21 research outputs found
The Birth of DEFA Genre Cinema, East German Sci-fi Films, New Technologies, and Coproduction with Eastern Europe: Der schweigende Stern (Silent Star, Kurt Maetzig, 1960)
A Lentiviral Fluorescent Genetic Barcoding System for Flow Cytometry-Based Multiplex Tracking
MicroRNA-150-regulated vectors allow lymphocyte-sparing transgene expression in hematopoietic gene therapy
GTPase domain driven dimerization of SEPT7 is dispensable for the critical role of septins in fibroblast cytokinesis
Activation of Evi1 inhibits cell cycle progression and differentiation of hematopoietic progenitor cells
Counting stem cells:methodological constraints
The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies