Production of peach palm (Bactris gasipaes) for palm heart in different polyculture systems.

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Production of cupuaçu (Theobroma grandiflorum) in different polyculture systems.

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Production of annato (Bixa orellana L.) in an agroforestry system.

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Evaluation By Blue Native Polyacrylamide Electrophoresis Colorimetric Staining Of The Effects Of Physical Exercise On The Activities Of Mitochondrial Complexes In Rat Muscle.

Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial complexes I and IV in rat muscles with different morphological and biochemical properties. We also investigated these activities at different times after acute exercise of rat soleus muscle. Although having fewer mitochondria than oxidative muscles, white gastrocnemius muscle presented a significantly higher activity (26.7 +/- 9.5) in terms of complex I/V ratio compared to the red gastrocnemius (3.8 +/- 0.65, P < 0.05) and soleus (9.8 +/- 0.9, P < 0.001) muscles. Furthermore, the complex IV/V ratio of white gastrocnemius muscle was always significantly higher when compared to the other muscles. Ninety-five minutes of exhaustive physical exercise induced a decrease in complex I/V and complex IV/V ratios after all resting times (0, 3 and 6 h) compared to control (P < 0.05), probably reflecting the oxidative damage due to increasing free radical production in mitochondria. These results demonstrate the possible and useful application of BN-PAGE-histochemical staining to physical exercise studies.37939-4

Development and production of coconut palm (Cocos nucifera L.) in an agroforestry system.

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Avaliação de genótipos de cebola submetidos a diferentes regimes hídricos.

O objetivo do trabalho foi avaliar o desempenho de genótipos de cebola submetidos a diferentes níveis de irrigação. O experimento foi conduzido em casa de vegetação na Embrapa Hortaliças, Brasília-DF. Os tratamentos consistiram da combinação de doze genótipos (?Optima F1?, Grano TX-08, CNPH 6179org, HE-1, HE-2, HE-3, HE-4, HE-5, HE-6, HE-7, HE-8 e HE-9) com três tensões de água no solo (20, 45 e 70 kPa). A evapotranspiração total variou de 13,7 a 49,8 L (6 plantas/vaso 10 L), sendo ?Optima F1? e HE-3 os genótipos com menor ETc. Grano TX-08 foi mais produtivo, enquanto HE-3, HE-6 e ?Optima F1? foram os com menor produtividade. Houve redução da produtividade com o aumento do déficit hídrico para todos os genótipos e interação significativa entre ambos os fatores. Grano TX-08 e HE-6 foram respectivamente os genótipos com maior e menor eficiência do uso de água pelas plantas. O fator de sensibilidade hídrica (Ky) variou entre 0,74 (Grano TX-08) e 1,37 (?Optima F1?). Além do Grano TX-08, o genótipo CNPH 6179org demonstrou potencial para ser utilizado em programas de melhoramento visando tolerância a seca, dada à sua moderada sensibilidade ao déficit hídrico, alta eficiência no uso de água e alta produtividadeResumo 54-1

First insights on the differential expression of adaptive candidate genes among contrasting pathotypes of Hemileia vastatrix

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