Use of Lactobacillus farciminis to improve antioxidant status of Tuj lambs

The aim of this study was to investigate the effects of Lactobacillus farciminis on growth traits and antioxidant status in preweaning and postweaning Tuj lambs. Twenty lambs were divided into four groups, regardless of gender, with a mean live weight of 7.81 ± 0.50 kg. At the start of the experiment, the average age of the lambs was seven days. During the six-week preweaning period, control (C) lambs were fed with colostrum only, and Lactobacillus farciminis was given orally to the treated lambs at 1 g/day/lamb (L1), 2 g/day/lamb (L2) or 4 g/day/lamb (L3). The experiment continued for a total of 22 weeks. During the first six weeks, bodyweight (BW) increased significantly in L1 at the sixth week. Also during this period, bodyweight gain (BWG) in L2 at 2 - 3 weeks and in L3 at 5 - 6 weeks differed from C. In the subsequent period, BW and BWG were not affected by probiotic supplementation. The effects of probiotic supplementation on malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were significant throughout the experiment, with the effect on glutathione (GSH) also being important in the first six weeks. Thus, Lactobacillus farciminis provided orally to Tuj breed lambs could be used to improve their antioxidant status without compromising growth

Investigation of haptoglobin, serum amyloid A, and some biochemical parameters in calves with omphalitis

Aim: In this study, it was aimed to determine the concentration of some important acute phase proteins (APPs) and some biochemical parameters pre-operative and post-operative in calves with omphalitis. Materials and Methods: A total of 20 calves were used in the study and they consist of 10 clinically healthy calves that were used as a control and 10 calves with omphalitis were used as the treatment group. Blood samples were collected from Vena jugularis of animals to tubes with anticoagulant (sodium citrate) and without anticoagulants, pre-operative (day 0), and post-operative (day 7). Samples were used to determine the concentration of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), fibrinogen, glucose, total protein, albumin, urea, total bilirubin, creatinine, calcium, phosphorus, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) concentrations. Results: While the Hp, SAA, Cp, fibrinogen, urea, creatinine, total bilirubin, ALP, and GGT concentrations were statistically and significantly increasing rather than the control group during the pre-operative period for calves with omphalitis, they decreased to the post-operative period. Moreover, an insignificant increase in the glucose, total protein, and AST concentrations and an insignificant decrease in the albumin, calcium, and phosphorus concentrations were statistically determined. Conclusion: We have the opinion that the assessment of biochemical parameters and especially APP levels in calves with the omphalitis together with the clinical findings may be important in terms of the treatment and prognosis

Determination of ceruloplasmin, some other acute phase proteins, and biochemical parameters in cows with endometritis

Aim: The aim of this study is to determine serum ceruloplasmin levels in cows with endometritis of varying degrees of severity and to establish whether or not there is a correlation between acute phase protein (APP) levels and biochemical parameters. Material and Methods: The study was conducted with 100 Brown Swiss cows (3-8 years of age) on days 28-32 postpartum. Cows were divided into endometritis (mild, moderate, and severe endometriosis) and healthy groups based on ultrasonography, vaginoscopy, and cytological examination. Blood samples were collected from all cows. Levels of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin, albumin, and some biochemical parameters were analyzed. Results: Hp, SAA, and ceruloplasmin levels were higher in cows with endometritis than in healthy cows (p=0.001), and the levels of these APPs increased as endometritis became more severe (p=0.001). Some significant correlations were found between APPs and the biochemical parameters that were analyzed. In conclusion, it was determined that ceruloplasmin levels increase significantly in the presence of endometritis and proportionate to the severity of endometritis. A significant correlation was found between ceruloplasmin levels and Hp and SAA levels. Conclusion: It was concluded that ceruloplasmin levels can be used in the diagnosis of endometritis as an alternative to Hp and SAA levels

Effects of reduced glutathione on nitric oxide level, total antioxidant and oxidant capacity and adenosine deaminase activity

The aim of this study was to determine the effect of exogenous GSH, an important antioxidant containing thiol group, on total antioxidant capacity (TAC) and total oxidant capacity (TOC), adenosine deaminase (ADA), a significant indicator of cellular immunity, and nitric oxide (NO) concentrations in rabbits

Effects of estradiol, progesterone and kisspeptin-1 concentrations at the time of insemination on conception rates in cattle

The present study aimed to investigate the effects of kisspeptin (Kiss-1), estradiol (E2), and progesterone (P4) concentrations at the time of artificial insemination (AI) on conception rates and whether or not there is a relationship between Kiss-1 and E2 concentrations in cattle. Cows (n=100) raised on a semi-open free-stall barn and having corpus luteum were intramuscularly injected 25 mg of prostaglandin F2α (5 mg Dinoprost, Dinolytic®, Zoetis, Spain) and signs of estrus were checked for 5 days after injection. The cows that did not show heat for 5 days following the PGF2α injection were injected with the second dose of PGF2α on the 7th day and the oestrus was checked for half an hour, 3 times a day for 72 hours. Just prior to AI, preovulatory follicle diameters were examined with transrectal ultrasonography and recorded. Then blood samples were collected. Blood serum samples were collected and stored until analyzed. Pregnancies were diagnosed with transrectal real time B-Mod ultrasonography on day 30±2 following AI. The mean follicular diameters at the time of AI were 17.82±0.01 mm. E2, P4, and Kiss-1 concentrations at the time of AI were determined in serum by ELISA. Mean concentrations of Kiss-1, estradiol and progesterone at the time of AI in all animals were 79.77±4.64 pg/mL, 117.09±1.48 pg/mL, and 0.61±0.02 ng/mL, respectively. The threshold value for kisspeptin, estradiol and progesterone concentrations were determined to be 82.03 pg/mL, 116.22 pg/mL and 0.93 ng/mL, respectively. Kiss-1 is a positive and significant predictor for the model but has limited accuracy. While Kiss-1, follicle diameter, milk yield, lactation period and AI number explains high accuracy (AUC=0.738) in pregnancy prediction, estrogen and progesterone are poor. In addition, a significant correlation was found between serum kiss-1 concentration and follicle diameter, whereas no significant correlation was found between follicle diameter and serum estrogen or progesterone concentrations. In conclusion, Kiss-1 was found to be limited accurate in predicting pregnancy in cows, whereas estradiol and progesterone were not

The protective effect of betanin and copper on heart and lung in end-organ ischemia reperfusion injury

bilgi, zeynep/0000-0003-4981-047XWOS: 000528258200007PubMed: 32115979OBJECTIVES: Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats. METHODS: Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay. RESULTS: MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. in both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. the numbers of apoptotic cells were significantly decreased in both treatment groups. CONCLUSION: Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).Kafkas University BAPKafkas University [2017-TS-14]This work was supported by Kafkas University BAP #2017-TS-14