Tecnologia assistiva cães-guia no Brasil: uma ação política orientada à inclusão social de pessoas com deficiência visual

This article aims to present the experience of implementation and operation of the Training Centre of Trainers and Instructors of guide dogs at the Catarinense Federal Institute – Campus Camboriú. In order to achieve the proposed, we contextualize and analyze how was established the government policy that aim at social inclusion of people with disabilities and we discussed Guide dogs’ Assistive Technology, an alternative supporting mode for people with visual impairment, highlighting its impact on the lives of users.Este artículo tiene como objetivo presentar la experiencia de implementación y operación del Centro de capacitación para instructores e instructores de perros guía en el Instituto Federal de Santa Catarina - Campus Camboriú. Para lograr la propuesta, contextualizamos y analizamos cómo se estableció la política del gobierno dirigida a la inclusión social de las personas con discapacidad y discutimos la Assistive Technology Guide Dogs, una movilidad alternativa que apoya a las personas con discapacidad visual, destacando su impacto en la vida. de los usuarios.Este artigo possui como propósito apresentar a experiência de implantação e funcionamento do Centro de Formação de Treinadores e Instrutores de Cães-Guia no Instituto Federal Catarinense – Campus Camboriú. Para alcançar o proposto, contextualizamos e analisamos o modo como se estabeleceu a política governamental que visou a inclusão social de pessoas com deficiência e discutimos a Tecnologia Assistiva Cães-Guia, uma mobilidade alternativa de apoio a pessoas com deficiência visual, destacando seu impacto na vida dos usuários

Dry matter accumulation and carbohydrate composition of a new cultivar of maize, "Sugary-Opaque-2-Waxy".

O acúmulo de matéria seca e a composição de carboidratos de uma nova cultivar de milho, o triplo mutante Sugary-Opaque-2-Waxy, foram estudados comparativamente em relação às cultivares Sugary-Opaque-2, Waxy-Sugary-Opaque-2 e uma cultivar de milho (Zea mays L.) normal. De uma maneira geral, todas as cvs. estudadas apresentaram, aos 25 dias após a polinização (DAP) teores semelhantes de açúcares solúveis redutores. As cultivares que continham o gene sugary apresentaram teores mais elevados de acúcares solúveis totais e de fitoglicogênio, em comparação com as cultivares com endospernia amiláceo. Uma relação inversa foi observada para o conteúdo de amido. A avaliação do acúmulo de matéria seca, em diferentes estádios de maturação, 15, 20, 25, 30 e 35 DAP, indicou que o milho normal e o triplo mutante apresentaram os maiores e menores valores de porcentagem de peso seco, respectivamente. O triplo mutante mostrou acúmulo de matéria seca mais lento do que seus ascendentes, proporcionando um período mais prolongado de colheita como milho verde, favorecendo o seu consumo in naturaDry matter accumulation and carbohydrate composition of a new cultivar of maize (Zea mays L.), te triple mutant Sugary-Opaque-2-Waxy, was comparatively studied in relation to the cultivars Sugary, Opaque-2, Waxy,Sugary-Opaque-2 and Maya Normal in general, all cultivars showed similar content of soluble reducing sugars. The cultivars with te sugary gene showed higher content of total soluble sugars and phytoglycogen than those with the starchy endosperm. The opposite was found to te starch content Maya Normal and te triple mutant showed, respectively, te higher and lower values for dry matter accumulation at the different stages of endosperm development (15, 20, 25, 30 and 35 days after poilination - DAP). The lower rate of dry matter accumulation suggests that te triple mutant would be more appropriate to be consumed as sweet corn

Soil effects on stem growth and wood anatomy of tamboril are mediated by tree age

Soil influences the growth of trees and the characteristics of the wood; but could this influence change during the ontogeny of trees? To answer this question, we analyzed the wood anatomy of 9-year-old trees and 2-year-old saplings of Enterolobium contortisiliquum, known as “tamboril”, growing in eutrophic and oligotrophic soil in the Brazilian Cerrado, and assessed the effect of age on plant–soil relationship. Sapwood samples were collected from the main stem, anatomical sections were prepared in the lab, and 12 anatomical wood traits were measured. Individuals in eutrophic soil had greater stem diameter and height than those in oligotrophic soil. Trees in eutrophic soil had vessel-associated parenchyma cells with abundant storage compounds. Fibers walls were 47% thicker and intervessel pits diameter were 14% larger in trees of eutrophic soil. A greater proportion of solitary vessels (74%) was observed in trees rather than in saplings (50%). The secondary xylem of trees was mainly formed by fibers (63%) whereas in saplings it was mainly formed by storage tissue (64%). Our study provides evidence that the influence of soil conditions on tree growth reflects variations in wood anatomical features. No significant response to soil type was observed in saplings, thus demonstrating that the relationship between soil type and wood growth is mediated by tree age. These findings help to develop reliable reforestation strategies in tropical ecosystems characterized by different levels of soil fertility

Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection

<p>Abstract</p> <p>Background</p> <p>Successful achievement of early folliculogenesis is crucial for female reproductive function. The process is finely regulated by cell-cell interactions and by the coordinated expression of genes in both the oocyte and in granulosa cells. Despite many studies, little is known about the cell-specific gene expression driving early folliculogenesis. The very small size of these follicles and the mixture of types of follicles within the developing ovary make the experimental study of isolated follicular components very difficult.</p> <p>The recently developed laser capture microdissection (LCM) technique coupled with microarray experiments is a promising way to address the molecular profile of pure cell populations. However, one main challenge was to preserve the RNA quality during the isolation of single cells or groups of cells and also to obtain sufficient amounts of RNA.</p> <p>Using a new LCM method, we describe here the separate expression profiles of oocytes and follicular cells during the first stages of sheep folliculogenesis.</p> <p>Results</p> <p>We developed a new tissue fixation protocol ensuring efficient single cell capture and RNA integrity during the microdissection procedure. Enrichment in specific cell types was controlled by qRT-PCR analysis of known genes: six oocyte-specific genes (<it>SOHLH2</it>, <it>MAEL</it>, <it>MATER</it>, <it>VASA</it>, <it>GDF9</it>, <it>BMP15</it>) and three granulosa cell-specific genes (<it>KL</it>, <it>GATA4</it>, <it>AMH</it>).</p> <p>A global gene expression profile for each follicular compartment during early developmental stages was identified here for the first time, using a bovine Affymetrix chip. Most notably, the granulosa cell dataset is unique to date. The comparison of oocyte vs. follicular cell transcriptomes revealed 1050 transcripts specific to the granulosa cell and 759 specific to the oocyte.</p> <p>Functional analyses allowed the characterization of the three main cellular events involved in early folliculogenesis and confirmed the relevance and potential of LCM-derived RNA.</p> <p>Conclusions</p> <p>The ovary is a complex mixture of different cell types. Distinct cell populations need therefore to be analyzed for a better understanding of their potential interactions. LCM and microarray analysis allowed us to identify novel gene expression patterns in follicular cells at different stages and in oocyte populations.</p

Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition

A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world's planktonic ecosystems