EFFECT OF Salsola vermiculata

Algerian aride zone is rich with medicinal plant, Salsola vermiculata is one of the mosttraditional plant remedies in Bechar region. in this work, we tried to test the inhibitory effect on eight storage soft wheat fungi of various extracts of Salsola vermiculata in vitro and vivo by using grain storage by the coating method. The phytochemical screening of plants showed the detection of tannins, saponisides and flavonoids. The results show a remarkable antifungal effect by most of the extracts tested. The  decrease in the level of fungi observed in wheat samples demonstrates the effectiveness of grain storage by the coating method.Keywords: Salsola vermiculata; Antifungal activity; Coating method; Fungi; soft wheat;Storage

Etude éco-biologique d’Artémia salina des zones humides de l’Ouest Algérien

L’Artémie (Artémia salina) est une espèce de crustacé vivant dans les lacs salés, les chotts et les marais salants .Elle est en abondance dans les salines de l’ouest Algérien. Ce genre de crustacé est très demandé dans le domaine d’exploitation des ressources hydriques et en aquaculture. Dans le présent travail on a étudié, en premier lieu l’aspect biologique et écologique de ce crustacé dans les salines de Bathioua (wilaya d’Oran) et Oued Djemaa (wilaya de Relizane), en deuxième lieu on a étudié son accommodation à différentes doses de salinité dans un milieu même quand les conditions de vie sont défavorables. L’Artémie salina est capable de produire des cystes qui ont la capacité de donner naissance à une larve appelée nauplius. La résistivité des cystes au stress du milieu en état de cryptobiose, leur permet de subsister à des températures extrêmes. Les nauplius sont distribués dès l’éclosion ou après 24 à 48 h d’enrichissement en protéines et en lipides. Les résultats obtenus lors de ce travail ont montré que le taux de reproduction varie en fonction des changements saisonniers des différents facteurs écologiques et des éléments physico chimiques de son environnement.Mots-clés: Artémia salina, aspect biologique, adaptation, salines, Ouest Algérien. Eco-biological study of Artemia salina wetlands of West AlgeriaThe Artemia (Artemia salina) is a species of crustacean living in salt lakes, chotts and salt marshes. It is in abundance in the salt mines of western Algeria. This kind of crustacean is highly demanded in the fields of water exploitation and aquaculture. In this study, the observation is drawn on the biological and ecological aspects of this crustacean in the salt mines of Bathioua (Oran wilaya) and Oued Djemaa (Relizane wilaya), according to the seasonal variations. Artemia salina is able to produce cysts that have the ability to give birth to a larva called nauplius. The resistivity of cysts at environment stress on cryptobiosis state allows them to survive in extreme temperatures. Nauplii begin hatching after 24 to 48 h enrichment in proteins and lipids. The results obtained in this work showed that the reproduction rate varies according to seasonal changes in different ecological factors and the physical and chemical elements of its environment.Keywords : Artemia salina, biological aspect, adaptation, salt, West Algeria

The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content

The antidiabetic effect of N. sativa seed ethanol extract (NSE) was assessed in Meriones shawi after development of diabetes. Meriones shawi were divided randomly into four groups: normal control, diabetic control, diabetic treated with NSE (2 g eq plant/kg) or with metformin (300 mg/kg) positive control, both administered by daily intragastric gavage for 4 weeks. Glycaemia and body weight were evaluated weekly. At study's end, an Oral Glucose Tolerance Test (OGTT) was performed to estimate insulin sensitivity. Upon sacrifice, plasma lipid profile, insulin, leptin, and adiponectin levels were assessed. ACC phosphorylation and Glut4 protein content were determined in liver and skeletal muscle. NSE animals showed a progressive normalization of glycaemia, albeit slower than that of metformin controls. Moreover, NSE increased insulinemia and HDL-cholesterol, compared to diabetic controls. Leptin and adiponectin were unchanged. NSE treatment decreased OGTT and tended to decrease liver and muscle triglyceride content. NSE stimulated muscle and liver ACC phosphorylation and increased muscle Glut4. These results confirm NSE's previously reported hypoglycaemic and hypolipidemic activity. More significantly, our data demonstrate that in vivo treatment with NSE exerts an insulin-sensitizing action by enhancing ACC phosphorylation, a major component of the insulin-independent AMPK signaling pathway, and by enhancing muscle Glut4 expression

