6,326 research outputs found
Flowering of kiwifruit (Actinidia deliciosa) is reduced by long photoperiods
Mature kiwifruit (Actinidia deliciosa âHaywardâ) vines grown under standard orchard management were exposed to 16-h photoperiods from the longest day in summer until after leaf fall in autumn. Photoperiod extension was achieved with tungsten halogen lamps that produced 2â8 ”mols mâ2 sâ1 photosynthetically active radiation. Long day treatments did not affect fruit dry matter or fruit weight at harvest during the growing season that the treatments were applied or during the following growing season. However, flowering was reduced by 22% during the spring following treatment application. As this reduction in flowering was not accompanied by a decrease in budbreak, the long day effect is not consistent with a delay in the onset of winter chilling. It is suggested therefore, that the observed reduction in flowering may be because of a diminution of floral evocation
Quantum states made to measure
Recent progress in manipulating quantum states of light and matter brings
quantum-enhanced measurements closer to prospective applications. The current
challenge is to make quantum metrologic strategies robust against
imperfections.Comment: 4 pages, 3 figures, Commentary for Nature Photonic
Broadband gradient impedance matching using an acoustic metamaterial for ultrasonic transducers
2016-2017 > Academic research: refereed > Publication in refereed journal201804_a bcmaVersion of RecordPublishe
Long-term clinical, immunologic and virologic impact of glucocorticoids on the chronic phase of HIV infection
BACKGROUND: To test the hypothesis of down-regulating the increased immune system activation/destruction process associated with chronic HIV infection, we focused our interest on prednisolone (PDN), because we had showed that, in vitro, PDN had a strong anti-apoptotic activity on activated T cells of HIV-infected patients and no effect on viral replication. We thus designed in 1992 a pilot study to evaluate the clinical, immunologic and virologic effects of PDN. The drug was given to a group of 44 patients with CD4 T cells over 200/Όl. After one year, no patient had developed clinical AIDS and the mean CD4 T cell count of the group had increased from 441 ± 21 cells/Όl to 553 ± 43 cells/Όl. Moreover, markers of immune activation had dropped back to normal levels while the mean viral load of the group had remained unchanged. Here we explore the long-term clinical, immunologic, and virologic impact of prednisolone on the chronic phase of HIV infection. METHODS: Retrospective study over 10 years starting between July 1992 and February 1993. A total of 44 patients with CD4 cells/Όl ranging from 207 to 775 were treated with prednisolone, 0.5 mg/kg/d, over 6 months and 0.3 mg/kg/d thereafter. RESULTS: No clinical AIDS developed under prednisolone; side effects of the drug were mild. CD4 cells which increased from 421 cells/Όl at entry to 625 cells/Όl at day 15, slowly decreased to reach 426 cells/Όl after two years; T cell apoptosis and activation markers dropped within 15 days to normal levels and reincreased slowly thereafter. Serum viral loads remained stable. The percentage of patients maintaining CD4 cells over entry was 43.2% at two years, 11.4% at five years and 4.6% at 10 years. Initial viral load was highly predictive of the rate of CD4 decrease under prednisolone. CONCLUSIONS: Prednisolone postponed CD4 cell decrease in a viral load dependent manner for a median of two years and for up to 10 years in a fraction of the patients with a low viral load. These findings might stimulate clinical trials as well as biological research on the role of antiapoptotic drugs in HIV infection
Current measurement by real-time counting of single electrons
The fact that electrical current is carried by individual charges has been
known for over 100 years, yet this discreteness has not been directly observed
so far. Almost all current measurements involve measuring the voltage drop
across a resistor, using Ohm's law, in which the discrete nature of charge does
not come into play. However, by sending a direct current through a
microelectronic circuit with a chain of islands connected by small tunnel
junctions, the individual electrons can be observed one by one. The quantum
mechanical tunnelling of single charges in this one-dimensional array is time
correlated, and consequently the detected signal has the average frequency
f=I/e, where I is the current and e is the electron charge. Here we report a
direct observation of these time-correlated single-electron tunnelling
oscillations, and show electron counting in the range 5 fA-1 pA. This
represents a fundamentally new way to measure extremely small currents, without
offset or drift. Moreover, our current measurement, which is based on electron
counting, is self-calibrated, as the measured frequency is related to the
current only by a natural constant.Comment: 9 pages, 4 figures; v2: minor revisions, 2 refs added, words added to
title, typos correcte
Monotone and fast computation of Eulerâs constant
Abstract We construct sequences of finite sums ( l Ë n ) n â„ 0 and ( u Ë n ) n â„ 0 converging increasingly and decreasingly, respectively, to the Euler-Mascheroni constant Îł at the geometric rate 1/2. Such sequences are easy to compute and satisfy complete monotonicity-type properties. As a consequence, we obtain an infinite product representation for 2 Îł converging in a monotone and fast way at the same time. We use a probabilistic approach based on a differentiation formula for the gamma process
Unusual Location of the Geotail Magnetopause Near Lunar Orbit: A Case Study
