Conceptualizing sexual and gender-based violence in European asylum reception centers

BACKGROUND: Sexual and gender based violence (SGBV) is a major public health problem and a violation of human rights. Refugees, asylum seekers and migrants are exposed to a constant risk for both victimization and perpetration. Yet, in the context of European asylum reception centers (EARF) professionals are also considered to be at risk. Our study explores the conceptualization of SGBV that residents and professionals have in this specific context. Further, we intent to identify key socio-demographic characteristics that are associated with SGBV conceptualization for both groups. METHODS: We developed a cross-sectional study using the Senperforto project database. Semi-structured interviews were conducted with residents (n = 398) and professionals (n = 202) at EARF. A principal component analysis (PCA) was conducted to variables related with knowledge on SGBV. Chi-square test and Fisher's exact test were applied to understand if significant statistical association exists with socio-demographic characteristics (significant level 0.5%). RESULTS: The majority of residents were male (64.6%), aged from 19 to 29 years (41.4%) and single (66.8%); for professionals the majority were women (56.2%), aged from 30 to 39 years (42.3%) and married (56.8%). PCA for residents resulted in 14 dimensions of SGBV representing 83.56% of the total variance of the data, while for professionals it resulted in 17 dimensions that represent 86.92% of the total variance of the data. For both groups differences in SGBV conceptualization were found according to host country, sex, age and marital status. Specific for residents we found differences according to the time of arrival to Europe/host country and type of accommodation, while for professionals differences were found according to legal status and education skills. CONCLUSION: Residents and professionals described different conceptualization of SGBV, with specific types of SGBV not being recognized as a violent act. Primary preventive strategies in EARF should focus on reducing SGBV conceptualization discrepancies, taking into account socio-demographic characteristics.publishersversionpublishe

Allosteric activation of Hsp70 reduces mutant huntingtin levels, the clustering of N-terminal fragments, and their nuclear accumulation

AIMS: Huntington's disease (HD) is caused by a mutant huntingtin protein that misfolds, yields toxic N-terminal fragments, aggregates, and disrupts proteostasis. The Hsp70 chaperone is a potential therapeutic target as it prevents proteotoxicity by favouring protein folding, disaggregation, or degradation. We tested the hypothesis that allosteric Hsp70 activation with a pharmacological mimetic of the Hsp70 co-chaperone Hip, YM-1, could modulate huntingtin proteostasis. MAIN METHODS: We used HD cell models expressing either N-terminal or full-length huntingtin. Using single-cell analysis we studied huntingtin aggregation in different cellular compartments by fluorescence microscopy. Protein interaction was evaluated by immunoprecipitation, while protein levels were quantified by immunofluorescence and western-blot. KEY FINDINGS: N-terminal huntingtin interacted with Hsp70 and increased its levels. Treatment with YM-1 reduced N-terminal huntingtin clustering and nuclear aggregation. Full-length mutant huntingtin also interacted with Hsp70, and treatment with YM-1 reduced huntingtin levels when combined with Hsp70 induction by heat shock. Mechanistically, YM-1 increases the Hsp70 affinity for substrates, promoting their proteasomal degradation. Consistently, YM-1 reduced the levels of ubiquitinated proteins. Interestingly, YM-1 accumulated in mitochondria, interfered with its Hsp70 isoform involved in protein import, and increased NRF1 levels, a regulator of proteasome genes. We thus suggest that YM-1 may trigger the coordination of mitochondrial and cytosolic proteostasis, enhancing protein degradation. SIGNIFICANCE: Our findings show that the strategy of allosteric Hsp70 activation holds potential for HD. While drug efficacy may be limited to tissues with elevated Hsp70, combined therapies with Hsp70 elevating strategies could harness the full potential of allosteric Hsp70 activators for HD

HIV, HBV and syphilis screening in antenatal care in Lubango, Angola.

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Robust canonical correlations: A comparative study.

Several approaches for robust canonical correlation analysis will be presented and discussed. A first method is based on the definition of canonical correlation analysis as looking for linear combinations of two sets of variables having maximal (robust) correlation. A second method is based on alternating robust regressions. These methods axe discussed in detail and compared with the more traditional approach to robust canonical correlation via covariance matrix estimates. A simulation study compares the performance of the different estimators under several kinds of sampling schemes. Robustness is studied as well by breakdown plots.

