72 research outputs found
Influence of flow velocity, river size, a dam, and an urbanized area on biodiversity of lowland rivers
Biodiversity of aquatic organisms is formed under the influence of not only natural, but also anthropogenic factors. In this work, the influence of the flow velocity, river size, flow regulation and urbanization on various groups of aquatic organisms was studied in several lowland rivers. The study was conducted in 2013 on six tributaries of the Upper Sukhona River. Five sampling sites were in different parts of the Vologda River and five sites on small rivers, Losta, Lukhta, Komya, Chernyj Shingar, and Belyj Shingar (one site per river). Phytoplankton, zooplankton and zoobenthos were sampled six times, from April to October, and macrophytes were studied in August. In total, 469 species of aquatic organisms were found in the tributaries of the Upper Sukhona River, belonging to the following phyla: Cyanophyta (5 species), Chrysophyta (8), Bacillariophyta (62), Xanthophyta (1), Cryptophyta (10), Dinophyta (4), Euglenophyta (12), Chlorophyta (17), Streptophyta (1), Bryophyta (2), Marchantiophyta (3), Equisetophyta (1), Magnoliophyta (63), Rotifera (22), Cnidaria (1), Platyhelmintes (1), Annelida (29), Mollusca (33), Arthropoda (194). The maximum number of species was found in the Vologda River, the largest of all the tributaries. The number of zoobenthos species was similar at different sites in the Vologda River and in the small rivers. The number of species of other groups of aquatic organisms in the small rivers was lower than those registered in the Vologda River. The greatest number of macrophyte and zoobenthos species was recorded in the Upper Vologda River and Belyj Shingar River, where the flow is strong all the year round. The greatest number of phyto- and zooplankton species was found at the extra-city sites where current is almost absent. In the dam backwater, species richness was higher than that registered downstream of the dam. At the same time, the species richness of macrophytes and zoobenthos in the dam backwater was lower. The smallest number of species was found in the Vologda River, downstream of the city of Vologda. Decreases in the species richness and Shannonβs biodiversity index were witnessed in the Vologda River city site and in the small rivers, as they get closer to the city. Cluster analyses performed for the studied groups of aquatic organisms showed dissimilar results; however, the studied sites on the Vologda River having the highest anthropogenic load formed a cluster. Aquatic organisms of the Upper Sukhona tributaries experience both natural (flow velocity and size of the watercourse) and anthropogenic factors (proximity to the city and flow regulation)
Π‘ΠΈΠ½Π΄ΡΠΎΠΌ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ
The aim of the study was to obtain new biochemical data on the pathogenesis of respiratory syncytial viral infection (RSVI) in children.Object and methods: 60 children aged 1 month to 5 years, treated in the clinic of Pediatric Research and Clinical Center for Infectious Diseases, from which in 50 patients RNA RSV was isolated in smears from the oropharynx. The comparison group consisted of 10 children who failed to verify RSVI by laboratory methods. All children at admission and before discharge from the hospital (after-7-9 days) underwent a clinical blood test a Sysmex XP-300 hematology analyzer (Japan). Alpha-1-antitrypsin and alpha-2-macroglobulin were determined in blood serum by quantitative immunoturbidimetry on a biochemical analyzer CLIMA-15 (Spain) using Sentinel test systems (Italy). Determination of the amount of total protein, albumin and C-reactive protein in serum was carried out on an automatic analyzer Taurus (Instrumentation Laboratory, Italy) using reagents of the company Β«Vector-bestΒ» (Russia). The study of protein fractions in blood serum was carried out by capillary electrophoresis on the device Minicap company Sebia (France) with the help of test systems Β«Minicap Protein(e) 6Β» of the same manufacturer. The levels of cytokines (IL-6, IL-10) in serum were determined by ELISA on ELISA analyzer Β«INFINITIΒ» (TECAN, Austria) using reagents firm