High Efficacy of Combined Albendazole and Ivermectin Treatment Against Gastrointestinal Nematodes in Vervet Monkeys and Baboons

Conventional treatment that eliminates other gastrointestinal nematodes has failed to show adequate efficacy against Trichuris trichura in non-human primates (NHPs). We investigated the efficacy of albendazole and ivermectin against natural infestation of nematodes in non human primates. 18 vervet monkeys (Chlorocebus aethiops) and 21 baboons (Papio anubis) were divided into three treatment groups comprising of 6 vervets and 7 baboons per group. Albendazole (ABZ, 7.5mg/kg) was administered orally, and ivermectin (IVM, 300μg/kg) subcutaneously, each for three consecutive days. Group I animals were treated with a combination of albendazole and ivermectin, Group II ABZ alone, while Group III animals were treated with IVM alone. Faecal samples were collected at 0, 7, 14 and 28 days post treatment (dpt) and analysed for the presence of faecal eggs using the McMaster and formol ether acetyl (FEA) methods. Faecal egg count reduction percentage (FECR (%)) and cure rate (CR (%) i.e. percentage of faecal egg negative individuals after treatment) were used to determine the efficacy of the treatment regimens. The FEA method was found to be a more sensitive assessment method than the McMaster technique. When both methods were used the helminths observed included Trichuris trichura (100% in both NHPs) and strongyles (29.4% in vervets and 28.6% in baboons). In vervets, the FECR of T. trichura at 28 dpt was 100% (Group I), 75% (Group II) and 0% (Group III) while the CR (at the same time point) was 100% (Group I), 60% (Group II) and 0% (Group III). In baboons, the FECR% and CR% of T. trichura at 28dpt, for groups I, II, III was 100%, 100%, 0%, respectively. All the three drug regimens were curative (100%) of strongyles at 28 dpt. It is concluded that a combined ivermectin and albendazole treatment for 3 days is effective in treating T. trichura and strongyles infections in vervet monkeys and baboons. Further trials should be conducted using a bigger sample size as well as in other primates including humans

The prevalence of gastrointestinal nematode infection and their impact on cattle in Nakuru and Mukurweini districts of Kenya

A cross-sectional study was conducted in Nakuru and Mukurweini districts of Kenya to estimate the prevalence of gastrointestinal nematodes (GIN) and the financial impact of such infections among smallholder dairy farms. Parasitological examination involving feacal egg count and larval culture was employed to determine prevalence and burden of GIN. Questionnaires were administered to collect individual animal and farm management data. The impact of GIN infection on average daily milk production in lactating cows was also estimated using generalized linear regression analysis The prevalence of GIN infection was significantly different (p< 0.05) between Nakuru and Mukurweini, at 19.8% and 8.3%, respectively (13.8% overall), for a relative risk of infection of 2.3. Farm-level prevalence of infections were estimated at 28.1% (36/128) for Haemonchus, 19.5% (25/128) for Trichostrongylus and 14.8% (19/128) for Oesophagostomum. Average daily milk production in litres in the GIN-infected milking cows was 5.4 compared to 7.8 in the noninfected cows. GIN infection was associated with 1.4 litres per cow per day less milk and this difference was statistically significant (p< 0.05). The observed difference in milk production translated to a daily loss of 0.35 US dollars (USD) per cow per day at a cost of 0.25 USD per litre, which was the average farm gate price of milk at study time in the area under study.Keywords: Cattle, Gastrointestinal nematode infections, Milk production, Cross-sectional study

Anthelmintic and Other Pharmacological Activities of the Root Bark Extracts of Albizia anthelmintica Brongn

The anthelmintic activity of water, methanol and chloroform extracts of the root bark of Albizia anthelmintica on strongyle-type sheep nematode eggs and larvae were examined in vitro. In addition, pharmacological tests were carried out on the water extract to confirm other ethnomedical uses of the plant. The water extract inhibited hatching of the nematode eggs as well as development of larvae. It caused larval mortality at moderately high doses. The methanol extract had no effect on the eggs and on the development of larvae, but had high activity against survival of the larvae. The chloroform extract was the least active of the three extracts and it had moderate effect on larval development and larval survival. In addition, the water extract caused contraction of the smooth muscle of the guinea pig and rabbit ileum and the rat uterus. The water extract had negative inotropic and chronotropic effects and contractile effects on guinea pig trachea. The results support the ethnomedical use of this plant as an anthelmintic and for prevention of hemorrhage after birth. Key Words: Albizia anthelmintica, anthelmintic activity, pharmacology East and Central African Journal of Pharmaceutical Sciences Vol.5(3) 2002: 60-6

