70 research outputs found
Binding Site Alteration Is Responsible for Field-Isolated Resistance to Bacillus thuringiensis Cry2A Insecticidal Proteins in Two Helicoverpa Species
- Author
- AH Sayyed
- AH Sayyed
- AH Sayyed
- BE Tabashnik
- BE Tabashnik
- BE Tabashnik
- BE Tabashnik
- BE Tabashnik
- BE Tabashnik
- BE Tabashnik
- C James
- Carmen Sara HernĂĄndez-RodrĂguez
- CS HernĂĄndez
- CS HernĂĄndez-RodrĂguez
- D Convents
- DJ Wright
- E Schnepf
- EW Nester
- F Gould
- F Granero
- Haibing Yang
- J Ferré
- J Ferré
- J Ferré
- J GonzĂĄlez-Cabrera
- J Van Rie
- J Van Rie
- J Van Rie
- JBJ Van Rensburg
- Jeroen Van Rie
- JL Jurat-Fuentes
- JL Jurat-Fuentes
- Juan Ferré
- L English
- LJ Bird
- LJ Gahan
- MG Wolfersberger
- MI Ali
- MK Lee
- MM Bradford
- N Crickmore
- Nadine Bautsoens
- P Munson
- P Wang
- RJ Akhurst
- RJ Mahon
- RJ Mahon
- RJ Mahon
- Rod J. Mahon
- RT Roush
- S Downes
- S Downes
- S Herrero
- S Luo
- S Morin
- Sharon Downes
- Silvia Caccia
- SR Matten
- SW Baxter
- SW Baxter
- W Moar
- William James
- Y Gao
- Y Yang
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2010
- Field of study
Background
Evolution of resistance by target pests is the main threat to the long-term efficacy of crops expressing Bacillus thuringiensis (Bt) insecticidal proteins. Cry2 proteins play a pivotal role in current Bt spray formulations and transgenic crops and they complement Cry1A proteins because of their different mode of action. Their presence is critical in the control of those lepidopteran species, such as Helicoverpa spp., which are not highly susceptible to Cry1A proteins. In Australia, a transgenic variety of cotton expressing Cry1Ac and Cry2Ab (Bollgard II) comprises at least 80% of the total cotton area. Prior to the widespread adoption of Bollgard II, the frequency of alleles conferring resistance to Cry2Ab in field populations of Helicoverpa armigera and Helicoverpa punctigera was significantly higher than anticipated. Colonies established from survivors of F2 screens against Cry2Ab are highly resistant to this toxin, but susceptible to Cry1Ac.
Methodology/Principal Findings
Bioassays performed with surface-treated artificial diet on neonates of H. armigera and H. punctigera showed that Cry2Ab resistant insects were cross-resistant to Cry2Ae while susceptible to Cry1Ab. Binding analyses with 125I-labeled Cry2Ab were performed with brush border membrane vesicles from midguts of Cry2Ab susceptible and resistant insects. The results of the binding analyses correlated with bioassay data and demonstrated that resistant insects exhibited greatly reduced binding of Cry2Ab toxin to midgut receptors, whereas no change in 125I-labeled-Cry1Ac binding was detected. As previously demonstrated for H. armigera, Cry2Ab binding sites in H. punctigera were shown to be shared by Cry2Ae, which explains why an alteration of the shared binding site would lead to cross-resistance between the two Cry2A toxins.
Conclusion/Significance
This is the first time that a mechanism of resistance to the Cry2 class of insecticidal proteins has been reported. Because we found the same mechanism of resistance in multiple strains representing several field populations, we conclude that target site alteration is the most likely means that field populations evolve resistance to Cry2 proteins in Helicoverpa spp. Our work also confirms the presence in the insect midgut of specific binding sites for this class of proteins. Characterizing the Cry2 receptors and their mutations that enable resistance could lead to the development of molecular tools to monitor resistance in the [email protected]; [email protected]
Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic
- Author
- 14 | COVIDSurg COllabOratIVe Tzovaras G. (General University Hospital of Larissa)
- Aawsaj Y.
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- Tebala G. D.
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- Teixeira M. (Centro Hospitalar do Tamega e Sousa)
- Tejero- Pintor F. J. (Hospital Universitario RĂo Hortega)
- Testa V. (Santa Croce E. Carle Hospital
- Tewari N. (University Hospitals Coventry and Warwickshire NHS Trusts)
- Tezas S. (Furness General Hospital)
- Thaha M.
