Repeat Organ Transplantation in the United States, 1996–2005

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72698/1/j.1600-6143.2007.01786.x.pd

Patient Selection and Volume in the Era Surrounding Implementation of Medicare Conditions of Participation for Transplant Programs

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110882/1/hesr12188.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/110882/2/hesr12188-sup-0001-AuthorMatrix.pd

Implementation of Vascularized Composite Allografts in the United States: Recommendations From the ASTS VCA Ad Hoc Committee and the Executive Committee

Like all other areas of transplantation, vascularized composite allografts (VCA) has the capacity to transform the lives of patients, for the better or for the worse. It is this duality that mandates VCA be performed in centers prepared for the intricacies accompanying other transplant procedures. Similarly, the complexities of VCA require that the procedures be driven by surgeons and physicians with experience in the multidisciplinary management of immunocompromised postsurgical patients. Furthermore, the grafts should be considered as organs rather than tissues from a regulatory and a biological standpoint. The ASTS supports the field of VCA and has demonstrated its support and leadership by actively formulating a strategy for its systematic development. The goal of this document is to provide a framework for the prospective, thoughtful realization of VCA in the United States from the American Society of Transplant Surgeons (ASTS) perspective.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/79113/1/j.1600-6143.2010.03374.x.pd

Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies

Abstract Background Assisted reproductive technologies (ART) are widely used to treat fertility issues in humans and for the production of embryos in mammalian livestock. The use of these techniques, however, is not without consequence as they are often associated with inauspicious pre- and postnatal outcomes including premature birth, intrauterine growth restriction and increased incidence of epigenetic disorders in human and large offspring syndrome in cattle. Here, global DNA methylation profiles in the trophectoderm and embryonic discs of in vitro produced (IVP), superovulation-derived (SOV) and unstimulated, synchronised control day 17 bovine conceptuses (herein referred to as AI) were interrogated using the EmbryoGENE DNA Methylation Array (EDMA). Pyrosequencing was used to validate four loci identified as differentially methylated on the array and to assess the differentially methylated regions (DMRs) of six imprinted genes in these conceptuses. The impact of embryo-production induced DNA methylation aberrations was determined using Ingenuity Pathway Analysis, shedding light on the potential functional consequences of these differences. Results Of the total number of differentially methylated loci identified (3140) 77.3 and 22.7% were attributable to SOV and IVP, respectively. Differential methylation was most prominent at intragenic sequences within the trophectoderm of IVP and SOV-derived conceptuses, almost a third (30.8%) of the differentially methylated loci mapped to intragenic regions. Very few differentially methylated loci were detected in embryonic discs (ED); 0.16 and 4.9% of the differentially methylated loci were located in the ED of SOV-derived and IVP conceptuses, respectively. The overall effects of SOV and IVP on the direction of methylation changes were associated with increased methylation; 70.6% of the differentially methylated loci in SOV-derived conceptuses and 57.9% of the loci in IVP-derived conceptuses were more methylated compared to AI-conceptuses. Ontology analysis of probes associated with intragenic sequences suggests enrichment for terms associated with cancer, cell morphology and growth. Conclusion By examining (1) the effects of superovulation and (2) the effects of an in vitro system (oocyte maturation, fertilisation and embryo culture) we have identified that the assisted reproduction process of superovulation alone has the largest impact on the DNA methylome of subsequent embryos

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a Delta9 desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibioticinsensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the Delta9 desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transformation to engineer lipid metabolic pathways in both vegetative and reproductive tissues and suggest an increase of cold tolerance in transplastomic plants showing altered leaf fatty acid profiles. This is the first example of transplastomic plants expressing an agronomically relevant gene produced with the ‘‘binding-type’’ vectors, which do not contain a heterologous marker gene. In fact, the transplastomic plants expressing the S. commersonii gene contain only plant-derived sequences, a clear attraction from a public acceptability perspective

A self-consistent method to estimate the rate of compact binary coalescences with a Poisson mixture model

