Serum anti-hsp27 antibodies concentration in diabetes mellitus; population based case-control study

Background: Diabetes mellitus is an important risk factor for cardiovascular disease. Different biomarkers have been investigated for the diagnosis of diabetes pathogenesis or its complications. There are also reports regarding an increased level of anti-HSP27 antibodies in atherogenesis. We aimed to evaluate serum anti-heat shock protein 27 antibodies level in subjects with diabetes mellitus and undiagnosed individuals.  Materials and Methods: This cross-sectional study was conducted on 6447 MASHAD study subjects, including four groups with diabetes mellitus (n=610), undiagnosed diabetes (n=162), impaired fasting glucose (IFG) (n=619) and normal (n=5056) subjects. Demographic and anthropometric data were obtained from all participants. Fasting serum glucose (FSG) and other parameters were measured. In-house enzyme-linked immune sorbent assay method was used for measuring Anti-HSP27 antibodies levels. Results: There were significant differences in weight (p=0.034), body mass index, waist, and hip circumference, systolic and diastolic blood pressure, fasting serum glucose, lipid profile and high sensitive- C reactive protein (p<0.001) between four groups of diabetes mellitus, undiagnosed diabetes, impaired fasting glucose, and normal subjects. The serum anti-HSP27 antibody titer did not show a significant difference between studied groups. Conclusion: Serum antibody titers to HSP27 were not significantly different between four groups categorized based on their FSG levels in a large population

A genetic variant in the cytochrome P450 family 2 subfamily R member 1 determines response to vitamin D supplementation

Background Globally, about 1 billion people have inadequate levels of serum vitamin D and it is prevalent in all ethnicities and age groups. Few foods naturally contain sufficient vitamin D; therefore, most people get their requirements through supplementation. Hence vitamin D status is affected by genetic and environmental determinants including season of measurement, diet habitual, health status, body mass index and concurrent medication. Further studies are necessary to understand how genetic variation influences vitamin D metabolism. We aimed to explore the association between a potential vitamin D-related polymorphism (the rs10766197 polymorphism in the CYP2R1 gene) with the response to supplementation of vitamin D in 253 healthy Iranian girls. Material and method A total of 253 healthy subjects received 50,000 IU of vitamin D3 weekly for 9 weeks. Serum 25(OH)D concentrations and metabolic profiles were measured at baseline and after 9 weeks of supplementation. The genotypes of the CYP2R1 variant (rs10766197) were identified using TaqMan genotyping assays. Results Serum 25(OH)D during the supplementation, increased in all individuals. Subjects with a AA major genotype at this locus had higher vitamin D concentrations after intervention (Changes (%) 448.4% ± 425% in AA vs 382.7% ± 301% in GG). This genetic variant modulated the response to supplementation (p < 0.001 and p-value SNP = 0.05). Regression analysis showed that the probability of affecting serum 25(OH)D, in individuals who had homozygous major allele GG was two-fold higher than carriers of the uncommon allele A (OR = 2.1 (1–4.2); p = 0.03). Interestingly, the Hs-CRP was reduced in AA carries while was elevated in individuals with GG and AG genotypes, after high-dose vitamin D supplementation. Conclusion Changes in serum vitamin D and metabolic profile following high dose supplementation with vitamin D were associated with CYP2R1 polymorphism. Although carriers of the common G allele showed a greater response in the serum vitamin D

Metabolic syndrome components as markers to prognosticate the risk of developing chronic kidney disease: evidence-based study with 6492 individuals

Objective The global prevalence of metabolic syndrome (MetS) appears to be increasing and the impact of this condition on potential comorbidities such as cardiovascular disease is high. Chronic kidney disease (CKD) is also a potential comorbidity of MetS but the method of screening for this is somewhat controversial. Thus, predictive markers that can predict the risk of developing CKD are warranted for identification of patients with MetS at an increased risk. Research methods/patients We investigated the occurrence of CKD in 6492 individuals, either with or without MetS. Results Our results showed that the prevalence of CKD was markedly higher in those individuals with MetS, and increased progressively with the number of MetS components and age. Waist circumference, triglycerides and high-density lipoprotein cholesterol were significantly (p<0.05) associated with altered levels of urea nitrogen, glomerular filtration rate and creatinine, and were related to the increased risk of CKD (eg, OR 1.293 (95 CI 1.10 to 1.52; p=0.002)). The relative risk of CKD remained statistically significant for uric acid following multivariate analyses and adjusting for MetS-associated factors. Conclusions Our data demonstrated the association of MetS components with CKD in our population and revealed that susceptibility to CKD was increased with the number of defining features of MetS. These findings prompt prospective studies to determine the impact of preventing and detecting MetS on the risk of developing CKD. © 2015 by the BMJ Publishing Group Ltd