Flux of Ca2+ ions in the synaptosomal mitochondrial membrane

In the present study, the flux of Ca2+ ions in the synaptosomal mitochondrial membrane isolated from the whole brain and hippocampus of chronically ovariectomized female rats was examined. Under basal conditions no significant difference was found. Addition of estradiol (0.5 nmol/l) in the preincubation mixture decreased significantly (25%) Na-dependent Ca2+ efflux in mitochondria from both sources which may be the way that it exerts its role in nerve cell homeostasis.Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 200

Izolacija, kultivizacija i karakterizacija ćelija poreklom iz tumora i tumorske margine pacijenata sa bazocelularnim karcinomom u in-vitro uslovima

renewal and regeneration and the epidermis harbors a special cell population responsible for the process of self-renewal and regeneration of the skin, so called epidermal stem cells that have the ability to proliferate, migrate, differentiate, and undergo programmed cell death. If imbalance between these processes occurs, malignant transformation of healthy stem cell into the cancerous stem cell may happen. It is also known that a specific population of tumor cells called cancer stem cells is responsible for the hemoresistance, metastasis, and recurrence. Studies have shown that skin basal cell carcinoma is caused by stem cells of hair follicle, hair infundibulum and interfollicular epidermis. Basocellular carcinoma (BCC) is the most common malignant tumor of the skin, but it rarely gives metastases It is characterized by invasive growth, infiltrating surrounding structures and leading to serious functional and aesthetic defects. In the literature, inadequate margins are indicated as one of the key factors responsible for tumor recurrences. Studies have shown that for the primary facial skin BCC of less than 20 mm a margin of 3 mm is recommended. The aim of this study was to identify and characterize the cell subpopulation with stem characteristics whithin BCC and its margins (3 mm close) by testing proliferative, clonogenic and migratory potential, by analyzing the expression of embryonic, mesenchymal and tumor markers, by analizing biochemical structure of cells, by examining the differentiation potential of generated cell lines and by testing the degree of chemo-resistance. The study included 13 patients with primary BCC of face skin of less than 20 mm. Following a detailed anamnesis and a signed informed consent, the surgical excision which is considered a gold standard in BCC therapy, was done. A portion of the tumor tissue, of close margin (3 mm from the macroscopic tumor limit), and of healthy skin included in the resecion (at least 5mm from the macroscopic tumor limit), were obtained from all the patients. The remaining of the tissue was subjected to histopathological analysis. For cell phenotype characterization, tests of proliferation, colony formation, scratch assay, and sphere formation were used...Homeostaza kože direktno zavisi od mehanizma kontinuiranog obnavljanja ćelijske populacije kože i njene sposobnosti regeneracije. U epidermisu je prisutna posebna populacija ćelija zadužena za proces samoobnavljanja kože, tzv. epidermalne matične ćelije koje imaju sposobnost proliferacije, migracije, diferencijacije i programirane ćelijske smrti. Ukoliko dođe do narušavanja ravnoteže između ovih procesa, može doći do maligne transformacije zdrave matične ćelije u kancersku matičnu ćeliju. Poznato je da za hemorezistenciju, metastaziranje i recidiviranje tumora odgovornost nosi posebna populaciju tumorskih ćelija koje se nazivaju kancerske matične ćelije. Istraživanja pokazuju da bazocelularni karcinom kože vodi poreklo od matičnih ćelija ispupčenja folikula dlake, infundibuluma dlake i interfolikularnog epidermisa. Bazocelularni karcinom (BCK) je najčešći maligni tumor kože, pa i ako retko metastazira karakteriše ga invazivni rast pri čemu infiltriše okolne strukture i dovodi do ozbiljnih funkcionalnih i estetskih defekata. U literaturi se kao jedan od ključnih faktora odgovornih za recidiviranje navodi neadekvatna širina margine resekcije tumora. Istraživanja su pokazala da je za primarne BCK na koži lica veličine do 20 mm preporučena širina margine 3 mm. Cilj ovog istraživanja bio je identifikacija i karakterizacija subpopulacije ćelija sa osobinama matičnosti izolovanih iz BCK-a i bliske margine od 3 mm, ispitivanjem proliferativnog, klonogenog i migratornog potencijala, analizom ekspresije embrionalnih, mezenhimskih i tumorskih markera, analizom biohemijske strukture ćelija, ispitivanjem sposobnosti diferencijacije u više ćelijskih linija kao i stepena otpornosti ćelija na dejstvo citostatika. U istraživanju je učestvovalo 13 pacijenata sa primarnim bazocelularnim karcinomom na koži lica veličine do 20 mm. Nakon detaljno uzete anamneze i potpisanog informisanog pristanka pristupalo se hirurškoj eksciziji tumorske promene, koja se smatra zlatnim standardom u terapiji BCK-a. Iz svih ekscidiranih uzorka za izolaciju ćelija uzet je deo tumorskog tkiva, tkiva bliske margine (udaljene 3 mm od makroskopske granice tumora) i deo zdrave kože obuhvaćen oblikom resekcije (udaljen od makroskopske granice tumora više od 5 mm), dok je preostali deo tkiva poslat na histopatološku analizu. Za karakterizaciju ćelija u kulturi korišćeni su testovi proliferacije, migracije, formiranja kolonija, sfera i ramanska spektroskopija. Markeri kancerskih matičnih ćelija (KMĆ) analizirani su ,,Real-Time PCR” metodom i protočnom citometrijom..

