4 research outputs found
Cassava processing wastewater as a platform for third generation biodiesel production
ABSTRACT This study aimed to evaluate third generation biodiesel production by microalgae Phormidium autumnale using cassava processing wastewater as a platform. Experiments were performed in a heterotrophic bubble column bioreactor. The study focused on the evaluation of the bioreactor (batch and fed-batch) of different operational modes and the analysis of biofuel quality. Results indicate that fed-batch cultivations improved system performance, elevating biomass and oil productions to 12.0 g L−1 and 1.19 g L−1, respectively. The composition of this oil is predominantly saturated (60 %) and monounsaturated (39 %), resulting in a biodiesel that complys with U.S., European and Brazilian standards. The technological route developed indicates potential for sustainable production of bulk oil and biodiesel, through the minimization of water and chemical demands required to support such a process
Third Generation Biodiesel Production From Microalgae [produção De Biodiesel De Terceira Geração A Partir De Microalgas]
The aim of the study was to evaluate the third generation biodiesel produced from heterotrophic cultivation of the microalgae Phormidium sp. employing cassava starch as source of organic carbon. An experimental design was performed to determine the optimal conditions of temperature and C/N ratio. From the best growing conditions, was developed cultivations in batch and fed-batch in a bioreactor and evaluated the biodiesel quality. The results indicate that the temperature of 30ºC and the C/N ratio of 68 are the ideal conditions of the process. The highest biomass productivity (50.41mgL−1h-1) and lipid productivity (7.49mgL−1h-1) were obtained in batch cultivations. The fatty acids most representative were caproic acid (65.29%) and oleic acid (23.88%). The fuel properties of biodiesel: ester content of 99.9%, cetane number of 54.88, iodine value of 21.47gI100g−1, unsaturation degree of 23.88% and a cold filter plugging point of 39.21ºC, comply with the main international and national standards.452349355(2003) Provisional Brazilian Biodiesel Standard ANP, 21p. , 255, Brasil, Total de(2005) Standard Methods for the Examination of Water and Wastewater, 1325p. , 21ed. Baltimore, Maryland: Prot CityBlend Stock for Distillate Fuels. United States of America (2002) The biodiesel handbook, 332p. , In: KNOTHE, G. et al. (Ed.). Champaign, Illinois: AOCS, 2005Bligh, E.G., Dyer, J.W., A rapid method of total lipid extraction and purification (1959) Canadian Journal of Biochemistry and Physiology, 37 (8), pp. 911-917. , http://www.nrcresearchpress.com/doi/abs/10.1139/o59-099#.U_OV2fldXaM, DisponÃvel em, Acesso em: 28 jan. 2010Francisco, E.C., Microalgae as feedstock for biodiesel production: Carbon dioxide sequestration, lipid production and biofuel quality (2010) Journal of Chemistry Technology and Biotechnology, 85, pp. 395-403. , http://onlinelibrary.wiley.com/doi/10.1002/jctb.2338/abstract, DisponÃvel em, Acesso em: 29 jan. 2010Haddad, M., Fawaz, Z., Evaluation of microalgae alternative jet fuel using the AHP (2013) Method with a Emphasis on the Environmental Progress & Sustainable Energy, 32 (3), pp. 3044-3064. , http://onlinelibrary.wiley.com/doi/10.1002/ep.11638/abstract, DisponÃvel em, Acesso em: 06 fev. 2013Hartman, L., Lago, R.C.A., A rapid preparation of fatty acid methyl esters from lipids (1976) Laboratory Pratice, 22, pp. 475-476Krisnangkura, K., A Simple method for estimation of cetane index of