Kamishoyosan and Shakuyakukanzoto promote recovery from paclitaxel-induced neurite retraction in PC12 cells

Background: In chemotherapy, the full round of treatment must be completed as scheduled to achieve the strongest therapeutic effect. However, peripheral neuropathy, a severe side effect of the chemotherapeutic agent paclitaxel, can force the premature discontinuation of treatment. As some kampo practitioners have suggested that it may be possible to counteract such side effects, we analyzed the effects of Kamishoyosan, Shakuyakukanzoto, and Goshajinkigan in an in vitro model of paclitaxel-induced peripheral neuropathy. Methods: Paclitaxel-treated PC12 cells were assessed for neurite length and performed Western blot analysis for growth-associated protein-43 (GAP-43) and light neurofilament protein (NF-L) levels in the presence of nerve growth factor (NGF); they were re-assessed, with additional testing for acetylcholinesterase levels, after application of one of the kampo. We also compared phosphorylation of extracellular signal-regulated kinase (Erk)1/2 and Akt via Western blot analysis. About effect of kampo to anticancer efficacy, we confirmed cell cytotoxicity in A549 cells using MTT assay. Results: Addition of Kamishoyosan or Shakuyakukanzoto, but not Goshajinkigan, significantly improved neurite length and GAP-43 and NF-L levels from paclitaxel-treated PC12 cells, relative to those of only NGF-treated PC12 cells. The promoting effect of Kamishoyosan and Shakuyakukanzoto in neurite outgrowth is confirmed when NGF promoted neurite outgrowth, and it was inhibited partially when Erk1/2 and Akt were blocked by Erk1/2 inhibitor or Akt inhibitor alone. Furthermore, neurite outgrowth induced by TJ24 and TJ68 was inhibited more strongly when Erk1/2 inhibitor and Akt inhibitor were treated at the same time. NGF with Kamishoyosan or Shakuyakukanzoto promoted the proportion of phosphorylated Erk1/2 and phosphorylated Akt compare with NGF only. On the other hand, Kamishoyosan or Shakuyakukanzoto didn’t influence cytotoxicity of paclitaxel in A549 cells. Conclusions: Kamishoyosan or Shakuyakukanzoto promotes neurite outgrowth with NGF via increasing the proportion of phosphorylated Erk1/2 and phosphorylated Akt in PC12 cells. The effect applies to recovery from paclitaxel-induced axonal involvement and might promote recovery from paclitaxel-induced neuropathy without influence of anticancer effect of paclitaxel

ASK1-dependent recruitment and activation of macrophages induce hair growth in skin wounds

Apoptosis signal-regulating kinase 1 (ASK1) is a member of the mitogen-activated protein 3-kinase family that activates both c-Jun NH2-terminal kinase and p38 pathways in response to inflammatory cytokines and physicochemical stress. We report that ASK1 deficiency in mice results in dramatic retardation of wounding-induced hair regrowth in skin. Oligonucleotide microarray analysis revealed that expression of several chemotactic and activating factors for macrophages, as well as several macrophage-specific marker genes, was reduced in the skin wound area of ASK1-deficient mice. Intracutaneous transplantation of cytokine-activated bone marrow-derived macrophages strongly induced hair growth in both wild-type and ASK1-deficient mice. These findings indicate that ASK1 is required for wounding-induced infiltration and activation of macrophages, which play central roles in inflammation-dependent hair regrowth in skin

Two-Dimensional Dynamic Fusion for Continuous Authentication

Continuous authentication has been widely studied to provide high security and usability for mobile devices by continuously monitoring and authenticating users. Recent studies adopt multibiometric fusion for continuous authentication to provide high accuracy even when some of captured biometric data are of a low quality. However, existing continuous fusion approaches are resource-heavy as they rely on all classifiers being activated all the time and may not be suitable for mobile devices. In this paper, we propose a new approach to multibiometric continuous authentication: two-dimensional dynamic fusion. Our key insight is that multibiometric continuous authentication calculates two-dimensional matching scores over classifiers and over time. Based on this, we dynamically select a set of classifiers based on the context in which authentication is taking place, and fuse matching scores by multi-classifier fusion and multi-sample fusion. Through experimental evaluation, we show that our approach provides a better balance between resource usage and accuracy than the existing fusion methods. In particular, we show that our approach provides higher accuracy than the existing methods with the same number of score calculations by adopting multi-sample fusion.Comment: Accepted to IJCB'2

High-Grade Renal MTSCC

Mucinous tubular and spindle cell carcinoma (MTSCC) is a rare subtype of renal cell carcinoma. Although usually indolent, high-grade MTSCC has been reported to exhibit an aggressive clinical course. Herein, we report a case of high-grade renal MTSCC. An 86-year-old man visited our hospital with fever and fatigue. Based on contrast-enhanced computed tomography findings, the patient was diagnosed with clinical stage T2aN0M0 right renal cell carcinoma and underwent laparoscopic radical nephrectomy. Histological examination showed tubular to tubulopapillary structures accompanied by mucinous stroma, suggesting high-grade renal MTSCC. He remained recurrence- and metastasis-free 6 months after nephrectomy. Since high-grade renal MTSCC may have an aggressive clinical course, such patients should be observed carefully after radical nephrectomy

Movement patterns of the functional reach test do not reflect physical function in healthy young and older participants

東京都立大学Tokyo Metropolitan University博士（理学療法学）doctoral thesi

Underground behavior of overwintering Tokyo daruma pond frogs in early spring

Although Tokyo daruma pond frogs (Pelophylax porosus porosus) were once commonly observed throughout paddy fields in Japan, their populations have recently declined. The mode by which frogs survive during the overwintering period is largely unknown. In this study, we observed the underground behavior of 12 free-living Tokyo daruma pond frogs that overwintered in paddy soil and a dry field by periodically excavating their overwintering positions from late March to early May. The mean overwintering depth of these frogs was similar to 19.8 cm, with 10 having migrated toward the soil surface (mean depth: similar to 8.6 cm) by late March, and finally, all 11 live frogs migrated further upward close to the soil surface. In addition, males tended to emerge earlier in spring than females. One monitored frog in the paddy soil that migrated close to the soil surface died, presumably from plowing. Aside from the 12 study frogs, several other mutilated frog corpses were found in the paddies. This species starts migrating to the soil surface during the same period when human disturbances begin, making the frogs vulnerable to these disturbances during this period. Consequently, it is necessary to facilitate the emergence of frogs to protect them from human disturbances-e.g., by temporarily filling fields with water