Application of extracts from the poisonous plant, Nerium Oleander L., as a wood preservative

The antifungal properties of poisonous plant extracts from oleanders (Nerium oleander L.) were determined when used as a wood preservative. The extract was prepared from oleanders leaves andflowers in 96% ethyl alcohol. The wood blocks of Turkish oriental beech (Fagus orientalis L.) and Scots pine (Pinus sylvestris L.) were impregnated with the extracts. The abilities of the extract to suppressattack by Postia placenta (Fr.) (brown rot) and Trametes versicolor (L: Fr.) Quel. (a white-rot) was investigated. Treated blocks were exposed to P. placenta and T. versicolor attacks for 12 weeks by following the soil-block method. While untreated wood specimens have weight loss ranging between 27.37 and 30.66% for P. placenta and 8.64 and 24.06% for T. versicolor, the wood treated with theextracts is of weight loss between 5.54 and 10.98% for P. placenta, and between 5.02 and 28.25% for T. versicolor. The lowest weight loss was found to be for beech wood (5.02%) impregnated with the extractof oleander at a concentration level of 0.25% against T. versicolor. While the highest weight loss was also on the beech wood (28.25%) treated with the same extract at the level of 0.50% concentrationagainst T. versicolor. In conclusion, the extracts could be used as effective wood preservative

The inhibition of FGF receptor 1 activity mediates sorafenib-induced antiproliferative effects in human mesothelioma tumor-initiating cells

Tumor-initiating cells (TICs), the subset of cells within tumors endowed with stem-like features, being highly resistant to conventional cytotoxic drugs, are the major cause of tumor relapse. The identification of molecules able to target TICs remains a significant challenge in cancer therapy. Using TIC-enriched cultures (MM1, MM3 and MM4), from 3 human malignant pleural mesotheliomas (MPM), we tested the effects of sorafenib on cell survival and the intracellular mechanisms involved. Sorafenib inhibited cell-cycle progression in all the TIC cultures, but only in MM3 and MM4 cells this effect was associated with induction of apoptosis via the down-regulation of Mcl-1. Although sorafenib inhibits the activity of several tyrosine kinases, its effects are mainly ascribed to Raf inhibition. To investigate the mechanisms of sorafenib-mediated antiproliferative activity, TICs were treated with EGF or bFGF causing, in MM3 and MM4 cells, MEK, ERK1/2, Akt and STAT3 phosphorylation. These effects were significantly reduced by sorafenib in bFGF-treated cells, while a slight inhibition occurred after EGF stimulation, suggesting that sorafenib effects are mainly due to FGFR inhibition. Indeed, FGFR1 phosphorylation was inhibited by sorafenib. A different picture was observed in MM1 cells, which, releasing high levels of bFGF, showed an autocrine activation of FGFR1 and a constitutive phosphorylation/activation of MEK-ERK1/2. A powerful inhibitory response to sorafenib was observed in these cells, indirectly confirming the central role of sorafenib as FGFR inhibitor. These results suggest that bFGF signaling may impact antiproliferative response to sorafenib of MPM TICs, which is mainly mediated by a direct FGFR targeting

p16INK4A Positively Regulates Cyclin D1 and E2F1 through Negative Control of AUF1

/pRB/E2F pathway, a key regulator of the critical G1 to S phase transition of the cell cycle, is universally disrupted in human cancer. However, the precise function of the different members of this pathway and their functional interplay are still not well defined. -dependent manner, and several of these genes are also members of the AUF1 and E2F1 regulons. We also present evidence that E2F1 mediates p16-dependent regulation of several pro- and anti-apoptotic proteins, and the consequent induction of spontaneous as well as doxorubicin-induced apoptosis. is also a modulator of transcription and apoptosis through controlling the expression of two major transcription regulators, AUF1 and E2F1

Systematic Review of Potential Health Risks Posed by Pharmaceutical, Occupational and Consumer Exposures to Metallic and Nanoscale Aluminum, Aluminum Oxides, Aluminum Hydroxide and Its Soluble Salts

