Frequência de conforto da temperatura e umidade em creches de suínos com programas de iluminação nos períodos de inverno e verão.

Projeto: 11.11.11.111

Características micromorfológicas de biótipos de capim-arroz resistente e suscetível ao quinclorac.

RESUMO - O objetivo deste trabalho foi avaliar o efeito do quinclorac nas características anatômicas de folhas e raízes de biótipos de capim‑arroz (Echinochloa spp.), resistente e suscetível a este herbicida, por meio de avaliações micromorfométricas. As plantas foram cultivadas em vasos de plástico com capacidade de 250 cm3. As imagens dos cortes foram obtidas com microscópio de luz equipado com sistema U‑Photo, acoplado à câmera digital conectada a microcomputador. Na seção transversal da lâmina foliar e das raízes, utilizouse objetiva de 10X, tendo-se avaliado 20 cortes/campos por planta, com dez medições por corte e quatro repetições por tratamento. Observaram-se diferenças entre os biótipos resistente e suscetível, tanto na ausência quanto na presença do quinclorac. O biótipo resistente apresenta lâmina foliar pouco afetada pelo herbicida; no entanto, há modificação na constituição dos tecidos radiculares com a formação mais acentuada de aerênquima. O biótipo suscetível também apresenta formação de aerênquima quando tratado com o quinclorac, mas em quantidade inferior à observada no biótipo resistente. ABSTRACT- The objective of this work was to evaluate the effect of quinclorac on anatomical traits of leaves and roots of barnyardgrass (Echinochloa spp.) biotypes, susceptible and resistant to this herbicide, by micromorphometric assessments. Plants were grown in plastic pots with 250 cm3 capacity. The images of plant sections were obtained with a light microscope equipped with a U‑Photo system, coupled to a digital photo camera connected to a microcomputer. For cross-sections of leaf blades and roots, 10X objectives were used, and 20 sections/fields per plant were evaluated, with ten measurements per section and four replicates per treatment. Differences were observed between resistant and susceptible biotypes, both in the absence and in the presence of quinclorac. The resistant biotype shows leaf blades that are less affected by the herbicide; however, there are changes in root tissue constitution with increased aerenchyma formation. The susceptible biotype also shows formation of aerenchyma when treated with quinclorac, but in lower levels than those observed for the resistant biotype

Indoor air quality in the sleeping environments of Lisbon dwellings, Portugal: preliminary results of HypnosAIR project

Trabalho apresentado em World Sleep Conference 2023, 20-25 outubro, Rio de janeiro, BrasilN/

Nonparametric estimation of conditional transition probabilities in a non-Markov illness-death model

One important goal in multi-state modeling is the estimation of transition probabilities. In longitudinal medical studies these quantities are particularly of interest since they allow for long-term predictions of the process. In recent years signi ficant contributions have been made regarding this topic. However, most of the approaches assume independent censoring and do not account for the influence of covariates. The goal of the paper is to introduce feasible estimation methods for the transition probabilities in an illness-death model conditionally on current or past covariate measures. All approaches are evaluated through a simulation study, leading to a comparison of two di erent estimators. The proposed methods are illustrated using real a colon cancer data set.This research was nanced by FEDER Funds through Programa Operacional Factores de Competitividade COMPETE and by Portuguese Funds through FCT - Funda ção para a Cência e a Tecnologia, within Projects Est-C/MAT/UI0013/2011 and PTDC/MAT/104879/2008. We also acknowledge nancial support from the project Grants MTM2008-03129 and MTM2011-23204 (FEDER support included) of the Spanish Ministerio de Ciencia e Innovaci on and 10PXIB300068PR of the Xunta de Galicia. Partial support from a grant from the US National Security Agency (H98230-11-1-0168) is greatly appreciated

Parametric hazard rate models for long-term sickness absence

PURPOSE: In research on the time to onset of sickness absence and the duration of sickness absence episodes, Cox proportional hazard models are in common use. However, parametric models are to be preferred when time in itself is considered as independent variable. This study compares parametric hazard rate models for the onset of long-term sickness absence and return to work. METHOD: Prospective cohort study on sickness absence with four follow-up years of 53,830 employees working in the private sector in the Netherlands. The time to onset of long-term (>6 weeks) sickness absence and return to work were modelled by parametric hazard rate models. RESULTS: The exponential parametric model with a constant hazard rate most accurately described the time to onset of long-term sickness absence. Gompertz-Makeham models with monotonically declining hazard rates best described return to work. CONCLUSIONS: Parametric models offer more possibilities than commonly used models for time-dependent processes as sickness absence and return to work. However, the advantages of parametric models above Cox models apply mainly for return to work and less for onset of long-term sickness absence