Isolation, cultivation and characterization of cells originating from tumor and tumor margin of patients with basal cell carcinoma in vitro

Homeostaza kože direktno zavisi od mehanizma kontinuiranog obnavljanja ćelijske populacije kože i njene sposobnosti regeneracije. U epidermisu je prisutna posebna populacija ćelija zadužena za proces samoobnavljanja kože, tzv. epidermalne matične ćelije koje imaju sposobnost proliferacije, migracije, diferencijacije i programirane ćelijske smrti. Ukoliko dođe do narušavanja ravnoteže između ovih procesa, može doći do maligne transformacije zdrave matične ćelije u kancersku matičnu ćeliju. Poznato je da za hemorezistenciju, metastaziranje i recidiviranje tumora odgovornost nosi posebna populaciju tumorskih ćelija koje se nazivaju kancerske matične ćelije. Istraživanja pokazuju da bazocelularni karcinom kože vodi poreklo od matičnih ćelija ispupčenja folikula dlake, infundibuluma dlake i interfolikularnog epidermisa. Bazocelularni karcinom (BCK) je najčešći maligni tumor kože, pa i ako retko metastazira karakteriše ga invazivni rast pri čemu infiltriše okolne strukture i dovodi do ozbiljnih funkcionalnih i estetskih defekata. U literaturi se kao jedan od ključnih faktora odgovornih za recidiviranje navodi neadekvatna širina margine resekcije tumora. Istraživanja su pokazala da je za primarne BCK na koži lica veličine do 20 mm preporučena širina margine 3 mm. Cilj ovog istraživanja bio je identifikacija i karakterizacija subpopulacije ćelija sa osobinama matičnosti izolovanih iz BCK-a i bliske margine od 3 mm, ispitivanjem proliferativnog, klonogenog i migratornog potencijala, analizom ekspresije embrionalnih, mezenhimskih i tumorskih markera, analizom biohemijske strukture ćelija, ispitivanjem sposobnosti diferencijacije u više ćelijskih linija kao i stepena otpornosti ćelija na dejstvo citostatika. U istraživanju je učestvovalo 13 pacijenata sa primarnim bazocelularnim karcinomom na koži lica veličine do 20 mm. Nakon detaljno uzete anamneze i potpisanog informisanog pristanka pristupalo se hirurškoj eksciziji tumorske promene, koja se smatra zlatnim standardom u terapiji BCK-a. Iz svih ekscidiranih uzorka za izolaciju ćelija uzet je deo tumorskog tkiva, tkiva bliske margine (udaljene 3 mm od makroskopske granice tumora) i deo zdrave kože obuhvaćen oblikom resekcije (udaljen od makroskopske granice tumora više od 5 mm), dok je preostali deo tkiva poslat na histopatološku analizu. Za karakterizaciju ćelija u kulturi korišćeni su testovi proliferacije, migracije, formiranja kolonija, sfera i ramanska spektroskopija. Markeri kancerskih matičnih ćelija (KMĆ) analizirani su ,,Real-Time PCR” metodom i protočnom citometrijom...renewal and regeneration and the epidermis harbors a special cell population responsible for the process of self-renewal and regeneration of the skin, so called epidermal stem cells that have the ability to proliferate, migrate, differentiate, and undergo programmed cell death. If imbalance between these processes occurs, malignant transformation of healthy stem cell into the cancerous stem cell may happen. It is also known that a specific population of tumor cells called cancer stem cells is responsible for the hemoresistance, metastasis, and recurrence. Studies have shown that skin basal cell carcinoma is caused by stem cells of hair follicle, hair infundibulum and interfollicular epidermis. Basocellular carcinoma (BCC) is the most common malignant tumor of the skin, but it rarely gives metastases It is characterized by invasive growth, infiltrating surrounding structures and leading to serious functional and aesthetic defects. In the literature, inadequate margins are indicated as one of the key factors responsible for tumor recurrences. Studies have shown that for the primary facial skin BCC of less than 20 mm a margin of 3 mm is recommended. The aim of this study was to identify and characterize the cell subpopulation with stem characteristics whithin BCC and its margins (3 mm close) by testing proliferative, clonogenic and migratory potential, by analyzing the expression of embryonic, mesenchymal and tumor markers, by analizing biochemical structure of cells, by examining the differentiation potential of generated cell lines and by testing the degree of chemo-resistance. The study included 13 patients with primary BCC of face skin of less than 20 mm. Following a detailed anamnesis and a signed informed consent, the surgical excision which is considered a gold standard in BCC therapy, was done. A portion of the tumor tissue, of close margin (3 mm from the macroscopic tumor limit), and of healthy skin included in the resecion (at least 5mm from the macroscopic tumor limit), were obtained from all the patients. The remaining of the tissue was subjected to histopathological analysis. For cell phenotype characterization, tests of proliferation, colony formation, scratch assay, and sphere formation were used..

