44 research outputs found

    Lipase And Esterase - To What Extent Can This Classification Be Applied Accurately? [lipases E Esterases: Como Definir E Classificar?]

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    Enzyme technology is an ever-growing field of knowledge and, in recent years, this technology has raised renewed interest, due to the search for new paradigms in several productive processes. Lipases, esterases and cutinases are enzymes used in a wide range of processes involving synthesis and hydrolysis reactions. The objective of this work was to investigate and compare the specific lipase and esterase activities of five enzymes - four already classified as lipases and one classified as cutinase - in the presence of natural and synthetic substrates. All tested enzymes presented both esterase and lipase specific activities. The highest specific esterase activity was observed for Aspergillus 1068 lipase in natural substrate and for F. oxysporum cutinase in synthetic substrate, while the highest specific lipase activity was observed for Geotrichum sp. lipase in natural substrate and for F. oxysporum cutinase in synthetic substrate. These results display some interface-independent lipolytic activity for all lipases tested. This is in accordance with the rationale that a new and broader definition of lipases may be necessary.313608613Becker, P., Determination of the kinetic parameters during continuous cultivation of the lipase producing thermophile Bacillus sp. on olive oil (1997) Applied Microbiology and Biotechnology, 48 (2), pp. 184-190. , http://dx.doi.org/10.1007/s002530051036Bencharit, S., Structural insights into CPT-11 activation by mammalian carboxylesterases (2002) Nature Structural Biology, 9 (5), pp. 337-342. , http://dx.doi.org/10.1038/nsb790, PMid:11967565Bencharit, S., Crystal structure of human carboxylesterase 1 complexed with the Alzheimer's drug tacrine: From binding promiscuity to selective inhibition (2003) Chemistry & Biology, 10 (4), pp. 341-349. , http://dx.doi.org/10.1016/S1074-5521(03)00071-1Bencharit, S., Structural basis of heroin and cocaine metabolism by a promiscuous human drug-processing enzyme (2003) Nature Structural Biology, 10 (5), pp. 345-356. , http://dx.doi.org/10.1038/nsb919, PMid:12679808Bornscheuer, U.T., Microbial carboxyl esterases: Classification, properties and application in biocatalysis (2002) FEMS Microbiology Reviews, 26 (1), pp. 73-81. , http://dx.doi.org/10.1111/j.1574-6976.2002.tb00599.x, PMid:12007643Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analytical Biochemistry, 72, pp. 248-254. , http://dx.doi.org/10.1016/0003-2697(76)90527-3(2007), http://www.brenda-enzymes.info, BRENDA. 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(Eds.), Braunschweig: GBF MonographsEttinger, W.F., Thukral, S.K., Kolattukudy, P.E., Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi (1987) Biochemistry, 26, pp. 7883-7892. , http://dx.doi.org/10.1021/bi00398a052Ghamgui, H., Karra-Chaâbouni, M., Gargouri, Y., 1-Butyl oleate synthesis by immobilized lipase from n-hexane and solvent-free system (2004) Enzyme and Microbial Technology, 35 (4), pp. 355-363. , http://dx.doi.org/10.1016/j.enzmictec.2004.06.002Helistö, P., Korpela, T., Effects of detergents on activity of microbial lipases as measured by the paranitrophenyl alkanoate esters method (1998) Enzyme and Microbial Technology, 23 (1-2), pp. 113-117. , http://dx.doi.org/10.1016/S0141-0229(98)00024-6Holmquist, M., Alpha Beta-Hydrolase Fold Enzymes Structures, Functions and Mechanisms (2000) Current Protein and Peptide Science, 1 (2), pp. 209-235. , http://dx.doi.org/10.2174/1389203003381405, PMid:12369917(2007) Esters Database, , www.ensam.inra.fr/, INLAND NORTHWEST RESEARCH ALLIANCE-INRA, Montpellier: INRA. Disponível emKim, G.S., Screening, production and properties of a stereospecific esterase from Pseudomonas sp. S34 with high selectivity to (S)-ketoprofen ethyl ester (2002) Journal of Molecular Catalysis B: Enzymatic, 17 (1), pp. 29-38. , http://dx.doi.org/10.1016/S1381-1177(01)00077-7Kolattukudy, P.E., Cutinases from fungi and pollen (1984) Lipases, pp. 471-504. , BORGSTROM, B.BROCKMAN, H. (Eds.), Amsterdam: ElsevierKulkarni, N., Gadre, R.V., Production and properties of alkaline, thermophilic lipase from Pseudomonas fluorenscens NS2W (2002) Journal of Industrial Microbiology and Biotechnology, 28 (6), pp. 344-348. , http://dx.doi.org/10.1038/sj.jim.7000254, PMid:12032808Labuschagne, R.B., van Tonder, A., Litthauer, D., Flavobacterium odoratum lipase: Isolation and characterization (1997) Enzyme Microbial Technology, 21 (1), pp. 52-58http://dx.doi.org/10.1016/S0141-0229(96)00226-8Lin, Y.C., Wu, J.Y., Chen, T.L., Production of Acinetobacter radioresistens lipase with repeated batch culture in presence of nonwoven fabric (2001) Biotechnology and Bioengineering, 76 (3), pp. 214-218. , http://dx.doi.org/10.1002/bit.1185, PMid:11668456Longhi, S., Cambillau, C., Structure-activity of cutinase, a small lipolytic enzyme (1999) Biochimica Et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1441 (2-3), pp. 185-196Macedo, G.A., Produção, purificação, caracterização bioquímica e aplicações de lipase de Geotrichum sp (1995) Dissertação, 121, p. 1995. , (Mestrado em Tecnologia dos Alimentos)-Universidade Estadual de Campinas, CampinasMacedo, G.A., Park, Y.K., Pastore, G.M., Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp (1997) Brazilian Journal of Microbiology, 39, pp. 687-692Macedo, G.A., Pio, T.F., A rapid screening method for cutinase producing microorganisms (2005) Brazilian Journal of Microbiology, 36 (4), pp. 388-394. , http://dx.doi.org/10.1590/S1517-83822005000400016Mahadik, N.D., (2002) Production of Acidic Lipase By Aspergillus Niger In Solid State Fermentation. 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    Comparison of text-messaging to voice telephone interviews for active surveillance of adverse events following immunisation

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    Objectives: In 2013, the Follow-up and Active Surveillance of Trivalent Influenza Vaccine in Mums (FASTMum) program began using short message service (SMS) to collect adverse event information in pregnant women who recently received trivalent influenza vaccine (TIV). This study was designed to compare data collected via SMS and telephone for the purposes of monitoring vaccine safety. Methods: A number of 344 women who received TIV were randomly assigned to a telephone interview group. They were telephoned seven days post-vaccination and administered a standard survey soliciting any adverse events following immunisation (AEFI) they experienced. They were matched by brand of vaccine, age group, and residence to 344 women who were sent a SMS seven days post-vaccination. The SMS solicited similar information. AEFI reported by SMS and telephone interview were compared by calculating risk ratios. Results: Response rate was higher to SMS compared to telephone interview (90.1% vs. 63.9%). Women who were surveyed by SMS were significantly less likely to report an AEFI compared to women who were surveyed by telephone (RR: 0.41; 95% CI: 0.29–0.59). The greatest discrepancies between SMS and telephone interview were for self-reported injection site reactions (3.1% vs. 16.8%) and unsolicited (or “other”) events (11.4% vs. 4.1%). Data collected by SMS was significantly timelier. Conclusions: Data collection by SMS results in significantly improved response rates and timeliness of vaccine safety data. Systems which incorporate SMS could be used to more rapidly detect safety signals and promote more rapid public health response to vaccine quality issues

    Trabalho, terra e geração de renda em três décadas de reflorestamentos no alto Jequitinhonha.

