Genetic variability of camel (Camelus dromedarius) populations in Saudi Arabia based on microsatellites analysis

The genetic diversity and population genetic structure of dromedary camels (Camelus dromedarius) are poorly documented in Saudi Arabia. The present study was conducted to address some of these genetics using four Saudi Arabian camel populations namely; Magaheem (MG), Maghateer (MJ), Sofr (SO) and Shual (SH). Genomic DNA was extracted from the hair roots of 160 camels, 40 individuals from each population. Sixteen microsatellite markers were used to genotype these 160 camels. Out of these 16 markers, only microsatellite VOLP67 did not produce any polymerase chain reaction (PCR) amplicons. There were 139 alleles generated by the 15 microsatellites loci with a mean of 9.27 alleles per locus. Four of the microsatellites loci studied in MG, eight in MJ and six in both SO and SH were found to be deviated from Hardy-Weinberg equilibrium. The fixation genetic indices (Fst) among the four populations were very low, ranging from 0.006 (between SH and SO) to 0.017 (between MG and MJ), indicating low population differentiation among the four Saudi camel populations. No significant heterozygote excess or bottleneck in most nearest past was detected in the four camel populations as indicated by sign, standardized differences and Wilcoxon tests, along with the normal L shaped distribution of mode-shift test. The present study showed that the microsatellite markers are powerful tools in breeding programs, although there is a need for applying more microsatellites in order to be able to discriminate fairly between camel populations of Saudi Arabia.Keywords: Camels, Camelus dromedarius, microsatellite markers, Saudi Arabia, genetic variabilit

Attitude and Practice of Pharmacy Students Toward Traditional Herbal Medicines, Sudan

Ebaa I Mahmoud,1 Mariam A Yousif,1 Ahmed H Arbab2 1Faculty of Pharmacy, University of Khartoum, Khartoum, 11111, Sudan; 2Department of Pharmacognosy, Faculty of Pharmacy, University of Khartoum, Khartoum, 11111, SudanCorrespondence: Ahmed H Arbab, Department of Pharmacognosy, Faculty of Pharmacy, University of Khartoum, Al-Qasr Ave, Khartoum, 11111, Sudan, Tel +249 991893200, Email [email protected]: Traditional herbal medicines (THMs) are widely used in Sudan, but there is scarcity of research on pharmacy students’ attitudes and utilization of THMs. This study aims to assess the attitudes and practices of pharmacy students toward THM.Methods: A descriptive cross-sectional study was conducted among randomly selected undergraduate pharmacy students at the University of Khartoum (Sudan). Data were collected using a validated self-administered questionnaire adapted from previous studies. A statistical package for the social sciences was used for data analysis.Results: Among the 264 respondents, the average age was 21.8 years, and 84.8% of them were females. Nearly 85% of respondents were residents of Khartoum. The overall respondent’s attitudes towards THMs were positive, it ranged from high (19.7%), moderate (79.9%). About 87.8% of respondents are interested in learning more about THMs. In addition, 36.4%, and 50.4% of respondents strongly agreed or agreed that THMs should be officially licensed and controlled. A 17.8% and 63.3% of respondents, respectively, reported using traditional herbal remedies either always or occasionally. Supermarkets (35.2%) and friends/family (36.4%) were the main sources of THMs for the participants, and oral administration was the most used route (87.1%). More than 90% of respondents said they would suggest THMs to others, and the most common justifications for doing so were their accessibility (46.6%) and affordability (16.3%). The most widely used herbs were Mentha spicata, Zingiber officinale, Acacia nilotica, Hibiscus sabdariffa, and Syzygium aromaticum. Data revealed a significant association between attitude levels and respondents’ gender (p=0.046), year of study (p=0.000), and residence (p=0.017).Conclusion: Most undergraduate pharmacy students had a positive attitude and utilized THMs. Development of effective educational initiatives, research, and regulations are recommended to ensure the appropriate utilization of traditional herbal remedies.Keywords: traditional herbal medicines, attitude, practice, pharmacy students, Suda

Effect of Aspect Ratio on Flow Boiling Characteristics in Microchannels

Iraqi Ministry of Higher Education and Scientific Researc

Flow boiling in microchannels with HFE-7100: Experimental results and comparison with correlations

An experimental investigation of flow boiling heat transfer in a copper multi-microchannel heat sink with a hydraulic diameter of 0.46 mm is described in this paper. The heat sink consisted of 25 rectangular microchannels, which were 0.7 mm wide, 0.35 mm deep and 25 mm long. The separating wall thickness between the channels was 0.1 mm and the width of the heat sink was 20 mm giving a base area of 500 mm2. HFE-7100 was chosen as the test fluid due to its environmentally friendly nature and high dielectric strength. It was also considered a suitable choice for cooling electronic components that require working surface temperature between 80 °C to 125 °C. A high-speed, high-resolution camera was used to capture the flow patterns during the experiments. All experiments were performed at a system pressure of 1 bar, inlet sub-cooling temperature of 5 K, mass flux ranging from 50 to 250 kg/m2 s and a heat flux range of 43.96−335.29 kW/m2. Four flow patterns were observed namely bubbly, slug, churn and annular flow. It was found that the local heat transfer coefficient increases with increasing heat flux and decreases slightly with increasing vapour quality, while there is a negligible effect of mass flux. The experimental results were compared with a number of existing heat transfer correlations that were proposed for macro and micro scale with some correlations showing good agreement. Similar comparisons with pressure drop correlations were also included

Multi-perspective evaluation of integrated active cooling systems using fuzzy decision making model

