Design of New Product for a Travel Agency

Diplomová práca sa zaoberá návrhom nového produktu pre cestovnú kanceláriu. Cieľom práce je navrhnúť podobu produktu pre cestovnú kanceláriu, ktorý bude vyhovovať potrebám a prianiam zákazníkov z Generácie Y. Zdrojom dát bol skupinový rozhovor a elektronické dopytovanie. Analýzou sa podarilo navrhnúť vhodné koncepty produktov. Na základe analýzy a zistených výsledkov boli cestovnej kancelárii Vítkovice Tours navrhnuté odporúčania.This thesis deals with designing a new product for a travel agency. The aim of the work is to design a form of travel agency product that will meet the needs and wishes of Generation Y customers. The source of data was a focus group and an electronic questionnaire survey. The analysis has succeeded in designing appropriate product concepts. On the basis of the analysis and the results obtained, there were recommendations suggested by the Vítkovice Tours travel agency.116 - Katedra marketingu a obchoduvýborn

Analýza nákupního chování Generace Y

Import 02/11/2016This thesis deals with the buying behavior of Generation Y. The aim of this work is to identify and describe the specifics and preferences of Generation Y on drugstore market. The source of data was an electronic questionnaire survey. Results of the research work should help to understand the needs and demands of Generation Y on the drugstore market.Bakalárska práca sa zaoberá nákupným správaním Generácie Y. Cieľom práce je zistiť a popísať špecifiká a preferencie Generácie Y pri nákupe drogistického tovaru. Zdrojom dát bolo elektronické dotazníkové šetrenie. Výsledky výskumu práce by mali pomôcť k pochopeniu potrieb a požiadaviek Generácie Y pri nákupe drogistického tovaru.116 - Katedra marketingu a obchoduvýborn

Synergistic effect of CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin on food intake regulation in lean mice

<p>Abstract</p> <p>Background</p> <p>CART (cocaine- and amphetamine-regulated transcript) peptide and cholecystokinin (CCK) are neuromodulators involved in feeding behavior. This study is based on previously found synergistic effect of leptin and CCK on food intake and our hypothesis on a co-operation of the CART peptide and CCK in food intake regulation and Fos activation in their common targets, the nucleus tractus solitarii of the brainstem (NTS), the paraventricular nucleus (PVN), and the dorsomedial nucleus (DMH) of the hypothalamus.</p> <p>Results</p> <p>In fasted C57BL/6 mice, the anorexigenic effect of CART(61-102) in the doses of 0.1 or 0.5 μg/mouse was significantly enhanced by low doses of CCK-8 of 0.4 or 4 μg/kg, while 1 mg/kg dose of CCK-A receptor antagonist devazepide blocked the effect of CART(61-102) on food intake. After simultaneous administration of 0.1 μg/mouse CART(61-102) and of 4 μg/kg of CCK-8, the number of Fos-positive neurons in NTS, PVN, and DMH was significantly higher than after administration of each particular peptide. Besides, CART(61-102) and CCK-8 showed an additive effect on inhibition of the locomotor activity of mice in an open field test.</p> <p>Conclusion</p> <p>The synergistic and long-lasting effect of the CART peptide and CCK on food intake and their additive effect on Fos immunoreactivity in their common targets suggest a co-operative action of CART peptide and CCK which could be related to synergistic effect of leptin on CCK satiety.</p

New pharmacological interventions influencing food intake focused on effects of CART (cocaine- and amphetamine- regulated transcript) peptide and prolactin-releasing peptide

The thesis was focused on characterization of biological activities of two recently discovered anorexigenic neuropeptides: CART (cocaine- and amphetamine- regulated transcript) peptide and prolactin-releasing peptide (PrRP). In order to find a pharmacophore of CART peptide, shorter fragments of CART(61- 102) peptide were tested for binding to PC12 cells and nhibition of food intake in fasted mice. The results showed that a compact structure of CART peptide containing three disulphide bridges is necessary for preservation of its full biological activity. In the second part of the thesis, synergistic and long-lasting effect of centrally administered peptide CART and peripherally administered cholecystokinin (CCK) is described. In fasted C57BL/6 mice, the anorexigenic effect of CART was enhanced by a subthreshold dose of CCK, while CCK1 receptor antagonist devazepide blocked the effect of CART peptide on food intake. In the third part of the thesis, food intake in fed lean and MSG (monosodium- glutamate treated) obese male mice with lesions in nucleus arcuatus (ARC) showed that anorexigenic action of CART peptide was preserved but satiety effect of CCK was completely lost and therefore, effective leptin signaling in ARC is necessary for satiety effect of CCK. Finally, the PrRP receptor was detected in..

