Synergic Effect of α

Cervical cancer is the second leading cause of death among Mexican women. The treatment with cis-diamminedichloroplatinum (II) (CDDP) has some serious side effects. Alpha-mangostin (α-M), has a protective effect against CDDP-induced nephrotoxicity, as well as antioxidant, antitumor, and anti-inflammatory properties. Hence, we explored the in vitro and in vivo effect of α-M on human cervical cancer cell proliferation when combined with CDDP. In vitro, The cytotoxic effect of α-M and/or CDDP was measured by the 3-(3,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium assay. Meanwhile, apoptosis, reactive oxygen species (ROS) production, and the cell cycle were determined with flow cytometry. For α-M+CDDP treatment, both a coincubation and preincubation scheme were employed. In vivo, xenotransplantation was performed in female athymic BALB/c (nu/nu) mice, and then tumor volume and body weight were measured weekly, whereas α-M interfered with the antiproliferative activity of CDDP in the coincubation scheme, with preincubation with α-M+CDDP showing significantly greater cytotoxicity than CDDP or α-M alone, significantly inhibiting average tumor volume and preventing nephrotoxicity. This effect was accompanied by increased apoptosis and ROS production by HeLa cervical cancer cells, as well as an arrest in the cell cycle. These results suggest that α-M may be useful as a neoadjuvant agent in cervical cancer therapy

Implementación de servicios de red para infraestructura it a través de zentyal server 6.2

Página oficial de descarga de la distribución Zentyal Server 6.2Este artículo recopila la descripción detallada de la aplicación y funcionalidad de cinco (5) servicios diferentes, a partir de la implementación de la distribución Zentyal Server versión 6.2, dentro de una red informática.This article compiles the detailed description of the application and functionality of five (5) different services, starting from the implementation of the Zentyal Server version 6.2 distribution, within a computer network

Estimation of the real population and its impact on the utilisation of healthcare services in Mediterranean resort regions: an ecological study

BACKGROUND: The demographic structure has a significant influence on the use of healthcare services, as does the size of the population denominators. Very few studies have been published on methods for estimating the real population such as tourist resorts. The lack of information about these problems means there is a corresponding lack of information about the behaviour of populational denominators (the floating population or tourist load) and the effect of this on the use of healthcare services. The objectives of the study were: a) To determine the Municipal Solid Waste (MSW) ratio, per person per day, among populations of known size; b) to estimate, by means of this ratio, the real population in an area where tourist numbers are very significant; and c) to determine the impact on the utilisation of hospital emergency healthcare services of the registered population, in comparison to the non-resident population, in two areas where tourist numbers are very significant. METHODS: An ecological study design was employed. We analysed the Healthcare Districts of the Costa del Sol and the island of Menorca. Both are Spanish territories in the Mediterranean region. RESULTS: In the two areas analysed, the correlation coefficient between the MSW ratio and admissions to hospital emergency departments exceeded 0.9, with p < 0.001. On the basis of MSW generation ratios, obtained for a control zone and also measured in neighbouring countries, we estimated the real population. For the summer months, when tourist activity is greatest and demand for emergency healthcare at hospitals is highest, this value was found to be double that of the registered population. CONCLUSION: The MSW indicator, which is both ecological and indirect, can be used to estimate the real population in areas where population levels vary significantly during the year. This parameter is of interest in planning and dimensioning the provision of healthcare services

La degradación ambiental de los paisajes en las cuencas tributarias de la ensenada de Sibarimar (Guanabo e Itabo, Cuba)

The basins of the Guanabo and Itabo rivers are located in the northeaster limit of the La Habana province in Cuba. Meanwhile the urban and touristic uses of the territory are very significant in the coastline, the agriculture and the livestock are predominant in the hinterland. Just a few of natural areas remain in this area. Due to the conflict of uses and changing ecological features, the analysis of the environmental degradation of the landscapes is vital in order to establish a sustainable development of the territory. To reach this aim, we have classified the territory in units attending to the main ecological features that form the different landscapes. After that, we have carried out an integrated diagnosis taking into consideration aspects such as the main uses of the landscape units, its potential and efficiency, environmental dangers, degrading processes and geoecological state.Las cuencas de los ríos Guanabo e Itabo se localizan en el límite nororiental de la provincia de La Habana en Cuba. Mientras que los usos urbanos y turísticos son muy importantes en la costa, la agricultura y la ganadería son predominantes en el interior. Solamente quedan unas pocas áreas naturales. Debido al conflicto de usos y a las cambiantes características ecológicas es vital el análisis de la degradación medioambiental de los paisajes para establecer un desarrollo sostenible del territorio. Para alcanzar este objetivo hemos clasificado el territorio en unidades según las principales características que conforman los diferentes paisajes. Después de eso, hemos realizado un diagnóstico integrado teniendo en consideración aspectos como los principales usos de las unidades del paisaje, su potencial y eficiencia, los peligros ambientales, los procesos degradantes y el estado geoecológico.Les bassins hydrographiques des fleuves Itabo et Guanabo sont situés sur la limite nord de la province de La Havane, à Cuba. Alors que l'usages urbains et touristiques sont très importants sur la côte, de l'agriculture et l'élevage sont prédominant dans l'intérieur. Ils ne restent plus quelques zones naturelles résiduelles. En raison des conflits d'usages et de l'évolution des caractéristiques écologiques c'est très important faire d'analyse essentielle de la dégradation de l'environnement du paysage, pour établir un développement durable des terres. Pour ce faire, il faut divisé le territoire en unités suivant les caractéristiques qui ont les différents paysages. Après cela, on fera un diagnostic intégré en train de considérer des aspects tels que les prin- cipaux/dominant utilisations des unités de paysage, le potentiel et l'efficience, les risques et la dégradation sur l'environnement, et aussi l'état géoécologique

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P &lt; 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)