Quasi-two-body decays B→ηc(1S,2S) [ρ(770),ρ(1450),ρ(1700)→] ππB \to \eta_c {(1S ,2S)}\;[\rho(770),\rho(1450),\rho(1700) \to ]\; \pi\pi in the perturbative QCD approach

In this paper, we calculated the branching ratios of the quasi-two-body decays $B \to \eta_c (1S ,2S)$ $[\rho(770), \rho(1450),\rho(1700)\to ] \pi\pi$ by employing the perturbative QCD (PQCD) approach. The contributions from the $P$-wave resonances $\rho(770)$, $\rho(1450)$ and $\rho(1700)$ were taken into account. The two-pion distribution amplitude $\Phi_{\pi\pi}^{\rm P}$ is parameterized by the vector current time-like form factor $F_{\pi}$ to study the considered decay modes. We found that (a) the PQCD predictions for the branching ratios of the considered quasi-two-body decays are in the order of $10^{-7} \sim 10^{-6}$, while the two-body decay rates ${\cal B}(B \to \eta_c{(1S,2S)} (\rho(1450),\rho(1700)))$ are extracted from those for the corresponding quasi-two-body decays; (b) the whole pattern of the pion form factor-squared $|F_\pi|^2$ measured by the BABAR Collaboration could be understood based on our theoretical results; (c) the general expectation based on the similarity between $B \to \eta_c \pi\pi$ and $B \to J/\psi \pi\pi$ decays are confirmed: $R_2(\eta_c)\approx 0.45$ is consistent with the measured $R_2(J/\psi)\approx 0.56\pm 0.09$ within errors; and (d) new ratios $R_3(\eta_c(1S))$ and $R_4(\eta_c(2S))$ among the branching ratios of the considered decay modes are defined and could be tested by future experiments.Comment: 10 pages, 3 figure

Quasi-two-body decays B→DK∗(892)→DKπB \to D K^*(892) \to D K \pi in the perturbative QCD approach

We study the quasi-two-body decays $B\to D K^*(892) \to D K\pi$ by employing the perturbative QCD approach. The two-meson distribution amplitudes \Phi_{K\pi}^{\text{P-wave}} are adopted to describe the final state interactions of the kaon-pion pair in the resonance region. The resonance line shape for the $P$-wave $K\pi$ component $K^*(892)$ in the time-like form factor $F_{K\pi}(s)$ is parameterized by the relativistic Breit-Wigner function. For most considered decay modes, the theoretical predictions for their branching ratios are consistent with currently available experimental measurements within errors. We also disscuss some ratios of the branching fractions of the concerned decay processes. More precise data from LHCb and Belle-II are expected to test our predictions.Comment: 10 pages, 3 figures and 2 tables.To be published in EPJ

Fat suppression for ultrashort echo time imaging using a novel soft-hard composite radiofrequency pulse.

PurposeTo design a soft-hard composite pulse for fat suppression and water excitation in ultrashort echo time (UTE) imaging with minimal short T2 signal attenuation.MethodsThe composite pulse contains a narrow bandwidth soft pulse centered on the fat peak with a small negative flip angle (-α) and a short rectangular pulse with a small positive flip angle (α). The fat magnetization experiences both tipping-down and -back with an identical flip angle and thus returns to the equilibrium state, leaving only the excited water magnetization. Bloch simulations, as well as knee, tibia, and ankle UTE imaging studies, were performed to investigate the effectiveness of fat suppression and corresponding water signal attenuation. A conventional fat saturation (FatSat) module was used for comparison. Signal suppression ratio (SSR), defined as the ratio of signal difference between non-fat-suppression and fat-suppression images over the non-fat-suppression signal, was introduced to evaluate the efficiency of the composite pulse.ResultsNumerical simulations demonstrate that the soft-hard pulse has little saturation effect on short T2 water signals. Knee, tibia, and ankle UTE imaging results suggest that comparable fat suppression can be achieved with the soft-hard pulse and the FatSat module. However, much less water saturation is induced by the soft-hard pulse, especially for short T2 tissues, with SSRs reduced from 71.8 ± 6.9% to 5.8 ± 4.4% for meniscus, from 68.7 ± 5.5% to 7.7 ± 7.6% for bone, and from 62.9 ± 12.0% to 4.8 ± 3.2% for the Achilles tendon.ConclusionThe soft-hard composite pulse can suppress fat signals in UTE imaging with little signal attenuation on short T2 tissues

Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates

To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in comparison with the reference strain PG21, which is susceptible to fluoroquinolones antibiotics. 15 MH isolates with three kinds of quinolone resistance phenotypes were obtained. Thirteen out of these quinolone-resistant isolates were found to carry nucleotide substitutions in either gyrA or parC. There were no alterations in gyrB and no mutations were found in the isolates with a phenotype of resistance to Ofloxacin (OFX), intermediate resistant to Levofloxacin (LVX) and Sparfloxacin (SFX), and those susceptible to all three tested antibiotics. The molecular mechanism of fluoroquinolone resistance in clinical isolates of MH was reported in this study. The single amino acid mutation in ParC of MH may relate to the resistance to OFX and LVX and the high-level resistance to fluoroquinolones for MH is likely associated with mutations in both DNA gyrase and the ParC subunit of topoisomerase IV

Molecular docking studies on rocaglamide, a traditional Chinese medicine for periodontitis

Purpose: To undertake an in silico assessment of rocaglamide as a potential drug therapy forperiodontitis (dental arthritis).Method: Lamarckian algorithm-based automated docking approach using AutoDock4.2 tool wasapplied for calculating the best possible binding mode of rocaglamide to IL-23p19 and IL-17, the targets of anti-inflammatory drugs in periodontal disease.Results: The top two interactions of rocaglamide with IL-17 (ΔG = -5.45 and -4.83 kcal/mol) were more spontaneous, and the physical interactions (two hydrogen bonds and one π-πbond) generated in the two IL-17- rocaglamide complexes were higher in number than in IL-23p14-rocaglamide complexes.Conclusion: In silico analysis of rocaglamide, a known antimicrobial and anti-inflammatory agent, is a promising natural candidate for periodontitis therapy, and should be further subjected to in vitro and in vivo anti-periodontitis investigations.Keywords: Periodontitis, Inflammation, Rocaglamide, Molecular docking, Lamarckian algorithm, IL- 23p19, IL-1

Trinitrophenol Reactive T-Cell Hybridomas Recognize Antigens That Require Antigen Processing

Protein antigens must be taken up, processed, and displayed on the surface of antigen-presenting cells in association with major histocompatibility complex molecules before they can be recognized by T cells. Whether recognition of the haptens used to study allergic contact hypersensitivity in murine models similarly requires processing has not been determined. We analyzed whether presentation of trinitrophenol to trinitrophenol reactive T-cell hybridomas requires antigen processing by studying the effects of inhibitors of antigen processing and presentation on tile ability of a syngeneic B-cell tumor (A20) to present trinitrophenol to a series of interleukin-2 producing, trinitrophenol specific, major histocompatibility complex class II-restricted T-cell hybridomas.The ability of trinitrophenol modified A20 cells to stimulate the hybridomas was completely inhibited by rnonoclonal, anti-trinitrophenol, or anti-Ia antibodies and was significantly reduced by paraformaldehyde fixation immediately after trinitrophenol modification. Trinitrophenol-modified A20 cultured at 37°C for 2h prior to fixation was significantly more effective at stimulating the hybridomas than trinitrophenol-modified A20 to present trinitrophenol was inhibited by chloroquine. Paraformaldehyde fixation and chloroquine treatment had similar effects on the ability of trinitrophenol modified lymph node dendritic cells to stimulate the trinitrophenol specific hybridomas. Paraformaldehyde fixation and chloroquine treatment had similar effects on the ability of A20 cells to present ovalbumin to ovalbumin-specific hybridomas as they had on the ability of trinitrophenol modified A20 cells to present trinitrophenol to the trinitrophenol specific hybridomas. One of seven T-cell hybridomas responded to trinitrophenol modified ovalbumin but not other trinitrophenol modified proteins. These results suggest that, at least in part, T cells in the contact hypersensitivity response to trinitrophenol recognize antigens that require processing and that trinitrophenol modified proteins can be recognized

AcidoCEST-UTE MRI for the Assessment of Extracellular pH of Joint Tissues at 3 T.

