. FNAC and tru-cut biopsy procedures were also performed to the study groups after the iron overload was created by intraperitoneal iron dextran (88 mg/kg) administration for 28 days. Hepatocyte injury, inflammation and iron deposition were evaluated histopathologically in the preparations. The sensitivity, specificity, positive and negative predictivity and diagnostic accuracy of both methods were calculated based on the hepatectomy material. Results: In hepatocyte injury; sensitivity (60%), diagnostic accuracy (66.7%) and negative predictivity (50%) were found to be significantly higher in tru-cut group. It was observed that the methods were not superior to each other in the evaluation of inflammation, and FNAC almost approached tru-cut biopsy rates (100%) with a sensitivity of 83.3% and diagnostic accuracy in iron deposition. Conclusion: In iron-induced liver damage, the gold standard should be tissue examination, so tru-cut biopsy must be the first choice. However, based on the present study results, it is suggested that FNAC may be preferred over more invasive options in cases where tru-cut biopsy can not be used in terms of susceptibility to bleeding, situations where surgical accessibility is not available, patient orientation and cooperation is poor, or in presence of different accompanying diseases

The effect of ferula communis l. on body-relative organ weight, serum and tissue oxidative status, biochemical and pathological changes in rats exposed to continuous light

The goal of this study was to investigate the effects of Ferula communis L. on growth performance, relative organ weights, tissue and serum oxidative status, and biochemical and histopathological changes in rats exposed to continuous light. Rats exposed to continuous light for 10 days were given F. communis orally. Daily body weight was recorded, and rats were euthanized by cervical dislocation. Blood was collected to determine relative organ weights, followed by immediate biochemical and histopathological analysis of the organs. Serum and tissue oxidative status were measured. Continuous light exposure in rats resulted in weight loss, decreased ovary, uterus, and kidney weights, diminished total antioxidant status (TAS) along with increased cortisol, total oxidant status, and oxidative stress index. However, F. communis treatment reduced serum cortisol concentration and alleviated oxidative stress by increasing TAS even under prolonged light exposure conditions. Neither continuous light exposure nor F. communis treatment caused significant changes in malondialdehyde and glutathione in organ tissues. Although continuous light caused an increase in the number of cystic follicles, F. communis treatment did not seem to have a positive effect on cystic follicle formation. In conclusion, continuous light exposure stressed the rats and increased cortisol, as well as stimulated oxidative stress and cystic follicle formation. F. communis treatment can help alleviate the harmful effects of constant light exposure

Effects of Ozone and L-Carnitine on Kidney MDA, GSH, and GSHPx Levels in Acetaminophen Toxicity

This study aimed to determine the therapeutic effects of medical ozone and L-carnitine therapy on acetaminophen (APAP)-induced kidney damage by evaluating malondialdehyde (MDA), glutathione (GSH), and GSHPx levels. In this study, 56 rats were randomized into 8 groups with 7 rats in each group. Kidney injury was induced by the administration of a single dose N-acetyl-p-aminophenol (1 g/kg) orally.Therapeutic ozone (0.7 mg/kg) and L-carnitine (500 mg/kg) were administered intraperitoneally. After the therapy, the rat kidneys were homogenized, and the tissue MDA, GSH, and GSHPx levels were measured. Compared to the control groups, there were higher MDA levels in the kidney tissues only in the "APAP," "APAP + Ozone," and "APAP + Ozone + L-carnitine" groups (P<0.001). Besides, the decrease in the GSH and GSHPx levels of the kidney tissues in the study groups were significant compared to the control groups, and the highest decreases were observed in the "APAP" "APAP + Ozone" and "APAP + Ozone + L-carnitine" groups (P<0.001). Findings obtained from this study revealed that acetaminophen toxicity caused oxidative damage in the examined kidney tissues, and L-carnitine and/ or ozone applications for protective purposes decreased MDA levels, a product of lipid peroxidation, and increased tissue GSH levels thru GSHPx antioxidant enzyme activity. In this context, the most important protective effect was observed in the group where L-carnitine and ozone were administrated together

