Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds.

Previously, we only apply a traditional Chinese medicine (TCM) Danshen-Chuanxiong-Honghua (DCH) for cardioprotection via anti-inflammation in rats of acute myocardial infarction by occluding coronary artery. Presently, we select not only DCH but also its main absorbed compound ferulic acid (FA) for cerebra protection via similar action of mechanism above in animals of the transient middle cerebral artery occlusion (tMCAO). We investigated whether oral administration of DCH and FA could ameliorate MCAO-induced brain lesions in animals. By using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we analyzed four compounds, including tanshinol, salvianolic acid B, hydroxysafflor yellow A and especially FA as the putative active components of DCH extract in the plasma, cerebrospinal fluid and injured hippocampus of rats with MCAO. In our study, it was assumed that FA played a similar neuroprotective role to DCH. We found that oral pretreatment with DCH (10 or 20 g/kg) and FA (100 mg/kg) improved neurological function and alleviated the infarct volume as well as brain edema in a dose-dependent manner. These changes were accompanied by improved ischemia-induced apoptosis and decreased the inflammatory response. Additionally, chronic treatment with DCH reversed MCAO-induced spatial cognitive deficits in a manner associated with enhanced neurogenesis and increased the expression of brain-derived neurotrophic factor in lesions of the hippocampus. These findings suggest that DCH has the ability to recover cognitive impairment and offer neuroprotection against cerebral ischemic injury via inhibiting microenvironmental inflammation and triggering of neurogenesis in the hippocampus. FA could be one of the potential active compounds

The Role of XBP1 in bone metabolism

Bone is a dynamic organ that, once formed, undergoes a constant remodeling process that includes bone resorption and synthesis. Osteoclasts and osteoblasts are primarily responsible for controlling this process. X-box binding protein 1 (XBP1), a transcription factor, affects the metabolism of bones in various ways. In recent years, numerous studies have revealed that XBP1 plays a vital role in bone metabolism, including osteoclast and osteoblast development, as well as in regulating immune cell differentiation that affects the immune microenvironment of bone remodeling. In this review, we highlight the regulatory mechanisms of XBP1 on osteoclasts and osteoblasts, how XBP1 affects the immune microenvironment of bone remodeling by influencing the differentiation of immune cells, and predict the possible future research directions of XBP1 to provide new insights for the treatment of bone-related metabolic diseases

From Cleanroom to Desktop: Emerging Micro-Nanofabrication Technology for Biomedical Applications

This review is motivated by the growing demand for low-cost, easy-to-use, compact-size yet powerful micro-nanofabrication technology to address emerging challenges of fundamental biology and translational medicine in regular laboratory settings. Recent advancements in the field benefit considerably from rapidly expanding material selections, ranging from inorganics to organics and from nanoparticles to self-assembled molecules. Meanwhile a great number of novel methodologies, employing off-the-shelf consumer electronics, intriguing interfacial phenomena, bottom-up self-assembly principles, etc., have been implemented to transit micro-nanofabrication from a cleanroom environment to a desktop setup. Furthermore, the latest application of micro-nanofabrication to emerging biomedical research will be presented in detail, which includes point-of-care diagnostics, on-chip cell culture as well as bio-manipulation. While significant progresses have been made in the rapidly growing field, both apparent and unrevealed roadblocks will need to be addressed in the future. We conclude this review by offering our perspectives on the current technical challenges and future research opportunities

Charge Transport Properties of BO-Chelated Azadipyrromethenes

Intramolecular BO-chelated azadipyrromethenes are promising organic semiconductors. Here, we evaluated the electron and hole mobility of a series of BO-chelated azadipyrromethenes using the Space Charge Limited Current(SCLC) method. In order to determine the mobility of the material, Mott Gurney\u27s law was applied using the film thickness and slope of the J1/2 versus voltage plot in the SCLC graph. The best electron mobility observed for BO-chelated materials is 4.64—10-6 cm2V-1s-1 and the best hole mobility is 7.38—10-4 cm2V-1s-1. These results suggest that BO-chelated materials are promising p-type semiconductors for electronic applications.https://commons.case.edu/intersections-fa20/1028/thumbnail.jp

Deformation Measurements of Helicopter Rotor Blades Using a Photogrammetric System

