Mapping interactions with the chaperone network reveals factors that protect against tau aggregation.

A network of molecular chaperones is known to bind proteins ('clients') and balance their folding, function and turnover. However, it is often unclear which chaperones are critical for selective recognition of individual clients. It is also not clear why these key chaperones might fail in protein-aggregation diseases. Here, we utilized human microtubule-associated protein tau (MAPT or tau) as a model client to survey interactions between ~30 purified chaperones and ~20 disease-associated tau variants (~600 combinations). From this large-scale analysis, we identified human DnaJA2 as an unexpected, but potent, inhibitor of tau aggregation. DnaJA2 levels were correlated with tau pathology in human brains, supporting the idea that it is an important regulator of tau homeostasis. Of note, we found that some disease-associated tau variants were relatively immune to interactions with chaperones, suggesting a model in which avoiding physical recognition by chaperone networks may contribute to disease

Using PBIS to Create a Brand Identity and Learning Climate

This presentation will share how one high school in a medium size urban district leveraged PBIS to inculcate an improved environment, drastically reducing (by 75%) the number of Office Discipline Referrals (ODR), Out of School Suspensions (OSS), and tribunals over a three year period. The school implemented PBIS by making it a pervasive part of all aspects of the school, creating procedures and routines universally understood by all stakeholders. Also, an online tracking and management system was created to manage Level I and Level II offenses (those offenses not referred to Administration). Counseling was utilized to redirect students identified as struggling with Level I and Level II offenses. A rewards system was created that placed greater emphasis on intrinsic rewards rather than extrinsic rewards. In successfully implementing PBIS, a positive brand identity was created. Steps taken to re-brand the school will be discussed