Β«Vector-bestΒ» (Russia).Results: RSVI occurs with lesions of the lower respiratory tract in 42% of cases, with the development of complications in 44% of sick children. The study revealed a prolonged increase in serum alpha-2 fraction of globulins, immunoregulatory cytokines with pro-inflammatory (IL-6) and anti-inflammatory (IL-10) action and, which may indicate the presence of subacute inflammatory process associated with the persistence of RS-virus. Lower levels of gamma-globulin fraction, including the main specific and nonspecific immunoglobulins, in children with PCR-proven RSVI, both in the acute period and in the period of convalescence, probably can cause repeated RSV-diseases, as well as an increase in the risk of atopic diseases.Conclusion. The long-term increase in the level of subacute inflammation markers, established in the course of the study, even against the relieve of clinical picture of the disease, makes the question of developing an etiopathogenetic treatment of respiratory syncytial viral infection with the use of drugs with antiviral and anti-inflammatory action relevant.Π¦Π΅Π»Ρ: ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ Π½ΠΎΠ²ΡΡ
ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΎ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·Π΅ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: 60 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 1 ΠΌΠ΅ΡΡΡΠ° Π΄ΠΎ 5 Π»Π΅Ρ, ΠΏΠΎΠ»ΡΡΠ°Π²ΡΠΈΡ
Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π² ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ ΠΠ΅ΡΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΡΠ½ΠΎ-ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅Π½ΡΡΠ° ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ
Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ, ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
Ρ 50 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ Π² ΠΌΠ°Π·ΠΊΠ°Ρ
ΠΈΠ· ΡΠΎΡΠΎΠ³Π»ΠΎΡΠΊΠΈ Π²ΡΠ΄Π΅Π»Π΅Π½Π° Π ΠΠ Π Π‘Π. ΠΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ 10 Π΄Π΅ΡΠ΅ΠΉ, Ρ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½Π°Ρ Π²ΠΈΡΡΡΠ½Π°Ρ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ Π½Π΅ Π²Π΅ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π° Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΡΠ΅ΠΌ Π΄Π΅ΡΡΠΌ ΠΏΡΠΈ ΠΏΠΎΡΡΡΠΏΠ»Π΅Π½ΠΈΠΈ ΠΈ ΠΏΠ΅ΡΠ΅Π΄ Π²ΡΠΏΠΈΡΠΊΠΎΠΉ ΠΈΠ· ΡΡΠ°ΡΠΈΠΎΠ½Π°ΡΠ° ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΡΠΎΠ²ΠΈ Π½Π° Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Sysmex XP-300 (Π―ΠΏΠΎΠ½ΠΈΡ). Π ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΡΡΡΠ±ΠΈΠ΄ΠΈΠΌΠ΅ΡΡΠΈΠΈ Π½Π° Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅Β CLIMA-15 (ΠΡΠΏΠ°Π½ΠΈΡ) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌ ΡΠΈΡΠΌΡ Sentinel (ΠΡΠ°Π»ΠΈΡ) ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π°Π»ΡΡΠ°-1Π°Π½ΡΠΈΡΡΠΈΠΏΡΠΈΠ½ ΠΈ Π°Π»ΡΡΠ°-2-ΠΌΠ°ΠΊΡΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΎΠ±ΡΠ΅Π³ΠΎ Π±Π΅Π»ΠΊΠ°, Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π° ΠΈ Π‘-ΡΠ΅Π°ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Taurus (Instrumentation Laboratory, ΠΡΠ°Π»ΠΈΡ) Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΡΠΈΡΠΌΡ Β«ΠΠ΅ΠΊΡΠΎΡ-ΠΠ΅ΡΡΒ» (Π ΠΎΡΡΠΈΡ). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΡΡΠ°ΠΊΡΠΈΠΉ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·Π° Π½Π° ΠΏΡΠΈΠ±ΠΎΡΠ΅ Minicap ΡΠΈΡΠΌΡ Sebia (Π€ΡΠ°Π½ΡΠΈΡ) Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠ΅ΡΡ-ΡΠΈΡΡΠ΅ΠΌ Β«Minicap Protein(Π΅) 6Β» ΡΠΎΠΉ ΠΆΠ΅ ΡΠΈΡΠΌΡ-ΠΈΠ·Π³ΠΎΡΠΎΠ²ΠΈΡΠ΅Π»Ρ. Π£ΡΠΎΠ²Π΅Π½Ρ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (ΠΠ-6, ΠΠ-10) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠ²Π΅ΡΠ΄ΠΎΡΠ°Π·Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π½Π° ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Β«INFINITIΒ» (TECAN, ΠΠ²ΡΡΡΠΈΡ) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ΅Π°Π³Π΅Π½ΡΠΎΠ² ΡΠΈΡΠΌΡ Β«ΠΠ΅ΠΊΡΠΎΡ-ΠΠ΅ΡΡΒ» (Π ΠΎΡΡΠΈΡ). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½Π°Ρ Π²ΠΈΡΡΡΠ½Π°Ρ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡ ΠΏΡΠΎΡΠ΅ΠΊΠ°Π΅Ρ Ρ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ Π½ΠΈΠΆΠ½ΠΈΡ
Π΄ΡΡ
Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΡΠ΅ΠΉ Π² 42% ΡΠ»ΡΡΠ°Π΅Π², Ρ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ β Ρ 44% Π±ΠΎΠ»ΡΠ½ΡΡ
