Molecular cloning and preliminary function study of iron responsive element binding protein 1 gene from cypermethrin-resistant Culex pipiens pallens

<p>Abstract</p> <p>Background</p> <p>Insecticide resistance jeopardizes the control of mosquito populations and mosquito-borne disease control, which creates a major public health concern. Two-dimensional electrophoresis identified one protein segment with high sequence homology to part of <it>Aedes aegypti </it>iron-responsive element binding protein (IRE-BP).</p> <p>Method</p> <p>RT-PCR and RACE (rapid amplification of cDNA end) were used to clone a cDNA encoding full length IRE-BP 1. Real-time quantitative RT-PCR was used to evaluate the transcriptional level changes in the Cr-IRE strain <it>Aedes aegypti </it>compared to the susceptible strain of <it>Cx. pipiens pallens</it>. The expression profile of the gene was established in the mosquito life cycle. Methyl tritiated thymidine (³H-TdR) was used to observe the cypermethrin resistance changes in C6/36 cells containing the stably transfected IRE-BP 1 gene of <it>Cx. pipiens pallens</it>.</p> <p>Results</p> <p>The complete sequence of iron responsive element binding protein 1 (IRE-BP 1) has been cloned from the cypermethrin-resistant strain of <it>Culex pipiens pallens </it>(Cr-IRE strain). Quantitative RT-PCR analysis indicated that the IRE-BP 1 transcription level was 6.7 times higher in the Cr-IRE strain than in the susceptible strain of 4th instar larvae. The IRE-BP 1 expression was also found to be consistently higher throughout the life cycle of the Cr-IRE strain. A protein of predicted size 109.4 kDa has been detected by Western blotting in IRE-BP 1-transfected mosquito C6/36 cells. These IRE-BP 1-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by ³H-TdR incorporation.</p> <p>Conclusion</p> <p>IRE-BP 1 is expressed at higher levels in the Cr-IRE strain, and may confer some insecticide resistance in <it>Cx. pipiens pallens</it>.</p

Integrative healthcare approaches for children with special needs: a holistic study on challenges and solutions

This study delves into the intricate healthcare landscape for children with special needs, shedding light on the multifarious challenges these individuals face and evaluating the effectiveness of integrative healthcare models tailored to their unique requirements. Conducted at Sekolah Kebangsaan Pendidikan Khas Kuantan, this cross-sectional qualitative analysis involved detailed surveys with 20 teachers and engaging discussions with 24 students, aiming to unravel the complexities of providing holistic care to this vulnerable population. The investigation meticulously identifies pivotal concerns such as the prevalence of behavioral issues, particularly in autistic children, who often exhibit aggressive and self-injurious behaviors, and the struggles encountered by hearing-impaired students in communication and learning. It further explores the susceptibility of these children to frequent infections due to immune system dysfunction, exacerbated by dietary imbalances and physical inactivity. The research emphasizes the indispensable role of early detection and intervention in mitigating health risks and outlines the significance of specialized supplements, medications, and rigorous oral hygiene practices in maintaining the overall well-being of these children. Additionally, the study brings to the forefront the critical impact of socioeconomic factors on healthcare access, advocating for equitable healthcare solutions. It also highlights the transformative potential of technology, including telehealth and assistive devices, in overcoming barriers to healthcare and education. Through this analysis, the study advocates for a comprehensive, integrative care approach that encompasses medical, educational, social, and psychological services, underscoring the necessity of collaborative efforts among healthcare providers, educators, and families to significantly enhance the quality of life and well-being of children with special needs

Assesing Critical Length Of Dry Speel For Crop Growth Based On Soil Water Balance At Pengalenganpengkajian Deret Hari Kering Kritis Berdasarkan Neraca Air di Pengalengan

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Toxicity persistence in runoff and soil from experimental soybean plots following insecticide applications

Persistence of toxicity in runoff water and soil was investigated in experimental soybean plots subjected to successive runoff events following pesticide application. Runoff events were produced by irrigation using a sprinkler system. The pesticides applied were cypermethrin and endosulfan, which are widely used in soy production in Argentina. Toxicity tests were performed on two abundant components of the regional fauna, the amphipod Hyalella curvispina and the fish Cnesterodon decemmaculatus. Runoffs from two pesticide applications were assayed at different stages of the growing season: an early application when the soil was almost bare and a late one close to harvest, when the ground was covered by vegetation and just before soy leaves fell. Toxicity to H. curvispina in runoff ceased almost one month after the early application of the two pesticides, while it persisted for over three months after the late application. Soil toxicity to H. curvispina and runoff toxicity to C. decemmaculatus followed the same pattern. Higher temperatures and solar radiation are likely to have enhanced insecticide degradation after the early application. Lower temperatures and solar radiation in combination with increased organic matter from litter probably contributed to the longer persistence of toxicity recorded after the late application, as compared with the early application. Cypermethrin caused no mortality to C. decemmaculatus after the early application, while endosulfan toxicity persisted for almost four months after the late one