- Thanapal M. R. (Hospital Kuala Lumpur)
- Theodosopoulos T.
- Theophilidou E. (Royal Derby Hospital)
- Thian A.
- Thomas A. (Royal Liverpool University Hospital)
- Thompson D. (Morriston Hospital Swansea)
- Thornhill M. (Dell Seton Medical Center at the University of Texas)
- Thrumurthy S. (Epsom and St Helier University Hospitals NHS Trust)
- Thulasiraman S. V.
- Timbrell S.
- Tirloni L.
- Titu S. (Prof. Dr. Ion Chiricuta Institute of Oncology). Russia: Garmanova T.
- Tonini V. (S. Orsola-Malpighi Hospital)
- Torres A. (Hospital ClĂnico de Madrid)
- Torres Arcos C.
- Townson A.
- Trabulsi N. (King Abdulaziz University Hospital)
- Travaglio E. (Regina Montis Regalis Hospital
- Tribillon E. (Institut Mutualiste Montsouris)
- Troncoso Pereira P. (Hospital Mateu Orfila)
- Tropeano F. P. (Federico II University Hospital)
- Trujillo DĂaz J.
- Tsai L.
- Tsarkov P.
- Tsiamalou I.
- Tsiaoussis I.
- TU Dresden)
- Tucci R. (Azienda Ospedaliera Universitaria Careggi)
- Tufo A. (Ospedale del Mare)
- Tugmen C.
- Tulina I. (Clinic of Coloproctology and Minimally Invasive Surgery
- Tuncer K.
- Turnbull A. (Victoria Hospital Kirkcaldy)
- Turrado-Rodriguez V.
- Turri G. (Azienda Ospedaliera Universitaria Integrata di Verona)
- Tweedle E. (Addenbrookeâs Hospital)
- Ugarte-Sierra B. (Hospital Universitario de Galdakao)
- UludaÄ S. S.
- Umair M.
- Universiti Sains Malaysia)
- University of Amsterdam)
- UniversitĂ di Ferrara)
- Université Mohammed V Rabat). Netherlands: Hompes R.
- Uras C. (Acibadem Maslak Hospital)
- Urbani A. (Azienda Ospedaliero Universitaria Sanâanna)
- Urbieta A. (Hospital Universitario la Paz)
- Uslu G.
- Utkan N. Z.
- Uyanik M. S. (Samsun Training and Research Hospital)
- Uzunoglu F. G. (University Medical Center HamburgâEppendorf). Greece: Antonakis P.
- Vaccarella G. (Azienda Ospedaliera Ospedali Riuniti Villa Sofia-Cervello)
- Valbuena Jabares V. (Marqués de Valdecilla University Hospital)
- Valdes- Hernandez J. (Hospital Universitario Virgen Macarena)
- Valero Soriano M. (Hospital General Reina SofĂa)
- Valle Rubio A. (Getafe University Hospital)
- Valverde S. (Hospital Clinic Barcelona)
- Van den Eynde J.
- Van den Eynde R. (Uz Leuven). Bulgaria: Sokolov M. (University Hospital Alexandrovska). Canada: Boutros M.
- Varma M. (University of California
- VasljeviÄ J.
- Vassiliu P. (Attikon University General Hospital)
- Vaz Pereira R.
- Vaz S. (Centro Hospitalar e UniversitĂĄrio de SĂŁo JoĂŁo)
- Velchuru V.
- Velickovic D. (Clinic for Digestive Surgery
- Velidedeoglu M.
- Venn M. (Royal London Hospital)
- Venskutonis D. (Lithuanian University of Health Sciences Kaunas Clinical Hospital). Madagascar: Rasoaherinomenjanahary F.
- Vera Mansilla C. (Hospital Universitario Principe de Asturias)
- Verberne C. (John Radcliffe Hospital)
- Vergari R. (Ospedali Riuniti di Ancona)
- Vernon L. (Cork University Hospital)
- Vervoort D.
- Vescio G. (University âMagna Graeciaâ of Catanzaro)
- Vezakis A. (Aretaieion Hospital)
- Videira J. F. (IPO Porto). RĂ©union: Kassir R.