Π΄Π΅ΡΠ΅ΠΉ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΏΡΠΎΠ»ΠΎΠ½Π³ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π°Π»ΡΡΠ°-2 ΡΡΠ°ΠΊΡΠΈΠΈ Π³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ², ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ², ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ (ΠΠ-6) ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ (ΠΠ-10) Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ, ΡΡΠΎ ΠΌΠΎΠΆΠ΅Ρ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΎ Π½Π°Π»ΠΈΡΠΈΠΈΒ ΠΏΠΎΠ΄ΠΎΡΡΡΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Ρ ΠΏΠ΅ΡΡΠΈΡΡΠ΅Π½ΡΠΈΠ΅ΠΉ Π Π‘-Π²ΠΈΡΡΡΠ°. ΠΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ Π³Π°ΠΌΠΌΠ°-Π³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ²ΠΎΠΉ ΡΡΠ°ΠΊΡΠΈΠΈ, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ΅ΠΉ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ Π½Π΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½Ρ, Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π΄ΠΎΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ¦Π ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ, ΠΊΠ°ΠΊ Π² ΠΎΡΡΡΠΎΠΌ ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅, ΡΠ°ΠΊ ΠΈ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄Π΅ ΡΠ΅ΠΊΠΎΠ½Π²Π°Π»Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°ΡΡ ΠΏΠΎΠ²ΡΠΎΡΠ½ΡΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ Π°ΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π½ΠΎΠ΅ Π² Ρ
ΠΎΠ΄Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΏΠΎΠ΄ΠΎΡΡΡΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π΄Π°ΠΆΠ΅ Π½Π° ΡΠΎΠ½Π΅ ΠΊΡΠΏΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½Ρ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ Π΄Π΅Π»Π°Π΅Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌ Π²ΠΎΠΏΡΠΎΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ ΡΡΠΈΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎ-ΡΠΈΠ½ΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Ρ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΈΡΡΡΠ½ΡΠΌ ΠΈ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ.
Identification of Pax6-Dependent Gene Regulatory Networks in the Mouse Lens
Lineage-specific DNA-binding transcription factors regulate development by activating and repressing particular set of genes required for the acquisition of a specific cell type. Pax6 is a paired domain and homeodomain-containing transcription factor essential for development of central nervous, olfactory and visual systems, as well as endocrine pancreas. Haploinsufficiency of Pax6 results in perturbed lens development and homeostasis. Loss-of-function of Pax6 is incompatible with lens lineage formation and results in abnormal telencephalic development. Using DNA microarrays, we have identified 559 genes expressed differentially between 1-day old mouse Pax6 heterozygous and wild type lenses. Of these, 178 (31.8%) were similarly increased and decreased in Pax6 homozygous embryonic telencephalon [Holm PC, Mader MT, Haubst N, Wizenmann A, Sigvardsson M, GΓΆtz M (2007) Loss- and gain-of-function analyses reveals targets of Pax6 in the developing mouse telencephalon. Mol Cell Neurosci 34: 99β119]. In contrast, 381 (68.2%) genes were differently regulated between the lens and embryonic telencephalon. Differential expression of nine genes implicated in lens development and homeostasis: Cspg2, Igfbp5, Mab21l2, Nrf2f, Olfm3, Spag5, Spock1, Spon1 and Tgfb2, was confirmed by quantitative RT-PCR, with five of these genes: Cspg2, Mab21l2, Olfm3, Spag5 and Tgfb2, identified as candidate direct Pax6 target genes by quantitative chromatin immunoprecipitation (qChIP). In Mab21l2 and Tgfb2 promoter regions, twelve putative individual Pax6-binding sites were tested by electrophoretic mobility shift assays (EMSAs) with recombinant Pax6 proteins. This led to the identification of two and three sites in the respective Mab21l2 and Tgfb2 promoter regions identified by qChIPs. Collectively, the present studies represent an integrative genome-wide approach to identify downstream networks controlled by Pax6 that control mouse lens and forebrain development
Local Suppression of T Cell Responses by Arginase-Induced L-Arginine Depletion in Nonhealing Leishmaniasis
