Seleção genética precoce em mudas de imbuia com vistas à conservação de germoplasma.

EVINCI. Resumo 002

Produção de mudas de guanandi.

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Dinâmica da germinação de sementes de populações de imbuia (Ocotea porosa Nees et Martius ex. Nees, Lauraceae).

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Produção de mudas de imbuia.

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Estudo sensorial do palmito em conserva com o 'in natura" de pupunha (Bactris gasipaes Kunth.) obtido por processo artesanal.

Resumo

Associação entre diâmetro e peso com emergência de sementes de imbuia (Ocotea porosa) em viveiro.

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Pré-melhoramento de populações de imbuia.

A imbuia (Ocotea porosa) é uma espécie em extinção da Floresta Ombrófila Mista, cuja madeira possui alto valor econômico. O objetivo deste trabalho foi efetuar a seleção genética precoce individual por meio do programa Selegen REML/BLUP em germoplasma oriundo das populações de Colombo, PR, Canoinhas e Caçador, SC. Os resultados mostraram que, de um total de 4.888 genótipos, 1.182 genótipos selecionados (42,9 %) eram de Colombo, 1.004 (36,4 %) de Canoinhas e 572 (20,7 %) de Caçador, num total de 2.758 genótipos selecionados. As estimativas dos componentes de variância obtidas para altura de mudas mostraram que a variância genética aditiva dentro de procedências (Va=6,62) é maior que a variância entre procedências (Vproc.=1,33), indicando a possibilidade de predominância de alogamia nas populações estudadas. A herdabilidade individual no sentido restrito no bloco (h2 a), ou seja, dos efeitos aditivos, foi de 0,29 ± 04. O coeficiente de variação genético individual (Cvgi %) foi de 18,72 %. A classificação dos 50 melhores genótipos mostrou ganho de 5,66 cm a 8,72 cm, com estimativa de médias da geração seguinte variando de 19,04 cm a 22,45 cm (de 41,19 % a 63,46 % de superioridade). A procedência Colombo contribuiu com 56 % dos 50 melhores genótipos, Canoinhas com 40 % e Caçador com apenas 4 %.Nota Científica

Pré-melhoramento de imbuia (Ocotea porosa) visando à sua conservação ex-situ.

Resumo

Prospective assessment of integrating the existing emergency medical system with automated external defibrillators fully operated by volunteers and laypersons for out-of-hospital cardiac arrest: the Brescia Early Defibrillation Study (BEDS)

AIMS: There are few data on the outcomes of cardiac arrest (CA) victims when the defibrillation capability of broad rural and urban territories is fully operated by volunteers and laypersons. METHODS AND RESULTS: In this study, we investigated whether a programme based on diffuse deployment of automated external defibrillators (AEDs) operated by 2186 trained volunteers and laypersons across the County of Brescia, Italy (area: 4826 km(2); population: 1 112 628), would safely and effectively impact the current survival among victims of out-of-hospital CA. Forty-nine AEDs were added to the former emergency medical system that uses manual EDs in the emergency department of 10 county hospitals and in five medically equipped ambulances. The primary endpoint was survival free of neurological impairment at 1-year follow-up. Data were analysed in 692 victims before and in 702 victims after the deployment of the AEDs. Survival increased from 0.9% (95% CI 0.4-1.8%) in the historical cohort to 3.0% (95% CI 1.7-4.3%) (P=0.0015), despite similar intervals from dispatch to arrival at the site of collapse [median (quartile range): 7 (4) min vs. 6 (6) min]. Increase of survival was noted both in the urban [from 1.4% (95% CI 0.4-3.4 %) to 4.0% (95% CI 2.0-6.9 %), P=0.024] and in the rural territory [from 0.5% (95% CI 0.1-1.6%) to 2.5% (95% CI 1.3-4.2%), P=0.013]. The additional costs per quality-adjusted life year saved amounted to euro39 388 (95% CI euro16 731-49 329) during the start-up phase of the study and to euro23 661 (95% CI euro10 327-35 528) at steady state. CONCLUSION: Diffuse implementation of AEDs fully operated by trained volunteers and laypersons within a broad and unselected environment proved safe and was associated with a significantly higher long-term survival of CA victims

Urogenital Abnormalities in Adenosine Deaminase Deficiency

BACKGROUND: Improved survival in ADA-SCID patients is revealing new aspects of the systemic disorder. Although increasing numbers of reports describe the systemic manifestations of adenosine deaminase deficiency, currently there are no studies in the literature evaluating genital development and pubertal progress in these patients. METHODS: We collected retrospective data on urogenital system and pubertal development of 86 ADA-SCID patients followed in the period 2000-2017 at the Great Ormond Street Hospital (UK) and 5 centers in Italy. In particular, we recorded clinical history and visits, and routine blood tests and ultrasound scans were performed as part of patients' follow-up. RESULTS AND DISCUSSION: We found a higher frequency of congenital and acquired undescended testes compared with healthy children (congenital, 22% in our sample, 0.5-4% described in healthy children; acquired, 16% in our sample, 1-3% in healthy children), mostly requiring orchidopexy. No urogenital abnormalities were noted in females. Spontaneous pubertal development occurred in the majority of female and male patients with a few cases of precocious or delayed puberty; no patient presented high FSH values. Neither ADA-SCID nor treatment performed (PEG-ADA, BMT, or GT) affected pubertal development or gonadic function. CONCLUSION: In summary, this report describes a high prevalence of cryptorchidism in a cohort of male ADA-SCID patients which could represent an additional systemic manifestation of ADA-SCID. Considering the impact urogenital and pubertal abnormalities can have on patients' quality of life, we feel it is essential to include urogenital evaluation in ADA-SCID patients to detect any abnormalities, initiate early treatment, and prevent long-term complications