Aluminum (Al) is a ubiquitous substance encountered both naturally (as the third most abundant element) and intentionally (used in water, foods, pharmaceuticals, and vaccines); it is also present in ambient and occupational airborne particulates. Existing data underscore the importance of Al physical and chemical forms in relation to its uptake, accumulation, and systemic bioavailability. The present review represents a systematic examination of the peer-reviewed literature on the adverse health effects of Al materials published since a previous critical evaluation compiled by Krewski et al. (2007). Challenges encountered in carrying out the present review reflected the experimental use of different physical and chemical Al forms, different routes of administration, and different target organs in relation to the magnitude, frequency, and duration of exposure. Wide variations in diet can result in Al intakes that are often higher than the World Health Organization provisional tolerable weekly intake (PTWI), which is based on studies with Al citrate. Comparing daily dietary Al exposures on the basis of “total Al”assumes that gastrointestinal bioavailability for all dietary Al forms is equivalent to that for Al citrate, an approach that requires validation. Current occupational exposure limits (OELs) for identical Al substances vary as much as 15-fold. The toxicity of different Al forms depends in large measure on their physical behavior and relative solubility in water. The toxicity of soluble Al forms depends upon the delivered dose of Al+ 3 to target tissues. Trivalent Al reacts with water to produce bidentate superoxide coordination spheres [Al(O2)(H2O4)+ 2 and Al(H2O)6 + 3] that after complexation with O2•−, generate Al superoxides [Al(O2•)](H2O5)]+ 2. Semireduced AlO2• radicals deplete mitochondrial Fe and promote generation of H2O2, O2 • − and OH•. Thus, it is the Al+ 3-induced formation of oxygen radicals that accounts for the oxidative damage that leads to intrinsic apoptosis. In contrast, the toxicity of the insoluble Al oxides depends primarily on their behavior as particulates. Aluminum has been held responsible for human morbidity and mortality, but there is no consistent and convincing evidence to associate the Al found in food and drinking water at the doses and chemical forms presently consumed by people living in North America and Western Europe with increased risk for Alzheimer\u27s disease (AD). Neither is there clear evidence to show use of Al-containing underarm antiperspirants or cosmetics increases the risk of AD or breast cancer. Metallic Al, its oxides, and common Al salts have not been shown to be either genotoxic or carcinogenic. Aluminum exposures during neonatal and pediatric parenteral nutrition (PN) can impair bone mineralization and delay neurological development. Adverse effects to vaccines with Al adjuvants have occurred; however, recent controlled trials found that the immunologic response to certain vaccines with Al adjuvants was no greater, and in some cases less than, that after identical vaccination without Al adjuvants. The scientific literature on the adverse health effects of Al is extensive. Health risk assessments for Al must take into account individual co-factors (e.g., age, renal function, diet, gastric pH). Conclusions from the current review point to the need for refinement of the PTWI, reduction of Al contamination in PN solutions, justification for routine addition of Al to vaccines, and harmonization of OELs for Al substances

Wild species of vaccinium composition, nutritional value and utilization

There are still, in many parts of the globe, some edible fruit collects from the wild for human feeding and other uses. These fruits are utilised either in their raw nature or after some form of processing. One of these wild fruits are Vaccinium species such as Vaccinium myrtoides (Blume) Miq., Vaccinium cylindraceum, Vaccinium padifolium, Vaccinium corymbosum, Vaccinium myrtillus and others from the plant family Ericaceae. The term wild infers non-cultivated plants found in plantation felids or the forest. The species Vaccinium myrtoides and the others are small trees (shrubs) that are well known in Southeast Asian countries such as Philippines, Indonesia and other neighbouring islands. The plants classified as wild growing shrubs. However, the locals have made it be fully utilized in some areas as the wood that are used in grafting utensils and cutleries, or as fuel and fruits. The small-sized berry-like fruits with an average diameter of about 4–5 mm turn black when ripe. They have a delicious flavour that makes it edible and used in the making of other delicacies such as tart and pies to add flavour and as preservatives as well. A few published researches were done on Vaccinium myrtoides showing that it is still used in folkloric medicine. Leaves and fruits extracts showed strong antioxidant activity when tested in-vitro using DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging activity as part of a preliminary phytochemical screening for V. myrtoides. The study revealed that the antioxidant activity is due to the presence of flavonoids and other phenolic compounds in the plant leaves and fruit extracts