- Vieira B. (Centro Hospitalar de TrĂĄs-Os-Montes e Alto Douro
- Vieira Caroço T. (IPO Coimbra)
- Vieira Paiva Lopes A. C. (Hospital de Torres Vedras â Centro Hospitalar do Oeste)
- Vignali A. (San Raffaele Scientific Institute
- Vigorita V. (Ălvaro Cunqueiro Hospital)
- Vijayan D. (Queen Elizabeth Hospital Birmingham)
- Villalabeitia Ateca I. (Hospital Universitario Cruces)
- Vimalachandran D. (Countess of Chester Hospital)
- Viswanath Y. K. S. (James Cook University Hospital)
- Vitali A. (Ospedale San Giovanni di Dio)
- Vitone L. (Royal Blackburn Hospital)
- Vives R. V. (Bellvitge University Hospital)
- Vo U. G. (Fiona Stanley Hospital)
- Von Papen M.
- Vovola F. (Ospedali Riuniti Azienda Ospedaliera Universitaria Foggia)
- Vucic N.
- VĂĄzquez FernĂĄndez A. (Hospital ClĂnico Universitario de Valladolid)
- Wadham B. (The University Hospital of South Manchester)
- Walkes K. (Queen Elizabeth Hospital). Belgium: Flamey N.
- Wani R. (Sher-I-Kashmir Institute of Medical Sciences)
- Waqar S. H. (Pakistan Institute of Medical Sciences). Poland: Major P. (Jagiellonian University Medical College). Portugal: Azevedo C.
- Warren O. (Chelsea and Westminster Hospital)
- Watson L. J. (Sunderland Royal Hospital)
- Watson N. (Kingâs Mill Hospital)
- Weitz J.
- Welsch T. (Carl-Gustav-Carus University Hospital
- Werner J. (LMU Klinikum Campus Innenstadt)
- Westwood E. (James Paget University NHS Foundation Trust Hospital)
- Whelan M. (Tallaght University Hospital)
- Whitmore H. (Torbay and South Devon NHS Trust)
- Wilkin R. J. W. (South Warwickshire NHS Foundation Trust)
- Wilkins A. (Hull University Teaching Hospitals NHS Trust)
- Williams G. (Royal Gwent Hospital)
- Williams K. (St Georgeâs Hospital)
- Wilson M. (Forth Valley Royal Hospital)
- Wimmer A. (Paracelsus Medical University Salzburg). Barbados: Barker D.
- Wong M.
- Worku D. (Queens Medical Centre)
- Wullschleger M. (Gold Coast University Hospital)
- Xavier R. G.
- Xenaki S.
- Xynos E. (University Hospital of Heraklion Crete and Interclinic Hospital of Crete). Hong Kong: Futaba K.
- Yang W.
- Yassin N. (Royal Wolverhampton NHS Trust)
- Ye G.
- Yesantharao P.
- Yetkin S. G. (Sisli Hamidiye Etfal Training and Research Hospital)
- YeĆilyurt D. (University of Health Sciences Tepecik Training and Research Hospital)
- Yiallourou A. (Nicosia General Hospital). Czechia: Martinek L.
- Yıldırım A.
- Yildiz A. (Yildirim Beyazit University Yenimahalle Training and Research Hospital). Uganda: Lule H.
- Young R. (York Teaching Hospitals NHS Trust)
- Yousof E. A. (Assiut University Hospital)
- Youssef M. (Norfolk and Norwich University Hospital)
- Yurttas C. (University Hospital Tuebingen)
- YĂĄnez C. (Royo Villanova)
- YĂŒksel E. (Kocaeli University Teaching Hospital)
- Zafar S. N. (University of Wisconsin)
- Zakaria A. D.
- Zakaria Z. (School of Medical Sciences and Hospital
- Zambon M. (Policlinico Umberto I Sapienza University of Rome)
- Zanus G. (Caâ Foncello Treviso â DISCOG â UniversitĂ di Padova)
- Zegarac M. (Institute for Oncology and Radiology of Serbia)
- ZeliÄ M. (University Hospital Center Rijeka)
- Zengin A. K. (Istanbul Universty â CerrahpaĆa Medical Faculty)
- Zerbib P. (CHU Lille)
- Zervas K.