Chemical composition and anti-arthritic activity of Anacyclus valentinus extract on adjuvant-induced arthritis in rats

Anacyclus valentinus L. is a common annual plant in Algeria, known for her various therapeutic effects. In addition, the plant is used as a food condiment. We reported our investigations on the chemical compositions and the antiarthritic activity of methanolic extract of A. valentinus (MEAV). The polyphenol extraction by maceration with methanol (80%) gave yields of 17.82%. The identification by LC-MS and colorimetric assays revealed the wealth of methanolic extracts on phenolic compounds including flavonoids (52.15 mg Equ/g) and lactones. Acute oral toxicity of extract was performed in line with OECD guidelines and the lethal dose 50 was assessed greater than 2500 mg/Kg. Regarding the anti-arthritic power, rheumatoid arthritis was induced by Freund\u27s adjuvant in rats. The methanolic extract of A. valentinus presented a largest effect with weight gain, an arthritic score, thymus indices; spleen and serum parameters close to those of the control. The extract also inhibited edema and restored cartilage structure

Influence de la teneur de nickel sur le comportement tribologique et électrochimique de l’alliage TiNi.

Parmi les matériaux métalliques, les alliages de TiNi sont employés principalement pour des applications biomédicales et/ou dentaires dues à leur meilleure compatibilité mécanique avec les tissus, leur module de Young proche de celui de l’os et une résistance élevée à la corrosion dans les fluides du corps et une bonne biocompatibilité. Pour cette raison, le comportement à la corrosion et à l’usure de l’alliage TiNi avec des teneurs en Ni varie de 40 à 60% en poids, ont été étudiés afin de vérifier l’effet du Ni sur la biocompatibilité de cet alliage pour des applications dentaires

Surface performances of Ti-6Al-4V substrates coated PVD multilayered films in biological environments

Multilayered and nanostructured coatings of Ti based alloys (oxides and nitrides) are elaborated and tested for increasing protective properties such as corrosion and wear resistances. A pin-on-disc tribometer was used to evaluate the wear resistance in Hank's solution against bovine bone. Corrosion behavior in Hank's solution was determined by potentiodynamic and electrochemical impedance spectroscopy techniques. Besides, the specimen surfaces were characterized by Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) microanalyses. The results showed that optimal tribological properties were obtained in the case of coatings having TiN as top layer. The main wear mechanism was abrasive third body wear. In vitro corrosion tests at 37 °C showed that the better corrosion resistance was obtained when TiN was the top layer. However all of them exhibited good tribological properties, good corrosion resistance and then may be promising options for biomedical applications

Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo – a new animal model

BACKGROUND: Existing animal models provide only indirect information about the pathogenesis of infections caused by indigenous gastrointestinal microflora and the kinetics of bacterial translocation. The aim of this study was to develop a novel animal model to assess bacterial translocation and intestinal barrier function in vivo. METHODS: In anaesthetized male Wistar rats, 0.5 ml of a suspension of green fluorescent protein-transfected E. coli was administered by intraluminal injection in a model of small bowel obstruction. Animals were randomly subjected to non-ischemic or ischemic bowel obstruction. Ischemia was induced by selective clamping of the terminal mesenteric vessels feeding the obstructed bowel loop. Time intervals necessary for translocation of E. coli into the submucosal stroma and the muscularis propria was assessed using intravital microscopy. RESULTS: Bacterial translocation into the submucosa and muscularis propria took a mean of 36 ± 8 min and 80 ± 10 min, respectively, in small bowel obstruction. Intestinal ischemia significantly accelerated bacterial translocation into the submucosa (11 ± 5 min, p < 0.0001) and muscularis (66 ± 7 min; p = 0.004). Green fluorescent protein-transfected E. coli were visible in frozen sections of small bowel, mesentery, liver and spleen taken two hours after E. coli administration. CONCLUSIONS: Intravital microscopy of fluorescent bacteria is a novel approach to study bacterial translocation in vivo. We have applied this technique to define minimal bacterial transit time as a functional parameter of intestinal barrier function