MAGIC and Fermi-LAT gamma-ray results on unassociated HAWC sources

The HAWC Collaboration released the 2HWC catalogue of TeV sources, in which 19 show no association with any known high-energy (HE; E greater than or similar to 10 GeV) or very-high-energy (VHE; E greater than or similar to 300 GeV) sources. This catalogue motivated follow-up studies by both the Major Atmospheric Gamma-ray Imaging Cherenkov (MAGIC) and Fermi-LAT (Large Area Telescope) observatories with the aim of investigating gamma-ray emission over a broad energy band. In this paper, we report the results from the first joint work between High Altitude Water Cherenkov (HAWC), MAGIC, and Fermi-LAT on three unassociated HAWC sources: 2HWC J2006+341, 2HWC J1907+084*, and 2HWC J1852+013*. Although no significant detection was found in the HE and VHE regimes, this investigation shows that a minimum 1 degrees extension (at 95 per cent confidence level) and harder spectrum in the GeV than the one extrapolated from HAWC results are required in the case of 2HWC J1852+013*, whilst a simply minimum extension of 0.16 degrees (at 95 per cent confidence level) can already explain the scenario proposed by HAWC for the remaining sources. Moreover, the hypothesis that these sources are pulsar wind nebulae is also investigated in detail

The estimation of phenotypic and genetic parameters for calf birth weight and gestation length of Brown Swiss cows reared at the Bahri Dagdas International Agricultural Research Institute

This study was carried out to estimate the phenotypic and genetic parameters for the gestation length and birth weight of Brown Swiss cattle reared at the Bahri Bagda International Agricultural Research Institute. The experimental material consisted of 891 calving records and 666 gestation length records between 1989 and 2000. The least-squares means of birth weight and gestation length were 35.6 kg and 284 days, respectively. Except for the effect of the dam's age on gestation length, all other factors investigated in this study had significant effects on both gestation length and birth weight (P < 0.05-0.001). The estimations of heritability and repeatability for birth weight were 0.10 and 0.15, respectively. Estimated repeatability for gestation length was 0.14

a wood preservative

The antifungal properties of poisonous plant extracts from oleanders ( Nerium oleander L.) were determined when used as a wood preservative. The extract was prepared from oleanders leaves and flowers in 96% ethyl alcohol. The wood blocks of Turkish oriental beech (Fagus orientalis L.) and Scots pine ( Pinus sylvestris L.) were impregnated with the extracts. The abilities of the extract to suppress attack by Postia placenta (Fr.)(brown rot) and Trametes versicolor (L: Fr.) Quel. (a white-rot) was investigated. Treated blocks were exposed to P. placenta and T. versicolor attacks for 12 weeks by following the soil-block method. While untreated wood specimens have weight loss ranging between 27.37 and 30.66% for P. placenta and 8.64 and 24.06% for T. versicolor, the wood treated with the extracts is of weight loss between 5.54 and 10.98% for P. placenta, and between 5.02 and 28.25% for T. versicolor. The lowest weight loss was found to be for beech wood (5.02%) impregnated with the extract of oleander at a concentration level of 0.25% against T. versicolor. While the highest weight loss was also on the beech wood (28.25%) treated with the same extract at the level of 0.50% concentration against T. versicolor. In conclusion, the extracts could be used as effective wood preservative.C1 Mugla Univ, Fac Technol, Dept Wood Sci & Furniture Design, TR-48000 Mugla, Turkey.Pamukkale Univ, Fac Arts & Sci, Dept Biol, Denizli, Turkey.Mugla Univ, Fac Arts & Sci, Dept Chem, TR-48000 Mugla, Turkey.Adnan Menderes Univ, Fac Agr, Dept Hort, TR-09100 Aydin, Turkey