Siting Transmission Lines in a Changed Milieu: Evolving Notions of the "Public Interest" In Balancing State and Regional Considerations

This Article discusses how state public utility law presents a barrier to the siting of new high voltage transmission lines to serve renewable resources, and how states could approach its evolution in order to preserve a role for state regulators in a new energy economy in which renewable energy will play a significant role. The traditional approach to determining the "public interest" in siting transmission lines is well on its way to obsolescence. Two developments over the past fifteen years have begun to challenge this paradigm. First, policies at the federal level and in many states have encouraged increased competition in generation, contributing to de-monopolization of the bulk power side of the industry. Second, the increased emphasis on environmental, energy independence, and other public policy objectives, has resulted in a dramatically increased demand for renewable energy, particularly given heightened attention to climate change. Given that wind power -- the most economically viable renewable resource on a bulk power basis -- is feasible predominantly in locations far removed from, load centers, the demand for new multistate transmission facilities has been brought clearly into focus. After an introduction in Part I, Part II describes the existing arrangements in several resource rich Western states for siting new transmission lines, and the coexistence of those arrangements with a conventional understanding of the public interest in determining need and addressing environmental concerns under traditional state transmission siting laws. Part III discusses transmission issues related to the competitive wholesale market and increased attention to climate change and highlights how federal law has expanded to accommodate some of these concerns. Part IV emphasizes the need for a new definition of the public interest which might better reflect these new market circumstances and opportunities, and highlights the two main barriers to this: 1) legislative and/or regulatory inertia and 2) an outdated cost-allocation model. The public interest under most state siting statutes is sufficiently capacious to give regulators some flexibility to evolve, but in other instances legislative action may be needed. In addition, the state cost-of-service ratemaking model must evolve to a more regional approach to allocating the costs of new transmission

Strategic control of gastrointestinal nematodes of sheep in the highlands of central Kenya

The effectiveness of anthelmintic treatments given 3 weeks after the onset of rains to control gastrointestinal nematodes in sheep in the highlands of central Kenya was investigated. The study was carried out on a farm situated approximately 85 km north west of Nairobi in Nyandarua District of central Kenya. In May 1999, 35 Corriedale ram lambs aged between 8 and 10 months were ear-tagged, weighed and given albendazole at 3.8 mg/kg body mass. The animals were then allocated to three treatment groups. Three weeks after onset of both the short and long rains' season in November 1999 and April 2000 respectively, lambs in groups 1 and 2 were dewormed. Lambs in group 1 were given closantel at 10 mg/kg body mass in November and closantel plus albendazole at 3.8 mg/kg body mass in April. Lambs in group 2 were given albendazole at 3.8 mg/kg body mass on both occasions, while lambs in group 3 were maintained as the untreated controls. Nematode eggs per gram of faeces (epg) for lambs in the control group were significantly higher (P<0.05) than in the treated groups beginning from November, when the strategic treatments started. The levels of epg did not differ significantly between the two treated groups. Body mass for the treated groups were significantly higher (P<0.05) than for the control group from January 2000 until the experiment was terminated. The rainfall received in the study area in 2000 during the long rain season was inadequate and only occurred for a short period. The amount of herbage on pastures was therefore not adequate and all the study animals started losing mass from June 2000 until the experiment was terminated. The cumulative mass gain and amount of wool produced by the treated lambs during the study period did not differ significantly. There was therefore no difference in using either of the two drugs. It is concluded that, strategic anthelmintic treatments of sheep at the start of the wet season in the highlands of central Kenya is effective in controlling gastrointestinal nematodes. To prevent high levels of re-infection during the season of the long rains (April to June), it is recommended that, during this season, a second treatment be given 5-6 weeks after the first one or at the start of the dry season.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Food and Agriculture Organization (FAO) Technical Cooperation Programme Project No. FAO-TCP/KEN/8822 (Integrated Sustainable Parasite Control in Ruminants in Mixed Farming Systems in Kenya) at the University of Nairobi.mn201

The prevalence and economic importance of bovine fasciolosis in Kenya - an analysis of abattoir data