Selenium or ozone: Effects on liver injury caused by experimental iron overload

PubMed: 33038377Aims: Iron is an important metal ion as a biocatalyst on the other hand iron overload causes various diseases. Iron overload can result in fibrosis and hepatocellular carcinoma with various pathophysiological mechanisms, including oxidative damage in the liver. Therefore; in this study the effects of ozone and selenium -whose antioxidant properties are known- were evaluated in liver injury induced by iron overload. Materials and methods: Iron overload model was provided by intraperitoneal administration of 88 mg/kg iron dextrate for 4 weeks. After iron dextran administration, ozone and selenium administrations were made for 3 weeks. From the obtained blood and tissue samples total oxidant status (TOS) and total antioxidant status (TAS) were determined and histopathological examination was performed in liver tissue samples. Key findings: In rats with iron overload, the lowest mean serum TOS was observed in the selenium administration group. The highest tissue TOS means and the lowest tissue TAS means were determined in the group in which ozone and selenium were administrated together. When histopathological data were evaluated, the presence of increased apoptosis in the ozone group compared to the iron group (p = 0.019) and selenium group (p = 0.019) was noted. Similarly, increased periportal inflammation (p = 0.001) and fibrosis (p = 0.005) were observed in the ozone group compared to the selenium group. Significance: In iron-induced liver damage, ozone was thought to be effective by decreasing ROS, but contrary to expectations, it was observed that it may negatively affect the picture by showing synergistic effect. However, the effects of selenium on both serum and tissue levels are promising. © 2020 Elsevier Inc

Efficacy of Ozone and Selenium Therapy for Alcoholic Liver Injury: An Experimental Model

adali, yasemen/0000-0002-8004-7364WOS: 000465969100014PubMed: 31028195Background/Aim: Alcoholic liver disease is an important health problem which is reversible during early stages of liver damage, but becomes permanent with time. Nowadays, many studies focus on various agents that prevent, reduce or slow the progression of the toxic effects of alcohol. in our study, we investigated the efficiency of ozone and selenium against oxidative damage in a model of alcohol-induced liver damage. Materials and Methods: Forty-eight female Wistar Albino rats between 4 and 6 months of age and weighing 190-250 g were included in the study and were used as models of alcohol liver damage. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum and tissue total oxidant levels, serum and tissue total antioxidant levels, and the histopathological evaluation of the liver were performed in 8 groups. Results: in the statistical analysis, it was observed that ozone and/or selenium therapies decreased the AST levels. Total oxidant and antioxidant serum levels were found to vary in serum and tissue. Ozone and/or selenium therapies decreased liver damage, according to histopathological findings. Conclusion: Through ozone and/or selenium therapies, less damage was observed histopathologically compared to the alcohol group. It is thought that the results of our study can be used in individual treatments following confirmation of liver damage in alcoholic patients.Kafkas UniversityKafkas University [2016-TS 39]This study was funded by Kafkas University (2016-TS 39). All applicable international, national, and/or institutional guidelines for the care and use of animals were followed

Comparison of tru-cut biopsy and fine-needle aspiration cytology in an experimental alcoholic liver disease model