The recently published GWTC-1 (Abbott B P et al (LIGO Scientific Collaboration and Virgo Collaboration) 2019 Phys. Rev. X 9 031040)—a journal article summarizing the search for gravitational waves (GWs) from coalescing compact binaries in data produced by the LIGO-Virgo network of ground-based detectors during their first and second observing runs—quoted estimates for the rates of binary neutron star, neutron star black hole binary, and binary black hole mergers, as well as assigned probabilities of astrophysical origin for various significant and marginal GW candidate events. In this paper, we delineate the formalism used to compute these rates and probabilities, which assumes that triggers above a low ranking statistic threshold, whether of terrestrial or astrophysical origin, occur as independent Poisson processes. In particular, we include an arbitrary number of astrophysical categories by redistributing, via mass-based template weighting, the foreground probabilities of candidate events, across source classes. We evaluate this formalism on synthetic GW data, and demonstrate that this method works well for the kind of GW signals observed during the first and second observing runs

A self-consistent method to estimate the rate of compact binary coalescences with a Poisson mixture model

The recently published GWTC-1 - a journal article summarizing the search for gravitational waves (GWs) from coalescing compact binaries in data produced by the LIGO-Virgo network of ground-based detectors during their first and second observing runs - quoted estimates for the rates of binary neutron star, neutron star black hole binary, and binary black hole mergers, as well as assigned probabilities of astrophysical origin for various significant and marginal GW candidate events. In this paper, we delineate the formalism used to compute these rates and probabilities, which assumes that triggers above a low ranking statistic threshold, whether of terrestrial or astrophysical origin, occur as independent Poisson processes. In particular, we include an arbitrary number of astrophysical categories by redistributing, via mass-based template weighting, the foreground probabilities of candidate events, across source classes. We evaluate this formalism on synthetic GW data, and demonstrate that this method works well for the kind of GW signals observed during the first and second observing runs.Comment: 19 pages, 5 figure

A self-consistent method to estimate the rate of compact binary coalescences with a Poisson mixture model

The recently published GWTC-1 - a journal article summarizing the search for gravitational waves (GWs) from coalescing compact binaries in data produced by the LIGO-Virgo network of ground-based detectors during their first and second observing runs - quoted estimates for the rates of binary neutron star, neutron star black hole binary, and binary black hole mergers, as well as assigned probabilities of astrophysical origin for various significant and marginal GW candidate events. In this paper, we delineate the formalism used to compute these rates and probabilities, which assumes that triggers above a low ranking statistic threshold, whether of terrestrial or astrophysical origin, occur as independent Poisson processes. In particular, we include an arbitrary number of astrophysical categories by redistributing, via mass-based template weighting, the foreground probabilities of candidate events, across source classes. We evaluate this formalism on synthetic GW data, and demonstrate that this method works well for the kind of GW signals observed during the first and second observing runs.Comment: 19 pages, 5 figure

Persistence of anticancer activity in berry extracts after simulated gastrointestinal digestion and colonic fermentation

Fruit and vegetable consumption is associated at the population level with a protective effect against colorectal cancer. Phenolic compounds, especially abundant in berries, are of interest due to their putative anticancer activity. After consumption, however, phenolic compounds are subject to digestive conditions within the gastrointestinal tract that alter their structures and potentially their function. However, the majority of phenolic compounds are not efficiently absorbed in the small intestine and a substantial portion pass into the colon. We characterized berry extracts (raspberries, strawberries, blackcurrants) produced by in vitro-simulated upper intestinal tract digestion and subsequent fecal fermentation. These extracts and selected individual colonic metabolites were then evaluated for their putative anticancer activities using in vitro models of colorectal cancer, representing the key stages of initiation, promotion and invasion. Over a physiologically-relevant dose range (0–50 µg/ml gallic acid equivalents), the digested and fermented extracts demonstrated significant anti-genotoxic, anti-mutagenic and anti-invasive activity on colonocytes. This work indicates that phenolic compounds from berries undergo considerable structural modifications during their passage through the gastrointestinal tract but their breakdown products and metabolites retain biological activity and can modulate cellular processes associated with colon cancer