A retrospective study covering a period of 10 years (1990-1999) was carried out using post mortem meat inspection records at the Veterinary Department Headquarters at Kabete to determine the prevalence and economic importance of bovine fasciolosis in Kenya. Meat inspection records from abattoirs in 38 districts distributed over seven out of the eight provinces of Kenya were examined. Prevalence of fasciolosis was calculated as the number of cattle found to be infected with Fasciola, expressed as a percentage of the total number of cattle slaughtered. Using the average weight and market price of a bovine liver, the monetary loss occasioned by condemnation of Fasciola infected livers was calculated. A survey was also carried out at Dagoretti slaughterhouse complex in Nairobi to determine the relative occurrence of F. gigantica and F. hepatica in slaughtered cattle. Cattle slaughtered at Dagoretti slaughterhouse originate from all parts of the country. A total of 5 421 188 cattle were slaughtered in the seven provinces of Kenya during the 10-year period and 427 931 (8%) of these cattle were infected with Fasciola. The region with the highest prevalence of fasciolosis was Western Province (16%) followed, in descending order, by Eastern Province (11%), Nyanza Province (9%), Rift Valley Province (8%), Central Province (6%), Nairobi Province (4%) and Coast Province (3.5%). The total economic loss incurred by the country during the 10-year period as a result of condemnation of the infected livers was approximately US$2.6 million. The total annual economic losses during this period ranged from approximately US$0.2-0.3 million. The highest total economic losses for the 10-year period were recorded in Western Province (US$0.8 million) and Central Province (US$0.7 million). A total of 1 584 cattle originating from five provinces of Kenya were slaughtered at Dagoretti slaughterhouse over a period of two months of which 147 (9.3%) were infected with liver flukes. All the liver flukes obtained from the infected livers were identified as F. gigantica. It is concluded that fasciolosis is prevalent in cattle in all provinces of Kenya, that it causes great economic losses as a result of condemnation of infected livers, and that F. gigantica is the main species of liver flukes affecting cattle in Kenya. Local climatic factors, cattle trade, rustling and population numbers, and the presence of the snail intermediate hosts are probably the main factors influencing the incidence of the disease in the various regions of the country.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Analysis of small ruminants’ pastoral management practices as risk factors of peste des petits ruminants (PPR) spread in Turkana District, Kenya

Peste des petits ruminants (PPR) is an emerging viral disease spreading throughout Kenya and East Africa causing major losses in the small stock. This study is an attempt to evaluate small stock management practices in Turkana pastoral system, Kenya as predictors of PPR outbreaks. Information on the social practices and the occurrence of PPR outbreaks was obtained by participatory techniques. The small stock management practices, evaluated as factors, in a previous study were simultaneously analyzed with seasons and administrative divisions as the independent risk factors for the presence or absence of PPR outbreaks in 142 Adakars (villages) as the dependent variable. Analyses were carried out for the years 2009 and 2010 combined as one data set and considered as longitudinal repeated data. In the analyses, the presence or absence of PPR outbreaks was the dependent variable. Data were further analyzed separately disaggregated by season where the presence or absence of PPR outbreaks in a season was considered as the dependent variable. All analyses utilized multivariable logistical regression analyses. In the longitudinal analysis, season was the only significant factor associated with PPR outbreak. Disaggregating the data by season revealed that certain seasonal-specific livestock management activities increased the risk of reporting PPR outbreaks: (1) sharing water sources leading to social aggregation of young stock in one point (Factor 3) (odds ratio (OR) = 2.0) in season 2 (wet season) of 2009; (2) sick dams left to nurse their young kids/lambs (Factor 7) (OR=1.62) in the same season in 2010. The finding of diverse risk factors in the same seasons across years suggests temporal heterogeneity in the distribution and occurrence of the determinants of PPR in the Turkana ecosystem. The study discusses the implications of these findings on disease control