Ocular sequelae of congenital toxoplasmosis in Brazil compared with Europe

Toxoplasmic retinochoroiditis appears to be more severe in Brazil, where it is a leading cause of blindness, than in Europe, but direct comparisons are lacking. Evidence is accumulating that more virulent genotypes of Toxoplasma gondii predominate in South America

Strengthening of three-leaf stone masonry walls: an experimental research

The paper summarizes the results of an experimental research carried out on three-leaf masonry walls of typical granite stone constructions from the North of Portugal. The research aimed at studying the behaviour under compression of this wall typology, as well as the improvements introduced by common strengthening techniques applied for the structural rehabilitation of masonry heritage buildings. Ten masonry specimens were tested, plain or strengthened by transversal tying of the external leaves, with GFRP bars, or/and by injection of the inner leaf, with a lime-based grout. The results obtained showed that these strengthening techniques were successful in increasing the compressive strength of the walls and in improving their behaviour under compressive loads.The authors would like to thank the technical staff of the Structural Laboratory of University of Minho for the help provided. Acknowledgements are also due to the companies Fradical, Mapei and Augusto de Oliveira Ferreira for providing raw materials and workmanship. Finally, the funding provided by the Portuguese Science and Technology Foundation, through the POCI/ECM/58987/2004 project, is gratefully acknowledged

Cranial Masses in Sickle Cell Disease

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Mitochondrial dynamics and quality control in Huntington's disease

Huntington's disease (HD) is an inherited neurodegenerative disorder caused by polyglutamine expansion mutations in the huntingtin protein. Despite its ubiquitous distribution, expression of mutant huntingtin (mHtt) is particularly detrimental to medium spiny neurons within the striatum. Mitochondrial dysfunction has been associated with HD pathogenesis. Here we review the current evidence for mHtt-induced abnormalities in mitochondrial dynamics and quality control, with a particular focus on brain and neuronal data pertaining to striatal vulnerability. We address mHtt effects on mitochondrial biogenesis, protein import, complex assembly, fission and fusion, mitochondrial transport, and on the degradation of damaged mitochondria via autophagy (mitophagy). For an integrated perspective on potentially converging pathogenic mechanisms, we also address impaired autophagosomal transport and abnormal mHtt proteostasis in HD

Smallholder farmers challenges to supply institutional markets : a case study

Smallholder farmers face a number of difficulties in accessing the market, whether through local or informal sales, indirect sales through intermediaries or direct integration with the agribusiness. Purchases by the government through institutional markets emerged as a further recent distribution path. Access to institutional markets is an alternative that aims to increase income for smallholder farmers in situations of vulnerability in Brazil. While laws exist to encourage the participation to such markets by smallholder farmers, many of them still cannot access these markets. Therefore, the aim of this article is to identify the challenges faced by family farmers to supply products to the institutional markets. Findings show several factors have an impact to access the institutional markets, such as processes of participation in public calls; processes of production of items and challenges of delivering products

Evaluation of mutagenic/antimutagenic activity of conjugated linoleic acid in mice by micronucleus test

Conjugated linoleic acids (CLAs) are positional and geometrical isomers of linoleic acid and some researchers have shown biological activities including modulation of lipid metabolism, atherogenesis, diabetes, and immune functions. In this study, the animals were supplemented with 2% of the average feed consumption with CLA (G1 = positive control) and safflower oil (G2 = negative control) and the test groups were supplemented with CLA at the concentration of 2 and 4% of the average feed consumption. To assess the CLA potential protective effect, two groups were used; G3 = CLA 2% + cyclophosphamide (CP) and G5 = CLA 4% + CP. To assess the mutagenic effects of CLA, two groups were used; G4 = CLA 2% + NaCl 0.9% and G6 = CLA 4% + NaCl 0.9%. In order to investigate the mutagenic/antimutagenic effects of CLA, micronucleus test was used. The results showed variation of feed consumption in the groups that received 4% of CLA, when compared to the control group (G1 and G2) and CLA groups (G3 and G4) (p<0.05), during the period studied. It was observed that CLA did not show mutagenic effect at the concentrations tested (2 and 4%). Also, CLA showed antimutagenic effect at the same concentrations. However, the animals that received 4% of CLA, presented clinical signs of malnutrition.Key words: Conjugated linoleic acid, antimutagenicity, cyclophosphamide