The Earth's magnetopause is highly variable in location and shape and is modulated by solar wind conditions. On 8 March 2012, the ARTEMIS probes were located near the tail current sheet when an interplanetary shock arrived under northward interplanetary magnetic field conditions and recorded an abrupt tail compression at âŒ(â60, 0, â5) RE in Geocentric Solar Ecliptic coordinate in the deep magnetotail. Approximately 10 minutes later, the probes crossed the magnetopause many times within an hour after the oblique interplanetary shock passed by. The solar wind velocity vector downstream from the shock was not directed along the SunâEarth line but had a significant Y component. We propose that the compressed tail was pushed aside by the appreciable solar wind flow in the Y direction. Using a virtual spacecraft in a global magnetohydrodynamic (MHD) simulation, we reproduce the sequence of magnetopause crossings in the XâY plane observed by ARTEMIS under oblique shock conditions, demonstrating that the compressed magnetopause is sharply deflected at lunar distances in response to the shock and solar wind VY effects. The results from two different global MHD simulations show that the shocked magnetotail at lunar distances is mainly controlled by the solar wind direction with a timescale of about a quarter hour, which appears to be consistent with the windsock effect. The results also provide some references for investigating interactions between the solar wind/magnetosheath and lunar nearside surface during full moon time intervals, which should not happen in general
Regulatory control and the costs and benefits of biochemical noise
Experiments in recent years have vividly demonstrated that gene expression
can be highly stochastic. How protein concentration fluctuations affect the
growth rate of a population of cells, is, however, a wide open question. We
present a mathematical model that makes it possible to quantify the effect of
protein concentration fluctuations on the growth rate of a population of
genetically identical cells. The model predicts that the population's growth
rate depends on how the growth rate of a single cell varies with protein
concentration, the variance of the protein concentration fluctuations, and the
correlation time of these fluctuations. The model also predicts that when the
average concentration of a protein is close to the value that maximizes the
growth rate, fluctuations in its concentration always reduce the growth rate.
However, when the average protein concentration deviates sufficiently from the
optimal level, fluctuations can enhance the growth rate of the population, even
when the growth rate of a cell depends linearly on the protein concentration.
The model also shows that the ensemble or population average of a quantity,
such as the average protein expression level or its variance, is in general not
equal to its time average as obtained from tracing a single cell and its
descendants. We apply our model to perform a cost-benefit analysis of gene
regulatory control. Our analysis predicts that the optimal expression level of
a gene regulatory protein is determined by the trade-off between the cost of
synthesizing the regulatory protein and the benefit of minimizing the
fluctuations in the expression of its target gene. We discuss possible
experiments that could test our predictions.Comment: Revised manuscript;35 pages, 4 figures, REVTeX4; to appear in PLoS
Computational Biolog
Generalized nonreciprocity in an optomechanical circuit via synthetic magnetism and reservoir engineering
Synthetic magnetism has been used to control charge neutral excitations for
applications ranging from classical beam steering to quantum simulation. In
optomechanics, radiation-pressure-induced parametric coupling between optical
(photon) and mechanical (phonon) excitations may be used to break time-reversal
symmetry, providing the prerequisite for synthetic magnetism. Here we design
and fabricate a silicon optomechanical circuit with both optical and mechanical
connectivity between two optomechanical cavities. Driving the two cavities with
phase-correlated laser light results in a synthetic magnetic flux, which in
combination with dissipative coupling to the mechanical bath, leads to
nonreciprocal transport of photons with 35dB of isolation. Additionally,
optical pumping with blue-detuned light manifests as a particle non-conserving
interaction between photons and phonons, resulting in directional optical
amplification of 12dB in the isolator through direction. These results indicate
the feasibility of utilizing optomechanical circuits to create a more general
class of nonreciprocal optical devices, and further, to enable novel
topological phases for both light and sound on a microchip.Comment: 18 pages, 8 figures, 4 appendice
Roles of cysteines Cys115 and Cys201 in the assembly and thermostability of grouper betanodavirus particles
The virus-like particle (VLP) assembled from capsid subunits of the dragon grouper nervous necrosis virus (DGNNV) is very similar to its native TÂ =Â 3 virion. In order to investigate the effects of four cysteine residues in the capsid polypeptide on the assembly/dissociation pathways of DGNNV virions, we recombinantly cloned mutant VLPs by mutating each cysteine to destroy the specific disulfide linkage as compared with thiol reduction to destroy all SâS bonds. The mutant VLPs of C187A and C331A mutations were similar to wild-type VLPs (WT-VLPs); hence, the effects of Cys187 and Cys331 on the particle formation and thermostability were presumably negligible. Electron microscopy showed that either C115A or C201A mutation disrupted de novo VLP formation significantly. As shown in micrographs and thermal decay curves, ÎČ-mercaptoethanol-treated WT-VLPs remained intact, merely resulting in lower tolerance to thermal disruption than native WT-VLPs. This thiol reduction broke disulfide linkages inside the pre-fabricated VLPs, but it did not disrupt the appearance of icosahedrons. Small dissociated capsomers from EGTA-treated VLPs were able to reassemble back to icosahedrons in the presence of calcium ions, but additional treatment with ÎČ-mercaptoethanol during EGTA dissociation resulted in inability of the capsomers to reassemble into the icosahedral form. These results indicated that Cys115 and Cys201 were essential for capsid formation of DGNNV icosahedron structure in de novo assembly and reassembly pathways, as well as for the thermal stability of pre-fabricated particles
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