241 research outputs found
Economic Evaluation of Organic and Inorganic Resources for Recapitalizing Soil Fertility in Smallholder Maize-based Cropping Systems of Central Kenya
Structural adjustments programs (SAPs) in the last two decades have eliminated all farm-support programs leading to low usage of fertilizers by Kenyan smallholders. One way of addressing this problem is use of organic nutrient resources. This paper examines their cost-effectiveness as capital investments in replenishment of Nitrogen (N), Phosphorus (P), Potassium (K) and soil organic matter (SOM) in smallholder, Maize-based cropping systems. On-farm trials were established in Maragwa and Kirinyaga Districts in 2003/04. Maize was planted in 3 replicates in randomised complete block design (RCBD) using different levels of organic and inorganic fertilizer resources. A blanket rate of 40kg P/ha was applied in all treatment except the control to increase organic N-utilization efficiency. The test crop was harvested, oven-dried and weighed. Net Present Values (NPV) were computed using Partial Budgeting Analysis Model. Increasing levels of inorganic N increased maize yields significantly (P<0.05). However, higher yields were necessary but not sufficient criteria to determine profitability of different treatments. Manure + 60 kg N/ha gave highest NPV (USD 564), Manure + 40kg N/ha gave second highest NPV (USD 511) in Maragwa District while Manure + 60kg N/ha gave highest NPV (USD 633) and Manure + 40kg N/ha second highest NPV (USD 618) in Kirinyaga District. These results suggested that higher N-levels were not necessarily the most economical. Use of organic resources with modest amounts of mineral fertilizers seemed more profitable and held the key to enhancement of nutrient budgets, food security and rural livelihoods.Natural resource capital, Net present values (NPV), Nutrient budgets, Smallholder farmers, Soil organic matter (SOM), Structural adjustment programs (SAPs), Crop Production/Industries, Land Economics/Use,
Using farmer-prioritized vertisol management options for enhanced green gram and tomato production in central Kenya
Green gram (Phaseolus aures L.) and tomato (Solanum lycopersicum L) are widely grown in the vertisols of the Mwea Irrigation Scheme in Kenya alongside the rice fields. Green gram can fix nitrogen and is grown for its highly nutritious and curative seeds while tomato is grown for its fruit rich in fiber, minerals and vitamins. The two can be prepared individually or together in a variety of ways including raw salads and/or cooked/fried. They together form significant delicacies consumed with rice, which is the major cash crop grown in the black cotton soils. The crops can grow well in warm conditions but tomato is fairly adaptable except under excessive humidity and temperatures that reduce yields. Socio-economic prioritization by the farming community and on-farm demonstrations of soil management options were instituted to demonstrate enhanced green gram and tomato production in vertisol soils of lower parts of Kirinyaga County (Mwea East and Mwea West districts). Drainage management was recognized by the farming community as the best option although a reduced number of farmers used drainage and furrows/ridges, manure, fertilizer and shifting options in that descending order. Non-availability of labour and/or financial cost for instituting these management options were indicated as major hindrances to adoption of the yield enhancing options. Labour force was contributed to mainly by the family alongside hiring (64.2%) although 28% and 5.2%, respectively used hired or family labour alone. The female role in farming activities dominated while the male role was minimal especially at weeding. The youth role was insignificant and altogether absent at marketing. Despite the need for labour at earlier activities (especially when soil management options needed to be instituted) it was at the marketing stage that this force was directed. Soils were considered infertile by 60% but 40% indicated that their farms had adequate fertility. Analysis showed that with ridging, farm yard manure and fertilizer improved soil fertility, crop growth and income considerably. Phosphate and zinc enhancement reduced alkalinity and sodicity. Green gram and tomato yields increased under ridges and farm yard manure application by between 17-25% which significantly enhanced household incomes.Key words: Green gram, Tomato, Vertisol management, Household income, Mwea, Keny
Enhancement of Grassland Production Through Integration of Forage Legumes in Semi-Arid Rangelands of Kenya
Livestock production in semi-arid rangelands of Kenya is limited by the seasonal quantity and quality of fodder. Kirkman & Carvalho (2003) stated that these inter- and intra-seasonal quality and quantity fluctuations result in nutrient deficits that severely limit livestock production potential. The objective of this experiment was to study the effect of three forage legumes on the production of natural pastures in semi-arid rangelands of Kenya
The prevalence of antibodies to Brucella abortus in marketed milk in Kenya and its public health implications
The risk of infection by milk-borne brucellosis is one reason for public health regulations which discourage informal milk markets that sell unpasteurized milk. However, these regulations are not generally implemented in many developing countries. Kenya is a typical example, with over 85% of milk sales passing through informal channels. Consumer practices to reduce or eliminate potential infection by milk-borne health hazards under these circumstances have rarely been studied.
Seasonal survey data were collected between January 1999 and January 2000 from informal milk market agents of various cadres and from households consuming unpasteurized milk in rural and urban locations in central Kenya. Respondents were randomly selected within production system (extensive and intensive) and human population density (urban, peri-urban and rural) strata. In addition, pasteurized and packaged milk samples from five processors were collected. Samples were screened for antibodies to Brucella abortus using the milk ring test (MRT) (unpasteurized milk) and indirect antibody ELISA (both unpasteurized and pasteurized milk).