As global median temperatures continue to rise, the demand for active cooling systems (ACs) is increasing. These systems are particularly prevalent in developed countries for maintaining comfort during hot weather. Various ACs technologies are available, and assessing their performance in multi-perspective settings is necessary to determine the best option for intended usage. This requires an evaluation platform for assessment. This paper presents a novel multi-criteria decision-making (MCDM) model based on a new integrated 2-tuple linguistic Pythagorean fuzzy-weighted zero-inconsistency (2 TLP-FWZIC) and modified 2-tuple linguistic Pythagorean fuzzy multi-attributive border approximation area comparison (2TLPF-MABAC). The former is used to determine the importance of assessment criteria, while the latter is employed for selecting the optimal ACs using the obtained weights. The first-level weighting results reveal that performance criteria were predominantly favored for assessment, with ‘energy performance’ acquiring the most significant weight (0.2487) among all performance criteria. In terms of ACs selection results, among the 20 tested and assessed systems, the ‘geothermal borehole electricity-based ACs’ obtained the highest score value (0.1296), while the ‘window packaged electricity-based ACs’ had the lowest score (-0.0515). The robustness of the results was confirmed through sensitivity analysis

Averrhoa carambola leaves prevent dyslipidemia and oxidative stress in a rat model of poloxamer-407-induced acute hyperlipidemia

Background: The star fruit [Averrhoa carambola L (Oxalidaceae)] is traditionally used in the treatment of many ailments in many countries. It possesses several pharmacological activities, including antioxidant and anti-inflammatory effects. However, it contains the neurotoxic caramboxin and its high content of oxalic acid limits its consumption by individuals with compromised kidney function. This study assessed the anti-hyperlipidemic and antioxidant activities of different fractions of the methanolic extract of A. carambola leaves (MEACL). Methods: The antioxidant activity was investigated using FRAP, and ABTS and DPPH radical-scavenging assays and the inhibitory activity toward pancreatic lipase (PL) and HMG-CoA reductase was assayed in vitro. Acute hyperlipidemia was induced by poloxamer-407 (P-407) in rats and different fractions of MEACL (n-hexane, chloroform, n-butanol, ethyl acetate (EA), water, and chloroform) were orally administered. Cholesterol and triglycerides were determined at 0, 12, 24, and 48 h and LDL-C, vLDL-C, HDL-C, lipid peroxidation (LPO) and antioxidants were assayed after 48 h. The expression of ABCA1, ABCG5, ABCG8, LDL-R, SREBP-1, and SREBP-2 and the activity of HMG-CoA reductase were assayed in the liver of P-407-administered rats treated with the EA fraction. Results: The in vitro data revealed potent radical-scavenging activities of MEACL fractions with the most potent effect showed by the EA fraction that also suppressed the activities of HMG-CoA reductase and PL. In P-407-induced hyperlipidemic rats, all fractions prevented dyslipidemia as shown by the decrease in total cholesterol, triglycerides, LDL-C, vLDL-C and atherogenic index. MEACL and its fractions prevented LPO and boosted GSH, superoxide dismutase, glutathione peroxidase, and catalase in P-407-administered rats. The EA fraction showed more effective anti-hyperlipidemic and antioxidant effects than other fractions and downregulated SREBP-2 while upregulated ABCA1 and LDL-R and ameliorated LPL and HMG-CoA reductase in hyperlipidemic rats. Conclusion: MEACL showed in vitro and in vivo antioxidant activity and the EA fraction significantly ameliorated dyslipidemia in a rat model of P-407-induced acute hyperlipidemia by modulating LPL, PL, HMG-CoA reductase, and cholesterolgenesis-related factors. Therefore, the leaves of A. carambola represent a safe alternative for the star fruit particularly in kidney disease patients, and the EA is the most effective anti-hyperlipidemic and antioxidant fraction

Effect of myrrh and thyme on Trichinella spiralis enteral and parenteral phases with inducible nitric oxide expression in mice

Trichinellosis is a serious disease with no satisfactory treatment. We aimed to assess the effect of myrrh (Commiphora molmol

Branching on multi-aggregated variables

open5siopenGamrath, Gerald; Melchiori, Anna; Berthold, Timo; Gleixner, Ambros M.; Salvagnin, DomenicoGamrath, Gerald; Melchiori, Anna; Berthold, Timo; Gleixner, Ambros M.; Salvagnin, Domenic

Challenges and opportunities for the built environment in a carbon-constrained world for the next 100 years and beyond

Today, the built environment, including infrastructure for tunnels, bridges, highways, subways, railroads, harbors, buildings and airports, is responsible for a significant portion of the energy consumption, natural resource utilization, waste generation as well as CO2 and other environmentally harmful emissions in the United States and around the world. There is no silver bullet solution to achieve the ambitious goal of zero carbon buildings and a city infrastructure with significantly reduced CO2 emissions. Thus, multifaceted solutions should be developed. Another challenge associated with the built environment is aging and a large economic burden to upgrade and maintain the outdated infrastructure. The current status of the U.S. built environment is far below sustainable condition. Rapidly deteriorating infrastructure that must be replaced provides us with the unique opportunity to rethink where and how we should live in the future. In addition, current challenges related to economic and societal inequality in the United States and other global communities also force us to re-evaluate how humanity is connected and how we share resources for a sustainable and healthy future while keeping the Earth safe. The engineering solutions for our future built environment include, but are not limited to, the design and synthesis of new infrastructure materials with low carbon intensity, the development of new manufacturing options and technologies, and the integration of innovative functionalities into building envelopes