The Inventory of Music Materials of the Cathedral Chapter in Hvar, 1646/1647

U Kaptolskome arhivu u gradu Hvaru sačuvan je rukopisni izvor iz 1646/47, u kojemu su popisane knjige u ondašnjem vlasništvu hvarskoga Kaptola. Među njima su i danas izgubljene muzikalije, no čijim je nominalnim tragom precizno dokumentirana kvantiteta i kvaliteta glazbene građe pristigle u hvarsku (i uopće neku dalmatinsku) sredinu tijekom prve polovice 17. stoljeća. Osim što svjedoči o prisutnosti recentne talijanske (ponajviše venecijanske ranobarokne) glazbene literature u hvarskoj sredini, inventar kaptolskih muzikalija otkriva i naslove četiriju do sada nepoznatih zbirki skladatelja T. Cecchinija, koje su u ovome radu pridružene otprije poznatim opusima u revidiranome popisu Cecchinijevih djela. S obzirom na značenje ovog izvora u kontekstu hrvatske glazbene historiografije i kulture (ranoga) baroka, žarišna je točka ovoga članka pregled glazbenog sadržaja kaptolskoga inventara, ponajprije uz pomoć tablice s transkripcijama naslova i dodatnim podacima o autorima i tiskanim izdanjima, za koja je moguće da su nekoć bila dijelom kaptolske zbirke.A manuscript kept in the Cathedral Chapter Archives in Hvar contains a list of books and music material owned by the Cathedral Chapter back in 1646/47. Although lost nowadays, recorded music titles are witness to the quantity and quality of music in the town of Hvar (and generally in Dalmatia) during the 1st half of the 17th century. This (music) inventory testifies not only to the presence of contemporary Italian (mostly Venetian early-Baroque) music literature on the island; it also reveals four titles of Tomaso Cecchini’s to date unknown opuses, joined here to the revised list of Cecchini’s works. Considering the importance of this source in the context of Croatian music historiography and (early) Baroque culture, a focal point of this article is an overview of the inventory’s musical content. It is presented here by way of a table containing transcripted titles and extra details on authors and printed editions, which were formerly a part of the Cathedral Chapter’s music collection

Repertoire for Klapa Singing Groups at the Dalmatian Song Evenings (Večeri dalmatinske pisme) Festival in Kaštela (1999-2008)