The balance between T helper (Th) 1 and Th2 cell responses is a major determinant of the outcome of experimental leishmaniasis, but polarized Th1 or Th2 responses are not sufficient to account for healing or nonhealing. Here we show that high arginase activity, a hallmark of nonhealing disease, is primarily expressed locally at the site of pathology. The high arginase activity causes local depletion of L-arginine, which impairs the capacity of T cells in the lesion to proliferate and to produce interferon-Ξ³, while T cells in the local draining lymph nodes respond normally. Healing, induced by chemotherapy, resulted in control of arginase activity and reversal of local immunosuppression. Moreover, competitive inhibition of arginase as well as supplementation with L-arginine restored T cell effector functions and reduced pathology and parasite growth at the site of lesions. These results demonstrate that in nonhealing leishmaniasis, arginase-induced L-arginine depletion results in impaired T cell responses. Our results identify a novel mechanism in leishmaniasis that contributes to the failure to heal persistent lesions and suggest new approaches to therapy
Biodiversity of macrophyte communities and associated aquatic organisms in lakes of the Vologda Region (north-western Russia)
This paper provides current data on the biodiversity of boreal lakes of the Vologda Region (north-western Russia), including macrophytes (vascular plants and macroscopic algae) and macrophyte inhabitants (invertebrates and microalgae). The raw data, given in two datasets (Sampling event dataset and an Occurrence dataset) and presented in the form of GBIF-mediated data, were collected from 139 lakes (macrophytes between 2005 and 2021, macrophyte inhabitants between 2014 and 2020). The dataset contains materials on the diversity of vascular plants (Tracheophyta, 3225 occurrences; Bryophyta, 155; Marchantiophyta, 16), macro- and microalgae (Ochrophyta, 546 occurrences; Chlorophyta, 193; Charophyta, 153; Cyanobacteria, 139; Cryptophyta, 86; Myzozoa, 33; Euglenozoa, 27; Rhodophyta, 8; Bigyra, 1) and aquatic invertebrates (Arthropoda, 1408 occurrences; Annelida, 487; Mollusca, 263; Platyhelminthes, 36; Cnidaria, 11). This paper summarises previously unpublished materials in a standardised form.The paper summarises the data collected during the long-term phytodiversity studies in a series of lakes of different types (Vologda Region, north-western Russia). Data on algae and invertebrates diversity were obtained in 60 different plant communities of aquatic, semi-aquatic and coastal plants or their combinations. A total of 6787 occurrences were included in the dataset, published in the global biodiversity database (GBIF) for the first time. According to the GBIF taxonomic backbone, the dataset comprised 837 taxa, including 711 lower-rank taxa (species, subspecies, varieties, forms). New records of 47 species rare and protected in the Vologda Region are given: 43 species of plants, three species of animals and one species of Cyanobacteria
EFFECT OF FUCOIDAN ON THE LEVEL AND DYNAMIC OF LIPID METABOLISM PARAMETERS IN P-407 INDUCED DYSLIPIDEMIA
Purpose: to study the effect of fucoidan (sulfated polysaccharide from brown algae) on the dynamics of lipid metabolism in the mice model of dyslipidemia induced poloxamer P-407. Materials and methods. We used fucoidan, extracted from brown algae Fucus evanescens with a molecular weight 160 kDa. Experimental studies were conducted on noninbred white mice. The model of dyslipidemia and atherosclerosis in animals was induced by intraperitoneal injection of poloxamer 407 (P-407). Results. We revealed the ability of per os administration of fucoidan to normalize the basic parameters of lipid metabolism in mice with dyslipidemia (serum levels of triglyceride, high-density lipoprotein cholesterol and very low-density lipoprotein cholesterol). Conclusion. Revealed experimental results allow to consider the fucoidan as the basis for the development of new biological products with lipid corrective action and to recommend it for further study in experimental and clinical trials
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