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    A partir dos anos 1970, as chapadas do Alto Jequitinhonha, localizadas no nordeste de Minas Gerais, até então áreas de uso comum de agricultores familiares, foram plantadas com eucaliptos. O objetivo deste artigo é comparar os efeitos do reflorestamento sobre a estrutura fundiária, valor da produção agrícola e ocupação rural com os efeitos da produção agrícola familiar sobre essas mesmas variáveis na microrregião homogênea de Capelinha, no Alto Jequitinhonha. Tal microrregião foi escolhida para o estudo por ser a área de maior concentração de eucaliptais da região. Foram utilizados dados secundários dos Censos do IBGE para os anos de 1970, 1980, 1985 e 1996, além de dados secundários de pesquisas realizadas sobre a região em questão, incluindo entrevistas com dirigentes de empresas e lideranças sindicais. O artigo conclui que, em trinta anos, o reflorestamento concentrou terras e criou um número reduzido de empregos; a agricultura familiar, ao contrário, teve suas áreas de terras comprimidas e super-exploradas em decorrência da perda das chapadas, mas continuou sendo a principal responsável pela geração de ocupações e rendas na região

    Status Report Of The Schenberg Gravitational Wave Antenna

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    Here we present a status report of the Schenberg antenna. In the past three years it has gone to a radical upgrading operation, in which we have been installing a 1K pot dilution refrigerator, cabling and amplifiers for nine transducer circuits, designing a new suspension and vibration isolation system for the microstrip antennas, and developing a full set of new transducers, microstrip antennas, and oscillators. We are also studying an innovative approach, which could transform Schenberg into a broadband gravitational wave detector.3631Aguiar, O.D., (2002) Class. Quantum Grav., 19, p. 1949Aguiar, O.D., (2004) Class. Quantum Grav., 21, pp. S457Aguiar, O.D., (2005) Class. Quantum Grav., 22, pp. S209Aguiar, O.D., (2006) Class. Quantum Grav., 23, pp. S239Aguiar, O.D., (2008) Class. Quantum Grav., 25, p. 114042Costa, C.A., (2008) Class. Quantum Grav., 25, p. 184002Johnson, W.W., Merkowitz, S.M., (1993) Phys. Rev. Lett., 70, p. 2367Coccia, E., Lobo, J.A., Ortega, J.A., (1995) Phys. Rev. D, 52, p. 3735Thorne, K.S., (1978) Phys. Rev. Lett., 40, p. 667Tobar, M.E., Ivanov, E.N., Blair, D.G., (2000) Gen. Rel. Grav., 32, p. 1799De Waard, (2005) Class. Quantum Grav., 22, pp. S215Vinet, J.-Y., (2010) Research in Astron Astrophys., 10, p. 956Costa, C.A., Aguiar, O.D., Magalhães, N.S., (2004) Class. Quantum Grav., 21, pp. S827Forward, R.L., (1971) Gen. Rel. Grav., 2, p. 149Eardley, D.M., Lee, D.L., Lightman, A.P., Wagoner, R.V., Will, C.M., (1973) Phys. Rev. Lett., 30, p. 884Bianchi, M., Coccia, E., Colacino, C.N., Fafone, V., Fucito, F., (1996) Class. Quantum Grav., 13, p. 2865Andrade, L.A., (2009) Microwave and Optical Tech. Lett., 51, p. 1120Furtado, S.R., (2012), in preparationIvanov, E.N., Hartnett, J.G., Tobar, M.E., (2000) IEEE Trans. Ultrason., Ferroelect., Freq. Contr., 47, p. 1526Pimentel, G.L., (2008) J. Phys. Conf. Series, 122, p. 012028Aguiar, (2009) Int. J. Modern Phys. D, 18, p. 2317Furtado, S.R., (2009), Ph.D. Thesis at INPE, not publishedBraginsky, V.B., Vorontsov, Y.I., Thorne, K.S., (1980) Science, 209, p. 547Thorne, K.S., The Quantum Limit for Gravitational-Wave Detectors and Methods of Circumventing It (1979) Sources of Gravitational Waves, p. 49. , ed. L L Smarr, Cambridge University Press, Cambridge, US
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