189 research outputs found
Clinical and hematological findings in Leishmania braziliensis-infected dogs from Pernambuco, Brazil
Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi
Facilidades e dificuldades da famĂlia no cuidado Ă criança com paralisia cerebral
Pesquisa qualitativa, exploratĂłria, descritiva, que objetivou apreender as facilidades e as dificuldades da famĂlia nocuidado Ă s crianças com paralisia cerebral. Os dados foram coletados com sete famĂlias, entre março e abril de 2009,por meio de entrevista semiestruturada. A análise seguiu os princĂpios da interpretação temática. As dificuldades sĂŁomais evidenciadas que as facilidades e trazem repercussões nas atividades de vida diária. Lidar com convulsões dedifĂcil controle, espasticidade, alterações de tĂ´nus postural, presença de reflexos primitivos implicam na complexidadeem tarefas simples, como alimentação, banho, locomoção, pois as crianças ficam, cada dia, mais dependentes doscuidadores. Com o tempo, as dificuldades cedem lugar Ă rotina e Ă adaptação Ă vida diária da criança. Estreitarrelações com a famĂlia, melhorando o vĂnculo e desenvolvendo ações de qualidade e integrais, promovem o bemestarda famĂlia/criança com paralisia cerebral
Gender effect on clinical features of achalasia: a prospective study
BACKGROUND: Achalasia is a well-characterized esophageal motor disorder but the rarity of the disease limits performing large studies on its demographic and clinical features. METHODS: Prospectively, 213 achalasia patients (110 men and 103 women) were enrolled in the study. The diagnosis established by clinical, radiographic, and endoscopic as well as manometry criteria. All patients underwent a pre-designed clinical evaluation before and within 6 months after the treatment. RESULTS: Solid dysphagia was the most common clinical symptom in men and women. Chest pain was the only symptom which was significantly different between two groups and was more complained by women than men (70.9% vs. 54.5% P value= 0.03). Although the occurrence of chest pain significantly reduced after treatment in both groups (P < 0.001), it was still higher among women (32% vs. 20.9% P value= 0.04). In both sexes, chest pain did not relate to the symptom duration, LES pressure and type of treatment patients received. Also no significant relation was found between chest pain and other symptoms expressed by men and women before and after treatment. Chest pain was less frequently reported by patients over 56 yrs of age in comparison to those less than 56 yrs (p < 0.05). CONCLUSION: It seems that chest pain is the distinct symptom of achalasia which is affected by sex as well as age and does not relate to the duration of illness, LESP and the type of treatment achalasia patients receive
Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
Contribution of submandibular gland and swallowing structure sparing to post-radiation therapy PEG dependence in oropharynx cancer patients treated with split-neck IMRT technique
Seroepidemiological aspects ofLeishmania spp. in dogs in the Itaguai micro-region, Rio de Janeiro, Brazil
Hybrid Sandwich Particleboard Made with Sugarcane, PĂnus Taeda Thermally Treated and Malva Fibre from Amazon
Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs
Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis
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