Motility fractionation of bacteria by centrifugation

Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.Comment: 7 pages, 5 figures (in press

Observations of the seasonality of the Antarctic microseismic signal, and its association to sea ice variability

International audienceSeismic noise spectra at all seismic stations display two peaks in the 1-20 s period band, called primary and secondary microseisms. They are caused by the coupling of ocean waves into Rayleigh waves. At most locations, microseismic power is greater during local winter (when nearby oceans are stormier) than local summer. This tendency is reversed for stations in Antarctica, where growth of local winter sea ice seems to impede microseism generation in near coastal areas. A decade of continuous data from coastal seismic stations in Antarctica show systematic seasonality in microseismic signal levels, and demonstrate associations with both broad‐ scale and local sea‐ice conditions. Primary microseisms are known to be generated at the coast and the modulation that we observe can be associated with sea‐ice variations both in the vicinity of the station and along other Antarctic coasts. The similar modulation of short‐period secondary microseisms corroborates their mostly near‐coastal origin, while the con- tinued presence of long‐period secondary microseisms sug- gests more distant source regions. These observations could be used to extend the monitoring of climate variability prior to the availability of satellite‐derived climate indicators

Generalized energy equipartition in harmonic oscillators driven by active baths

We study experimentally and numerically the dynamics of colloidal beads confined by a harmonic potential in a bath of swimming E. coli bacteria. The resulting dynamics is well approximated by a Langevin equation for an overdamped oscillator driven by the combination of a white thermal noise and an exponentially correlated active noise. This scenario leads to a simple generalization of the equipartition theorem resulting in the coexistence of two different effective temperatures that govern dynamics along the flat and the curved directions in the potential landscape.Comment: 4 pages, 3 figure

Seismic tomography of the southern California crust based on spectral-element and adjoint methods

We iteratively improve a 3-D tomographic model of the southern California crust using numerical simulations of seismic wave propagation based on a spectral-element method (SEM) in combination with an adjoint method. The initial 3-D model is provided by the Southern California Earthquake Center. The data set comprises three-component seismic waveforms (i.e. both body and surface waves), filtered over the period range 2–30 s, from 143 local earthquakes recorded by a network of 203 stations. Time windows for measurements are automatically selected by the FLEXWIN algorithm. The misfit function in the tomographic inversion is based on frequency-dependent multitaper traveltime differences. The gradient of the misfit function and related finite-frequency sensitivity kernels for each earthquake are computed using an adjoint technique. The kernels are combined using a source subspace projection method to compute a model update at each iteration of a gradient-based minimization algorithm. The inversion involved 16 iterations, which required 6800 wavefield simulations. The new crustal model, m_(16), is described in terms of independent shear (V_S) and bulk-sound (V_B) wave speed variations. It exhibits strong heterogeneity, including local changes of ±30 per cent with respect to the initial 3-D model. The model reveals several features that relate to geological observations, such as sedimentary basins, exhumed batholiths, and contrasting lithologies across faults. The quality of the new model is validated by quantifying waveform misfits of full-length seismograms from 91 earthquakes that were not used in the tomographic inversion. The new model provides more accurate synthetic seismograms that will benefit seismic hazard assessment

Characteristic atmosphere-ocean-solid earth interactions in the Antarctic coastal and marine environment inferred from seismic and infrasound recording at Syowa Station, East Antarctica

International audienceSeveral characteristic waves detected by seismographs in Antarctic stations have been recognized as originating from the physical interaction between the solid earth and the atmosphere-ocean-cryosphere system surrounding the Antarctic and may be used as a proxy for characterizing ocean wave climate. A Chaparral-type infrasound sensor was installed at Syowa Station (SYO; 39.6E, 69.0S), East Antarctica, in April 2008 during the International Polar Year (IPY2007-2008). Matching data are also available for this time period from the existing broadband seismic recorder located close by. Continuous infrasound data for 2008-2009 include background signals (microbaroms) with a broad peak in the wave period between the values of 4 and 10 s. Signals with the same period are recorded by the broadband seismograph at SYO (microseisms). This period band is identified as double-frequency microseisms/baroms (DFM). The DFM have relatively lower amplitudes during winter. We suggest that this is due to the sea-ice extent around the coast causing a decreased ocean loading effect. In contrast, the single frequency microseisms/baroms with a peak in period between 12 and 30 s are observed under storm conditions, particularly in winter. On the infrasound data, stationary signals are identified with harmonic overtones at a few Hertz to lowermost human audible band, which we suggest is due to local effects such as sea-ice cracking and vibration. Microseism measurements are a useful proxy for characterizing ocean wave climate, complementing other oceanographic and geophysical data. At SYO, continuous monitoring by both broadband seismograph and infrasound contributes to the Federation of Digital Seismographic Networks, the Comprehensive Nuclear-Test-Ban Treaty in the high southern latitudes and the Pan-Antarctic Observations System under the Scientific Committee on Antarctic Research

