Revised terminology of the Late Cambrian - Ordovician sequence of the Florentine - Denison Range area, and the significance of the "Junee Group"

The area of Lewis's original "Junee Series" is unsuitable as a basis for definitive stratigraphy and correlation,even with units in adjacent areas. A review of the various usages and concepts associated with the "Junee Group" indicates considerable diversity in meaning and application of the term, and suggests that the sequences are better considered in terms of a lower clastic unit and an upper limestone unit rather than as a single group. Accordingly, the Late Cambrian-Ordovician sequence in the Florentine Synclinorium is defined in terms of the Denison Subgroup, comprising four formations between the basal unconformity on the Denison Range and the base of the limestone, and the Gordon Subgroup, comprising three limestone formations and the Westfield Beds. These two Subgroups together approximate to the "Junee Group"

Ordovician Snatigraphy of the Florentine Synclinorium, south-west Tasmania

The Florentine Synclinorium consti tutes the type area of the Ordovician Junee Group in Tasmania, and the group is herein re-defined according to the formations present in this area. The base on the western side is formed by the Reeds Conglomerate, a unit of siliceous fanglomerate up to l,560 m thick lying conformably above a thick Upper Cambrian sequence on the Denison Range. The laterally-equivalent sandstone unit on the southeastern side is also given formation status (Tim Shea Sandstone). The overlying sequence of marine sandstone and siltstone is designated the Florentine Valley Formation, and is of Late Tremadocian-Arenigian age. A sub-unit of siltstone and limestone occurs in the middle part of the formation in some areas, but is not given formal status pending further mapping. The "Gordon Limestone", subdivided into three formations, becomes the Gordon Limestone Sub-Group. The basal Karmberg Limestone, of Upper Canadian -? Chazyan age, includes a mappable chert-rich unit which forms chert- covered ridges and is designated Wherretts Chert Member. The Cashions Creek Limestone, corresponding to the “Maclurites-Girvanella zone" of earlier reports, succeeds the Karmberg Limestone. Above this, and forming the bulk of the sequence, is the Benjamin Limestone, consisting of three members, viz. Lower Limestone Member, Lords Siltstone Member, Upper Limestone Member. A characteristic coral fauna with Favosites and cateniporines occurs near the top of the latter member, and includes conodonts which suggest an age not younger than Maysvillian. Above the limestone sequence and transitional with the overlying Eldon Group sandstone is a unit of siltstone and fine sandstone designated Westfield Beds. These contain a fauna correlated with the Richmondian, and the fauna in the overlying sandstone also appears to be Late Ordovician

Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I

Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred to as meiosis I and II). While sister kinetochores bind to microtubules emanating from opposite spindle poles during mitosis, they bind to microtubules originating from the same spindle pole during meiosis I. This phenomenon is referred to as mono-orientation and is essential for setting up the reductional mode of chromosome segregation during meiosis I. In budding yeast, mono-orientation depends on a four component protein complex referred to as monopolin which consists of two nucleolar proteins Csm1 and Lrs4, meiosis-specific protein Mam1 of unknown function and casein kinase Hrr25. Monopolin complex binds to kinetochores during meiosis I and prevents bipolar attachments. Although monopolin associates with kinetochores during meiosis I, its binding site(s) on the kinetochore is not known and its mechanism of action has not been established. By carrying out an imaging-based screen we have found that the MIND complex, a component of the central kinetochore, is required for monopolin association with kinetochores during meiosis. Furthermore, we demonstrate that interaction of monopolin subunit Csm1 with the N-terminal domain of MIND complex subunit Dsn1, is essential for both the association of monopolin with kinetochores and for monopolar attachment of sister kinetochores during meiosis I. As such this provides the first functional evidence for a monopolin-binding site at the kinetochore

Disease prevention not decolonization – a model for fecal microbiota transplantation in patients colonized with multidrug-resistant organisms

Fecal microbiota transplantation (FMT) yields variable intestinal decolonization results for multidrug-resistant organisms (MDROs). This study showed significant reductions in antibiotic duration, bacteremia and length of stay in 20 patients colonized/ infected with MDRO receiving FMT (compared to pre-FMT history, and a matched group not receiving FMT), despite modest decolonization rates

A Knowledge Graph Enhanced Learner Model to Predict Outcomes to Questions in the Medical Field

International audienceThe training curriculum for medical doctors requires the intensive and rapid assimilation of a lot of knowledge. To help medical students optimize their learning path, the SIDES 3.0 national French project aims to extend an existing platform with intelligent learning services. This platform contains a large number of annotated learning resources, from training and evaluation questions to students' learning traces, available as an RDF knowledge graph. In order for the platform to provide personalized learning services, the knowledge and skills progressively acquired by students on each subject should be taken into account when choosing the training and evaluation questions to be presented to them, in the form of customized quizzes. To achieve such recommendation , a first step lies in the ability to predict the outcome of students when answering questions (success or failure). With this objective in mind, in this paper we propose a model of the students' learning on the SIDES platform, able to make such predictions. The model extends a state-of-the-art approach to fit the specificity of medical data, and to take into account additional knowledge extracted from the OntoSIDES knowledge graph in the form of graph embeddings. Through an evaluation based on learning traces for pediatrics and cardiovascular specialties, we show that considering the vector representations of answers, questions and students nodes substantially improves the prediction results compared to baseline models

