Fixed drug eruption resulting from fluconazole use: a case report

<p>Abstract</p> <p>Introduction</p> <p>Fluconazole is a widely used antifungal agent with a possible side effect of fixed drug eruption. However, this adverse drug effect is absent from the reported list of possible side effects of fluconazole. We are presenting a rare case in our report.</p> <p>Case presentation</p> <p>A 25-year-old Iranian woman developed fixed drug eruptions on different sites of her body after taking five doses of fluconazole to treat vaginal candidiasis. A positive patch test, positive oral challenge test and skin biopsy were all found to be consistent with fixed drug eruption.</p> <p>Conclusion</p> <p>Fluconazole is a widely prescribed drug, used mainly to treat candidiasis. Fixed drug eruption as a possible side effect of Fluconazole is not well known and thus, the lesions may be misdiagnosed and mistreated. Based on our findings, which are consistent with a number of other practitioners, we recommend adding fixed drug eruption to the list of possible side effects of fluconazole.</p

Preparation of a naturally driven cotton wound dressing via honey, Tragacanth and Sumac

An antibacterial wound dressing with wound healing effects of honey (H), Tragacanth Gum (TG) and Sumac (S) (Rhus coriaria L.) has been prepared. The antibacterial properties of five different concentrations of water extracted Sumac has been examined to find out the best sample. Ratios of honey and Tragacanth Gum are investigated along with the chosen concentration of Sumac in order to find out the optimum compound with desirable antibacterial and healing effects. The results of the well diffusion test indicate antibacterial activities against S. aureus and E. coli on all samples. Scratch test results demonstrate improvement in the proliferation of fibroblasts on the fabric treated with compounds. The prepared wound dressing accelerates the healing process and eliminates bacterial growth causing wound infection.

Preparation of a naturally driven cotton wound dressing via honey, Tragacanth and Sumac

260-266An antibacterial wound dressing with wound healing effects of honey (H), Tragacanth Gum (TG) and Sumac (S) (Rhus coriaria L.) has been prepared. The antibacterial properties of five different concentrations of water extracted Sumac has been examined to find out the best sample. Ratios of honey and Tragacanth Gum are investigated along with the chosen concentration of Sumac in order to find out the optimum compound with desirable antibacterial and healing effects. The results of the well diffusion test indicate antibacterial activities against S. aureus and E. coli on all samples. Scratch test results demonstrate improvement in the proliferation of fibroblasts on the fabric treated with compounds. The prepared wound dressing accelerates the healing process and eliminates bacterial growth causing wound infection

Identification of Candida Species Associated with Vulvovaginal Candidiasis by Multiplex PCR

Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. Methods. 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region were amplified. The multiplex PCR products were separated by electrophoresis in 2% agarose gel, visualized by staining with ethidium bromide, and photographed. Descriptive statistics, Chi-square test, and Spearman correlation were used to summarize the findings. Results.&nbsp; C. albicans and C. glabrata were the most common species isolated from the specimens. A mix of C. glabrata and C. albicans was the most common mixed infection isolated from the samples. The analysis revealed a significant positive association between older age and infection with C. glabrata isolates (Spearman’s rho = 0.89, P=0.015). Conclusion. Multiplex PCR is a fast, yet reliable method to identify Candida species. C. albicans and then C. glabrata are the two most common causes of vulvovaginal candidiasis. The number of mixed fungal infections is higher among Iranian population compared to international reports

Expression of TGF-β3 in Isolated Fibroblasts from Foreskin

Background: The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-β3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF-β3 gene (tgf-b3) in human foreskin fibroblasts (HFF’s). Methods: We obtained HFF’s from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF-β3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-β3 was measured by enzyme-linked immunosorbent assay (ELISA). Results: The highest TGF-β3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 μl of transfection reagent. Expression was 1.4-fold greater than control by electroporation. Conclusions: In this study, we successfully increased TGF-β3 expression in primary fibroblast cells. In the future, grafting these transfected fibroblasts onto wounds can help the healing process without scarring