Hollow pellet injection for magnetic fusion

Precise delivery of mass to burning plasmas is a problem of growing interest in magnetic fusion. The answers to how much mass is necessary and sufficient can vary depending on parameters such as the type of atoms involved, the type of applications, plasma conditions, mass injector, and injection timing. Motivated by edge localized mode (ELM) control in H-mode plasmas, disruption mitigation and other applications in magnetic fusion, we report progress and new possibilities in mass delivery based on hollow pellets. Here, a hollow pellet refers to a spherical shell mass structure with a hollow core. Based on an empirical model of pellet ablation, coupled with BOUT++ simulations of ELM triggering threshold, hollow pellets are found to be attractive in comparison with solid spheres for ELM control. By using hollow pellets, it is possible to tailor mass delivery to certain regions of edge plasmas while minimizing core contamination and reducing the total amount of mass needed. We also include experimental progress in mass delivery experiments, in-situ diagnostics and hollow pellet fabrication, and emphasize new experimental possibilities for ELM control based on hollow pellets. A related application is the disruption mitigation scheme using powder encapsulated inside hollow shells. Further experiments will also help to resolve known discrepancies between theoretical predictions and experiments in using mass injection for ELM control and lead to better predictive models for ELM stability and triggering.Comment: Manuscript prepared for reviews by {\it Nuclear Fusion}, following the initial presentation in the 27th IAEA FEC, Gandhinagar, India, Oct. 22 - 27, 201

A Fast Visible Camera Divertor-Imaging Diagnostic on DIII-D

In recent campaigns, the Photron Ultima SE fast framing camera has proven to be a powerful diagnostic when applied to imaging divertor phenomena on the National Spherical Torus Experiment (NSTX). Active areas of NSTX divertor research addressed with the fast camera include identification of types of EDGE Localized Modes (ELMs)[1], dust migration, impurity behavior and a number of phenomena related to turbulence. To compare such edge and divertor phenomena in low and high aspect ratio plasmas, a multi-institutional collaboration was developed for fast visible imaging on NSTX and DIII-D. More specifically, the collaboration was proposed to compare the NSTX small type V ELM regime [2] and the residual ELMs observed during Type I ELM suppression with external magnetic perturbations on DIII-D[3]. As part of the collaboration effort, the Photron camera was installed recently on DIII-D with a tangential view similar to the view implemented on NSTX, enabling a direct comparison between the two machines. The rapid implementation was facilitated by utilization of the existing optics that coupled the visible spectral output from the divertor vacuum ultraviolet UVTV system, which has a view similar to the view developed for the divertor tangential TV camera [4]. A remote controlled filter wheel was implemented, as was the radiation shield required for the DIII-D installation. The installation and initial operation of the camera are described in this paper, and the first images from the DIII-D divertor are presented

Magnetic Field Line Tracing Calculations for Conceptual PFC Design in the National Compact Stellarator Experiment

The National Compact Stellarator Experiment (NCSX) is a three-field period compact stellarator presently in the construction phase at Princeton, NJ. The design parameters of the device are major radius R=1.4m, average minor radius &lt;a&gt; = 0.32m, 1.2 {le} toroidal field (B{sub t}) {le} 1.7 T, and auxiliary input power up to 12 MW with neutral beams and radio-frequency heating. The NCSX average aspect ratio &lt;R/a&gt; of 4.4 lies well below present stellarator experiments and designs, enabling the investigation of high {beta} physics in a compact stellarator geometry. Also the NCSX design choice for a quasi-axisymmetric configuration aims toward the achievement of tokamak-like transport. In this paper, we report on the magnetic field line tracing calculations used to evaluate conceptual plasma facing component (PFC) designs. In contrast to tokamaks, axisymmetric target plates are not required to intercept the majority of the heat flux in stellarators, owing to the nature of the 3-D magnetic field footprint. The divertor plate design investigated in this study covers approximately one half of the toroidal extent in each period. Typical Poincare plots in Figure 1 illustrate the plasma cross-section at several toroidal angles for a computed NCSX high-beta equilibrium. The plates used for these calculations are centered in each period about the elongated cross-section shown in Figure 1a, extending to +/- {pi}/6 in each direction. Two methods for tracing the edge field line topology were used in this study. The first entails use of the VMEC/MFBE-2001 packages, whereas the second entails use of the PIES code with a post-processor by Michael Drevlak; the same field line integration routine was used to evaluate the equilibria for this comparison. Both inputs were generated based on the {beta}=4%, =iota=0.5 equilibrium computed from the final NCSX coil set. We first compare these two methods for a specific plate geometry, and conclude with a comparison of the strike characteristics for two different target plate poloidal lengths using the latter method. The details of the magnetic topology differ when computed with VMEC/MFBE as compared with an iterated PIES solution. This difference is illustrated in Figure 2. The presence of islands in the PIES solution effectively reduces the radius of the last closed magnetic surface (LCMS) by about 8 cm. As expected, this difference in the edge topology translates to a difference in field line terminations