Variation in RNA expression and genomic DNA content acquired during cell culture

Specific chromosomal abnormalities are increasingly recognised to be associated with particular tumour subtypes. These cytogenetic abnormalities define the sites of specific genes, the alteration of which is implicated in the neoplastic process. We used comparative genomic hybridisation (CGH) to examine DNA from different breast and ovarian cancer cell lines for variations in DNA sequence copy number compared with the same normal control. We also compared different sources of the MCF7 breast line by both CGH and cDNA expression arrays. Some of the differences between the subcultures were extensive and involved large regions of the chromosome. Differences between the four subcultures were observed for gains of 2q, 5p, 5q, 6q, 7p, 7q, 9q, 10p, 11q, 13q, 14c, 16q, 18p and 20p, and losses of 4q, 5p, 5q, 6q, 7q, 8p, 11p, 11q, 12q, 13q, 15q, 19p, 19q, 20p, 21q, 22q and Xp. However, few variations were found between two subcultures examined, 5 months apart, from the same initial source. The RNA arrays also demonstrated considerable variation between the three different subcultures, with only 43% of genes expressed at the same levels in all three. Moreover, the patterns of the expressed genes did not always reflect our observed CGH aberrations. These results demonstrate extensive genomic instability and variation in RNA expression during subculture and provide supportive data for evidence that cell lines do evolve in culture, thereby weakening the direct relevance of such cultures as models of human cancer. This work also reinforces the concern that comparisons of published analyses of cultures of the same name may be dangerous

Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer

Background Mesothelin has attracted much interest as a tumor specific antigen; it has been reported to promote tumor development and to be a good target for cancer treatment. Most studies to date have used human mesothelin in immunocompromised mice. Since these models do not allow for study of the natural immune response to mesothelin expressing tumors, we have undertaken the characterization of mouse mesothelin so the effects of this protein can be assessed in immunocompetent mouse strains. Methods We analyzed mouse mesothelin expression, tissue distribution, shedding and biochemistry. In addition we constructed stable mesothelin overexpressing lines of the pancreatic cancer line Panc02 by two methods and tested them for growth and tumorigencity in vitro and in vivo. Results We show here that mouse mesothelin is similar to human mesothelin in biochemical characteristics, tumor expression and tissue distribution, suggesting the mouse may be a suitable model for study of mesothelin. Stable overexpression of mesothelin in a pancreatic cancer cell line did not increase cell proliferation or anchorage-independent growth in vitro, suggesting that mesothelin is not necessarily a tumor progression factor. Surprisingly overexpression of mesothelin inhibited tumor formation in vivo in immunocompetent mice. Conclusion The mouse may be a good model for studying mesothelin in the context of an intact immune response. Mesothelin is not necessarily a tumor progression factor, and indeed mesothelin overexpression inhibited tumor growth in immunocompetent mice

Unofficial policy: access to housing, housing information and social services among homeless drug users in Hartford, Connecticut

BACKGROUND: Much research has shown that the homeless have higher rates of substance abuse problems than housed populations and that substance abuse increases individuals' vulnerability to homelessness. However, the effects of housing policies on drug users' access to housing have been understudied to date. This paper will look at the "unofficial" housing policies that affect drug users' access to housing. METHODS: Qualitative interviews were conducted with 65 active users of heroin and cocaine at baseline, 3 and 6 months. Participants were purposively sampled to reflect a variety of housing statuses including homeless on the streets, in shelters, "doubled-up" with family or friends, or permanently housed in subsidized, unsubsidized or supportive housing. Key informant interviews and two focus group interviews were conducted with 15 housing caseworkers. Data were analyzed to explore the processes by which drug users receive information about different housing subsidies and welfare benefits, and their experiences in applying for these. RESULTS: A number of unofficial policy mechanisms limit drug users' access to housing, information and services, including limited outreach to non-shelter using homeless regarding housing programs, service provider priorities, and service provider discretion in processing applications and providing services. CONCLUSION: Unofficial policy, i.e. the mechanisms used by caseworkers to ration scarce housing resources, is as important as official housing policies in limiting drug users' access to housing. Drug users' descriptions of their experiences working with caseworkers to obtain permanent, affordable housing, provide insights as to how access to supportive and subsidized housing can be improved for this population

Using the Autism-Spectrum Quotient to Discriminate Autism Spectrum Disorder from ADHD in Adult Patients With and Without Comorbid Substance Use Disorder

It is unknown whether the Autism-spectrum quotient (AQ) can discriminate between Autism Spectrum Disorder (ASD) and Attention Deficit and Hyperactivity Disorder (ADHD) with or without comorbid Substance Use Disorder (SUD). ANOVA’s were used to analyse the mean AQ (sub)scores of 129 adults with ASD or ADHD. We applied receiver operating characteristic (ROC) computations to assess discriminant power. All but one of the mean AQ (sub)scores were significantly higher for adults with ASD compared to those with ADHD. The SUD status in general was not significantly associated with AQ (sub)scores. On the Social Skills subscale patients with ASD and comorbid SUD showed less impairment than those without SUD. The cut-off score 26 yielded 73% correct classifications. The clinical use of the AQ in differentiating between ASD and ADHD is limited