Genetic diversity in Brazilian populations of Aedes albopictus

Random amplified polymorphic DNA (RAPD) analysis technique was undertaken in Aedes albopictus populations from three states in Brazil, Rio de Janeiro (RJ), Minas Gerais (MG) and Pernambuco (PE), to estimate the level of genetic variability and levels of genetic exchange between populations. Allele and genotype frequencies were measured on 47 RAPD loci. Average observed heterozigosity (Ho) ranged from 0.282 in MG to 0.355 in Casa Forte (PE) population. Genetic distances estimates indicated that RJ and MG were more genetically similar than populations from PE. Genetic variation observed in local Brazilian populations was attributed to genetic drift associated with restricted gene flow in recently established populations

Evaluation of a new formulation of Bacillus thuringiensis israelensis

The aim of this study was to determine the potency (ITU) and efficacy of a liquid formulation of Bacillus thuringiensis israelensis developed by the State University of Londrina named BioUel, against early fourth instar larvae of Aedes aegypti and Culex quinquefasciatus. The ITU/mg of BioUel was 960, the LC50 was of 0.271 (± 0.39) ppm, and the LC95 was 0.634 (± 0.099) ppm, in larvae of C. quinquefasciatus. In A. aegypti larvae, LC50 was 0.332 (± 0.042) ppm and LC95 was 0.694 (± 0.073) ppm. The ITU level of BioUel and its control results were similar to most commercial products tested. Stability was of approximately 90 days, which allows for local production

Integralidade e cuidado a grávidas adolescentes na Atenção Básica Comprehensive care of pregnant adolescents in Primary Care

O pré-natal em adolescentes tem se caracterizado por ações técnicas buscando-se na atualidade qualificar o cuidado na perspectiva da integralidade. O Programa de Agentes Comunitários de Saúde/Programa Saúde da Família (PACS/PSF) constitui estratégia para avançar nas mudanças requeridas, o que motivou esta investigação científica, que teve como objetivos conhecer o processo de cuidado pré-natal a adolescentes grávidas por profissionais de saúde do PACS/PSF e analisá-lo na perspectiva da integralidade. É um estudo qualitativo, tendo a integralidade como enfoque teórico. Foi desenvolvido em unidades de PACS/PSF em um município da Bahia, sendo sujeitos do estudo profissionais que cuidam de gestantes adolescentes. O material empírico foi produzido por meio de entrevista semiestruturada e observação não participante, em consultas e visitas domiciliárias e analisado pela técnica de análise de discurso. A análise revelou que o cuidado pré-natal à adolescente se concretiza sob o modelo biomédico, e o cumprimento de rotinas e protocolos institucionais sobrepõe-se à singularidade da adolescente. Assim, distancia-se da integralidade, na medida em que as práticas são orientadas por sujeições ideológicas a um modelo que nega às adolescentes grávidas a oportunidade de serem sujeitos.<br>Prenatal adolescent care is characterized by technical actions seeking to provide assistance from the perspective of comprehensive care. The Community Health Agent Program/Family Health Program (PACS/PSF) is a strategy for implementing the changes envisaged, which motivated this scientific investigation. The scope of the research was to assess prenatal care to pregnant adolescents by health professionals of PACS/PSF and analyze it from the standpoint of comprehensive care. It is a qualitative study, with comprehensive care as its theoretical focus. It was conducted in PACS/PSF units in a city in the State of Bahia, and the subjects of study were the practitioners who treat pregnant adolescents. The empirical material was produced by means of semi-structured interviews and non-participative observation in consultations and homecare visits and was analyzed using the speech analysis technique. This revealed that prenatal care for adolescents is provided using the biomedical model, where compliance with institutional protocols and routines takes precedence over considering the specific aspects of the adolescent. Thus it is far removed from comprehensive care, in that the practices are guided by ideological adherence to a model that fails to grant pregnant adolescents the opportunity to be treated as individuals

Decreased Toxicity of Bacillus thuringiensis subsp. israelensis to Mosquito Larvae after Contact with Leaf Litter

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