MAKAV, MUSTAFA/0000-0003-1879-8180; Adali, Yasemen/0000-0002-8004-7364; EROGLU, Huseyin Avni/0000-0002-1040-3255; Gok, Mustafa/0000-0001-7021-0984; KARAYOL, SUNAY SIBEL/0000-0002-2696-3580WOS: 000568954700005PubMed: 32935794INTRODUCTION: Liver biopsies such as tru-cut (sharp needle) and fine-needle aspiration cytology (FNAC) are the most commonly preferred techniques to detect the grade and stage of certain liver diseases. in this study, we aimed to compare the efficiency of USG-guided tru-cut biopsy and fine-needle aspiration cytology in an experimental alcoholic liver disease model. METHODS: Thirty-six female Wistar albino rats, 4-6 months old, and weighing from 190 to 250 g, were used in this study. the animals were randomly divided into six equal groups: GI (control), G2 (tru-cut control), G3 (FNAC control), G4 (Alcoholic liver disease model), G5 (Alcoholic liver disease model + FNAC), and G6 (Alcoholic liver disease model + tru-cut biopsy). After a histopathological evaluation by light microscopy, the sensitivity, specificity, positive and negative predictive values of FNAC and tru-cut biopsy for the diagnosis of liver lesions were calculated. RESULTS: No pathology was detected in GI except for mild congestion. on the other hand, hepatocyte damage, periportal inflammation, congestion, and fatty changes were detected in all liver tissues of the alcoholic liver disease groups. the sensitivity of hepatocyte damage, inflammation, congestion, and fatty change parameters for FNAC were 33.3%, 80%, 0%, and 0%, respectively, while the sensitivity of the same variables for tru-cut were 66.7%, 40%, 100%, and 20%, respectively. DISCUSSION: Both techniques were superior in some aspects. FNAC can be an attractive alternative to tru-cut biopsy and applied in routine practice in the diagnosis of non-tumoral liver diseases.Kafkas UniversityKafkas University [2016-TS-56]This study is funded by Kafkas University (Project number: 2016-TS-56)

The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats

Objective: To investigate the effects of quercetin (Q) and rutin on 5-fluorouracil (5-FU)-induced hepatotoxicity. Methods: The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU (50 mg/kg) in the 18th day. The group rutin 100 was rutin (100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU (50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin (100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized rats. Results: The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU groups. Conclusions: In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity

Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats

The anti-inflammatory and anti-apoptotic effects of molecular hydrogen, delivered as hydrogen-rich saline (HRS), on spinal cord injury was investigated. Four-month-old male Sprague Dawley rats (n = 24) were classified into four groups: (1) control—laminectomy only at T7-T10; (2) spinal injury—dura left intact, Tator and Rivlin clip compression model applied to the spinal cord for 1 min, no treatment given; (3) HRS group—applied intraperitoneally (i.p.) for seven days; and (4) spinal injury—HRS administered i.p. for seven days after laminectomy at T7–T10 level, leaving the dura intact and applying the Tator and Rivlin clip compression model to the spinal cord for 1 min. Levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured in blood taken at day seven from all groups, and hematoxylin–eosin (H & E) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) were used to stain the tissue samples. IL-6 and TNF-α levels were significantly lower in the group treated with HRS following the spinal cord injury compared to the group whose spinal cord was damaged. A decrease in apoptosis was also observed. The anti-inflammatory and anti-apoptotic effect of IL-6 may be a clinically useful adjuvant therapy after spinal cord injury

The Investigation of the Effect of Ozone Therapy on Gout in Experimental Rat Models

The effect of ozone treatment in experimental rat gout arthritis was investigated. Twenty adult albino male rats were divided into 4 groups: Group 1 (Control) was followed without any medication, Group 2 (Gout control) was injected with a single dose of 0.2 mL (10 mg) monosodium urate (MSU) intraarticulary to 1st metatarsophalangeal joint to create experimental Gouty arthritis. Group 3 (Gout + Ozone) was injected intraarticulary to 1st metatarsophalangeal joint with a single dose of 0.2 mL (10 mg) MSU to generate experimental gout, followed by ozone therapy to the rats a single dose of 1 mL (10 mu g/mL) intraperitoneally daily for 3 weeks. The Group 4 (Ozone control) ozone therapy was given a single dose of 1 mL (10 mu g/mL) intraperitoneally daily for 3 weeks without gout formation. The results were recorded and compared statistically between the groups. In conclusion, the low level of inflammation in the third group (Gout + Ozone) was considered statistically significant as compared to the second group. With Ozone treatment the gout joint has been shown to have reduced acute inflammation, it has not been affected by the chronic proliferation process