COVID-19 disruptions and pivoting in SMEs in the hidden middle of Kenya’s potato and fish value chains

Small and medium enterprises (SMEs) in Kenya were severely affected by the COVID-19 pandemic and restrictions put in place to contain it. This study assesses the impact of these restrictions on the midstream of the potato and fish value chains, with a focus on traders and processors, and investigates the short- and longer-term responses and ‘pivoting’ strategies deployed by these firms. Longitudinal data were collected from 937 respondents with three recall periods: (1) the pre-pandemic situation (2019); (2) the period of strictest restrictions, necessitating immediate adaptations by firms (2020); and (3) a period of anticipated recovery with longer-term business adaptation strategies (2021). Firms in both value chains reported a dramatic drop in turnover in 2020. Potato SMEs partially recovered in 2021, but fish SMEs reported further declines in turnover. In the face of restrictions, SMEs shifted toward more localized procurement and sales, shorter supply chains (often by-passing intermediaries and increasing use of contracts), use of smaller vehicles, and toward the adoption of information and communication technology (ICT) to search for and engage with business partners. Smaller firms and the biggest of businesses were more resilient and less subject to large fluctuations in business turnover, suggesting an inverted U-shape relationship between firm size and impact. Firms located close to production areas and selling primarily within their own county were also less affected. We provide recommendations for increasing business resilience to shocks based on these findings

Comprehensive transcriptome of the maize stalk borer, Busseola fusca, from multiple tissue types, developmental stages, and parasitoid wasp exposures

International audienc

COVID-19 disruptions and pivoting in SMEs in the hidden middle of Kenya’s potato and fish value chains

Small and medium enterprises (SMEs) in Kenya were severely affected by the COVID-19 pandemic and restrictions put in place to contain it. This study assesses the impact of these restrictions on the midstream of the potato and fish value chains, with a focus on traders and processors, and investigates the short- and longer-term responses and ‘pivoting’ strategies deployed by these firms. Longitudinal data were collected from 937 respondents with three recall periods: (1) the pre-pandemic situation (2019); (2) the period of strictest restrictions, necessitating immediate adaptations by firms (2020); and (3) a period of anticipated recovery with longer-term business adaptation strategies (2021). Firms in both value chains reported a dramatic drop in turnover in 2020. Potato SMEs partially recovered in 2021, but fish SMEs reported further declines in turnover. In the face of restrictions, SMEs shifted toward more localized procurement and sales, shorter supply chains (often by-passing intermediaries and increasing use of contracts), use of smaller vehicles, and toward the adoption of information and communication technology (ICT) to search for and engage with business partners. Smaller firms and the biggest of businesses were more resilient and less subject to large fluctuations in business turnover, suggesting an inverted U-shape relationship between firm size and impact. Firms located close to production areas and selling primarily within their own county were also less affected. We provide recommendations for increasing business resilience to shocks based on these findings

Assessment of Cu, Zn, Mn, and Fe enrichment in Mt. Kenya soils: evidence for atmospheric deposition and contamination

Mountains are the preferred sites for studying long-range atmospheric transportation and deposition of heavy metals, due to their isolation and steep temperature decrease that favors cold trapping and condensation of particulate forms of heavy metals. Any enrichment of heavy metals in mountains is presumed to primarily occur through atmospheric deposition. In this particular study, we assessed the status of 27 subsurface soils collected along two elevation gradients of Mt. Kenya using enrichment factors (EFs) as the ecological risk assessments. The collected soils were analyzed for total organic carbon, zinc (Zn), iron (Fe), manganese (Mn), and copper (Cu). The mean concentration of Mn, Fe, Zn, and Cu was 0.376 mg/kg, 47.6 mg/kg, 12.3 mg/kg, and 4.88 mg/kg in Chogoria and 0.560 mg/kg, 113 mg/kg, 12.7 mg/kg, and 2.70 mg/kg in Naro Moru respectively. These concentrations were below the US-EPA maximum permissible levels for soils, implying that the levels recorded had low toxicity. Meanwhile, the mean enrichment factors for Mn, Cu, and Zn were 0.447, 131, and 78.8 in Chogoria and 0.463, 38.9, and 53.0 in Naro Moru respectively. This implied that Zn and Cu in Chogoria sites were extremely enriched, while in Naro Moru, enrichment levels ranged from significant to extreme. However, Mn was found to have minimal enrichment in all the sites. Lower montane forest and bamboo zone recorded relatively high enrichment due to distance from source of pollution. Ericaceous zone also had high mean enrichment due to influence of wind which favors higher deposition at mid-elevations

Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection

Globally, the emergence of the coronavirus disease (COVID-19) has had a significant impact on life. The need for ongoing SARS-CoV-2 screening employing inexpensive and quick diagnostic approaches is undeniable, given the ongoing pandemic and variations in vaccine administration in resource-constrained regions. This study presents results as proof of concept to use hybridization chain reaction (HCR) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a complex for detecting SARS-CoV-2. HCR hairpin probes were designed using the NUPACK web-based program and further used to amplify the SARS-CoV-2 N gene in archived nasopharyngeal samples. The results were visualized using agarose gels and CRISPR Cas12a-based lateral flow strips. The assay was evaluated using the gold standard, real-time polymerase chain reaction (RT-PCR), as recommended by the World Health Organization (WHO). The results show the comparative efficiency of HCR to RT-PCR. This study shows that HCR and CRISPR are viable alternatives for diagnosing SARS-CoV-2 in samples

Disparities in Dolutegravir Uptake Affecting Females of Reproductive Age With HIV in Low- and Middle-Income Countries After Initial Concerns About Teratogenicity : An Observational Study.

BACKGROUND The transition to dolutegravir-containing antiretroviral therapy (ART) in low- and middle-income countries (LMICs) was complicated by an initial safety signal in May 2018 suggesting that exposure to dolutegravir at conception was possibly associated with infant neural tube defects. On the basis of additional evidence, in July 2019, the World Health Organization recommended dolutegravir for all adults and adolescents living with HIV. OBJECTIVE To describe dolutegravir uptake and disparities by sex and age group in LMICs. DESIGN Observational cohort study. SETTING 87 sites that began using dolutegravir in 11 LMICs in the Asia-Pacific; Caribbean, Central and South America network for HIV epidemiology (CCASAnet); and sub-Saharan African regions of the International epidemiology Databases to Evaluate AIDS (IeDEA) consortium. PATIENTS 134 672 patients aged 16 years or older who received HIV care from January 2017 through March 2020. MEASUREMENTS Sex, age group, and dolutegravir uptake (that is, newly initiating ART with dolutegravir or switching to dolutegravir from another regimen). RESULTS Differences in dolutegravir uptake among females of reproductive age (16 to 49 years) emerged after the safety signal. By the end of follow-up, the cumulative incidence of dolutegravir uptake among females 16 to 49 years old was 29.4% (95% CI, 29.0% to 29.7%) compared with 57.7% (CI, 57.2% to 58.3%) among males 16 to 49 years old. This disparity was greater in countries that began implementing dolutegravir before the safety signal and initially had highly restrictive policies versus countries with a later rollout. Dolutegravir uptake was similar among females and males aged 50 years or older. LIMITATION Follow-up was limited to 6 to 8 months after international guidelines recommended expanding access to dolutegravir. CONCLUSION Substantial disparities in dolutegravir uptake affecting females of reproductive age through early 2020 are documented. Although this disparity was anticipated because of country-level restrictions on access, the results highlight its extent and initial persistence. PRIMARY FUNDING SOURCE National Institutes of Health