Phytochemical screening of Saye, a traditional herbal remedy for malaria

phytochemical assay was conducted to establish the chemical profile of “Saye”, a mixture of leaf of Cassia alata, root of Cochlospermum planchonii and whole plant of Phyllantus amarus, used as antimarial remedy. Water and organic extracts were prepared. Characterization of phytoconstituents using specific chemical reagents was performed in tubes, by thin layer chromatography and by high performance liquid chromatography. Steroids and/or triterpenes, cathechic tannins were identified in the decocted and the macerated water extracts of “Saye”. An anthraquinone with a retention time Rt corresponding to 3.34 min was identified by the HPLC analysis.© 2015 International Formulae Group. All rights reserved.Keywords: Chemical profile, anthraquinones, steroids, triterpenes, tannins

The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins

Snakebite envenoming is a serious and neglected tropical disease that kills ~100,000 people annually. High-quality, genome-enabled comprehensive characterization of toxin genes will facilitate development of effective humanized recombinant antivenom. We report a de novo near-chromosomal genome assembly of Naja naja, the Indian cobra, a highly venomous, medically important snake. Our assembly has a scaffold N50 of 223.35 Mb, with 19 scaffolds containing 95% of the genome. Of the 23,248 predicted protein-coding genes, 12,346 venom-gland-expressed genes constitute the \u27venom-ome\u27 and this included 139 genes from 33 toxin families. Among the 139 toxin genes were 19 \u27venom-ome-specific toxins\u27 (VSTs) that showed venom-gland-specific expression, and these probably encode the minimal core venom effector proteins. Synthetic venom reconstituted through recombinant VST expression will aid in the rapid development of safe and effective synthetic antivenom. Additionally, our genome could serve as a reference for snake genomes, support evolutionary studies and enable venom-driven drug discovery

A systematic review showing the lack of diagnostic criteria and tools developed for lower-limb cellulitis

BACKGROUND: Cellulitis can be a difficult diagnosis to make. Furthermore, 31% of patients admitted from the emergency department with suspected lower-limb cellulitis have been misdiagnosed, with incorrect treatment potentially resulting in avoidable hospital admission and the prescription of unnecessary antibiotics. OBJECTIVES: We sought to identify diagnostic criteria or tools that have been developed for lower-limb cellulitis. METHODS: We conducted a systematic review using Ovid MEDLINE and Embase databases in May 2018, with the aim of describing diagnostic criteria and tools developed for lower-limb cellulitis, and we assessed the quality of the studies identified using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. We included all types of study that described diagnostic criteria or tools. RESULTS: Eight observational studies were included. Five studies examined biochemical markers, two studies assessed imaging and one study developed a diagnostic decision model. All eight studies were considered to have a high risk for bias in at least one domain. The quantity and quality of available data was low and results could not be pooled owing to the heterogeneity of the findings. CONCLUSIONS: There is a lack of high-quality publications describing criteria or tools for diagnosing lower-limb cellulitis. Future studies using prospective designs, validated in both primary and secondary care settings, are needed. What's already known about this topic? Diagnosing lower-limb cellulitis on first presentation is challenging. Approximately one in three patients admitted from the emergency department with suspected lower-limb cellulitis do not have cellulitis and are given another diagnosis on discharge. Consequently, this results in potentially avoidable hospital admissions and the prescription of unnecessary antibiotics. There are no diagnostic criteria available for lower-limb cellulitis in the U.K. What does this study add? This systematic review has identified a key research gap in the diagnosis of lower-limb cellulitis. There is a current lack of robustly developed and validated diagnostic criteria or tools for use in clinical practice

Seizure prediction : ready for a new era

Acknowledgements: The authors acknowledge colleagues in the international seizure prediction group for valuable discussions. L.K. acknowledges funding support from the National Health and Medical Research Council (APP1130468) and the James S. McDonnell Foundation (220020419) and acknowledges the contribution of Dean R. Freestone at the University of Melbourne, Australia, to the creation of Fig. 3.Peer reviewedPostprin

Multiscale investigations of nanoprecipitate nucleation, growth, and coarsening in annealed low-Cr oxide dispersion strengthened FeCrAl powder

A major challenge in the design of oxide dispersion strengthened (ODS) FeCrAl alloys is the optimization of the fine-scale particle size distribution that provides both beneficial mechanical properties and irradiation resistance. To address this obstacle, the nucleation, growth, and coarsening of the fine-scale (Y,Al,O) nanoprecipitates within an ODS FeCrAl powder was studied using atom probe tomography (APT) and small-angle neutron scattering (SANS). Mechanically alloyed Fe–10Cr-6.1Al-0.3Zr + Y2O3 wt.% (CrAZY) powders were heated in-situ from 20 to 1000 °C to capture the nucleation and growth of the nanoprecipitates using SANS. Furthermore, CrAZY powders were annealed at 1000 °C, 1050 °C, and 1100 °C for ageing times from 15 min to 500 h followed by either APT or magnetic SANS to study the structure, composition, and coarsening kinetics of the nanoprecipitates at high temperature. In-situ SANS results indicate nanoprecipitate nucleation and growth at low temperatures (200–600 °C). APT results revealed compositions corresponding to the cubic Y3Al5O12 garnet (YAG) stoichiometry with a possible transition towards the perovskite YAlO3 (YAP) phase for larger precipitates after sufficient thermal ageing. However, magnetic SANS results suggest a defective structure for the nanoprecipitates indicated by deviations of the calculated A-ratio from stoichiometric (Y,Al,O) phases. Particle coarsening kinetics follow n = 6 power law kinetics with respect to particle size, but the mechanism cannot be explained through the dislocation pipe diffusion mechanism. The potential effect of precipitate coarsening during pre- and post-consolidation heat treatments on the irradiation resistance of ODS FeCrAl alloys is discussed with respect to sink strength maximization

Phytochemical screening of Saye, a traditional herbal remedy for malaria

phytochemical assay was conducted to establish the chemical profile of “Saye”, a mixture of leaf of Cassia alata, root of Cochlospermum planchonii and whole plant of Phyllantus amarus, used as antimarial remedy. Water and organic extracts were prepared. Characterization of phytoconstituents using specific chemical reagents was performed in tubes, by thin layer chromatography and by high performance liquid chromatography. Steroids and/or triterpenes, cathechic tannins were identified in the decocted and the macerated water extracts of “Saye”. An anthraquinone with a retention time Rt corresponding to 3.34 min was identified by the HPLC analysis.© 2015 International Formulae Group. All rights reserved.Keywords: Chemical profile, anthraquinones, steroids, triterpenes, tannins

Enhancing EPC supply chain competitiveness through procurement risk management

This article is intended to provide a risk-based procurement management model. The Procurement Risk Cube (PRC) model originates a systematic approach in the identification of the case-specific risks, their sources, the activities on which they impact and the risk owners. The hierarchical breakdown structure helps data aggregation by simply summing the values of exposure by owner / activity / source, so that establishing a correct intervention priority plan to mitigate downside risks, or to exploit upside risks, becomes easier and more effective. A deep insight into case-specific procurement-related risks is provided by the PRC, which is necessary to improve the understanding of the risks in the supply chain (SC) in order to achieve benefit for the whole SC