Comparison the frequency of dry eye syndrome in patients with newly diagnosed rheumatoid arthritis with healthly people in Zahedan

زمینه و هدف: از شایع ترین تظاهرات درگیری چشم، سندرم چشم خشک می باشد. سندرم چشم خشک باعث افزایش خطر عفونت چشم و اختلال در میزان دقت بینایی می گردد. مطالعات مختلف نتایج متفاوتی در خصوص شیوع آرتریت روماتویید و سندرم چشم خشک نشان داده اند. به نظر می رسد یکی از علل تفاوت شیوع سندرم چشم خشک در مطالعات مختلف، تفاوت آب و هوایی در مناطق مورد مطالعه باشد. هدف از این مطالعه، بررسی میزان شیوع خشکی چشم در بیماران مبتلا به آرتریت روماتویید در مناطق با آب و هوای گرم و خشک در مقایسه با افراد سالم و همچنین بررسی میزان شیوع سندرم چشم خشک در مبتلایان به آرتریت روماتویید تازه تشخیص داده شده می باشد. روش بررسی: در این مطالعه مورد-شاهدی،60 نفر از مبتلایان به آرتریت روماتویید و 60 نفر از افراد سالم که از نظر سن و جنس همسان سازی شده بودند وارد مطالعه شدند و در آنها علاوه بر شرح حال و علایم بالینی تست های شیرمر، منیسک اشک، فلورسین و شکست اشک بررسی شد. در بیمارانی که یکی یا بیشتر از این تست ها مثبت بود، تشخیص سندرم چشم خشک داده می شد. داده ها با استفاده از آزمون آماری کای اسکوئر مورد تجزیه و تحلیل قرار گرفتند. یافته‌ها :از 60 نفر مبتلا به آرتریت روماتویید 32 نفر (53) مبتلا به سندرم چشم خشک قطعی و 15 نفر (25) مبتلا به سندرم چشم خشک احتمالی بودند و در گروه کنترل 6 نفر (10) سندرم چشم خشک داشتند بین دو گروه بیمار و کنترل از نظر وجود سندرم چشم خشک قطعی اختلاف آماری معنی داری وجود داشت (001/0

Association of FAS and FAS Ligand Genes Polymorphism and Risk of Systemic Lupus Erythematosus

FAS/FASL pathway plays a critical role in maintaining peripheral immune tolerance; therefore, the apoptosis genes, Fas and Fas ligand (FasL), could be suitable candidate genes in human SLE susceptibility. Materials and Methods. In this case-control study, 106 SLE patients and 149 sex, age, and ethnicity matched healthy controls were genotyped for the Fas A-670G and FasLC-844T polymorphisms by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). Results. The frequency of -670AA genotype was significantly higher in SLE patients than control group and the risk of SLE was 2.1-fold greater in subjects with AA genotype (P=0.03). The frequency of -670A allele was significantly higher in SLE patients than in controls too (58% versus 49%, P=0.03). The -844CC genotype frequency was significantly higher in SLE patients than in healthy controls and the risk of SLE was 2.8-fold greater in these subjects (P=0.01). The C allele frequency was significantly higher in patients than in controls (69% versus 49%, P=0.001). Increased SLE risk was observed in individuals with combined effect of Fas-670AA and FasL-844CC genotypes (P=0.001). Conclusion. Fas-670AA and FasL-844CC genotypes were associated with SLE risk, and combined effect of -670AA and -844CC genotypes might increase SLE susceptibility

Prooxidant-Antioxidant Balance in Patients with Systemic Lupus Erythematosus and Its Relationship with Clinical and Laboratory Findings

