177 research outputs found
Polyandrous females avoid costs of inbreeding
Why do females typically mate with more than one male? Female mating patterns have broad implications for sexual selection, speciation and conflicts of interest between the sexes, and yet they are poorly understood. Matings inevitably have costs, and for females, the benefits of taking more than one mate are rarely obvious. One possible explanation is that females gain benefits because they can avoid using sperm from genetically incompatible males, or invest less in the offspring of such males. It has been shown that mating with more than one male can increase offspring viability, but we present the first clear demonstration that this occurs because females with several mates avoid the negative effects of genetic incompatibility. We show that in crickets, the eggs of females that mate only with siblings have decreased hatching success. However, if females mate with both a sibling and a non-sibling they avoid altogether the low egg viability associated with sibling matings. If similar effects occur in other species, inbreeding avoidance may be important in understanding the prevalence of multiple mating
Experimental Infection of Conventional Pigs with Streptococcus suis serotype 2 by Aerosolic Exposure
No Detectable Fertility Benefit from a Single Additional Mating in Wild Stalk-Eyed Flies
Background: Multiple mating by female insects is widespread, and the explanation(s) for repeated mating by females has been the subject of much discussion. Females may profit from mating multiply through direct material benefits that increase their own reproductive output, or indirect genetic benefits that increase offspring fitness. One particular direct benefit that has attracted significant attention is that of fertility assurance, as females often need to mate multiply to achieve high fertility. This hypothesis has never been tested in a wild insect population.Methodology/Principal Findings: Female Malaysian stalk-eyed flies (Teleopsis dalmanni) mate repeatedly during their lifetime, and have been shown to be sperm limited under both laboratory and field conditions. Here we ask whether receiving an additional mating alleviates sperm limitation in wild females. In our experiment one group of females received a single additional mating, while a control group received an interrupted, and therefore unsuccessful, mating. Females that received an additional mating did not lay more fertilised eggs in total, nor did they lay proportionately more fertilised eggs. Female fertility declined significantly through time, demonstrating that females were sperm limited. However, receipt of an additional mating did not significantly alter the rate of this decline.Conclusions/Significance: Our data suggest that the fertility consequences of a single additional mating were small. We discuss this effect (or lack thereof), and suggest that it is likely to be attributed to small ejaculate size, a high proportion of failed copulations, and the presence of X-linked meiotic drive in this species
A rapidly spinning supermassive black hole at the centre of NGC 1365
Broad X-ray emission lines from neutral and partially ionized iron observed in active galaxies have been interpreted as fluorescence produced by the reflection of hard X-rays off the inner edge of an accretion disk. In this model, line broadening and distortion result from rapid rotation and relativistic effects near the black hole, the line shape being sensitive to its spin. Alternative models in which the distortions result from absorption by intervening structures provide an equally good description of the data, and there has been no general agreement on which is correct. Recent claims that the black hole (2 × 10^6 solar masses) at the centre of the galaxy NGC 1365 is rotating at close to its maximum possible speed rest on the assumption of relativistic reflection. Here we report X-ray observations of NGC 1365 that reveal the relativistic disk features through broadened Fe-line emission and an associated Compton scattering excess of 10–30 kiloelectronvolts. Using temporal and spectral analyses, we disentangle continuum changes due to time-variable absorption from reflection, which we find arises from a region within 2.5 gravitational radii of the rapidly spinning black hole. Absorption-dominated models that do not include relativistic disk reflection can be ruled out both statistically and on physical grounds
NuSTAR and XMM-Newton observations of NGC 1365: Extreme absorption variability and a constant inner accretion disk
We present a spectral analysis of four coordinated NuSTAR+XMM-Newton
observations of the Seyfert galaxy NGC 1365. These exhibit an extreme level of
spectral variability, which is primarily due to variable line-of-sight
absorption, revealing relatively unobscured states in this source for the first
time. Despite the diverse range of absorption states, each of the observations
displays the same characteristic signatures of relativistic reflection from the
inner accretion disk. Through time-resolved spectroscopy we find that the
strength of the relativistic iron line and the Compton reflection hump relative
to the intrinsic continuum are well correlated, as expected if they are two
aspects of the same broadband reflection spectrum. We apply self-consistent
disk reflection models to these time-resolved spectra in order to constrain the
