Genetic diversity and relationship analysis among accessions of Aegilops ssp in Turkey using amplified fragment length polymorphism (AFLP) markers

Amplified fragment length polymorphism (AFLP) DNA markers were used to assess the genetic diversity and relationships between 55 accessions of genus Aegilops, including the species Aegilops triuncialis L. (UUCC), Aegilops geniculata Roth (MMUU), Aegilops cylindrica Host (CCDD) and Aegilops umbellulata Zhuk (UU). The samples were collected from Aegean region and East Anatolia, Turkey. 16 AFLP selective primer combinations generated a total of 3200 polymorphic amplification products. 50 Aegilops accessions were analyzed using the data analysis software, unweighted pair-group method arithmetic average (UPGMA) method and numerical taxonomy and multivariate analysis system (NTSYSpc-2.02k). The similarity index coefficients were calculated according to simple matching coefficient. Using 16 AFLP primer combinations, species from Aegean region and east Anatolia were clustered as four major groups. Aegilops species having U genome clustered together and A. cylindrica host was out grouped

Dexamethasone and Basic‐Fibroblast Growth Factor Regulate Markers of Mineralization in Cementoblasts In Vitro

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142252/1/jper1550.pd

Arkeolojik DNA izolasyonu ve sekans analizi, modern örneklerle karşılaştırılması

Neolitik döneme ait kazı alanlarından elde edilen karbonlasın iş buğday örneklerinden DNA elde edilmiş ve çalışılmıştır. Çalışılan DNA, insanlığın yabanıl bir otu ıslah edip, ekmek yapmayı başardığı günümüzden 8500 yıl öncesine aittir ve dünyadaki en eski buğday DNA'^ıdır. Dolayısıyla, en eski ekmeklik buğday DNA sekansı, bu proje sayesinde bulunmuş, bu da Çatalhöyük halkının ekmeklik buğdayı üretip, tükettiklerinin moleküler düzeydeki ilk kanıtı olmuştur

Differential expression of genes possibly involved in incompatible interaction between barley and powdery mildew

Bu çalışma, 28 Eylül-01 Ekim 2011 tarihleri arasında İstanbul[Türkiye]’da düzenlenen European Biotechnology Congress’da bildiri olarak sunulmuştur.European Biotechnol Themat Network Asso

Mikrosatelit DNA molekül belirleyicileri kullanılarak bitki genotiplerinin tanımlanması

TÜBİTAK TBAG Proje28.02.1997Bu çalışmada BTD (Basit Tekrar Dizilim) DNA belirleyicileri kullanılarak 16 durum buğdayı kültivarında genetik ilişki tanımlanmış ve bu belirleyiciler ortalama 0.46 değerinde heterozigosite göstermişlerdir. Ayrıca, buğdayda BTD belrleyicileri izole etmek amacıyla BTD belrleyicileri için zenginleştirilmiş genom DNA klonları oluşturulmuştur

Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers

The winter type durum wheat varieties of Anatolia used in this study were differentiated for the first time by using simple sequence repeat (SSR) DNA markers or microsatellites. Seven microsatellite markers were used to distinguish four well-adapted landrace selections. five cultivars and seven recently obtained advancing lines. The loci of seven microsatellites were all homozygous, but the WMS6 locus occurred with two alleles in all the genotypes. The genotypes were all distinguished from each other, with the number of alleles ranging from live to 13. The lowest and highest polymorphism information content (PIC) values were observed to be 0.609 and 0.872, respectively. Three markers alone, WMS6, WMS30 and WMS120. can distinguish all 16 genotypes. UPGMA dendogram, based on a similarity matrix by a simple matching coefficient algorithm, is in accordance with the available pedigree information

Assessment of powdery mildew (Blumeria graminis f. sp. hordei) resistance genes in Turkish barley varieties