Aim. This study was aimed at evaluating prooxidant-antioxidant balance (PAB) in patients with systemic lupus erythematosus (SLE) and its relationship with laboratory findings and clinical manifestations. Methods. In this case-control study, 60 patients with SLE and 60 healthy individuals were enrolled. The blood samples were collected and their sera were separated. Subsequently, the prooxidant-antioxidant balance value was evaluated using PAB assay for each sample. Results. The mean of PAB values in SLE patients was significantly higher than healthy controls (147.3±42 versus 84.8±32.2 HK, P<0.0001). Furthermore, in SLE patients, there was a positive significant correlation between the PAB and erythrocyte sedimentation rate (ESR) (r=0.492, P<0.001). In addition, the PAB values in patients with alopecia, discoid rash, oral ulcers, arthritis, and nephritis were significantly higher than those without these manifestations. Conclusion. The findings of current study showed that the mean of PAB was significantly higher in SLE patients and PAB was correlated with ESR. Moreover increased PAB was found in SLE patients with alopecia, discoid rash, oral ulcers, arthritis, and nephritis. These findings suggest that the measurement of PAB may be useful to show oxidative stress condition in SLE patients

Frequency of HLA-DRB1 alleles in rheumatoid arthritis patients in Zahedan, southeast Iran

Background and Objectives: Analysis of the role of different alleles of human leukocyte antigen (HLA) in rheumatoid arthritis (RA) patients is necessary in many populations and geographical areas. The aim of the present study was to investigate the frequency of HLA-DRB1 alleles in RA patients, comparing with that in control group in southeast Iran. Design and Setting: Case-control study of rheumatoid arthritis patients referred to rheumatology clinic at university hospital. Patients and Methods: The frequency of HLA-DRB1 alleles was determined in 79 RA patients and 93 healthy subjects in Zahedan, southeast Iran. HLA-DRB1 allele types were identified by polymerase chain reaction with sequence-specific primer (PCR-SSP). Results: The HLA-DRB1FNx0110 allele showed a significantly higher frequency in patients with RA (OR=2.698, 95&#x0025; CI=1.087-6.699, P=.045), while the frequency of DRB1FNx0103 allele in these subjects was significantly lower than that in the control group (OR=0.447, 95&#x0025; CI=0.2285-0.8729, P=.021). The frequencies of DRB1FNx0101, DRB1FNx0104, DRB1FNx0107, DRB1FNx0109, DRB1FNx0111, DRB1FNx0113, DRB1FNx0114, DRB1FNx0115, DRB1FNx0116 were not significantly different between RA subjects and the control group. Conclusion: The data suggest that the DRB1FNx0110 allele is a risk factor and DRB1FNx0103 is protective for RA in this population

Evaluation of HLA-G 14 bp Ins/Del and +3142G&gt;C Polymorphism with Susceptibility and Early Disease Activity in Rheumatoid Arthritis

properly cited. Purpose/Background. Mounting evidence designates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases. There are controversial reports concerning the impact of HLA-G gene polymorphism on rheumatoid arthritis (RA). This study was aimed at examining the impact of 14 bp ins/del and +3142G&gt;C polymorphism with susceptibility and early disease activity in RA patients in a sample of the Iranian population. Methods. This case-control study was done on 194 patients with RA and 158 healthy subjects. The HLA-G rs1063320 (+3142G&gt;C) and rs66554220 (14 bp ins/del) variants were genotype by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively. Results. The HLA-G +3142G&gt;C polymorphism significantly decreased the risk of RA in codominant (OR = 0.61, 95% CI = 0.38-0.97, = 0.038, GC versus GG; OR = 0.36, 95% CI = 0.14-0.92, = 0.034, CC versus GG), dominant (OR = 0.56, 95% CI = 0.36-0.87, = 0.011, GC + CC versus GG), and allele (OR = 0.58, 95% CI = 0.41-0.84, = 0.004, C versus G) inheritance models tested. Our finding did not support an association between HLA-G 14 bp ins/del variant and risk/protection of RA. In addition, no significant association was found between the polymorphism and early disease activity. Conclusion. In summary, our results showed that HLA-G +3142G&gt;C gene polymorphism significantly decreased the risk of RA in a sample of the Iranian population

Evaluation of HLA-G 14 bp Ins/Del and +3142G>C Polymorphism with Susceptibility and Early Disease Activity in Rheumatoid Arthritis

