214 research outputs found

    Assembly of Saccharomyces cerevisiae 60S ribosomal subunits: role of factors required for 27S pre‐rRNA processing

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/102169/1/embj2011338-sup-0001.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102169/2/embj2011338.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102169/3/embj2011338-sup-0002.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102169/4/embj2011338-reviewer_comments.pd

    Early events of Bacillus anthracis germination identified by time-course quantitative proteomics

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    Germination of Bacillus anthracis spores involves rehydration of the spore interior and rapid degradation of several of the protective layers, including the spore coat. Here, we examine the temporal changes that occur during B. anthracis spore germination using an isobaric tagging system. Over the course of 17 min from the onset of germination, the levels of at least 19 spore proteins significantly decrease. Included are acid-soluble proteins, several known and predicted coat proteins, and proteins of unknown function. Over half of these proteins are small (less than 100 amino acids) and would have been undetectable by conventional gel-based analysis. We also identified 20 proteins, whose levels modestly increased at the later time points when metabolism has likely resumed. Taken together, our data show that isobaric labeling of complex mixtures is particularly effective for temporal studies. Furthermore, we describe a rigorous statistical approach to define relevant changes that takes into account the nature of data obtained from multidimensional protein identification technology coupled with the use of isobaric tags. This study provides an expanded list of the proteins that may be involved in germination of the B. anthracis spore and their relative levels during germination.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/55849/1/5199_ftp.pd

    Effect of growth-promoting technologies on Longissimus lumborum muscle fiber morphometrics, collagen solubility, and cooked meat tenderness

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    Citation: Ebarb, S. M., Drouillard, J. S., Maddock-Carlin, K. R., Phelps, K. J., Vaughn, M. A., Burnett, D. D., . . . Gonzalez, J. M. (2016). Effect of growth-promoting technologies on Longissimus lumborum muscle fiber morphometrics, collagen solubility, and cooked meat tenderness. Journal of Animal Science, 94(2), 869-881. doi:10.2527/jas2015-9888The objective of the study was to examine the effect of growth-promoting technologies (GP) on Longissimus lumborum steak tenderness, muscle fiber cross-sectional area (CSA), and collagen solubility. Crossbred feedlot heifers (n = 33; initial BW 464 +/- 6 kg) were blocked by BW and assigned to 1 of 3 treatments: no GP (CON; n = 11); implant, no zilpaterol hydrochloride (IMP; n = 11); implant and zilpaterol hydrochloride (COMBO; n = 11). Heifers assigned to receive an implant were administered Component TE-200 on d 0 of the study, and the COMBO group received 8.3 mg/kg DM of zilpaterol hydrochloride for the final 21 d of feeding with a 3 d withdrawal period. Following harvest, strip loins were collected and fabricated into 4 roasts and aged for 3, 14, 21, or 35 d postmortem. Fiber type was determined by immunohistochemistry. After aging, objective tenderness and collagen solubility were measured. There was a treatment x day of aging (DOA) interaction for Warner-Bratzler shear force (WBSF; P 0.31). Soluble collagen amount tended to be affected (P = 0.06) by a treatment x DOA interaction which was due to COMBO muscle having more soluble collagen than the other 2 treatments on d 21 of aging (P < 0.02). Correlation analysis indicated that type I, IIA, and IIX fiber CSA are positively correlated with WBSF at d 3 and 14 of aging (P < 0.01), but only type IIX fibers are correlated at d 21 and 35 of aging (P < 0.03). At these time periods, total and insoluble collagen became positively correlated with WBSF (P < 0.01). This would indicate that relationship between muscle fiber CSA and WBSF decreases during postmortem aging, while the association between WBSF and collagen characteristics strengthens. The use of GP negatively impacted meat tenderness primarily through increased muscle fiber CSA and not through altering collagen solubility

    Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks

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    Citation: Ebarb, S. M., Phelps, K. J., Drouillard, J. S., Maddock-Carlin, K. R., Vaughn, M. A., Burnett, D. D., . . . Gonzalez, J. M. (2017). Effects of anabolic implants and ractopamine-HCl on muscle fiber morphometrics, collagen solubility, and tenderness of beef longissimus lumborum steaks. Journal of Animal Science, 95(3), 1219-1231. doi:10.2527/jas2016.1263The objective of this study was to examine the effects of growth-promoting technologies (GP) and postmortem aging on longissimus lumborum muscle fiber cross-sectional area (CSA), collagen solubility, and their relationship to meat tenderness. Two groups of black-hided crossbred feedlot heifers (group 1: n = 33, initial BW 430 +/- 7 kg; group 2: n = 32, initial BW 466 +/- 7 kg) were blocked by BW and assigned to 1 of 3 treatments consisting of: no implant and no ractopamine hydrochloride (CON; n = 21); implant, no ractopamine hydrochloride (IMP; n = 22); implant and ractopamine hydrochloride (COMBO; n = 22). Heifers that received an implant were administered an implant containing 200 mg trenbolone acetate and 20 mg estradiol on d 0 of the study, and heifers in the COMBO group received 400 mg.head(-1).d(-1) of ractopamine hydrochloride for 28 (Group 1) or 29 d (Group 2) at the end of 90-(Group 1) or 106-d (Group 2) feeding period. Following harvest, strip loins were collected and further fabricated into 5 roasts for postmortem aging (DOA) periods of 2, 7, 14, 21, or 35 d. After aging, Warner-Bratzler shear force (WBSF), muscle fiber CSA, and collagen solubility were measured. There was no treatment x DOA interaction for WBSF (P = 0.86), but treatment and DOA impacted WBSF (P 0.33). Collagen amounts were not impacted by GP treatment (P > 0.72), but DOA increased the concentration of soluble collagen (P = 0.04). Fiber CSA of all fiber types were positively correlated (P < 0.05; r = 0.21 to 0.28) with WBSF only on d 2 of aging, while soluble collagen amount tended to negatively correlate with WBSF on d 7 and 14 of aging (P < 0.10; r = -0.24 and -0.23, respectively). Administration of GP during heifer finishing resulted in greater steak WBSF over 35 d of aging, which was not due to collagen characteristics and only minimally affected by fiber CSA

    Variação espacial de sulfetos voláteis acidificados e biodisponibilidade de metais extraídos simultaneamente em sedimentos ao norte da Baía de Todos os Santos, Bahia

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    Cadernos de Geociências, v. 13, n. 1 - Especial UFBA 70 anos, Jul. 2016.Original publicado em Cadernos de Geociências, v. 11, n. 1-2, Maio-Nov. 2014

    VARIAÇÃO ESPACIAL DE SULFETOS VOLÁTEIS ACIDIFICADOS E BIODISPONIBILIDADE DE METAIS EXTRAÍDOS SIMULTANEAMENTE EM SEDIMENTOS AO NORTE DA BAÍA DE TODOS OS SANTOS, BAHIA.

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    A distribuição espacial de sulfetos volatilizáveis em ácido (SVA) e metais (Cd, Cu, Pb, Ni e Zn) extraídos simultaneamente (MES) no substrato lamoso do sedimento de manguezal na porção norte da Baía de Todos os Santos (Bahia-Brasil) foram estudados em oito estações sob possível efeito urbano, industrial e portuário. O SVA foi extraído do sedimento anaeróbio com ácido clorídrico 1 mol L-1, a frio, enquanto os metais extraídos simultaneamente (MES) bivalentes são liberados. Não houve diferença estatística entre as estações. A razão MES/SVA, correlacionaram-se com as frações granulométricas, matéria orgânica (3.5%), carbono orgânico total (2.4%), nitrogênio total (0.25%), metais (mg Kg-1): Cd, Cu, Pb, Ni e Zn.  Comparando-se com valores de referência internacionais estão abaixo dos limites estabelecidos, mesmo em outras regiões do Brasil. Os valores < 1 para a relação [MES]/[SVA], sugere a não disponibilidade desses metais, o que indicam a ausência de efeitos adversos à biota
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