Potential of Iranian wild barley (Hordeum vulgare ssp. spontaneum) in breeding for drought tolerance

The vast genetic resources of wild barley (Hordeum vulgare ssp. spontaneum, hereafter WB) may hold unique assets for improving barley (H. vulgare ssp. vulgare) cultivars for drought stress. To evaluate genetic potential and characterization of variation among a diverse collection of barley and WB genotypes, mostly originated from Iran, a field experiment was performed under three moisture environments (control, mild and intense drought stress) during two years (2012–2014). Considerable variation was observed among the wild and cultivated genotypes for drought tolerance and agronomic traits. Principal component analysis (PCA) grouped genotypes studied into three groups (WB, two-row barley and sixrow barley groups). However, Iranian and foreign WB genotypes were not completely separated, showing a high variation within both gene pools. The high significance of genotype by environment interaction, confirms importance of using accurate target environments for drought stress breeding. A number of WB genotypes with the highest values of the number of tillers, number of seed per spike, seed weight, grain yield and yield stability index under stressed environments were identified as superior genotypes. Most of these genotypes originate from Iran, highlighting the importance of this germplasm in barley breeding

Industrial waste management in Shokuhieh industrial zone of Qom province in 2013

Background and aims: Nowadays, the rapid growth of industries and production of various products has led to an increase in hazardous industrial solid waste. The aim of this study was to examine how to manage industrial solid waste in industrial zone of Shokuhiyeh in Qom province in 2013. Methods: This research was a descriptive, cross-sectional one. The data were gathered by first referring to industrial units and the completion of Iranian Environmental Organization Questionnaire and then analyzing the gathered data. This questionnaire included 24 items covering the quantity, quality, and management of industrial solid waste. In this study, 41 industrial units with personnel of more than 50 were examined. Results: The category of" chemical and metal industries" produced the highest solid waste and specific solid waste. The elements making up the produced solid waste were plastic 38% and glass 27.5%, respectively. Among the storage methods, the temporary solid waste storage in baskets 36% and open-doors method 32% were the most common ones. 41% of industrial units disposed of solid waste monthly, 31.7% weekly, 24.4% daily and 2.4% in an inconsistent manner. 58% of produced solid waste was recycled, 35% was landfilled, 4.6% was burned up, and 2.4% was disposed of in an unknown manner. Conclusion: The results of this study showed that the collection and storage of industrial solid waste in the industrial zone of Shokuhiyeh is not done in a suitable manner. Moreover, no educational program has yet been done about the management of solid waste in most of the industries. Therefore, it seems indispensable to establish a suitable system of solid waste management in order to organize the conditions of industrial solid waste produced in this zone

Effect of Acidic Biochars on Some Chemical Properties and Nutrient Availabilities of Calcareous Soils

Low organic matter content and alkaline pH of calcareous soils in arid and semi-arid regions are the main reasons for the low nutrient availabilities for plants in these soils. One way to improve the chemical properties and fertility of calcareous soils is the application of organic substances such as biochar produced from pyrolysis of organic wastes. However, biochars have an almost predominant alkaline pH, which exacerbates plant nutrient deficiencies in calcareous soils when used for a long time. Pyrolysis of some organic wastes under controlled temperature conditions can lead to the production of acidic biochar. The effect of acidic biochars on several chemical properties of two calcareous soils in Isfahan province was investigated in the present study. Treatments included two types of biochar (pine cone and rice husk), three levels of biochar addition (one, three, and six percent), two types of soil (a sandy loam (Tiran) and a clay loam (Lavark)), and two incubation periods (one and six months). The results showed that applying biochar could slightly decrease soil pH but raised soil electrical conductivity. In addition, the amount of organic carbon, total nitrogen, and available concentration of manganese in all treatments and the concentrations of available phosphorus, potassium, iron, zinc, and copper in the most of treatments showed a significant increase compared to the control. Amending soil with biochar at a 6% rate caused the most significant changes in the measured parameters in both soil types. In general, the results of this study indicated that acidic biochar produced from pine cones and rice husk can be used as a suitable conditioner to improve the chemical properties and fertility of calcareous soils

The Effects of Two Different Deficit Irrigation Managements on the Root Length of Maize

The response of root to water stress is one of the most important parameters for researchers. Study of growth and distribution of root under different irrigation managements helpsresearchersto a better understanding of soil water content, and the availability of water and nutrition in water stress condition. To investigate the effects of four levels of irrigation under two different deficit irrigation managements on the root length of maize, a study was conducted in 2009. Irrigation managements included fixed irrigation interval-variable irrigation depth (M1) and variable irrigation interval-fixed irrigation depth (M2). Maize plants were planted in 120 large 110-liter containers in a strip-plot design in a randomized complete block with three replications. Root data sampling was done after root washing in five growth stages. The results showed that the effect of irrigation levels on root length was significant (

The effect of CRISPR constructs microinjection on the expression of developmental genes in Rag1 knocked-out mice embryo

Despite all the advances in the production of transgenic mice, the production efficiency of these animal models is still low. Given that the expression of developmental genes has a critical role in growth and development of embryo, we determined the expression pattern of pluripotency, trophectoderm and imprinting genes in the Rag1 (recombination-activating gene 1) knocked-out blastocysts resulting from microinjection of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) constructs into the zygote cytoplasm of C57bl6 mice. Following microinjection, the embryos were cultured and the gene expression of developed blastocysts and natural blastocysts (Sham and control groups) were evaluated using real-time PCR. The agarose gel to confirm the deletion in the Rag1 gene in Rag1 knocked-out blastocyst. Our results showed that the expression of trophectoderm genes (-TEAD-4 and Cdx2), pluripotency genes (Nanog and Oct-4) and imprinting gene (H19) in the Rag1 knocked-out group was significantly lower compared with the embryos obtained from Natural fertilization. According to these findings, manipulation, embryo culture and microinjection of CRISPR constructs into the zygote cytoplasm of mice led to reduced expression of imprinting, pluripotency and trophectoderm genes. Therefore, the Rag1 knocked-out embryos produced by the CRISPR/Cas9 system are of low quality, which reduces the chances of live birth in these animals and may cause various abnormalities in fetuses. © 2021 The Authors Veterinary Medicine and Science Published by John Wiley & Sons Lt