Purpose/Background. Mounting evidence designates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases. There are controversial reports concerning the impact of HLA-G gene polymorphism on rheumatoid arthritis (RA). This study was aimed at examining the impact of 14 bp ins/del and +3142G>C polymorphism with susceptibility and early disease activity in RA patients in a sample of the Iranian population. Methods. This case-control study was done on 194 patients with RA and 158 healthy subjects. The HLA-G rs1063320 (+3142G>C) and rs66554220 (14 bp ins/del) variants were genotype by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFP) and PCR method, respectively. Results. The HLA-G +3142G>C polymorphism significantly decreased the risk of RA in codominant (OR = 0.61, 95% CI = 0.38–0.97, p=0.038, GC versus GG; OR = 0.36, 95% CI = 0.14–0.92, p=0.034, CC versus GG), dominant (OR = 0.56, 95% CI = 0.36–0.87, p=0.011, GC + CC versus GG), and allele (OR = 0.58, 95% CI = 0.41–0.84, p=0.004, C versus G) inheritance models tested. Our finding did not support an association between HLA-G 14 bp ins/del variant and risk/protection of RA. In addition, no significant association was found between the polymorphism and early disease activity. Conclusion. In summary, our results showed that HLA-G +3142G>C gene polymorphism significantly decreased the risk of RA in a sample of the Iranian population

Arg399Gln and Arg194Trp Polymorphisms and Risk of Systemic Lupus Erythematosus in an Iranian Population: A Pilot Study

Background. Evidences are suggesting that DNA damage is implicated in development of systemic lupus erythematosus (SLE). Therefore we focused on two common XRCC1 polymorphisms (Arg399Gln and Arg194Trp) in SLE susceptibility in South East of Iran. Methods. Peripheral blood DNA was extracted from 163 SLE patients and 180 healthy controls. PCR-restriction fragment length polymorphism method was used for genotyping of XRCC1 Arg399Gln and Arg194Trp polymorphisms. Results. The frequency of Arg/Gln genotype of the XRCC1 Arg399Gln polymorphism was significantly lower in SLE patients than controls. Moreover, lower frequency of Arg/Gln genotype was found in SLE patients with malar rash compared to patients without this manifestation. No association was observed between XRCC1 Arg194Trp polymorphism and increased risk of SLE in studied population. Haplotype analysis revealed no correlation between four haplotypes of XRCC1 Arg399Gln and Arg194Trp polymorphisms and SLE risk. Conclusion. These findings suggest that XRCC1 399 Arg/Gln heterozygous genotype plays a protective role in SLE susceptibility

Interleukin-1β (IL-1β) & IL-4 gene polymorphisms in patients with systemic lupus erythematosus (SLE) & their association with susceptibility to SLE

Background & objectives: Interleukin-1 (IL-1) is one of the pro-inflammatory cytokines that plays a main role in the regulation of immune and inflammatory responses. Interleukin 4 (IL-4) as an anti-inflammatory cytokine regulates balance between Th1 and Th2 immune responses. this study was undertaken to investigate the IL-1β and IL-4 genes polymorphisms in patients with systemic lupus erythematosus (SLE) and also association between the polymorphisms and susceptibility to SLE. Methods: One hundred and sixty three SLE patients and 180 healthy controls were genotyped for the IL-4 VNTR (variable number tandem repeat), IL-1β C-511T and IL-1β T-31C polymorphisms by polymerase chain reaction (PCR) or PCR-RFLP (restriction fragment length polymorphism) method. Results: The frequencies of CC genotype and C allele of the IL-1β T-31C polymorphism were significantly (P<0.01) lower in SLE patients than controls. Moreover, the frequencies of RP1/RP2 genotype and RP2 allele of IL-4 VNTR polymorphism were significantly (P<0.05) higher in the SLE patients. No association was observed between IL-1β C-511T polymorphism and increased risk of SLE. We observed increased frequency of CT and TT genotypes of IL-1β C-511T polymorphism in SLE patients with malar rash compared to SLE patients without this manifestation. Interpretation & conclusions: The present findings suggest that IL-1β T-31C and IL-4 VNTR polymorphisms but not IL-1β C-511T polymorphism may contribute in SLE pathogenesis. In addition, CT and TT genotypes of IL-1β C-511T polymorphism were associated with SLE