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia

Juvenile myelomonocytic leukemia (JMML) is a poor-prognosis childhood leukemia usually caused by RAS-pathway mutations. The cellular hierarchy in JMML is poorly characterized, including the identity of leukemia stem cells (LSCs). FACS and single-cell RNA sequencing reveal marked heterogeneity of JMML hematopoietic stem/progenitor cells (HSPCs), including an aberrant Lin-CD34+CD38-CD90+CD45RA+ population. Single-cell HSPC index-sorting and clonogenic assays show that (1) all somatic mutations can be backtracked to the phenotypic HSC compartment, with RAS-pathway mutations as a "first hit,"(2) mutations are acquired with both linear and branching patterns of clonal evolution, and (3) mutant HSPCs are present after allogeneic HSC transplant before molecular/clinical evidence of relapse. Stem cell assays reveal interpatient heterogeneity of JMML LSCs, which are present in, but not confined to, the phenotypic HSC compartment. RNA sequencing of JMML LSC reveals upregulation of stem cell and fetal genes (HLF, MEIS1, CNN3, VNN2, and HMGA2) and candidate therapeutic targets/biomarkers (MTOR, SLC2A1, and CD96), paving the way for LSC-directed disease monitoring and therapy in this disease

Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia

Recent advances in single-cell transcriptomics are ideally placed to unravel intratumoral heterogeneity and selective resistance of cancer stem cell (SC) subpopulations to molecularly targeted cancer therapies. However, current single-cell RNA-sequencing approaches lack the sensitivity required to reliably detect somatic mutations. We developed a method that combines high-sensitivity mutation detection with whole-transcriptome analysis of the same single cell. We applied this technique to analyze more than 2,000 SCs from patients with chronic myeloid leukemia (CML) throughout the disease course, revealing heterogeneity of CML-SCs, including the identification of a subgroup of CML-SCs with a distinct molecular signature that selectively persisted during prolonged therapy. Analysis of nonleukemic SCs from patients with CML also provided new insights into cell-extrinsic disruption of hematopoiesis in CML associated with clinical outcome. Furthermore, we used this single-cell approach to identify a blast-crisis-specific SC population, which was also present in a subclone of CML-SCs during the chronic phase in a patient who subsequently developed blast crisis. This approach, which might be broadly applied to any malignancy, illustrates how single-cell analysis can identify subpopulations of therapy-resistant SCs that are not apparent through cell-population analysis

Correlações entre taxa de crescimento da ferrugem do cafeeiro e variáveis meteorológicas.

O presente estudo teve por objetivo determinar quais variáveis meteorológicas exercem maior influência no desenvolvimento da ferrugem do cafeeiro, bem como qual o período de maior importância de cada uma delas para a doença.CBAgro 2015

Particle tracer transport in a sloping soil lysimeter under periodic, steady state conditions

Colloid transport through complex and dynamic (i.e. non-steady-state) hydrologic systems is rarely studied, owing to the difficulty of constraining initial and boundary conditions and quantifying colloid-porous media and colloid-colloid interactions in transient flow systems. Here we present a particle tracer experiment conducted on a sloped lysimeter receiving periodic rainfall events for 10 days. Four unique, DNA-labelled particle tracers were injected both in sequence and in parallel, together with a conservative tracer (deuterium), over the course of the first day and allowed to move through the system. Discharge-particle tracer concentration curves and the spatial distribution of particle tracer mass retained in the soil at the end of the experiment were found to be highly dependent on the timing of the tracer injection and the precipitation input and subsequent dynamic response of the water table. Overall, neglecting the total DLT recovery rate, the DLT particle tracer breakthrough trend (DNA-labelled particle tracer 4) was similar to deuterium and decreased over time with the exception of a few peaks later in the experiment. The individual particle tracer breakthrough curves suggest a complex system with different fast transport mechanisms (e.g. capillary barrier and size exclusion effect) and slow retention-release mechanisms (e.g. straining, physical-chemical adsorption), which resulted in particle tracers transferring faster than deuterium in the first 10 h of the experiment but being exceeded by deuterium soon after deuterium started to break through. The experiment not only highlights the interaction of repeated colloidal pollution events in hydrologic systems with different pre-event saturation conditions, but also the benefits of using multiple synchronous or sequential tracer applications to dissect explicit formulations of water flow and colloid transport processes in complex and dynamic hydrological systems. Such explicit process formulations could help improve understanding hydrologically-controlled transport through catchments and the quantitative prediction of these processes with water quality models