vegetable oil methyl esters (1986) JAOCS, 63 (4), pp. 552-553. , http://link.springer.com/article/10.1007%2FBF02645752, DisponÃvel em, Acesso em: 29 jan. 2010Li, X., Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors (2007) Biotechnology and Bioengineering, 98 (4), pp. 764-771. , http://www.ncbi.nlm.nih.gov/pubmed/17497732, DisponÃvel em, Acesso em: 29 jan. 2010Lu, Y., Simultaneous saccharification of cassava starch and fermentation of algae for biodiesel production (2011) Journal Applied Phycology, 23, pp. 115-121. , http://link.springer.com/article/10.1007%2Fs10811-010-9549-z, DisponÃvel em, Acesso em: 06 fev. 2013MartÃn, M., Grossmann, I.E., On the synthesis of sustainable biorefineries (2012) Industrial & Engineering Chemistry Research, 52, pp. 3044-3064. , http://pubs.acs.org/doi/abs/10.1021/ie2030213, DisponÃvel em, Acesso em: 06 fev. 2013Mata, T.M., Microalgae for biodiesel production and their applications: A review (2010) Renewable & Sustainable Energy Reviews, 14, pp. 217-232. , http://www.sciencedirect.com/science/article/pii/S1364032109001646, DisponÃvel em, Acesso em: 29 jan. 2010Mohamed, M.S., Heterotrophic cultivation of microalgae for production of biodiesel (2011) Recent Patents on Biotechnology, 5, pp. 95-107. , http://www.ncbi.nlm.nih.gov/pubmed/21707527, DisponÃvel em, Acesso em: 06 fev. 2013Perez-Garcia, O., Heterotrophic cultures of microalgae: Metabolism and potential products (2011) Water Research, 45, pp. 11-36. , http://www.sciencedirect.com/science/article/pii/S0043135410006019, DisponÃvel em, Acesso em: 06 fev. 2013Queiroz, M.I., Fish processing wastewater as a platform of the microalgal biorefineries (2013) Biosystems Engineering, 115, pp. 195-202. , http://www.sciencedirect.com/science/article/pii/S1537511012002243, DisponÃvel em, Acesso em: 06 fev. 2013Ramos, M.J., Influence of fatty acid composition of raw materials in biodiesel properties (2009) Bioresource Technology, 100, pp. 261-268. , http://www.sciencedirect.com/science/article/pii/S0960852408005464, DisponÃvel em, Acesso em: 29 jan. 2010Rippka, R., Generic assignments strain histories and properties of pure cultures of cyanobacteria (1979) Journal of General Microbiology, 111 (1), p. 61. , http://mic.sgmjournals.org/content/111/1/1.short, DisponÃvel em, Acesso em: 29 jan. 2010(2004) STATISTICA for Windows [Computer program manual], , http://www.statsoftinc.com, [Online]. Statsoft Inc., Tulsa, Oklahoma, DisponÃvel em, Acesso em: 29 jan. 2010Suali, E., Sarbatly, R., Conversion of microalgae to biofuel (2012) Renewable and Sustainable Energy Reviews, 16, pp. 4316-4342. , http://www.sciencedirect.com/science/article/pii/S1364032112002304, DisponÃvel em, Acesso em: 06 fev. 2013Automotive Fuels, Fatty Acid Methyl Esters (FAME) for Diesel Engines, Requirements and TestMethods (2005) The biodiesel handbook Champaign, 332p. , UNE-EN 14214 European Union, 2003. 15p. In: KNOTHE, G.et al. (Ed.). Illinois: AOCSXu, H., High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters (2006) Journal of Biotechnology, 126, pp. 499-507. , http://www.ncbi.nlm.nih.gov/pubmed/16772097, DisponÃvel em, Acesso em: 06 fev. 2013Wei, A., Effects of cassava starch hydrolysate on cell growth and lipid accumulation of the heterotrophic microalgae Chlorella protothecoides (2009) Journal of Industrial Microbiology and Biotechnology, 36, pp. 1383-1389. , http://www.ncbi.nlm.nih.gov/pubmed/19633877, DisponÃvel em, Acesso em: 29 jan. 2010Knothe, G., Fuel properties (2005) The biodiesel handbook Champaign, 332p. , In: KNOTHE, G.et al. (Ed.). Illinois: AOC