- Zese M. (Santa Maria degli Angeli Hospital ULSS 5 â Adria)
- Zhang H. (Concord Repatriation General Hospital)
- Zizzo M. (Azienda UnitĂ Sanitaria Locale â IRCCS di Reggio Emilia)
- Zografos C. (Laiko University Hospital)
- Zorrilla OrtĂșzar J. (Hospital General Universitario Gregorio Marañón)
- Zourntou S. (General Hospital of Larissa âKoutlimpaneio and Triantafylleioâ)
- Zubaidi A. M. (King Saud University). Serbia: AleksiÄ L.
- Ăngelo M.
- Ăelebi F.
- Ăolak E.
- Ăfner D. (Medical University of Innsbruck)
- Ăhlberger L.
- Ăzçelık M. F.
- ĂÄĂŒcĂŒ H.
- ĂberrĂŒck L.
- Äoza I.
- Publication venue
- 'Wiley'
- Publication date
- 01/01/2020
- Field of study
This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic
Understanding the role of Bi2O3 in the P2O5âCaOâNa2OâK2O glass system in terms of physical, structural and radiation shielding properties
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Shielding effectiveness of boron-containing ores in Liaoning province of China against gamma rays and thermal neutrons
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
A comprehensive investigation on the role of PbO in the structural and radiation shielding attribute of P2O5âCaOâNa2OâK2OâPbO glass system
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
A comprehensive study on the optical, mechanical, and radiation shielding properties of the TeO2âLi2OâGeO2 glass system
- Author
- A Makishima
- A Makishima
- A Ramesh Babu
- AH Almuqrin
- AS Abouhaswa
- CB Deng
- DJ Bergman
- E Kavaz
- ES Yousef
- F Nawaz
- F Urbach
- G Yankov
- GA Saunders
- H Akyildirim
- HMM Moawad
- HN Desai
- J Tauc
- K Kirdsiri
- KA Mahmoud
- KM Kaky
- KM Kaky
- KM Kaky
- KM Kaky
- M Kumar
- M Kurudirek
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MK Narayanan
- MY Hanfi
- MY Hanfi
- N Elkhoshkhany
- NF Mott
- R El-Mallawany
- R Kurtulus
- R Mondal
- RJ Amjad
- S Hussain
- S Ruengsri
- S Stalin
- S Yasmin
- SAM Issa
- SF Khor
- SR Manohara
- V Dimitrov
- WE Mahmoud
- XY Zhang
- Y Al-Hadeethi
- Ă EyecioÄlu
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
The optimization of gelatin extraction from chicken feet and the development of gelatin based active packaging for the shelf-life extension of fresh grapes
- Publication venue
- Molecular Diversity Preservation International (MDPI)
- Publication date
- 18/09/2023
- Field of study
202309 bcvcVersion of RecordNot mentionPublishe
Stuctural, optical and radiation shielding properties of zinc boro-tellurite alumina glasses
- Author
- A Madhu
- Alyaa H. Abdalsalam
- AM Zoulfakar
- B Shan
- C Eke
- D Han
- D Singh
- D Souri
- F Akman
- F Akman
- F El-Diasty
- Farah Laariedh
- G Lakshminarayana
- H. O. Tekin
- HO Tekin
- J Bernier
- K Keshavamurthy
- K Maheshvaran
- K Maheshvaran
- K Maheshvaran
- K Selvaraju
- K Siva Rama
- Kawa M. Kaky
- KB Sapnar
- KM Kaky
- KM Kaky
- L Xing
- M Anand Pandarinath
- M Dogra
- M Dong
- M Kurudirek
- M Mariyappan
- M. I. Sayyed
- MHM Zaid
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MI Sayyed
- MS Gaafar
- N Kaur
- N Santha
- P Gayathri Pavani
- P Karthikeyan
- P Limkitjaroenporn
- P Yasaka
- R Bagheri
- R Khaldari
- RS Kundu
- S Rada
- S Rada
- S Yasmin
- S. O. Baki
- SS Obaid
- SS Science
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Structural, optical, and radiation shielding features for a series of borate glassy system modified by molybdenum oxide
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Effects of MgO addition on the radiation attenuation properties of 45S5 bioglass system at the energies of medical interest: an in silico study
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
- âŠ