Batista, D.

Diniz, I.

Guerra-Guimarães, L.

Loureiro, A.

Macedo, C.

Silva, M.C.

Várzea, V.

English

UTL Repository

www.alphavisa.com/asic/2021Posters  - Session 2 - Posters Session 2: Plant pathology & protection23428th Conference of Association for the Science and Information on Coffee28 June - 1 July 2021 Montpellier, FranceBook of abstractsS2-P-06First insights on the differential expression of adaptive candidate genes among contrasting pathotypes of Hemileia vastatrixBatista Dora (dccastro@fc.ul.pt), Macedo Cíntia, Diniz Inês, Loureiro Andreia, Várzea Vitor,  Guerra-Guimarães Leonor, Silva Maria do CéuCIFC/LEAF, ISA, Universidade de Lisboa, Oeiras, PortugalRATIONALEHemileia vastatrix (Hv) causing coffee leaf rust, remains the major threat to Arabica coffee production worldwide. Under the constant risk of new Hv pathotypes emerging under a strong selective pressure, a better understanding of the adaptive genetic variation of Hv populations is needed. Since genes involved in coffee-rust interaction are expected to evolve under strong selection, this study aimed at the analysis of expression differences in putative candidate genes under positive selection that could provide insights on the pathogen virulence evolution.METHODSIn this study, we selected seven candidate genes (CGs) with a signature of positive selection identified in a previous genome-wide scan integrated into a phylogenomics framework (Silva et al., 2015). To assess their potential association with Hv virulence profiles, expression analysis of CGs was initiated by qPCR for five isolates with contrasting pathotypes during compatible interactions at three key stages of the infection process [penetration stage (24hpi), >50% infection sites with haustoria (96hpi) and a stage preceding sporulation (8-11dpi)].RESULTSqPCR data analysis showed that most of the CGs studied were mainly activated during the penetration phase, suggesting their involvement in the early stages of the infection. Comparison between Hv isolates revealed significant differential expression of CGs involved in signaling for Hv1427 (lowest virulent pathotype) at the two first key stages of infection. However, in general, largest differences of expression were detected at different infection stages for each isolate, rather than among isolates at each time point.CONCLUSIONS & PERSPECTIVESFirst results revealed differences in expression among isolates, either regarding up or down-regulation at different infection stages, or the level of expression at each time point studied, suggesting that these candidate genes may be involved in Hv virulence. Putative causal relations and possible adaptive significance is being assessed. This study provides a first insight on the molecular variation underlying virulence divergence in coffee rust.Acknowledgments: Funding from PORLisboa, Portugal 2020 and European Union (FEDER) [LISBOA-01-0145-FEDER-029189], and Foundation for Science and Technology (FCT) under project PTDC/ASP-PLA/29189/2017. AL acknowledge DL57/2016/CP1479/CT0002.References:• Silva et al. PLoS ONE, 2015, 10(12):e0143959, DOI:10.1371/journal.pone.0143959

First insights on the differential expression of adaptive candidate genes among contrasting pathotypes of Hemileia vastatrix

https://www.repository.utl.pt/bitstream/10400.5/23286/1/Batista%20et%20al.%20Asic2021.pdf

First insights on the differential expression of adaptive candidate genes among contrasting pathotypes of Hemileia vastatrix

Abstract

Similar works

Full text

Available Versions

UTL Repository