As an important part of the helicopter, the rotor directly affects flight safety and flight quality. Knowledge of the rotor dynamic behaviors is significant for validating and optimizing the performance of the helicopter rotor system. In this study, a photogrammetric system, based on 3D point tracking and stereo photogrammetry technology, is presented to solve the full-field dynamic motion and deformation parameters of rotating blades by identifying the retro-reflective targets arranged on the rotor. The photogrammetric system is demonstrated in the wind tunnel tests of a 2 m-diameter model rotor, conducted at the 5.5 m × 4 m Aeroacoustic Wind Tunnel of the China Aerodynamics Research and Development Center (CARDC). With the targets attached on the special hat installed directly over the rotor hub, a unified rotor coordinate system, that was stationary with respect to the rotor, could be established at any measuring instant. Therefore, by transforming the 3D coordinates of all measured targets to the rotor coordinate system, the blade displacements and deformations at different test conditions could be calculated consistently. Experimental results from current study were compared to simulation results calculated by the comprehensive analytical model of rotorcraft aerodynamics and dynamics (CAMRAD), which shows quite good agreements

Deformation Measurements of Helicopter Rotor Blades Using a Photogrammetric System

As an important part of the helicopter, the rotor directly affects flight safety and flight quality. Knowledge of the rotor dynamic behaviors is significant for validating and optimizing the performance of the helicopter rotor system. In this study, a photogrammetric system, based on 3D point tracking and stereo photogrammetry technology, is presented to solve the full-field dynamic motion and deformation parameters of rotating blades by identifying the retro-reflective targets arranged on the rotor. The photogrammetric system is demonstrated in the wind tunnel tests of a 2 m-diameter model rotor, conducted at the 5.5 m × 4 m Aeroacoustic Wind Tunnel of the China Aerodynamics Research and Development Center (CARDC). With the targets attached on the special hat installed directly over the rotor hub, a unified rotor coordinate system, that was stationary with respect to the rotor, could be established at any measuring instant. Therefore, by transforming the 3D coordinates of all measured targets to the rotor coordinate system, the blade displacements and deformations at different test conditions could be calculated consistently. Experimental results from current study were compared to simulation results calculated by the comprehensive analytical model of rotorcraft aerodynamics and dynamics (CAMRAD), which shows quite good agreements

Full-Field Displacement Measurements of Helicopter Rotor Blades Using Stereophotogrammetry

This study presents a stereophotogrammetry approach to achieve full-field displacement measurements of helicopter rotor blades. The method is demonstrated in the wind tunnel test of a 2 m diameter rotor, conducted at the 5.5 m×4 m Aeroacoustic Wind Tunnel of China Aerodynamics Research and Development Center (CARDC). By arranging the retroreflective targets on the special hat installed directly above the rotor hub, the dynamic motion of the rotor shaft was tracked accurately, and a unified coordinate system was established on the rotor. Therefore, three-dimensional coordinates of instantaneously measured targets attached on the blade could be transformed to the unified rotor coordinate system, thereby providing a basis for consistently calculating the blade displacements at different test conditions. Moreover, location deviations of the blade caused by the vibration of the measuring system or the rotor due to freestream and rotor rotation were also effectively corrected through coordinate transformation. Comparisons of experimental and simulation results for a range of hover and forward flight conditions show good magnitude and trend agreements

Genome-Wide Identification, Evolution and Expressional Analysis of <i>OSCA</i> Gene Family in Barley (<i>Hordeum vulgare</i> L.)

The hyperosmolality-gated calcium-permeable channel gene family (OSCA) is one kind of conserved osmosensors, playing a crucial role in maintaining ion and water homeostasis and protecting cellular stability from the damage of hypertonic stress. Although it has been systematically characterized in diverse plants, it is necessary to explore the role of the OSCA family in barley, especially its importance in regulating abiotic stress response. In this study, a total of 13 OSCA genes (HvOSCAs) were identified in barley through an in silico genome search method, which were clustered into 4 clades based on phylogenetic relationships with members in the same clade showing similar protein structures and conserved motif compositions. These HvOSCAs had many cis-regulatory elements related to various abiotic stress, such as MBS and ARE, indicating their potential roles in abiotic stress regulation. Furthermore, their expression patterns were systematically detected under diverse stresses using RNA-seq data and qRT-PCR methods. All of these 13 HvOSCAs were significantly induced by drought, cold, salt and ABA treatment, demonstrating their functions in osmotic regulation. Finally, the genetic variations of the HvOSCAs were investigated using the re-sequencing data, and their nucleotide diversity in wild barley and landrace populations were 0.4966 × 10−3 and 0.391 × 10−3, respectively, indicating that a genetic bottleneck has occurred in the OSCA family during the barley evolution process. This study evaluated the genomic organization, evolutionary relationship and genetic expression of the OSCA family in barley, which not only provides potential candidates for further functional genomic study, but also contributes to genetically improving stress tolerance in barley and other crops