Fluorescent fragments of oxytocin, vasopressin and dDAVP

Ornithin derivatives of neurohypophyseal hormone fragments 4-9 were synthesized and labeled by 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)

Buňky PC12 obsahují vazebná místa pro CART

Specific binding of 125I-CART(61-102) to the rat adrenal pheochromocytoma PC12 cell line, both intact cells and cell membranes is reported

New pharmacological interventions influencing food intake focused on effects of CART (cocaine- and amphetamine- regulated transcript) peptide and prolactin-releasing peptide

The thesis was focused on characterization of biological activities of two recently discovered anorexigenic neuropeptides: CART (cocaine- and amphetamine- regulated transcript) peptide and prolactin-releasing peptide (PrRP). In order to find a pharmacophore of CART peptide, shorter fragments of CART(61- 102) peptide were tested for binding to PC12 cells and nhibition of food intake in fasted mice. The results showed that a compact structure of CART peptide containing three disulphide bridges is necessary for preservation of its full biological activity. In the second part of the thesis, synergistic and long-lasting effect of centrally administered peptide CART and peripherally administered cholecystokinin (CCK) is described. In fasted C57BL/6 mice, the anorexigenic effect of CART was enhanced by a subthreshold dose of CCK, while CCK1 receptor antagonist devazepide blocked the effect of CART peptide on food intake. In the third part of the thesis, food intake in fed lean and MSG (monosodium- glutamate treated) obese male mice with lesions in nucleus arcuatus (ARC) showed that anorexigenic action of CART peptide was preserved but satiety effect of CCK was completely lost and therefore, effective leptin signaling in ARC is necessary for satiety effect of CCK. Finally, the PrRP receptor was detected in..

Specific binding of prolactin-releasing peptide analogues in pituitary cells

We report specific binding of 125I-PrRP31 to the following cell lines: GH3 cells (rat – somatotrophs), AtT20 cells (mouse – adenocorticotrophs) and RC-4B/C cells (rat- somatotrophs, lactrophs, adenocortitrophs and gonadotrophs). These results will serve as a basic knowledge for future structure-activity studie sof PrRP

Design and Synthesis of Highly Active Antimycobacterial Mutual Esters of 2-(2-Isonicotinoylhydrazineylidene)propanoic Acid

The combination of two active scaffolds into one molecule represents a proven approach in drug design to overcome microbial drug resistance. We designed and synthesized more lipophilic esters of 2-(2-isonicotinoylhydrazineylidene)propanoic acid, obtained from antitubercular drug isoniazid, with various alcohols, phenols and thiols, including several drugs, using carbodiimide-mediated coupling. Nineteen new esters were evaluated as potential antimycobacterial agents against drug-sensitive Mycobacterium tuberculosis (Mtb.) H37Rv, Mycobacterium avium and Mycobacterium kansasii. Selected derivatives were also tested for inhibition of multidrug-resistant (MDR) Mtb., and their mechanism of action was investigated. The esters exhibited high activity against Mtb. (minimum inhibitory concentrations, MIC, from &le;0.125 &mu;M), M. kansasii, M. avium as well as MDR strains (MIC from 0.25, 32 and 8 &micro;M, respectively). The most active mutual derivatives were derived from 4-chloro/phenoxy-phenols, triclosan, quinolin-8-ol, naphthols and terpene alcohols. The experiments identified enoyl-acyl carrier protein reductase (InhA), and thus mycobacterial cell wall biosynthesis, as the main target of the molecules that are activated by KatG, but for some compounds can also be expected adjunctive mechanism(s). Generally, the mutual esters have also avoided cytotoxicity and are promising hits for the discovery of antimycobacterial drugs with improved properties compared to parent isoniazid