Thirty-four Turkish barley (Hordeum vulgare) varieties were tested for the presence of resistance genes (R-genes) to powdery mildew (Blumeria graminis f. sp. hordei) using the “leaf segment test” with nine isolates of the fungus. The most commonly found R-gene was Mla8, while a combination of R-genes Mlg and Ml(CP) was found in three varieties (Tokak 157/37, Beysehir 98, Konevei 98). The gene Mlh was found in four varieties (Obruk 86, Anadolu 86, Çıldır 02, Özdemir 05), while no R-gene was found in three varieties (Hamidiye 85, Yesevi 93, Bülbül 89). No clear inferences could be made for the partly-resistant varieties Gemici 7243, Yea 793.12 and Akhisar 98 using the test isolates selected, which suggests the presence of still unidentified R-genes. Overall, 10 previously characterized R-genes for powdery mildew (Mla8, Ml(La), Mlg, Ml(CP), Mlh, Mlat, Mla1, Mlh, Mla7, Mlra) were present in Turkish barley varieties, along with some other(s) still to be identified.Trente-quatre variétés d’orge (Hordeum vulgare) cultivées en Turquie ont été testées pour la présence de gènes de résistance (R) a l’oïdium (blanc) (Blumeria graminis f. sp. hordei) a l’aide du « test des segments foliaires » et de neuf isolats du champignon. Le gène R le plus commun parmi les variétés testées était Mla8, alors qu’une combinaison des gènes Mlg et Ml(CP) était trouvée chez trois variétés (Tokak 157/37, Beysehir 98, Konevei 98). La présence du gène Mlh a été identifiée chez quatre variétés (Obruk 86, Anadolu 86, Çıldır 02, Özdemir 05), alors qu’aucun gène R n’a été détecté dans trois autres variétés (Hamidiye 85, Yesevi 93, Bülbül 89). Aucune inférence n’a pu être clairement établie pour les variétés Gemici 7243, Yea 793.12 et Akhisar 98 a l’aide des isolats fongiques utilisés, ce qui suggère la présence de gènes R encore non identifiés. Au total, 10 gènes de résistance à l’oïdium (Mla8, Ml(La), Mlg, Ml(CP), Mlh, Mlat, Mla1, Mlh, Mla7, Mlra) étaient présents dans les variétés d’orge cultivées en Turquie, en plus d’autre(s) gène(s) non identifié(s)

Ancient DNA from 8400 Year-Old Çatalhöyük Wheat: Implications for the Origin of Neolithic Agriculture

Human history was transformed with the advent of agriculture in the Fertile Crescent with wheat as one of the founding crops. Although the Fertile Crescent is renowned as the center of wheat domestication, archaeological studies have shown the crucial involvement of Catalhoyuk in this process. This site first gained attention during the 1961-65 excavations due to the recovery of primitive hexaploid wheat. However, despite the seeds being well preserved, a detailed archaeobotanical description of the samples is missing. In this article, we report on the DNA isolation, amplification and sequencing of ancient DNA of charred wheat grains from Catalhoyuk and other Turkish archaeological sites and the comparison of these wheat grains with contemporary wheat species including T. monococcum, T. dicoccum, T. dicoccoides, T. durum and T. aestivum at HMW glutenin protein loci. These ancient samples represent the oldest wheat sample sequenced to date and the first ancient wheat sample from the Middle East. Remarkably, the sequence analysis of the short DNA fragments preserved in seeds that are approximately 8400 years old showed that the Catalhoyuk wheat stock contained hexaploid wheat, which is similar to contemporary hexaploid wheat species including both naked (T. aestivum) and hulled (T. spelta) wheat. This suggests an early transitory state of hexaploid wheat agriculture from the Fertile Crescent towards Europe spanning present-day Turkey.Publisher's Versio

GENETIC STRUCTURE OF POWDERY MILDEW DISEASE PATHOGEN BLUMERIA GRAMINIS F. SP HORDEI IN THE BARLEY FIELDS OF CUKUROVA IN TURKEY

WOS: 000395724300043This work was conducted in order to investigate the frequency of virulence genes, gene complexities, and pathotype frequencies of powdery mildew (Blumeria graminis f. sp. hordei) populations on two different barley production fields in the Cukurova Region. For this purpose, the barley leaves, prior to the pre-harvest period, which were infected with pathogen at the sexual period developmental stage as Cleistothecium were collected in 2007 and stored in the laboratory at room temperature. Pathogenic isolates were obtained in laboratory conditions with the aid of susceptible control variety (Bulbul-89) by stimulating ascospore output from each foliar. Every isolate was further purified through multiplying from a single spore. A total of 138 Blumeria graminis f. sp. hordei single spores were obtained. The differential isogenic lines of 25, each possessing a single resistance genes were inoculated with the single spore isolates obtained from Cleistothecium. The aggressiveness of gene frequencies and virulence gene complexities, and pathotype frequencies of the isolates were determined according to the scale 0-4, developed by Welz. The pathotypes were established through the formula developed by Habgood. Virulence gene frequencies varied from 0 % to 94.33 % in powdery mildew populations. Gene frequencies of Val, (Va7+Vk) were at 0.0 % in both populations. In the populations of Adana and Hatay 47 and 56 pathotypes were obtained, respectively. The isolate with Habgood pathotype index of 04667601 showed 14.8 % gene frequency in Adana population.DOSAP Program of METU; State Planning Organization, DPTTurkiye Cumhuriyeti Kalkinma Bakanligi [DPT2004K120750]We gratefully acknowledge Dr. Mogens S. Hovmoller and ICARDA for providing the Blumeria graminis f. sp. hordei races and Barley differential lines, respectively. We also thank to Adnan Menderes University for using their facilities. This work was funded by the DOSAP Program of METU and State Planning Organization, DPT (Project No. DPT2004K120750)