Od početka devedesetih godina 20. stoljeća klapsko pjevanje raste u pokret koji se širi izvan lokalnih i regionalnih granica klapske baze, a — uz osvajanje nagrade na Festivalu dalmatinskih klapa u Omišu — mjerilo — uspjeha klape postaje i njezina medijska eksponiranost, što je donekle utjecalo na nastanak I razvoj novog tipa klape, sklonog okretanju novim izvorima (najčešće popularnoj glazbi) te novom načinu prezentiranja i komuniciranja. Riječ je o modernoj klapi, popularnoj među najširom publikom, naklonjenoj suradnji s poppjevačima te posezanju za elementima popularnih žanrova kao što su rap, soul, rock, raggae. Tadašnje okolnosti u klapskome svijetu indirektno su utjecale na nastanak i razvoj Večeri dalmatinske pisme (VDP), festival osnovanog 1999. godine u Kaštel Kambelovcu, čiji je jedan segment u potpunosti posvećen klapama i njihovu čitanju postojećih domaćih (najčešće dalmatinskih) i stranih popularnih pjesama te izvođenju novih skladbi, pomalo netipičnih za kanonski klapski repertoar, dok u drugome segmentu novo ruho tradicijskih klapskih pjesama predstavljaju izvođači iz sfere popularne glazbe, najčešće jazza, rocka, bluesa, etna, zatim ča- i otočkog vala. U prvom desetljeću održavanja VDP-a na klapskim je večerima izvedeno otprilike tristo šezdeset skladbi i obradba, koje bi se okvirno mogle podijeliti na: obradbe tradicijskih napjeva (koje su prevladavale prvih dviju godina, kada sadržaj i nazivi pojedinih večeri, kao i podjela na klapski i neklapski segment VDP-a još nisu bili u potpunosti definirani), skladbe (izvedene mahom na Večeri novih skladbi), zatim obrade stranih (Klapske novitade) te domaćih pjesama uglavnom iz sfere popularne glazbe (Kaštelanski đir). Autorica je analizom nekolicine primjera iz navedenih skupina pokušala odgonetnuti neke od postupaka koje su autori primjenjivali u svojim skladbama i/ili obradbama, često po cijenu ili uz težnju odmaka od karakteristika tradicijskog klapskog repertaora.Since the beginning of the 1990s, klapa singing has grown into a movement that has crossed its local and regional borders. Along with winning awards at the Festival of Dalmatian Klapas Omiš (Festival dalmatinskih klapa Omiš), the success criteria for klapas also became their media exposure, which partly influenced the emergence of a new type — the modern klapa — which searches for new music sources and new ways of presentation and communication. Because of its collaboration with pop singers and reaching out for elements of popular music genres such as rap, soul, rock, and reggae, this type became popular amongthe widest audiences. Circumstances of that period indirectly influenced the founding and development of the Dalmatian Song Evenings (Večeri dalmatinske pisme), a festival founded in 1999 in Kaštel Kambelovac, Dalmatia. Its first section is dedicated to klapa performances of Croatian (mostly Dalmatian) and foreign popular songs arrangements, but also of new compositions, quite atypical for the canonic klapa repertoire. In the second section, popular music (jazz,rock, bluese, etno, ča- and island-wave) performers present new readings of traditional klapa songs. In the first decade of this festival, klapas performed about 360 compositions and arrangements, which could be roughly divided into: arrangements of traditional chants (which prevailed in the beginning, when the content and name of the festival events hadnot yet been defined), new compositions, performed at the New Compositions Evenings [Večeri novih skladbi], arrangements of foreign (performed at the Klapa Novelties event [Klapske novitade]) and domestic popular songs (performed at the Kaštela Style event [Kaštelanski đir]). Through the analysis of several examples, the author of this article has tried to reveal some of the new approaches found in modern klapa songs used by composers and arrangers, often with the price or tendency of neglecting the main characteristics of the traditional klapa repertoire

WHAT KIND OF MUSIC REPERTORY MIGHT HAVE BEEN PERFORMED TO THE AUDIENCE IN THE TOWN OF HVAR DURING THE 1ST HALF OF THE 17TH CENTURY?

U rukopisu iz 1646./47., pohranjenom u Kaptolskom arhivu u Hvaru, popisane su knjige i muzikalije u nekadašnjem vlasništvu Kaptola. Naslovi danas izgubljenih glazbenih zbirki zabilježeni su u osam sekcija s ukupno sedamdeset i tri jedinice te predstavljaju vrijedno svjedočanstvo o prisutnosti suvremene talijanske (ponajviše venecijanske ranobarokne) glazbene literature, čije je izvedbe mogla čuti publika u Hvaru do kraja prve polovice 17. stoljeća. Ovaj članak donosi osvrt na svaku od popisanih sekcija, a osnovne karakteristike kaptolskog glazbenog fonda prikazane su i u priloženim tablicama, s građom predstavljenom prema skladateljima, godinama i izdavačima prvotiska, te glazbenim vrstama, koje se odnose na pojedine jedinice u inventaru.Most of Croatian towns and villages are missing the archival sources which could demonstrate the quality and the quantity of music repertory in the first half of the 17th century. However, a manuscript kept in the Cathedral Chapter Archive in Hvar (on the island of Hvar) contains a list of books and music material owned by the Cathedral Chapter back in 1646/47, which could serve as strong and very rare evidence on art music practice in the 17th century Hvar, and generally in Dalmatia. Although lost nowadays, recorded music titles, listed in eight different sections, are witnessing the presence of contemporary Italian (mostly Venetian early-baroque) music literature once in the town. Each section of music inventory is discussed in this article, with addition of tables showing its content regarding composers, years and publishers of first editions, and music genres