inner disk parameters, allowing for variable, partially covering absorption to
account for the vastly different absorption states observed. Each of the four
observations is treated independently to test the consistency of the results
obtained for the black hole spin and the disk inclination, which should not
vary on observable timescales. We find both the spin and the inclination
determined from the reflection spectrum to be consistent, confirming NGC 1365
hosts a rapidly rotating black hole; in all cases the dimensionless spin
parameter is constrained to be a* > 0.97 (at 90% statistical confidence or
better)
Hearing Sensation Levels of Emitted Biosonar Clicks in an Echolocating Atlantic Bottlenose Dolphin
Emitted biosonar clicks and auditory evoked potential (AEP) responses triggered by the clicks were synchronously recorded during echolocation in an Atlantic bottlenose dolphin (Tursiops truncatus) trained to wear suction-cup EEG electrodes and to detect targets by echolocation. Three targets with target strengths of −34, −28, and −22 dB were used at distances of 2 to 6.5 m for each target. The AEP responses were sorted according to the corresponding emitted click source levels in 5-dB bins and averaged within each bin to extract biosonar click-related AEPs from noise. The AEP amplitudes were measured peak-to-peak and plotted as a function of click source levels for each target type, distance, and target-present or target-absent condition. Hearing sensation levels of the biosonar clicks were evaluated by comparing the functions of the biosonar click-related AEP amplitude-versus-click source level to a function of external (in free field) click-related AEP amplitude-versus-click sound pressure level. The results indicated that the dolphin's hearing sensation levels to her own biosonar clicks were equal to that of external clicks with sound pressure levels 16 to 36 dB lower than the biosonar click source levels, varying with target type, distance, and condition. These data may be assumed to indicate that the bottlenose dolphin possesses effective protection mechanisms to isolate the self-produced intense biosonar beam from the animal's ears during echolocation
Somatic PIK3R1 Variation as a Cause of Vascular Malformations and Overgrowth
PurposeSomatic activating variants in the PI3K-AKT pathway cause vascular malformations with and without overgrowth. We previously reported an individual with capillary and lymphatic malformation harboring a pathogenic somatic variant in PIK3R1, which encodes three PI3K complex regulatory subunits. Here, we investigate PIK3R1 in a large cohort with vascular anomalies and identify an additional 16 individuals with somatic mosaic variants in PIK3R1.MethodsAffected tissue from individuals with vascular lesions and overgrowth recruited from a multisite collaborative network was studied. Next-generation sequencing targeting coding regions of cell-signaling and cancer-associated genes was performed followed by assessment of variant pathogenicity.ResultsThe phenotypic and variant spectrum associated with somatic variation in PIK3R1 is reported herein. Variants occurred in the inter-SH2 or N-terminal SH2 domains of all three PIK3R1 protein products. Phenotypic features overlapped those of the PIK3CA-related overgrowth spectrum (PROS). These overlapping features included mixed vascular malformations, sandal toe gap deformity with macrodactyly, lymphatic malformations, venous ectasias, and overgrowth of soft tissue or bone.ConclusionSomatic PIK3R1 variants sharing attributes with cancer-associated variants cause complex vascular malformations and overgrowth. The PIK3R1-associated phenotypic spectrum overlaps with PROS. These data extend understanding of the diverse phenotypic spectrum attributable to genetic variation in the PI3K-AKT pathway
Placebo-controlled clinical trials: how trial documents justify the use of randomisation and placebo
Coherent methods in the X-ray sciences
X-ray sources are developing rapidly and their coherent output is growing
extremely rapidly. The increased coherent flux from modern X-ray sources is
being matched with an associated rapid development in experimental methods.
This article reviews the literature describing the ideas that utilise the
increased brilliance from modern X-ray sources. It explores how ideas in
coherent X-ray science are leading to developments in other areas, and vice
versa. The article describes measurements of coherence properties and uses this
discussion as a base from which to describe partially-coherent diffraction and
X-ray phase contrast imaging, with its applications in materials science,
engineering and medicine. Coherent diffraction imaging methods are reviewed
along with associated experiments in materials science. Proposals for
experiments to be performed with the new X-ray free-electron-lasers are briefly
discussed. The literature on X-ray photon correlation spectroscopy is described
and the features it has in common with other coherent X-ray methods are
identified. Many of the ideas used in the coherent X-ray literature have their
origins in the optical and electron communities and these connections are
explored. A review of the areas in which ideas from coherent X-ray methods are
contributing to methods for the neutron, electron and optical communities is
presented.Comment: A review articel accepted by Advances in Physics. 158 pages, 29
figures, 3 table
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