Milk samples originating from farms in the extensive production system and those containing milk from many sources were associated with higher antibody detection proportions. Five percent of all raw milk samples collected from consumer households and 4% of samples collected from various levels of bulking of market samples were positive to the ELISA. There was poor to no agreement between the two antibody detection tests. All urban consumers and 96% of rural consumers of unpasteurized milk indicated that they boil the milk (in tea or otherwise) before consumption. The implications of these results on milk marketing in Kenya are discussed
Nested association mapping of stem rust resistance in wheat using genotyping by sequencing
We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum). Ten stem rust resistant wheat varieties were crossed to the susceptible line LMPG-6 to generate F6 recombinant inbred lines. The recombinant inbred line populations were phenotyped in Kenya, South Africa, and St. Paul, Minnesota, USA. By joint mapping of the 10 populations, we identified 59 minor and medium-effect QTL (explained phenotypic variance range of 1%- 20%) on 20 chromosomes that contributed towards adult plant resistance to North American Pgt races as well as the highly virulent Ug99 race group. Fifteen of the 59 QTL were detected in multiple environments. No epistatic relationship was detected among the QTL. While these numerous small- to medium-effect QTL are shared among the families, the founder parents were found to have different allelic effects for the QTL. Fourteen QTL identified by joint mapping were also detected in single-population mapping. As these QTL were mapped using SNP markers with known locations on the physical chromosomes, the genomic regions identified with QTL could be explored more in depth to discover candidate genes for stem rust resistance. The use of GBS-derived de novo SNPs in mapping resistance to stem rust shown in this study could be used as a model to conduct similar markertrait association studies in other plant species.This is an open access article, free of all copiright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication
Genetic determinants of glucose-6-phosphate dehydrogenase activity in Kenya
Background:
The relationship between glucose-6-phosphate dehydrogenase (G6PD) deficiency and clinical phenomena such as primaquine-sensitivity and protection from severe malaria remains poorly defined, with past association studies yielding inconsistent and conflicting results. One possibility is that examination of a single genetic variant might underestimate the presence of true effects in the presence of unrecognized functional allelic diversity.
Methods:
We systematically examined this possibility in Kenya, conducting a fine-mapping association study of erythrocyte G6PD activity in 1828 Kenyan children across 30 polymorphisms at or around the G6PD locus.
Results:
We demonstrate a strong functional role for c.202G>A (rs1050828), which accounts for the majority of variance in enzyme activity observed (P=1.5 × 10-200, additive model). Additionally, we identify other common variants that exert smaller, intercorrelated effects independent of c.202G>A, and haplotype analyses suggest that each variant tags one of two haplotype motifs that are opposite in sequence identity and effect direction. We posit that these effects are of biological and possible clinical significance, specifically noting that c.376A>G (rs1050829) augments 202AG heterozygote risk for deficiency trait by two-fold (OR = 2.11 [1.12 - 3.84], P=0.014).
Conclusions:
Our results suggest that c.202G>A is responsible for the majority of the observed prevalence of G6PD deficiency trait in Kenya, but also identify a novel role for c.376A>G as a genetic modifier which marks a common haplotype that augments the risk conferred to 202AG heterozygotes, suggesting that variation at both loci merits consideration in genetic association studies probing G6PD deficiency-associated clinical phenotypes. </p
The impact of malaria-protective red blood cell polymorphisms on parasite biomass in children with severe Plasmodium falciparum malaria
Severe falciparum malaria is a major cause of preventable child mortality in sub-Saharan Africa. Plasma concentrations of P. falciparum Histidine-Rich Protein 2 (PfHRP2) have diagnostic and prognostic value in severe malaria. We investigate the potential use of plasma PfHRP2 and the sequestration index (the ratio of PfHRP2 to parasite density) as quantitative traits for case-only genetic association studies of severe malaria. Data from 2198 Kenyan children diagnosed with severe malaria, genotyped for 14 major candidate genes, show that polymorphisms in four major red cell genes that lead to hemoglobin S, O blood group, α-thalassemia, and the Dantu blood group, are associated with substantially lower admission plasma PfHRP2 concentrations, consistent with protective effects against extensive parasitized erythrocyte sequestration. In contrast the known protective ATP2B4 polymorphism is associated with higher plasma PfHRP2 concentrations, lower parasite densities and a higher sequestration index. We provide testable hypotheses for the mechanism of protection of ATP2B4
Comparison of the prevalence and characteristics of inpatient adverse events using medical records review and incident reporting
Background. Information on adverse events (AEs) in hospitalised patients in developing countries is scanty.Objective. To compare the magnitude and characteristics of inpatient AEs in a tertiary, not-for-profit healthcare facility in Kenya, using medical records review and incident reporting.Methods. Estimation of prevalence was done using incidents reported in 2010 from a random sample of medical records for hospital admissions. Nurse reviewers used 18 screening criteria, followed by physician reviewers to confirm occurrence. An AE was defined as an unexpected clinical event (UE) associated with death, disability or prolonged hospitalisation not explained by the disease condition. The kappa statistic was used to estimate inter-rater agreement, and analysis was done using logistic regression.Results. The study identified 53 UEs from 2 000 randomly selected medical records and 33 reported UEs from 23 026 admissions in the index year. The prevalences of AEs from medical records review and incident reports were 1.4% (95% confidence interval (CI) 0.9 - 2.0) and 0.03% (95% CI 0.012 - 0.063), respectively. Compared with incident reporting, review of medical records identified more disability (13.2% v. 0%; p=0.03) and prolonged hospital stays (43.4% v. 18.2%; p=0.02).Conclusions. Review of medical records is preferable to incident reporting in determining the prevalence of AEs in health facilities with limited inpatient quality improvement experience. Further research is needed to determine whether staff education and a positive culture change through promotion of non-punitive UE reporting or a combination of approaches would improve the comprehensiveness of AE reporting
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