Recent seismicity on the Kerguelen islands

The Kerguelen archipelago, one of the largest oceanic archipelagos in the world, was built by an active hotspot interacting with a ridge between 110 and 40 million years ago; since then, the ridge has migrated over 1000~km away and the archipelago's volcanic activity has been steadily decreasing. Despite the lack of recent active tectonics and the quiescent volcanism of the Kerguelen archipelago, there have been several observations of seismic events of unknown origin in its vicinity. The only seismic instrument within 1000~km of the archipelago was installed on Kerguelen's main island in the 1980's. In this study we apply modern earthquake detection techniques to the continuous waveforms recorded by this seismometer over the past 20 years. We reveal that the Kerguelen archipelago islands hosts an abundant seismicity. This seismicity exhibits swarm-like characteristics in several clusters while at other locations the earthquakes appear more steady over time. We locate most events near the largest icecap of the main island. We speculate that the origin of the earthquakes can be linked to residual volcanic, magmatic, or hydrothermal activity at depth, all of which can be favored by flexural stress caused by the documented fast retreat of icecap. This seismicity may also indicate that the Kerguelen hotspot shows signs of unrest

Atmosphere - Ocean - Solid Earth Interaction from Microseisms & Microbaroms recorded at Syowa Station, East Antarctica

第33回極域気水圏シンポジウム11月30日（火） 国立極地研究所 ２階大会議

Rayleigh wave group velocities in North-West Iran: SOLA Backus-Gilbert vs. Fast Marching tomographic methods

In this study, we focus on Northwest Iran and exploit a dataset of Rayleigh-wave group-velocity measurements obtained from ambient noise cross-correlations and earthquakes. We build group-velocity maps using the recently developed SOLA Backus-Gilbert linear tomographic scheme as well as the more traditional Fast-marching Surface-wave Tomography method. The SOLA approach produces robust, unbiased local averages of group velocities with detailed information on their local resolution and uncertainty; however, it does not as yet allow ray-path updates in the inversion process. The Fast-marching method, on the other hand, does allow ray-path updates, although it does not provide information on the resolution and uncertainties of the resulting models (at least not without great computational cost) and may suffer from bias due to model regularisation. The core of this work consists in comparing these two tomographic methods, in particular how they perform in the case of strong vs. weak seismic-velocity contrasts and good vs. poor data coverage. We demonstrate that the only case in which the Fast-marching inversion outperforms the SOLA inversion is for strong anomaly contrasts in regions with good path coverage; in all other configurations, the SOLA inversion produces more coherent anomalies with fewer artefacts

An automated time-window selection algorithm for seismic tomography

We present FLEXWIN, an open source algorithm for the automated selection of time windows on pairs of observed and synthetic seismograms. The algorithm was designed specifically to accommodate synthetic seismograms produced from 3-D wavefield simulations, which capture complex phases that do not necessarily exist in 1-D simulations or traditional traveltime curves. Relying on signal processing tools and several user-tuned parameters, the algorithm is able to include these new phases and to maximize the number of measurements made on each seismic record, while avoiding seismic noise. Our motivation is to use the algorithm for iterative tomographic inversions, in which the synthetic seismograms change from one iteration to the next. Hence, automation is needed to handle the volume of measurements and to allow for an increasing number of windows at each model iteration. The algorithm is sufficiently flexible to be adapted to many tomographic applications and seismological scenarios, including those based on synthetics generated from 1-D models. We illustrate the algorithm using data sets from three distinct regions: the entire globe, the Japan subduction zone, and southern California

Synthesis of Imidazolidin-2-ones and Imidazol-2-ones via Base-Catalyzed Intramolecular Hydroamidation of Propargylic Ureas under Ambient Conditions

The first organo-catalyzed synthesis of imidazolidin-2-ones and imidazol-2-ones via intramolecular hydroamidation of propargylic ureas is reported. The phosphazene base BEMP turned out to be the most active organo-catalyst compared with guanidine and amidine bases. Excellent chemo- and regioselectivities to five-membered cyclic ureas have been achieved under ambient conditions, with a wide substrate scope and exceptionally short reaction times (down to 1 min). A base-mediated isomerization step to an allenamide intermediate is the most feasible reaction pathway to give imidazol-2-ones, as suggested by DFT studies