ObjectivesThe goal of this study was to demonstrate feasibility of measuring extracellular pH in cartilage and meniscus using acidoCEST technique with a 3-dimensional ultrashort echo time readout (acidoCEST-UTE) magnetic resonance imaging (MRI).Materials and methodsMagnetization transfer ratio asymmetry, radiofrequency (RF) power mismatch, and relative saturation transfer were evaluated in liquid phantoms for iopromide, iopamidol, and iohexol over a pH range of 6.2 to 7.8, at various agent concentrations, temperatures, and buffer concentrations. Tissue phantoms containing cartilage and meniscus were evaluated with the same considerations for iopamidol and iohexol. Phantoms were imaged with the acidoCEST-UTE MRI sequence at 3 T. Correlation coefficients and coefficients of variations were calculated. Paired Wilcoxon rank-sum tests were used to evaluate for statistically significant differences.ResultsThe RF power mismatch and relative saturation transfer analyses of liquid phantoms showed iopamidol and iohexol to be the most promising agents for this study. Both these agents appeared to be concentration independent and feasible for use with or without buffer and at physiologic temperature over a pH range of 6.2 to 7.8. Ultimately, RF power mismatch fitting of iohexol showed the strongest correlation coefficients between cartilage, meniscus, and fluid. In addition, ratiometric values for iohexol are similar among liquid as well as different tissue types.ConclusionsMeasuring extracellular pH in cartilage and meniscus using acidoCEST-UTE MRI is feasible

Knee menisci segmentation and relaxometry of 3D ultrashort echo time cones MR imaging using attention U-Net with transfer learning.

PurposeTo develop a deep learning-based method for knee menisci segmentation in 3D ultrashort echo time (UTE) cones MR imaging, and to automatically determine MR relaxation times, namely the T1, T1ρ , and T2∗ parameters, which can be used to assess knee osteoarthritis (OA).MethodsWhole knee joint imaging was performed using 3D UTE cones sequences to collect data from 61 human subjects. Regions of interest (ROIs) were outlined by 2 experienced radiologists based on subtracted T1ρ -weighted MR images. Transfer learning was applied to develop 2D attention U-Net convolutional neural networks for the menisci segmentation based on each radiologist's ROIs separately. Dice scores were calculated to assess segmentation performance. Next, the T1, T1ρ , T2∗ relaxations, and ROI areas were determined for the manual and automatic segmentations, then compared.ResultsThe models developed using ROIs provided by 2 radiologists achieved high Dice scores of 0.860 and 0.833, while the radiologists' manual segmentations achieved a Dice score of 0.820. Linear correlation coefficients for the T1, T1ρ , and T2∗ relaxations calculated using the automatic and manual segmentations ranged between 0.90 and 0.97, and there were no associated differences between the estimated average meniscal relaxation parameters. The deep learning models achieved segmentation performance equivalent to the inter-observer variability of 2 radiologists.ConclusionThe proposed deep learning-based approach can be used to efficiently generate automatic segmentations and determine meniscal relaxations times. The method has the potential to help radiologists with the assessment of meniscal diseases, such as OA

Spectroscopic Study of Globular Clusters in the Halo of M31 with Xinglong 2.16m Telescope II: Dynamics, Metallicity and Age

(Abridged) We performed the spectroscopic observations of 11 confirmed GCs in M31 with the Xinglong 2.16m telescope and we mainly focus on the fits method and the metallicity gradient for the M31 GC sample. We analyzed and discussed more about the dynamics, metallicity and age, and their distributions as well as the relationships between these parameters. Eight more confirmed GCs in the halo of M31 were observed, most of which lack the spectroscopic information before. These star clusters are located far from the galactic center at a projected radius of ~14 to ~117 kpc. The Lick absorption-line indices and the radial velocities have been measured and ages, metallicities [Fe/H] and alpha-element [alpha/Fe] have also been fitted by comparing the observed spectral feature indices and Thomas et al.SSP model. Our results show that most of the star clusters of our sample are older than 10 Gyr except B290 ~5.5 Gyr, and most of them are metal-poor with the metallicity [Fe/H]<-1, suggesting that these clusters were born at the early stage of the galaxy's formation. We find that the metallicity gradient for the outer halo clusters with r_p>25 kpc may not exist with a slope of -0.005+-0.005 dex kpc^-1. We also find that the metallicity is not a function of age for the GCs with age < 7 Gyr while for the old GCs with age >7 Gyr there seems to be a trend that the older ones have lower metallicity. Besides, We plot metallicity distributions with the largest sample of M31 GCs so far and it shows the bimodality is not significant and the number of the metal-poor and metal-rich groups becomes comparable. The spatial distributions shows that the metal-rich group is more centrally concentrated while the metal-poor group is occupy a more extended halo and the young population is centrally concentrated while the old population is more extended spatially to the outer halo.Comment: 17 pages, 10 figures and 6 tables. Accepted for publication in RA